Development and application of TaqMan probe‐based quantitative PCR assays for the detection of tilapia parvovirus

2021 ◽  
Author(s):  
Jidapa Yamkasem ◽  
Puntanat Tattiyapong ◽  
Win Surachetpong
Keyword(s):  
Quantitative Pcr ◽  
Taqman Probe ◽  
Pcr Assays

1020 Establishment of Taqman Probe-Based Quantitative PCR Assays for Evaluation of Bacterial Markers in Human Fecal Samples

2014 ◽  
Vol 146 (5) ◽  
pp. S-179-S-180
Author(s):  
Qiaoyi Liang ◽  
Tung On Yau ◽  
Francis K.L. Chan ◽  
Joseph J.Y. Sung ◽  
Jun Yu
Keyword(s):  
Quantitative Pcr ◽  
Taqman Probe ◽  
Fecal Samples ◽  
Pcr Assays

scholarly journals Application of Quantitative-PCR to Monitor Netpen Sites in British Columbia (Canada) for Tenacibaculum Species

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 414
Author(s):  
Joseph P. Nowlan ◽  
Scott R. Britney ◽  
John S. Lumsden ◽  
Spencer Russell
Keyword(s):  
Atlantic Salmon ◽  
Quantitative Pcr ◽  
Salmo Salar ◽  
Bacterial Load ◽  
Temporal Distribution ◽  
Quality Parameters ◽  
Antimicrobial Use ◽  
Live Fish ◽  
Dead Fish ◽  
Pcr Assays

Tenacibaculum are frequently detected from fish with tenacibaculosis at aquaculture sites; however, information on the ecology of these bacteria is sparse. Quantitative-PCR assays were used to detect T. maritimum and T. dicentrarchi at commercial Atlantic salmon (Salmo salar) netpen sites throughout several tenacibaculosis outbreaks. T. dicentrarchi and T. maritimum were identified in live fish, dead fish, other organisms associated with netpens, water samples and on inanimate substrates, which indicates a ubiquitous distribution around stocked netpen sites. Before an outbreak, T. dicentrarchi was found throughout the environment and from fish, and T. maritimum was infrequently identified. During an outbreak, increases in the bacterial load in were recorded and no differences were recorded after an outbreak supporting the observed recrudescence of mouthrot. More bacteria were recorded in the summer months, with more mortality events and antibiotic treatments, indicating that seasonality may influence tenacibaculosis; however, outbreaks occurred in both seasons. Relationships were identified between fish mortalities and antimicrobial use to water quality parameters (temperature, salinity, dissolved oxygen) (p < 0.05), but with low R2 values (<0.25), other variables are also involved. Furthermore, Tenacibaculum species appear to have a ubiquitous spatial and temporal distribution around stocked netpen sites, and with the potential to induce disease in Atlantic salmon, continued research is needed.

Reverse transcription-quantitative PCR assays for genotype-specific detection of human noroviruses in clinical and environmental samples

2018 ◽  
Vol 221 (3) ◽  
pp. 578-585 ◽  
Author(s):  
Mohan Amarasiri ◽  
Masaaki Kitajima ◽  
Akiho Miyamura ◽  
Ricardo Santos ◽  
Silvia Monteiro ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Environmental Samples ◽  
Specific Detection ◽  
Human Noroviruses ◽  
Reverse Transcription Quantitative Pcr ◽  
Pcr Assays

Practical Considerations for the Design of Quantitative PCR Assays

1995 ◽  
pp. 84-108 ◽  
Author(s):  
Robert Diaco
Keyword(s):  
Quantitative Pcr ◽  
Pcr Assays

Evaluation of Various Real-Time Reverse Transcription Quantitative PCR Assays for Norovirus Detection

2017 ◽  
Vol 27 (4) ◽  
pp. 816-824 ◽  
Author(s):  
Ju Eun Yoo ◽  
Cheonghoon Lee ◽  
SungJun Park ◽  
GwangPyo Ko
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Reverse Transcription Quantitative Pcr ◽  
Pcr Assays

Abstract 740: Rapid detection of IDH1/2 mutations using RNaseH2 dependent, multiplex quantitative PCR Assays

2017 ◽  
Author(s):  
Yun Bao ◽  
Aymen Baig ◽  
Yu Wang ◽  
A. John Iafrate ◽  
Caifu Chen ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Rapid Detection ◽  
Pcr Assays

scholarly journals Establishment and In-House Validation of Simplex and Duplex PCR Methods for Event-Specific Detection of Maize SYN-E3272-5 Using a New Reference Molecule

2010 ◽  
Vol 93 (2) ◽  
pp. 663-675
Author(s):  
Kailin Shen ◽  
Xiang Li ◽  
Shu Wang ◽  
Yingjie Pan ◽  
Zhiyi Shi ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Genetically Modified Organisms ◽  
Insertion Site ◽  
Quantitative Detection ◽  
Duplex System ◽  
Detection Techniques ◽  
Qualitative And Quantitative ◽  
Reference Molecule ◽  
Gm Maize ◽  
Pcr Assays

Abstract Despite rapid developments in the detection techniques for genetically modified organisms (GMOs), the event-specific PCR method with high specificity is still the most used technique. In this study, event-specific simplex and duplex qualitative and quantitative detection systems were developed targeting the 3 insertion site of GM maize SYN-E3272-5 (3272) construct. A reference molecule p3272 was constructed to act as positive control and as calibrator for quantitative analysis. The LOD for simplex and duplex qualitative PCR assays was 10 copies of p3272 control DNA. LOD and the LOQ for simplex and duplex quantitative PCR assays were 10 and 25 copies of p3272 DNA, respectively. Furthermore, four practical GM maize samples were quantified using the established simplex and duplex quantitative PCR systems by in-house validation. Results from five operators showed that the bias ranged from 3.44 to 17.24 in the simplex system and from 0.42 to 16.06 in the duplex system, respectively. These results demonstrated that the established event-specific simplex and duplex qualitative and quantitative PCR systems combined with the reference molecule p3272 are suitable for the detection of GM maize 3272 and its derived products.

scholarly journals 20β-hydroxysteroid dehydrogenase and CYP19A1 are differentially expressed during maturation in Atlantic cod (Gadus morhua)

2007 ◽  
Vol 39 (4) ◽  
pp. 319-328 ◽  
Author(s):  
C Mittelholzer ◽  
E Andersson ◽  
D Consten ◽  
T Hirai ◽  
Y Nagahama ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
18S Rrna ◽  
Gadus Morhua ◽  
Atlantic Cod ◽  
Elongation Factor ◽  
Hydroxysteroid Dehydrogenase ◽  
Elongation Factor 1Α ◽  
Eukaryotic Elongation Factor ◽  
Pcr Assays

AbstractIn order to better quantify the molecular mechanisms regulating final oocyte maturation and spawning, complete coding sequences with partially or fully untranslated regions for the steroidogenic enzymes, cytochrome P450 aromatase and 20β-hydroxysteroid dehydrogenase, were cloned from ovaries of Atlantic cod (Gadus morhua). The nucleotide and amino acid sequences showed high homologies with the corresponding sequences of other fish species, and conserved features important for functionality were identified in both predicted proteins. The sequences of the corresponding genomic loci were also determined, allowing the design of mRNA-specific quantitative PCR assays. As a reference gene for the real-time RT-PCR assays, eukaryotic elongation factor 1α was chosen, and the mRNA as well as the genomic sequence was determined. In addition, a real-time quantitative PCR assay for the 18S rRNA was adapted to be used in cod. Analysis of immature and maturing female cod from July to January respectively showed that the enzyme genes showed the expected quantitative changes associated with physiological regulation. However, mRNA for eukaryotic elongation factor 1α, and to a lesser extent even 18S rRNA, showed variable expression in these samples as well. To find accurate standards for real-time PCR in such a dynamic organ as the cod ovary is not an easy task, and several possible solutions are discussed.

Sign in / Sign up

Export Citation Format

Share Document