scholarly journals Domestic devices for molecular genetic analysis: developments of the IAP RAS and SINTOL LLC

2021 ◽  
Vol 40 (3) ◽  
pp. 69-74
Author(s):  
Vladimir E. Kurochkin ◽  
Yakov I. Alekseev ◽  
Dmitriy G. Petrov ◽  
Anatoliy A. Evstrapov
Keyword(s):  
Genetic Analysis ◽  
Nucleic Acids ◽  
Dna Sequencing ◽  
Real Time ◽  
High Efficiency ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Specific Indication ◽  
Genome Wide ◽  
Wide Range

The set of domestic devices and reagent kits for molecular genetic analysis has been developed, which provides for all stages of the study, from the automated isolation of nucleic acids, their amplification with real-time detection to classical and genome-wide DNA sequencing. With the help of the created equipment and reagent kits, approaches for the specific indication of pathogens of a wide range of diseases have been successfully implemented. The high efficiency of the proposed approaches is shown by the example of detecting and analyzing the RNA of the pathogen SARS-CoV-2 in the fight against the COVID-19 coronavirus pandemic (7 figs, bibliography: 4 refs).

scholarly journals Combination of Multiple Microsatellite Analysis and Genome-Wide SNP Genotyping Helps to Solve Wildlife Crime: A Case Study of Poaching of a Caucasian tur (Capra caucasica) in Russian Mountain National Park

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3416
Author(s):  
Andrey Rodionov ◽  
Tatiana Deniskova ◽  
Arsen Dotsev ◽  
Valeria Volkova ◽  
Sergey Petrov ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
National Park ◽  
Molecular Genetic ◽  
Dna Typing ◽  
Molecular Genetic Analysis ◽  
Snp Genotyping ◽  
Wildlife Crime ◽  
Wildlife Forensics ◽  
Genome Wide

Poaching is one of the major types of wildlife crime in Russia. Remnants of goats (presumably the wild endemic species, the Caucasian tur) were found in an area of the Caucasian mountains. The case study involves a suspected poacher whose vehicle was found to have two duffel bags containing pieces of a carcass, which he claimed was that of a goat from his flock. The aim of the forensic genetic analysis for this case was to (i) establish individual identity and (ii) perform species identification. DNA typing based on fourteen microsatellites revealed that STR-genotypes generated from pieces of evidence found at crime scene fully matched those obtained from the evidence seized from the suspect. The results of genome-wide SNP-genotyping, using Illumina Goat SNP50 BeadChip, provided evidence that the poached animal was a wild Caucasian tur (Capra caucasica). Thus, based on comprehensive molecular genetic analysis, evidence of poaching was obtained and sent to local authorities. To our knowledge, this case study is the first to attempt to use DNA chips in wildlife forensics of ungulates.

scholarly journals Repeated molecular genetic analysis in Brugada syndrome revealed a novel disease-associated large deletion in the SCN5A gene

2016 ◽  
Vol 2 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Anders Krogh Broendberg ◽  
Lisbeth Noerum Pedersen ◽  
Jens Cosedis Nielsen ◽  
Henrik Kjaerulf Jensen
Keyword(s):  
Genetic Analysis ◽  
Brugada Syndrome ◽  
Molecular Genetic ◽  
Large Deletion ◽  
Molecular Genetic Analysis ◽  
Scn5a Gene

scholarly journals Molecular genetic analysis of a class B periplasmic-flagellum gene of Treponema phagedenis.

1992 ◽  
Vol 174 (20) ◽  
pp. 6404-6410 ◽  
Author(s):  
R J Limberger ◽  
L L Slivienski ◽  
D B Yelton ◽  
N W Charon
Keyword(s):  
Genetic Analysis ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Class B ◽  
Flagellum Gene
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