Selection of ACC deaminase positive, thermohalotolerant and drought tolerance enhancing plant growth‐promoting bacteria from rhizospheres of Cyamopsis tetragonoloba grown in arid regions

In addition to the well-known roles of indoleacetic acid and cytokinin in crown gall formation, the plant hormone ethylene also plays an important role in this process. Many plant growth-promoting bacteria (PGPB) encode the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which can degrade ACC, the immediate precursor of ethylene in plants, to α-ketobutyrate and ammonia and thereby lower plant ethylene levels. To study the effect of ACC deaminase on crown gall development, an ACC deaminase gene from the PGPB Pseudomonas putida UW4 was introduced into Agrobacterium tumefaciens C58, so that the effect of ACC deaminase activity on tumour formation in tomato and castor bean plants could be assessed. Plants were also coinoculated with A. tumefaciens C58 and P. putida UW4 or P. putida UW4-acdS– (an ACC deaminase minus mutant strain). In both types of experiments, it was observed that the presence of ACC deaminase generally inhibited tumour development on both tomato and castor bean plants.

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Growth response of wheat cultivars to bacterial inoculation in calcareous soil

Plant Soil and Environment ◽

10.17221/75/2010-pse ◽

2010 ◽

Vol 56 (No. 12) ◽

pp. 570-573 ◽

Cited By ~ 14

Author(s):

D. Egamberdieva

Keyword(s):

Plant Growth ◽

Calcareous Soil ◽

Dry Weight ◽

Plant Growth Promoting Bacteria ◽

Wheat Cultivars ◽

Bacterial Strains ◽

Bacterial Inoculation ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Selection Of

In this study the plant growth-promoting bacteria were analysed for their growth-stimulating effects on two wheat cultivars. The investigations were carried out in pot experiments using calcareous soil. The results showed that bacterial strains Pseudomonas spp. NUU1 and P. fluorescens NUU2 were able to colonize the rhizosphere of both wheat cultivars. Their plant growth-stimulating abilities were affected by wheat cultivars. The bacterial strains Pseudomonas sp. NUU1 and P. fluorescens NUU2 significantly stimulated the shoot and root length and dry weight of wheat cv. Turon, whereas cv. Residence was less affected by bacterial inoculation. The results of our study suggest that inoculation of wheat with Pseudomonas strains can improve plant growth in calcareous soil and it depends upon wheat cultivars. Prior to a selection of good bacterial inoculants, it is recommended to select cultivars that benefit from association with these bacteria.

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Levels of ACC and related compounds in exudate and extracts of canola seeds treated with ACC deaminase-containing plant growth-promoting bacteria

Canadian Journal of Microbiology ◽

10.1139/w01-014 ◽

2001 ◽

Vol 47 (4) ◽

pp. 368-372 ◽

Cited By ~ 134

Author(s):

Donna M Penrose ◽

Bernard R Glick

Keyword(s):

Plant Growth ◽

Acc Deaminase ◽

Aminobutyric Acid ◽

Plant Growth Promoting Bacteria ◽

Canola Seed ◽

Test Part ◽

Seed Exudate ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Related Compounds

It was previously proposed that plant growth-promoting bacteria that possess 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase could utilize ACC that is present in the exudate of germinating canola seeds. The uptake and cleavage of ACC by these bacteria would lower the level of ACC, and thus ethylene within the plant, and reduce the extent of its inhibition on root elongation. To test part of the above mentioned model, ACC levels were monitored in canola seed tissues and exudate during germination. Lower amounts of ACC were present in the exudate and tissues of seeds treated with the plant growth-promoting bacterium Enterobacter cloacae CAL3, than in control seeds treated with MgSO4. The ACC-related compounds, α- and γ-aminobutyric acids, both known to stimulate ethylene production, were also measured in the canola seed exudate and tissues. Approximately the same levels of α-aminobutyric acid were present in the exudates of the bacterium-treated seeds and the control seeds, but the amount of α-aminobutyric acid was lower in the tissues of the bacterium-treated seeds than in the control seeds. Smaller quantities of γ-aminobutyric acid were seen in both the exudate and tissues of the E. cloacae CAL3-treated seeds than in the control seeds.Key words: ACC ethylene, canola, seed extract, seed exudate, plant growth-promoting bacteria.

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Involvement ofgacSandrpoSin enhancement of the plant growth-promoting capabilities ofEnterobacter cloacaeCAL2 and UW4

Canadian Journal of Microbiology ◽

10.1139/w01-072 ◽

2001 ◽

Vol 47 (8) ◽

pp. 698-705 ◽

Cited By ~ 90

Author(s):

Saleema S Saleh ◽

Bernard R Glick

Keyword(s):

Plant Growth ◽

Pseudomonas Fluorescens ◽

Indoleacetic Acid ◽

Acc Deaminase ◽

Enterobacter Cloacae ◽

Lag Phase ◽

Plant Growth Promoting Bacteria ◽

Multicopy Plasmid ◽

Plant Growth Promoting ◽

Growth Promoting

The plant growth-promoting bacteria Enterobacter cloacae CAL2 and UW4 were genetically transformed with a multicopy plasmid containing an rpoS or gacS gene from Pseudomonas fluorescens. The transformed strains were compared with the nontransformed strains for growth, indoleacetic acid (IAA) production, antibiotic production, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, siderophore production, cell morphology, and the ability to promote canola root elongation. All transformed strains had a longer lag phase, were slower in reaching stationary phase, and attained a higher cell density than the nontransformed strains. Transformation resulted in cells that were significantly shorter than the nontransformed cells. The transformed strains also produced significantly more IAA than the nontransformed strains. Introduction of rpoS or gacS from Pseudomonas fluorescens was associated with a reduction in the production of both antibiotics, 2,4-diacetylphloroglucinol and mono-acetylphloroglucinol, produced by Enterobacter cloacae CAL2. With Enterobacter cloacae CAL2, plasmid-borne rpoS, but not gacS, increased the level of ACC deaminase activity, while introduction of rpoS in Enterobacter cloacae UW4 caused a decrease in ACC deaminase activity. Neither gacS nor rpoS significantly affected the level of siderophores synthesized by either bacterial strain. Overproduction of either GacA or RpoS in Enterobacter cloacae CAL2 resulted in a significant increase in the root lengths of canola seedlings when seeds were treated with the bacteria, and overproduction of RpoS caused an increase in canola shoot as well as root lengths.Key words: plant growth-promoting bacteria, canola, ethylene, ACC deaminase, GacS, RpoS, indoleacetic acid, siderophores, antibiotics.

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Interaction between sugarcane families and plant growth-promoting bacteria in two crop cycles

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n2p527 ◽

2019 ◽

Vol 40 (2) ◽

pp. 527 ◽

Cited By ~ 2

Author(s):

Valéria Rosa Lopes ◽

João Carlos Bespalhok Filho ◽

Guilherme Grodzki Oliveira Figueiredo ◽

Ricardo Augusto de Oliveira ◽

Edelclaiton Daros

Keyword(s):

Plant Growth ◽

Average Height ◽

Plant Growth Promoting Bacteria ◽

Plant Genotype ◽

Saccharum Spp ◽

Stalk Diameter ◽

Plant Growth Promoting ◽

Azospirillum Amazonense ◽

Growth Promoting ◽

Selection Of

The response of sugarcane (Saccharum spp.) families to the inoculation with plant growth-promoting bacteria (PGPB) may show positive or negative interaction values depending on the plant genotype, allowing the selection of responsive genotypes to the association. Based on these precepts, this study aimed to evaluate the response of 27 sugarcane families treated with two PGPB-based inoculants in two sugarcane cycles: plant-cane and first ratoon. Four months after germination, 27 families from crosses between clones of sugarcane were: (T0) not inoculated; (T1) inoculated with Triazo, which consists of a mixture of the strains Abv5, Abv6, and Abv7 of Azospirillum brasilense; and (T2) inoculated with an inoculant called Mix, which contains five strains of PGPB (Gluconacetobacter diazotrophicus Pal5, Azospirillum amazonense CBAmC, Burkholderia tropica Ppe8, Herbaspirillum rubrisubalbicans HCc103, and Herbaspirillum seropedicae HRC54). The productivity traits evaluated were the number of stalks, average height, stalk diameter, and Brix. The correlation values between the two cycles were 0.87 for the number of stalks, 0.70 for average height, 0.48 for stalk diameter, and 0.63 for Brix. Differences were observed between treatments for average height, stalk diameter, and Brix. Differences were also observed between families for all the studied traits. Brix was the only parameter that presented a significant interaction between family and inoculant. Different responses were observed between cycles for some families, showing that other factors can also influence the association.

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Induction of drought tolerance in tomato upon the application of ACC deaminase producing plant growth promoting rhizobacterium Bacillus subtilis Rhizo SF 48

Microbiological Research ◽

10.1016/j.micres.2020.126422 ◽

2020 ◽

Vol 234 ◽

pp. 126422 ◽

Cited By ~ 2

Author(s):

Gowtham H.G. ◽

Brijesh Singh S. ◽

Murali M. ◽

Shilpa N. ◽

Melvin Prasad ◽

...

Keyword(s):

Bacillus Subtilis ◽

Drought Tolerance ◽

Plant Growth ◽

Acc Deaminase ◽

Plant Growth Promoting ◽

Growth Promoting

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Determination of 1-aminocycopropane-1-carboxylic acid (ACC) to assess the effects of ACC deaminase-containing bacteria on roots of canola seedlings

Canadian Journal of Microbiology ◽

10.1139/w00-128 ◽

2001 ◽

Vol 47 (1) ◽

pp. 77-80 ◽

Cited By ~ 126

Author(s):

Donna M Penrose ◽

Barbara A Moffatt ◽

Bernard R Glick

Keyword(s):

Carboxylic Acid ◽

Plant Growth ◽

Root Elongation ◽

Acc Deaminase ◽

Plant Growth Promoting Bacteria ◽

Psychrophilic Bacterium ◽

Ethylene Inhibitor ◽

Seedling Roots ◽

Plant Growth Promoting ◽

Growth Promoting

Previously, it was proposed that plant growth-promoting bacteria that possess the enzyme, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, can reduce the amount of ethylene produced by a plant and thereby promote root elongation. To test this model, canola seeds were imbibed in the presence of the chemical ethylene inhibitor, 2-aminoethoxyvinyl glycine (AVG), various strains of plant growth-promoting bacteria, and a psychrophilic bacterium containing an ACC deaminase gene on a broad host range plasmid. The extent of root elongation and levels of ACC, the immediate precursor of ethylene, were measured in the canola seedling roots. A modification of the Waters AccQ*Tag Amino Acid Analysis Method(tm) was used to quantify ACC in the root extracts. It was found that, in the presence of the ethylene inhibitor, AVG, or any one of several ACC deaminase-containing strains of bacteria, the growth of canola seedling roots was enhanced and the ACC levels in these roots were lowered.

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