Efficacy of biosynthesized silver nanoparticles from Larrea tridentata against Clavibacter michiganensis

2021 ◽  
Author(s):  
Rolando Méndez‐Andrade ◽  
Moises Roberto Vallejo‐Perez ◽  
Esperanza Loera‐Alvarado ◽  
Gladys los Santos‐Villarreal ◽  
Luis Alfonso García‐Cerda ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Larrea Tridentata ◽  
Clavibacter Michiganensis

Bactericidal effect of silver nanoparticles against propagation of Clavibacter michiganensis infection in Lycopersicon esculentum Mill

2018 ◽  
Vol 115 ◽  
pp. 358-362 ◽  
Author(s):  
Raymundo Rene Rivas-Cáceres ◽  
Jose Luis Stephano-Hornedo ◽  
Jorge Lugo ◽  
Rocio Vaca ◽  
Pedro Del Aguila ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Lycopersicon Esculentum ◽  
Bactericidal Effect ◽  
Clavibacter Michiganensis ◽  
Lycopersicon Esculentum Mill

scholarly journals Antimicrobial activities of bio-synthesized silver nanoparticles on strains of clavibacter michiganensis subsp. michiganensis

2019 ◽  
Vol 37 (2) ◽  
pp. 1-10
Author(s):  
A. R. Oloyede ◽  
O. T. Familola ◽  
O. I. Sonde ◽  
J. O. Ossai ◽  
A. O. Wahab
Keyword(s):  
Silver Nanoparticles ◽  
Inhibitory Activity ◽  
Tomato Plant ◽  
Antibacterial Activities ◽  
Clavibacter Michiganensis ◽  
Ag Nps ◽  
Canker Disease ◽  
Clavibacter Michiganensis Subsp ◽  
Zones Of Inhibition ◽  
Uv Visible

Silver nanoparticles (Ag-NPs) have been known to possess unique antimicrobial properties which help in medicine and water treatment, but their potentials in agriculture have not been fully utilized. This study was conducted to determine the inhibitory activity of bio-synthesized Silver nanoparticles on strains of Clavibacter michiganensis subsp. Michiganensis, causative agents of canker disease of tomato plant. The Ag-NPs were synthesized using Cladosporium cladosporioides, leaf extracts of Azadirachta indica and Vernonia amygdalina. The synthesized silver nanoparticles were characterized using UV-visible absorption spectroscopy. The antibacterial activities of bio-synthesized Ag-NPs on strains of C. michiganensis subsp. michiganensis were evaluated using agar well diffusion method. UV-visible spectroscopic analysis revealed rapid reduction of silver ions by C. cladosporioides, A. indica and V. amygdalina extracts where surface Plasmon absorption maxima were observed at 400nm – 450nm from the UV-visible spectra. The in-vitro antibacterial activities revealed that Ag-NPs synthesized using V. amygdalina and A. indica extracts inhibited the growth of C. michiganensis subsp. michiganensis strains with zones of inhibition ranging from 15.70 mm - 24.70mm and 21.00mm – 30.00mm, respectively, while those synthesized using C. cladosporioides showed zones of inhibition ranging from 0.00mm – 12.00mm. Growth curves of C. michiganensis subsp. michiganensis strains in the presence of biosynthesized Ag-NPs showed inhibition of growths after 4 – 10 hours of exposure. The results of this study indicated that the bio-synthesized silver nanoparticles could be effective in controlling bacterial canker disease of tomato plant caused by strains of C. michiganensis subsp. michiganensis thereby reducing the toxic effects of chemical bactericides on important agricultural products.Keywords: Silver nanoparticles; inhibitory activity; canker, tomato; zones of inhibition

Antibacterial Effect of Silver Nanoparticles (AgNPs) Synthesized from Trichoderma Harzianum against Clavibacter Michiganensis

2019 ◽  
Vol 58 ◽  
pp. 10-19 ◽  
Author(s):  
Asma Noshad ◽  
Mudassar Iqbal ◽  
Laura Folkers ◽  
Crispin Hetherington ◽  
Aamir Khan ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Trichoderma Harzianum ◽  
Bacterial Pathogen ◽  
Diffusion Method ◽  
Scale Production ◽  
Clavibacter Michiganensis ◽  
Disk Diffusion Method ◽  
Canker Disease ◽  
20 Nm

In nanotechnology, fungal mediated green synthesis of silver nanoparticles (AgNPs) has tremendous application in the development of antimicrobial systems but the mechanism behind the synthesis is yet to be understood. This study aims to synthesize the silver nanoparticlesviaa green chemistry route using mycellial aqueous extract of agriculturally beneficial fungiTrichoderma harzianum.Two different concentrations (1 mM and 2.5 Mm) of aqueous silver salt (AgNO3) were used and mixed as 1:1 ratio with aqueous extract ofT. harzianumat room temperature and the pH of the reaction mixture was monitored until it stabilized.Formation of AgNPs was confirmed by using UV-Vis spectroscopic analysis. For further insight, AgNPs were characterized by using HR-TEM and XRD, which clearly showed appearance of crystalline, monodispersed round-shaped particles of 3-20 nm in size. The synthesised NPs were subjected to antimicrobial assay against gram +ve and gram –ve bacteria using the disk diffusion method. The focused species wasClavibactermichiganensis subsp michiganensis,which is the causitive pathogen of Tomato canker disease and we hypothesised that the synthesised AgNPs might be useful to control this pathogen. Appreciable antibiotic activity was monitored even at a low concentration of 1mM level, while the zone of inhibition was positively increased at 2.5 mM. Our results clearly indicate that the present process is an excellent candidate for industrial scale production of AgNPs, and has the potential to control the bacterial pathogencmm.

scholarly journals Bactericidal Activity of Larrea tridentata Hydroalcoholic Extract against Phytopathogenic Bacteria

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 957
Author(s):  
Ana Lizet Morales-Ubaldo ◽  
Nallely Rivero-Perez ◽  
Fidel Avila-Ramos ◽  
Eliazar Aquino-Torres ◽  
Judith Prieto-Méndez ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Pseudomonas Syringae ◽  
Bactericidal Activity ◽  
Xanthomonas Campestris ◽  
Larrea Tridentata ◽  
Minimal Bactericidal Concentration ◽  
Organic Fraction ◽  
Clavibacter Michiganensis ◽  
Hydroalcoholic Extract ◽  
Phytopathogenic Bacteria

Due to the emergence of bacterial resistance in phytopathogenic microorganisms, it is necessary to search for new treatment alternatives for these pathogens. Natural extracts are a potential source of bioactive compounds that can act against such bacterial strains. The antibacterial activity of Larrea tridentata against bacteria with public health importance has been documented; however, few reports cover pathogens associated with the agricultural sector. The aim of the present study was to evaluate the antibacterial activity of Larrea tridentata hydroalcoholic extract (LTHE) and fractions against phytopathogenic bacteria. LTHE was obtained by the maceration technique and then subjected to bipartition using solvents of different polarities. Clavibacter michiganensis sbsp. michiganensis, Pseudomonas syringae, and Xanthomonas campestris strains were used, and their antibiotic sensitivity was determined. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of LTHE and its fractions (aqueous: LTAq-F; organic: LTEtOAc-F) were determined. LTHE and its organic fraction showed bactericidal activity against the three bacteria, showing better activity against X. campestris, exhibiting an MIC of 0.39 mg/mL and an MBC of 0.78 mg/mL. The results show that LTHE and its organic fraction have bactericidal activity in vitro against Clavibacter michiganensis sbsp. michiganensis, Pseudomonas syringae, and Xanthomonas campestris.

Synthesis, performance and growth mechanism of silver nanoparticle coated SERS fiber probe

2019 ◽  
Vol 107 (3) ◽  
pp. 305
Author(s):  
Mengmei Geng ◽  
Yuting Long ◽  
Tongqing Liu ◽  
Zijuan Du ◽  
Hong Li ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Absorption Spectrum ◽  
Reaction Time ◽  
Growth Mechanism ◽  
Fiber Optic ◽  
Fiber Optic Probe ◽  
Visible Absorption ◽  
Fiber Probe ◽  
Surface Enhanced Raman ◽  
Optic Probe

Surface-enhanced Raman Scattering (SERS) fiber probe provides abundant interaction area between light and materials, permits detection within limited space and is especially useful for remote or in situ detection. A silver decorated SERS fiber optic probe was prepared by hydrothermal method. This method manages to accomplish the growth of silver nanoparticles and its adherence on fiber optic tip within one step, simplifying the synthetic procedure. The effects of reaction time on phase composition, surface plasmon resonance property and morphology were investigated by X-ray diffraction analysis (XRD), ultraviolet-visible absorption spectrum (UV-VIS absorption spectrum) and scanning electron microscope (SEM). The results showed that when reaction time is prolonged from 4–8 hours at 180 °C, crystals size and size distribution of silver nanoparticles increase. Furthermore, the morphology, crystal size and distribution density of silver nanoparticles evolve along with reaction time. A growth mechanism based on two factors, equilibrium between nucleation and growth, and the existence of PVP, is hypothesized. The SERS fiber probe can detect rhodamin 6G (R6G) at the concentration of 10−6 M. This SERS fiber probe exhibits promising potential in organic dye and pesticide residue detection.

Assessment of the bactericidal effect of green synthesized silver nanoparticles against a panel of infectious microorganisms

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
E Antunes ◽  
M Mmola ◽  
M Meyer ◽  
DR Beukes
Keyword(s):  
Silver Nanoparticles ◽  
Bactericidal Effect ◽  
Green Synthesized Silver Nanoparticles

The Effect of Silver Nanoparticles on Blood Cells in Rats

2012 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Rezaei-Zarchi ◽  
M. Taghavi-Foumani ◽  
S. Razavi Sheshdeh ◽  
M. Negahdary ◽  
G. Rahimi
Keyword(s):  
Silver Nanoparticles ◽  
Blood Cells

Antimicrobial Efficacy of Green Silver Nanoparticles and Potential Implications for Human Health and the Environment

2015 ◽  
Vol 1 (2) ◽  
pp. 81-90 ◽  
Author(s):  
Mohsin Khan ◽  
Mh Aftab ◽  
Varun Chauhan ◽  
Jyotsna Kaushal
Keyword(s):  
Silver Nanoparticles ◽  
Human Health ◽  
Antimicrobial Efficacy
Sign in / Sign up

Export Citation Format

Share Document