Growth response of wheat cultivars to bacterial inoculation in calcareous soil

In this study the plant growth-promoting bacteria were analysed for their growth-stimulating effects on two wheat cultivars. The investigations were carried out in pot experiments using calcareous soil. The results showed that bacterial strains Pseudomonas spp. NUU1 and P. fluorescens NUU2 were able to colonize the rhizosphere of both wheat cultivars. Their plant growth-stimulating abilities were affected by wheat cultivars. The bacterial strains Pseudomonas sp. NUU1 and P. fluorescens NUU2 significantly stimulated the shoot and root length and dry weight of wheat cv. Turon, whereas cv. Residence was less affected by bacterial inoculation. The results of our study suggest that inoculation of wheat with Pseudomonas strains can improve plant growth in calcareous soil and it depends upon wheat cultivars. Prior to a selection of good bacterial inoculants, it is recommended to select cultivars that benefit from association with these bacteria.

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Plant growth-promoting bacteria belonging to the genera Pseudomonas and Bacillus improve the growth of sorghum seedings in a low-nutrient soil

Botanical Sciences ◽

10.17129/botsci.2841 ◽

2021 ◽

Vol 1 (1) ◽

Author(s):

Enriqueta Amora-Lazcano ◽

Héctor J. Quiroz-González ◽

Cristofer I. Osornio-Ortega ◽

Juan A. Cruz-Maya ◽

Janet Jan-Roblero

Keyword(s):

Plant Growth ◽

Growth Parameters ◽

Dry Weight ◽

Plant Growth Promoting Bacteria ◽

Phosphate Solubilizing Bacteria ◽

Bacterial Strains ◽

Shoot Dry Weight ◽

Soil Bioassay ◽

Plant Growth Promoting ◽

Growth Promoting

Background: Deficiency in sorghum growth in ecosystems of low-nutrient soils has been scarcely studied. This soil deficiency can be overcome by the addition of plant growth-promoting bacteria which increase sorghum growth. Questions and/or Hypotheses: indole acetic acid (IAA) producing and phosphate solubilizing bacteria can promote sorghum growth under nutritional stress. Studied species: Sorghum bicolor (L.) Moench. Study site and dates: Mexico City, 2018. Methods: Of the twelve bacterial strains utilized, three produce IAA (group BI), two strains produce IAA and siderophores (BIS group), four strains produce IAA and solubilize phosphate (BIP group), and three strains produce IAA, solubilize phosphate, and produce siderophores (BIPS group). Hydroponic bioassays and low-nutrient soil bioassay were used. Results: In hydroponic bioassays, for BI and BIS groups, five strains significantly increased the growth parameters with respect to the control, and for the BIP and BIPS groups, two strains promoted stem development and shoot dry weight. In a low-nutrient soil bioassay, Pseudomonas sp. BI-1 (from BI group) was the one that presented the highest percentages 32, 48, 140 and 79 % in stem diameter, height and dry weight of the shoot and dry weight of the root, respectively, followed by the P. mohnii BIPS-10 strain (from BIPS group) that exhibited similar results. Conclusions: IAA producing Pseudomonas strains improve the sorghum growth in a low-nutrient soil and suggest thatPseudomonas sp. BI-1 and P. mohnii BIPS-10 could be used as potential bioinoculants for sorghum.

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Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Plants ◽

10.3390/plants10050912 ◽

2021 ◽

Vol 10 (5) ◽

pp. 912

Author(s):

Shuming Liu ◽

Hongmei Liu ◽

Rui Chen ◽

Yong Ma ◽

Bo Yang ◽

...

Keyword(s):

Plant Growth ◽

Contaminated Soils ◽

Translocation Factor ◽

Plant Growth Promoting Bacteria ◽

Bacterial Strains ◽

Pgp Traits ◽

Cd Tolerance ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Cd Translocation

Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.

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The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽

10.3390/agronomy11050888 ◽

2021 ◽

Vol 11 (5) ◽

pp. 888

Author(s):

Giorgia Novello ◽

Patrizia Cesaro ◽

Elisa Bona ◽

Nadia Massa ◽

Fabio Gosetti ◽

...

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Global Market ◽

Plant Growth Promoting Bacteria ◽

Bacterial Strains ◽

Mineral Concentration ◽

Positive Effects ◽

Plant Growth Promoting ◽

Onion Cultivar ◽

Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

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Selection of Saccharum spp. rhizobacteria with growth-promoting properties using PCA analysis

Australian Journal of Crop Science ◽

10.21475/ajcs.20.14.07.p2698 ◽

2020 ◽

pp. 1186-1194

Author(s):

Roberta Mendes dos Santos ◽

Everlon Cid Rigobelo

Keyword(s):

Plant Growth ◽

Principal Component ◽

Plant Growth Promoting Rhizobacteria ◽

Cellulolytic Activity ◽

Bacterial Strains ◽

Saccharum Spp ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Enterobacter Asburiae ◽

Selection Of

The search for plant growth-promoting rhizobacteria is an ongoing need for the development of new bioinoculants for use in various crops, including sugarcane. Bacterial strains with various plant growth-promoting properties can contribute to sustainable agricultural production. The present study aimed to isolate, characterize and select sugarcane rhizobacteria from six different varieties through principal components analysis. This study selected 167 bacterial strains with the ability to fix nitrogen, produce indolacetic acid, exhibit cellulolytic activity, and solubilize phosphate and potassium were isolated. Of these 167 bacterial strains, seven were selected by principal component analysis and identified as belonging to the genera Staphylococcus, Enterobacter, Bacillus and Achromobacter. Bacillus thuringiensis IP21 presented higher potential for nitrogen fixation and CaPO4 and AlPO4 solubilization and a lower potential for K solubilization in sugarcane. Enterobacter asburiae IP24 was efficient in indolacetic acid production and CaPO4 and FePO4 solubilization and inefficient for Araxá apatite solubilization.

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Synergistic and offset effects of fungal species combinations on plant performance

10.1101/2021.05.09.443080 ◽

2021 ◽

Author(s):

Yoshie Hori ◽

Hiroaki Fujita ◽

Kei Hiruma ◽

Kazuhiko Narisawa ◽

Hirokazu Toju

Keyword(s):

Plant Growth ◽

Synergistic Effects ◽

Dry Weight ◽

Fungal Species ◽

Plant Performance ◽

Plant Growth Promoting Bacteria ◽

Microbial Species ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Dual Inoculation

In natural and agricultural ecosystems, survival and growth of plants depend substantially on microbes in the endosphere and rhizosphere. Although numerous studies have reported the presence of plant-growth promoting bacteria and fungi in below-ground biomes, it remains a major challenge to understand how sets of microbial species positively or negatively affect plants' performance. By conducting a series of single- and dual-inoculation experiments of 13 endophytic and soil fungi targeting a Brassicaceae plant species, we here evaluated how microbial effects on plants depend on presence/absence of co-occurring microbes. The comparison of single- and dual-inoculation experiments showed that combinations of the fungal isolates with the highest plant-growth promoting effects in single inoculations did not yield highly positive impacts on plant performance traits (e.g., shoot dry weight). In contrast, pairs of fungi including small/moderate contributions to plants in single-inoculation contexts showed the greatest effects on plants among the 78 fungal pairs examined. These results on the offset and synergistic effects of pairs of microbes suggest that inoculation experiments of single microbial species/isolates can result in the overestimation or underestimation of microbial functions in multi-species contexts. Because keeping single-microbe systems in outdoor conditions is impractical, designing sets of microbes that can maximize performance of crop plants is an important step for the use of microbial functions in sustainable agriculture.

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Micro-remediation of chromium contaminated soils

PeerJ ◽

10.7717/peerj.6076 ◽

2018 ◽

Vol 6 ◽

pp. e6076 ◽

Cited By ~ 7

Author(s):

Hadia -e- Fatima ◽

Ambreen Ahmed

Keyword(s):

Plant Growth ◽

Contaminated Soils ◽

Crop Production ◽

Lens Culinaris ◽

Production Plant ◽

Plant Growth Promoting Bacteria ◽

Bacterial Strains ◽

Soil Release ◽

Plant Growth Promoting ◽

Growth Promoting

Bacteria are tiny organisms which are ubiquitously found in the environment. These microscopic living bodies are responsible for the flow of nutrients in biogeochemical cycles and fertility imparted to the soil. Release of excessive chromium in agricultural soils due to rapid growth of industries may result in minimizing the fertility of soil in future, which will lead to reduction in crop production. Plant growth promoting bacteria (PGPB) are beneficial to the environment, some of which can tolerate chromium and protect plants against heavy metal stress. The current study aims to identify such chromium-tolerant auxin-producing rhizobacteria and to investigate their inoculation effects on the growth characteristics of Lens culinaris in chromium polluted soils by using two different chromium salts i.e., K2Cr2O7 and K2CrO4 in varying concentrations (0, 50, 100, 200, 400 and 500 µgml−1). The results revealed that Bacillus species are efficient in significantly reducing the deleterious effects of Cr. These effective bacterial strains were able to stimulate the growth of metal effected plants of Lens culinaris which were grown in chromium contaminated environment. Therefore, these plant growth promoting rhizobacteria PGPRs, having both auxin production potential and chromium-resistance ability, are considered as efficient micro-factories against chromium pollution.

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Effect of plant growth promoting bacteria on early growth of wheat cultivars

Journal of Applied Biological Chemistry ◽

10.3839/jabc.2019.033 ◽

2019 ◽

Vol 62 (3) ◽

pp. 247-250

Author(s):

Sang Gyu Lee ◽

Hyeri Lee ◽

Jimin Lee ◽

Byung Cheon Lee ◽

Hojoung Lee ◽

...

Keyword(s):

Plant Growth ◽

Early Growth ◽

Plant Growth Promoting Bacteria ◽

Wheat Cultivars ◽

Plant Growth Promoting ◽

Growth Promoting

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Plant growth promotion of barley (Hordeum vulgare L.) by potassium solubilizing bacteria with multifarious plant growth promoting attributes

Plant Science Today ◽

10.14719/pst.1377 ◽

2021 ◽

Vol 8 (sp1) ◽

pp. 17-24

Author(s):

Tanvir Kaur ◽

Rubee Devi ◽

Divjot Kour ◽

Ashok Yadav ◽

Ajar Nath Yadav

Keyword(s):

Plant Growth ◽

Environmental Sustainability ◽

Growth Promotion ◽

Growth Parameters ◽

Sugar Content ◽

Plant Growth Promoting Bacteria ◽

Bacterial Strains ◽

Hordeum Vulgare L ◽

Plant Growth Promoting ◽

Growth Promoting

Potassium (K) is the foremost macronutrients for growth of plant, soil health and fertility. The huge application of NPK chemical fertilizers negatively impacts the economy and is a threat to environmental sustainability. The rapid depletion of K mineral in soil is due to the application of agrochemicals agricultural fields for the production of crops in India. In present investigation, K-solubilizing microbes (KSM) were isolated and enumerated from cereal crops growing in Sirmour Himachal Pradesh. A total 125 bacteria were isolated and screened for K- solubilization on Aleksandrov agar plates and found that 31 bacterial strains exhibited K-solubilization. These 31 K-solubilizing strains of bacteria were additionally screened for other plant growth promoting (PGP) potential including solubilization of minerals, production of siderophores, ammonia, hydrogen cyanide and indole acetic acids. The performance of an efficient K-solubilizer was evaluated for plant growth promoting ability in pot assay under in vitro conditions. The strain EU-LWNA-25 positively influenced shoot length, fresh weight, carotenoids and total sugar content than the full dose, half dose and control. The strain enhancing physiological and growth parameters was identified by BLASTn analysis as Pseudomonas gessardii EU-LWNA-25. K-solubilizing plant growth promoting bacteria could be suitable bioinoculants for Rabi seasonal crops and overcomes the challenges of sustainable agriculture in K-deficient soil.

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Interaction between sugarcane families and plant growth-promoting bacteria in two crop cycles

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n2p527 ◽

2019 ◽

Vol 40 (2) ◽

pp. 527 ◽

Cited By ~ 2

Author(s):

Valéria Rosa Lopes ◽

João Carlos Bespalhok Filho ◽

Guilherme Grodzki Oliveira Figueiredo ◽

Ricardo Augusto de Oliveira ◽

Edelclaiton Daros

Keyword(s):

Plant Growth ◽

Average Height ◽

Plant Growth Promoting Bacteria ◽

Plant Genotype ◽

Saccharum Spp ◽

Stalk Diameter ◽

Plant Growth Promoting ◽

Azospirillum Amazonense ◽

Growth Promoting ◽

Selection Of

The response of sugarcane (Saccharum spp.) families to the inoculation with plant growth-promoting bacteria (PGPB) may show positive or negative interaction values depending on the plant genotype, allowing the selection of responsive genotypes to the association. Based on these precepts, this study aimed to evaluate the response of 27 sugarcane families treated with two PGPB-based inoculants in two sugarcane cycles: plant-cane and first ratoon. Four months after germination, 27 families from crosses between clones of sugarcane were: (T0) not inoculated; (T1) inoculated with Triazo, which consists of a mixture of the strains Abv5, Abv6, and Abv7 of Azospirillum brasilense; and (T2) inoculated with an inoculant called Mix, which contains five strains of PGPB (Gluconacetobacter diazotrophicus Pal5, Azospirillum amazonense CBAmC, Burkholderia tropica Ppe8, Herbaspirillum rubrisubalbicans HCc103, and Herbaspirillum seropedicae HRC54). The productivity traits evaluated were the number of stalks, average height, stalk diameter, and Brix. The correlation values between the two cycles were 0.87 for the number of stalks, 0.70 for average height, 0.48 for stalk diameter, and 0.63 for Brix. Differences were observed between treatments for average height, stalk diameter, and Brix. Differences were also observed between families for all the studied traits. Brix was the only parameter that presented a significant interaction between family and inoculant. Different responses were observed between cycles for some families, showing that other factors can also influence the association.

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Azospirillum plant growth-promoting strains are nonpathogenic on tomato, pepper, cotton, and wheat

Canadian Journal of Microbiology ◽

10.1139/w97-136 ◽

1998 ◽

Vol 44 (2) ◽

pp. 168-174 ◽

Cited By ~ 20

Author(s):

Yoav Bashan

Keyword(s):

Plant Growth ◽

Plant Species ◽

Pseudomonas Syringae ◽

Xanthomonas Campestris ◽

Plant Pathogens ◽

Dry Weight ◽

Plant Growth Promoting Bacteria ◽

Disease Symptoms ◽

Plant Growth Promoting ◽

Growth Promoting

Six strains of Azospirillum belonging to five species of plant growth-promoting bacteria (A. brasilense, A. lipoferum, A. amazonense, A. irakense, and A. halopraeference) did not cause visible disease symptoms on the roots or leaves of tomato, pepper, cotton, and wheat, failed to inhibit seed germination, and did not reduce plant dry weight when seven standard techniques for the inoculation of plant pathogens were used. Similar inoculation conditions with plant pathogens (Pseudomonas syringae pv. tomato, Xanthomonas campestris pv. vesicatoria, Xanthomonas campestris pv. translucens, and Xanthomonas campestris pv. malvacearum) induced typical disease symptoms. None of Azospirillum strains caused the hypersensitive reaction on eggplant, whereas all pathogens did. All Azospirillum strains increased phytoalexin production in all disease-resistant plant species to moderate levels, but the levels were significantly lower than those induced by the compatible pathogens. The various phytoalexins produced in plants had the capacity to inhibit growth of all Azospirillum strains. Azospirillum amazonense, A. irakense, and A. halopraeference had no apparent effect on plant growth, while A. brasilense and A. lipoferum increased the dry weight of all plant species. Under partial mist conditions, all Azospirillum strains were capable of colonizing leaf surfaces (103-107 cfu/g dry weight) regardless of the plant species. These results provide experimental evidence that Azospirillum sp. might be considered safe for the inoculation of several plant species.Key words: Azospirillum, beneficial bacteria, environmental protection, plant inoculation, plant growth-promoting bacteria.

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