scholarly journals Tomato leaf curl New Delhi virus: a widespread bipartite begomovirus in the territory of monopartite begomoviruses

2016 ◽  
Vol 18 (7) ◽  
pp. 901-911 ◽  
Author(s):  
Syed Shan-E-Ali Zaidi ◽  
Darren P. Martin ◽  
Imran Amin ◽  
Muhammad Farooq ◽  
Shahid Mansoor
Keyword(s):  
Monopartite Begomoviruses ◽  
Tomato Leaf ◽  
New Delhi ◽  
Bipartite Begomovirus ◽  
Tomato Leaf Curl ◽  
Leaf Curl

scholarly journals Frequent occurrence of Mungbean yellow mosaic India virus in tomato leaf curl disease affected tomato in Oman

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
M. S. Shahid ◽  
M. Shafiq ◽  
M. Ilyas ◽  
A. Raza ◽  
M. N. Al-Sadrani ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Monopartite Begomoviruses ◽  
Tomato Leaf ◽  
Bipartite Begomovirus ◽  
Rolling Circle ◽  
Tomato Leaf Curl Disease ◽  
Planting Material ◽  
Next Generation Sequencing Ngs ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Abstract Next generation sequencing (NGS) of DNAs amplified by rolling circle amplification from 6 tomato (Solanum lycopersicum) plants with leaf curl symptoms identified a number of monopartite begomoviruses, including Tomato yellow leaf curl virus (TYLCV), and a betasatellite (Tomato leaf curl betasatellite [ToLCB]). Both TYLCV and ToLCB have previously been identified infecting tomato in Oman. Surprisingly the NGS results also suggested the presence of the bipartite, legume-adapted begomovirus Mungbean yellow mosaic Indian virus (MYMIV). The presence of MYMIV was confirmed by cloning and Sanger sequencing from four of the six plants. A wider analysis by PCR showed MYMIV infection of tomato in Oman to be widespread. Inoculation of plants with full-length clones showed the host range of MYMIV not to extend to Nicotiana benthamiana or tomato. Inoculation to N. benthamiana showed TYLCV to be capable of maintaining MYMIV in both the presence and absence of the betasatellite. In tomato MYMIV was only maintained by TYLCV in the presence of the betasatellite and then only at low titre and efficiency. This is the first identification of TYLCV with ToLCB and the legume adapted bipartite begomovirus MYMIV co-infecting tomato. This finding has far reaching implications. TYLCV has spread around the World from its origins in the Mediterranean/Middle East, in some instances, in live tomato planting material. The results here may suggest that begomoviruses which do not commonly infect tomato, such as MYMIV, could be spread as a passenger of TYLCV in tomato.

scholarly journals Seed Transmission of Tomato Leaf Curl New Delhi Virus from Zucchini Squash in Italy

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 563 ◽  
Author(s):  
Eui-Joon Kil ◽  
Thuy Thi Bich Vo ◽  
Chairina Fadhila ◽  
Phuong Thi Ho ◽  
Aamir Lal ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Indian Subcontinent ◽  
Tomato Leaf ◽  
Economic Damage ◽  
Mechanical Transmission ◽  
New Delhi ◽  
Bipartite Begomovirus ◽  
Insect Vector ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus affecting tomato cultivation on the Indian subcontinent. Recently, however, a new strain of the virus, named ToLCNDV-ES, has spread to Mediterranean countries such as Spain, Italy, and Tunisia, and occurred in Cucurbita crops, causing economic damage. Although ToLCNDV is spread by the sweet potato whitefly (Bemisia tabaci), like other begomoviruses, it has not been clear how ToLCNDV suddenly spread from the Indian subcontinent to the Mediterranean region. In 2017, ToLCNDV was diagnosed in young seedlings germinated naturally from fruits fallen in the prior year on a farm located in Giugliano in Campania, Naples, Italy, suggesting a possible role of the seeds in vertical transmission of the virus. Because sweet potato whiteflies were widespread naturally in that region, it was necessary to verify that in an artificial insect vector-free condition. Seeds were harvested from two ToLCNDV-infected zucchini squash cultivars in Naples in 2017 and 2018 to examine whether ToLCNDV can be transmitted from zucchini squash seeds to young plants. Viral DNA was amplified from these seeds and 1- to 3-week-old seedlings germinated from them with a ToLCNDV-specific primer set. According to PCR results, viral contamination was confirmed from all harvested seeds and dissemination was proven from 61.36% of tested seedling samples. Mechanical transmission from seed-borne virus-infected seedlings to healthy zucchini squash plants was also succesful, demonstrating that seedlings from ToLCNDV-infected seeds did act as inoculum. This is the first report demonstrating that ToLCNDV is a seed-transmissible virus in zucchini squash plants in Italy.

scholarly journals First Detection of Tomato leaf curl New Delhi virus Infecting Zucchini in Spain

Plant Disease ◽  
2014 ◽  
Vol 98 (6) ◽  
pp. 857-857 ◽  
Author(s):  
M. Juárez ◽  
R. Tovar ◽  
E. Fiallo-Olivé ◽  
M. A. Aranda ◽  
B. Gosálvez ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Bipartite Begomoviruses ◽  
Nucleotide Identity ◽  
New Delhi ◽  
Bipartite Begomovirus ◽  
Species Demarcation ◽  
Begomovirus Infection ◽  
Two Samples ◽  
Tomato Leaf Curl ◽  
Leaf Curl

In September 2012, a novel disease syndrome was observed in zucchini (Cucurbita pepo L.) crops in Murcia Province (southeastern Spain). Symptoms included curling, vein swelling, and severe mosaic in young leaves, short internodes, and fruit skin roughness, resembling begomovirus infection. Similar symptoms were observed in May 2013 in Almería Province (southern Spain). DNA was isolated from 8 and 7 symptomatic leaf samples collected in Murcia and Almería, respectively, and analyzed by PCR with primers GemCP-V-5′ and GemCP-C-3′ designed to detect begomoviruses by amplifying the core of coat protein gene (CP) (3). DNA fragments of the expected size (~600 bp) were amplified supporting a begomovirus infection. The DNA sequences obtained from four samples were identical. BLAST analysis showed the highest nucleotide identity (98%) with partial CP gene sequences from isolates of Tomato leaf curl New Delhi virus (ToLCNDV) infecting cucumber in India (GenBank Accession No. KC846817). ToLCNDV, a bipartite begomovirus first reported from tomato, also infects other solanaceous and cucurbitaceous crops in India and neighboring countries (1). DNA from two samples from Murcia and three from Almería was used for rolling-circle amplification using ϕ29 DNA polymerase (TempliPhi kit, GE Healthcare, Little Chalfont, UK) and digested with a set of restriction endonucleases. All five samples yielded amplification products with identical restriction patterns. Two samples from Murcia (MU-8.1 and MU-11.1) and one from Almería (AL-661) were selected to clone the putative DNA-A and DNA-B begomovirus genome components by using single BamHI or NcoI sites. Inserts of two clones from each sample, one corresponding to DNA-A and one to DNA-B, were completely sequenced. The cloned genomes exhibited the typical organization of Old World bipartite begomoviruses (1). Sequences were aligned with begomovirus sequences available in databases using MUSCLE and pairwise identity scores were calculated with SDT (species demarcation tool [4]). DNA-A sequences obtained from Murcia (2,738 nt, KF749224 and KF749225) and Almería (2,738 nt, KF749223) shared >99% nucleotide identity, with the highest nucleotide identity (91.3 to 91.5%) with that of an Indian ToLCNDV isolate from chilli (HM007120). DNA-B sequences (2,684 nt, KF749226, KF749227, and KF749228) shared >99% nucleotide identity, and showed the highest nucleotide identity (83.1 to 83.3%) with that of a Pakistani ToLCNDV isolate from Solanum nigrum (AJ620188). Nucleotide sequence identity of DNA-A with the most closely related begomoviruses was above the 91% threshold for species demarcation (2), thus confirming that the begomoviruses found infecting zucchini in Spain are isolates of ToLCNDV. In fall 2013, the disease was widespread in zucchini both in Murcia and Almería, and ToLCNDV has also been found infecting melon and cucumber crops. To our knowledge, this is the first report of a bipartite begomovirus in Spain and Europe. References: (1) J. K. Brown et al. Page 351 in: Virus Taxonomy. Ninth Report of the ICTV. A. M. Q. King et al., eds. Elsevier/Academic Press, London, 2012. (2) ICTV Geminiviridae Study Group. New species and revised taxonomy proposal for the genus Begomovirus (Geminiviridae). ICTV. Retrieved from http://talk.ictvonline.org/files/proposals/ taxonomy_proposals_plant1/m/plant04/4720.aspx , 10 October 2013. (3) H. Lecoq and C. Desbiez. Adv. Virus Res. 84:67, 2012. (4) B. Muhire et al. Arch. Virol. 158:1411, 2013.

scholarly journals First Report of Tomato leaf curl New Delhi virus, a Bipartite Begomovirus, Infecting Soybean (Glycine max)

Plant Disease ◽  
2017 ◽  
Vol 101 (5) ◽  
pp. 845-845 ◽  
Author(s):  
N. Jamil ◽  
A. Rehman ◽  
M. Hamza ◽  
A. Hafeez ◽  
H. Ismail ◽  
...  
Keyword(s):  
Glycine Max ◽  
Tomato Leaf ◽  
New Delhi ◽  
Bipartite Begomovirus ◽  
First Report ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Screening Luffa germplasm and advanced breeding lines for resistance to Tomato leaf curl New Delhi virus

2021 ◽  
Author(s):  
Manpreet Kaur ◽  
B. Varalakshmi ◽  
M. Pitchaimuthu ◽  
B. Mahesha
Keyword(s):  
Tomato Leaf ◽  
New Delhi ◽  
Breeding Lines ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Potato apical leaf curl disease: current status and perspectives on a disease caused by tomato leaf curl New Delhi virus

2021 ◽  
Author(s):  
Ravinder Kumar ◽  
Rahul Kumar Tiwari ◽  
Arjunan Jeevalatha ◽  
Sundaresha Siddappa ◽  
Mohd. Abas Shah ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Current Status ◽  
New Delhi ◽  
Apical Leaf ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Inhibition of Bemisia tabaci vectored, GroEL mediated transmission of tomato leaf curl New Delhi virus by garlic leaf lectin (Allium sativum leaf agglutinin)

2021 ◽  
pp. 198443
Author(s):  
Ayan Das ◽  
Amit Roy ◽  
Arunava Mandal ◽  
Hossian Ali Mondal ◽  
Daniel Hess ◽  
...  
Keyword(s):  
Bemisia Tabaci ◽  
Allium Sativum ◽  
Tomato Leaf ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Tomato leaf curl New Delhi virus. [Distribution map].

2016 ◽  
Author(s):  
Keyword(s):  
Tamil Nadu ◽  
Uttar Pradesh ◽  
Tomato Leaf ◽  
New Delhi ◽  
Distribution Map ◽  
Tomato Leaf Curl ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe ◽  
Leaf Curl

Abstract A new distribution map is provided for Tomato leaf curl New Delhi virus. Geminiviridae: Begomovirus. Hosts: tomato (Solanum lycopersicum) and other Solanaceae such as aubergine (S. melongena), potato (S. tuberosum), Capsicum spp. and Cucurbitaceae. Information is given on the geographical distribution in Europe (Italy, Sicily, Spain, Mainland Spain), Asia (Bangladesh, India, Andhra Pradesh, Delhi, Gujarat, Haryana, Karnataka, Maharashtra, Punjab, Tamil Nadu, Uttar Pradesh, West Bengal, Indonesia, Java, Iran, Pakistan, Philippines, Sri Lanka, Taiwan and Thailand) and Africa (Tunisia).

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