scholarly journals Large-scale molecular genetic analysis in plant-pathogenic fungi: a decade of genome-wide functional analysis

2017 ◽  
Vol 18 (5) ◽  
pp. 754-764 ◽  
Author(s):  
Thabiso E. Motaung ◽  
Hiromasa Saitoh ◽  
Toi J. Tsilo
Keyword(s):  
Functional Analysis ◽  
Genetic Analysis ◽  
Large Scale ◽  
Molecular Genetic ◽  
Pathogenic Fungi ◽  
Molecular Genetic Analysis ◽  
Plant Pathogenic Fungi ◽  
Genome Wide

scholarly journals Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3416
Author(s):  
Andrey Rodionov ◽  
Tatiana Deniskova ◽  
Arsen Dotsev ◽  
Valeria Volkova ◽  
Sergey Petrov ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
National Park ◽  
Molecular Genetic ◽  
Dna Typing ◽  
Molecular Genetic Analysis ◽  
Snp Genotyping ◽  
Wildlife Crime ◽  
Wildlife Forensics ◽  
Genome Wide

Poaching is one of the major types of wildlife crime in Russia. Remnants of goats (presumably the wild endemic species, the Caucasian tur) were found in an area of the Caucasian mountains. The case study involves a suspected poacher whose vehicle was found to have two duffel bags containing pieces of a carcass, which he claimed was that of a goat from his flock. The aim of the forensic genetic analysis for this case was to (i) establish individual identity and (ii) perform species identification. DNA typing based on fourteen microsatellites revealed that STR-genotypes generated from pieces of evidence found at crime scene fully matched those obtained from the evidence seized from the suspect. The results of genome-wide SNP-genotyping, using Illumina Goat SNP50 BeadChip, provided evidence that the poached animal was a wild Caucasian tur (Capra caucasica). Thus, based on comprehensive molecular genetic analysis, evidence of poaching was obtained and sent to local authorities. To our knowledge, this case study is the first to attempt to use DNA chips in wildlife forensics of ungulates.

scholarly journals Domestic devices for molecular genetic analysis: developments of the IAP RAS and SINTOL LLC

2021 ◽  
Vol 40 (3) ◽  
pp. 69-74
Author(s):  
Vladimir E. Kurochkin ◽  
Yakov I. Alekseev ◽  
Dmitriy G. Petrov ◽  
Anatoliy A. Evstrapov
Keyword(s):  
Genetic Analysis ◽  
Nucleic Acids ◽  
Dna Sequencing ◽  
Real Time ◽  
High Efficiency ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Specific Indication ◽  
Genome Wide ◽  
Wide Range

The set of domestic devices and reagent kits for molecular genetic analysis has been developed, which provides for all stages of the study, from the automated isolation of nucleic acids, their amplification with real-time detection to classical and genome-wide DNA sequencing. With the help of the created equipment and reagent kits, approaches for the specific indication of pathogens of a wide range of diseases have been successfully implemented. The high efficiency of the proposed approaches is shown by the example of detecting and analyzing the RNA of the pathogen SARS-CoV-2 in the fight against the COVID-19 coronavirus pandemic (7 figs, bibliography: 4 refs).

scholarly journals Molecular Genetic Analysis of RB1 gene among Sudanese children with Retinoblastoma

2019 ◽  
Author(s):  
Nada O. Ibrahim ◽  
Mahgoub Saleem ◽  
Entesar Eltayeb ◽  
Salwa Mekki ◽  
Elteleb G. Elnaim ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Genetic Analysis ◽  
In Silico ◽  
Molecular Genetic ◽  
Geographical Area ◽  
Good Prognosis ◽  
Molecular Genetic Analysis ◽  
Molecular Genetic Study ◽  
Genetic Sequencing ◽  
Life Threatening

BACKGROUNDRetinoblastoma (RB), the commonest early childhood intraocular tumor, is most often related to mutations in the RB1 gene with an incidence of 3% of all pediatric tumors. It has good prognosis if diagnosed early but it is life-threatening when diagnosed late.OBJECTIVETo study the Molecular Genetic Analysis of Retinoblastoma (RB) in Sudanese families.METHODSThirty one (n=31) clinically and histopathologically diagnosed cases of RB attending Makkah Eye Complex (MEC) Orbit clinic (Khartoum, Sudan) were included in this Molecular Genetic RB Analysis. Fresh blood samples extracted from seven RB patients and 15 close families for DNA extraction and PCR were sent for Genetic Sequencing and In silico approach for “Exon 18 mutations” which is one of the highly mutated exons worldwide.RESULTSThe majority of patients (41.9%) were below five years old. Females were 58.1%, males were 41.9%. Leukocoria was the commonest sign at presentation (41.9%). RB Unilaterality were in (77.4%) while Bilaterality in 19.4%. Both eyes were equally affected 50% each. The age at diagnosis time ranged from 0.02 to five years. Consanguinity of parents was very high (85.7%), the 1st degree cousins were less (28.6%) while the 2nd degree was high (57.1%). The patients’ ethnic background and geographical area were from seven different tribes; all belong to the Western Sudan. The molecular genetic study showed that exon 18 was free of mutation among the seven patients + their three relatives. The Functional Analysis and (SNPs) prediction study of exon 18 from NCBI data base showed that the various computational approaches used (SIFT, PolyPhen-2, I-mutant and Project hope) identified 16 reported mutations worldwide, three of which (rs137853292, rs375645171 and rs772068738) are major nsSNPs (non-synonymous) which might contribute to native RB1 protein malfunction and ultimately causing carcinoma.CONCLUSIONRB mainly affected children under five years and both sexes are equally affected. Unilaterality was predominant. Consanguinity plays a role in inheritance and the majority of patients were from Western Sudan. The most commonly detected deleterious mutations worldwide in exon 18 were not found in the Sudanese studies samples. Further screening for the highly reported mutations in exons 8, 10 and 14 or Next Generation Sequencing (NGS) are recommended. In silico tools are useful in studying the functional analysis of SNPs.

scholarly journals Repeated molecular genetic analysis in Brugada syndrome revealed a novel disease-associated large deletion in the SCN5A gene

2016 ◽  
Vol 2 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Anders Krogh Broendberg ◽  
Lisbeth Noerum Pedersen ◽  
Jens Cosedis Nielsen ◽  
Henrik Kjaerulf Jensen
Keyword(s):  
Genetic Analysis ◽  
Brugada Syndrome ◽  
Molecular Genetic ◽  
Large Deletion ◽  
Molecular Genetic Analysis ◽  
Scn5a Gene

scholarly journals Functional analysis of the conserved transcriptional regulator CfWor1 inCladosporium fulvumreveals diverse roles in the virulence of plant pathogenic fungi

2014 ◽  
Vol 92 (1) ◽  
pp. 10-27 ◽  
Author(s):  
Bilal Ökmen ◽  
Jérôme Collemare ◽  
Scott Griffiths ◽  
Ate van der Burgt ◽  
Russell Cox ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Transcriptional Regulator ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi
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