scholarly journals Extracellular vesicles miR‐210 as a potential biomarker for diagnosis and survival prediction of oral squamous cell carcinoma patients.

2021 ◽  
Author(s):  
Elisabetta Bigagli ◽  
Luca Giovanni Locatello ◽  
Arianna Di Stadio ◽  
Giandomenico Maggiore ◽  
Francesca Valdarnini ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Extracellular Vesicles ◽  
Squamous Cell ◽  
Survival Prediction ◽  
Potential Biomarker

scholarly journals Cisplatin-Resistance in Oral Squamous Cell Carcinoma: Regulation by Tumor Cell-Derived Extracellular Vesicles

Cancers ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1166 ◽  
Author(s):  
Xin-Hui Khoo ◽  
Ian C. Paterson ◽  
Bey-Hing Goh ◽  
Wai-Leng Lee
Keyword(s):  
Drug Resistance ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Extracellular Vesicles ◽  
Squamous Cell ◽  
Metal Ion ◽  
De Novo ◽  
Protein Profile ◽  
Severe Problem

Drug resistance remains a severe problem in most chemotherapy regimes. Recently, it has been suggested that cancer cell-derived extracellular vesicles (EVs) could mediate drug resistance. In this study, the role of EVs in mediating the response of oral squamous cell carcinoma (OSCC) cells to cisplatin was investigated. We isolated and characterized EVs from OSCC cell lines showing differential sensitivities to cisplatin. Increased EV production was observed in both de novo (H314) and adaptive (H103/cisD2) resistant lines compared to sensitive H103 cells. The protein profiles of these EVs were then analyzed. Differences in the proteome of EVs secreted by H103 and H103/cisD2 indicated that adaptation to cisplatin treatment caused significant changes in the secreted nanovesicles. Intriguingly, both resistant H103/cisD2 and H314 cells shared a highly similar EV protein profile including downregulation of the metal ion transporter, ATP1B3, in the EVs implicating altered drug delivery. ICP-MS analysis revealed that less cisplatin accumulated in the resistant cells, but higher levels were detected in their EVs. Therefore, we inhibited EV secretion from the cells using a proton pump inhibitor and observed an increased drug sensitivity in cisplatin-resistant H314 cells. This finding suggests that control of EV secretion could be a potential strategy to enhance the efficacy of cancer treatment.

Animal model and bioinformatics analyses suggest the TIMP1/MMP9 axis as a potential biomarker in oral squamous cell carcinoma

2020 ◽  
Vol 59 (11) ◽  
pp. 1302-1316
Author(s):  
Guoqiang Xu ◽  
Jianing Wei ◽  
Bing Huangfu ◽  
Jiping Gao ◽  
Xiaotang Wang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Animal Model ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Bioinformatics Analyses ◽  
Potential Biomarker

Role of pretreatment neutrophil-to-lymphocyte ratio as an independent prognostic factor in oral squamous cell carcinoma patients: A prospective study in a tertiary care center

2018 ◽  
Vol 3 (2) ◽  
pp. 37
Author(s):  
Shashwat Tiwari ◽  
Shivani Nautiyal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tertiary Care ◽  
Prognostic Index ◽  
Prognostic Scores ◽  
Lymphocyte Ratio ◽  
Potential Biomarker ◽  
The Absolute

<p><strong>Background:</strong> More recently, established systemic inflammation-based prognostic scores have been explored extensively, such as neutrophil-lymphocyte ratio (NLR) and serum C-reactive protein. We postulated that NLR might be a readily available and inexpensive objective prognostic index that could be used in daily oncologic clinical practice and could help to stratify patients in clinical trials.<br /><strong>Materials and Methods:</strong> In total, there were 150 patients with oral squamous cell carcinoma (OSCC) treated at GSVM Medical College, Kanpur, between October 2012 and January 2015 whose clinical information and laboratory parameters were obtained. The NLR was determined by dividing the absolute neutrophil count by the absolute lymphocyte count, and the NLR data were then dichotomized and divided into two groups as NLR low and high. <strong>Conclusion:</strong> Our findings reported herein demonstrated that pretreatment NLR is a potential biomarker for predicting the overall survival in OSCC patients. Combined with other markers, NLR may be used in decision-making and the selection of treatment modality in patients with oral SCC.</p>

scholarly journals Overexpression of Stathmin in Oral Squamous Cell Carcinoma, its Association with Histomorphological Features and Pathological Staging

2021 ◽  
Author(s):  
Nabanita Barma ◽  
Gopinath Barui ◽  
Anadi Roy Chowdhury
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cancer Progression ◽  
Squamous Cell ◽  
Tertiary Care ◽  
Clinicopathological Parameters ◽  
Pathological Staging ◽  
Potential Biomarker ◽  
Tobacco Chewing

Introduction: Stathmin, one of the most important intracellular phosphoprotein that plays the vital role in the regulation of cell cycle and cell proliferation. It acts by disassembly of Microtubules (MT) that helps in the formation of mitotic spindle. It is overexpressed in various human cancers. Aim: To investigate the expression status of stathmin and its relation with clinicopathologic significance in Oral Squamous Cell Carcinoma (OSCC) and its association with age, sex, tobacco chewing, histomorphological features and pathological staging. Materials and Methods: A descriptive and observational study in cross-sectional design was conducted in a tertiary care centre of Kolkata, West Bengal, India, from January 2020 to March 2020 in the Department of Pathology of RG Kar Medical College and Hospital in collaboration with Department of Oto-Rhino-Laryngology of the same institute. Stathmin expression was assessed by immunohistochemistry (IHC) in 28 OSCC cases. The association between stathmin expression and clinicopathological parameters like age, sex, tobacco chewing, tumour site, histomorphological type, Tumor Nodes Metastases (TNM) stage and Worst Pattern Of Invasion (WPOI) were evaluated on the basis of Chi-square test and Fisher’s-exact test (software Statistical Package for the Social Sciences (SPSS) V 25.0). Results: Stathmin was overexpressed in 18 cases out of 28 cases of OSCC. In this study a positive association was observed between stathmin expression and age group, history of tobacco chewing, advanced T stage, advanced pTNM staging and WPOI of OSCC. Conclusion: The study suggests that overexpression of stathmin could contribute to cancer progression. There is a higher likelihood that stathmin may be used as a potential biomarker as well as therapeutic target for OSCC.

Cripto 1, a potential biomarker for oral squamous cell carcinoma

Oral Oncology ◽  
2019 ◽  
Vol 90 ◽  
pp. 153-154 ◽  
Author(s):  
Anu Jain ◽  
Sumanth Mallupattu ◽  
Reetu Thakur ◽  
Sushmita Ghoshal ◽  
Arnab Pal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Potential Biomarker

Significance of molecular markers in survival prediction of oral squamous cell carcinoma

Head & Neck ◽  
2011 ◽  
Vol 34 (7) ◽  
pp. 929-936 ◽  
Author(s):  
Ki-Yeol Kim ◽  
ShengJin Li ◽  
Jeong-Dan Cha ◽  
Xianglan Zhang ◽  
In-Ho Cha
Keyword(s):  
Squamous Cell Carcinoma ◽  
Molecular Markers ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Survival Prediction

scholarly journals HISTOPATHOLOGICAL GRADING OF ORAL SQUAMOUS CELL CARCINOMA BY MICRONUCLEI ASSAY, A POTENTIAL BIOMARKER IN ORAL EXFOLIATED CELLS

2021 ◽  
pp. 95-99
Author(s):  
Noratan Jatiya ◽  
Vijay Rajak ◽  
Savita Soni
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tobacco Consumption ◽  
Control Group ◽  
Exfoliated Cells ◽  
Potential Biomarker ◽  
The Difference ◽  
Histopathological Grading

Background: Micronucleus is a microscopically visible round or oval cytoplasmic chromatin mass in the extra nuclear vicinity, originated from aberrant mitosis, which consists of eccentric chromosomes that have failed to reach spindle poles during mitosis and are used as biomarkers for assessment of DNA damage. Micronuclei in exfoliated oral epithelial cells have been shown in some studies to correlate with severity of this genotoxic damage. This severity can be measured in terms of grading of the lesions. Aim: To correlate frequency of micronuclei (MN) in oral exfoliated cells in clinically diagnosed cases of oral squamous cell carcinoma (OSCC) followed by a histopathological grading. Materials and Methods : The study subjects consisted of clinically diagnosed cases of OSCC. Healthy subjects without any tobacco consumption habits formed the control group.The cytosmears from both groups were stained with rapid Papanicolaou stain. MN were identified according to the criteria given by Tolbert et al. Results: The frequency of MN was three to four times higher in patients with OSCC as compared to patients in the control group and the difference was found to be highly significant. In 75% cases, the cytological grade as determined by the frequency of micronuclei correlated with the histopathological grade and this observation was statistically significant. Conclusions : The MN is potentially an excellent candidate to serve as such a biomarker for prediction of the grade of OSCC.

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