scholarly journals The cis ‐expression of the coat protein of turnip mosaic virus is essential for viral intercellular movement in plants

2020 ◽  
Vol 21 (9) ◽  
pp. 1194-1211 ◽  
Author(s):  
Zhaoji Dai ◽  
Rongrong He ◽  
Mark A. Bernards ◽  
Aiming Wang
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Intercellular Movement

scholarly journals Contribution of Host Intracellular Transport Machineries to Intercellular Movement of Turnip Mosaic Virus

2013 ◽  
Vol 9 (10) ◽  
pp. e1003683 ◽  
Author(s):  
Maxime Agbeci ◽  
Romain Grangeon ◽  
Richard S. Nelson ◽  
Huanquan Zheng ◽  
Jean-François Laliberté
Keyword(s):  
Mosaic Virus ◽  
Intracellular Transport ◽  
Turnip Mosaic Virus ◽  
Intercellular Movement

scholarly journals Conversion in the Requirement of Coat Protein in Cell-to-Cell Movement Mediated by the Cucumber Mosaic Virus Movement Protein

2001 ◽  
Vol 75 (17) ◽  
pp. 8045-8053 ◽  
Author(s):  
Hideaki Nagano ◽  
Kazuyuki Mise ◽  
Iwao Furusawa ◽  
Tetsuro Okuno
Keyword(s):  
Amino Acids ◽  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Cell Movement ◽  
Plant Viruses ◽  
Brome Mosaic Virus ◽  
Viral Movement ◽  
Intercellular Movement

ABSTRACT Plant viruses have movement protein (MP) gene(s) essential for cell-to-cell movement in hosts. Cucumber mosaic virus (CMV) requires its own coat protein (CP) in addition to the MP for intercellular movement. Our present results using variants of both CMV and a chimeric Brome mosaic virus with the CMV MP gene revealed that CMV MP truncated in its C-terminal 33 amino acids has the ability to mediate viral movement independently of CP. Coexpression of the intact and truncated CMV MPs extremely reduced movement of the chimeric viruses, suggesting that these heterogeneous CMV MPs function antagonistically. Sequential deletion analyses of the CMV MP revealed that the dispensability of CP occurred when the C-terminal deletion ranged between 31 and 36 amino acids and that shorter deletion impaired the ability of the MP to promote viral movement. This is the first report that a region of MP determines the requirement of CP in cell-to-cell movement of a plant virus.

scholarly journals Nucleotide Sequences of the Coat Protein Genes of Aphid Transmissible and Non-Transmissible Isolates of Turnip Mosaic Virus.

1991 ◽  
Vol 57 (4) ◽  
pp. 549-557 ◽  
Author(s):  
Hiroyuki NAKASHIMA ◽  
Nobumichi SAKO ◽  
Keiichiro JOH ◽  
Katsuji HORI ◽  
Fukuji NONAKA
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Nucleotide Sequences

scholarly journals Molecular cloning and nucleotide sequence of coat protein gene of turnip mosaic virus

1990 ◽  
Vol 18 (18) ◽  
pp. 5555-5555 ◽  
Author(s):  
Ling-Jie Kong ◽  
Rong-Xiang Fang ◽  
Zheng-Hua Chen ◽  
Ke-Qiang Mang
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Molecular Cloning ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Turnip Mosaic Virus

scholarly journals RNA virus interference via CRISPR/Cas13a system in plants

2017 ◽  
Author(s):  
Rashid Aman ◽  
Zahir Ali ◽  
Haroon Butt ◽  
Ahmed Mahas ◽  
Fatima Aljedaani ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Fluorescent Protein ◽  
Phage Genome ◽  
Rna Viruses ◽  
Rna Virus ◽  
Turnip Mosaic Virus ◽  
Green Fluorescent

AbstractCRISPR/Cas systems confer immunity against invading nucleic acids and phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 type VI-A ribonuclease capable of targeting and cleaving single stranded RNA (ssRNA) molecules of the phage genome. Here, we employ CRISPR/Cas13a to engineer interference with an RNA virus, Turnip Mosaic Virus (TuMV), in plants. CRISPR/Cas13a produced interference against green fluorescent protein (GFP) expressing TuMV in transient assays and stable overexpression lines of Nicotiana benthamiana. crRNAs targeting the HC-Pro and GFP sequences exhibited better interference than those targeting other regions such as coat protein (CP) sequence. Cas13a can also process pre-crRNAs into functional crRNAs. Our data indicate that CRISPR/Cas13a can be used for engineering interference against RNA viruses, providing a potential novel mechanism for RNA-guided immunity against RNA viruses, and for other RNA manipulations in plants.

Remorin interacting with PCaP1 impairs Turnip mosaic virus intercellular movement but is antagonised by VPg

2019 ◽  
Vol 225 (5) ◽  
pp. 2122-2139 ◽  
Author(s):  
Guangyuan Cheng ◽  
Zongtao Yang ◽  
Hai Zhang ◽  
Jisen Zhang ◽  
Jingsheng Xu
Keyword(s):  
Mosaic Virus ◽  
Turnip Mosaic Virus ◽  
Intercellular Movement

scholarly journals Structure of Turnip mosaic virus and its viral-like particles

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Rebeca Cuesta ◽  
Carmen Yuste-Calvo ◽  
David Gil-Cartón ◽  
Flora Sánchez ◽  
Fernando Ponz ◽  
...  
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Plant Virus ◽  
Plant Viruses ◽  
Turnip Mosaic Virus ◽  
Correct Orientation ◽  
Bona Fide ◽  
Rna Interaction ◽  
Genus One

Abstract Turnip mosaic virus (TuMV), a potyvirus, is a flexible filamentous plant virus that displays a helical arrangement of coat protein copies (CPs) bound to the ssRNA genome. TuMV is a bona fide representative of the Potyvirus genus, one of most abundant groups of plant viruses, which displays a very wide host range. We have studied by cryoEM the structure of TuMV virions and its viral-like particles (VLPs) to explore the role of the interactions between proteins and RNA in the assembly of the virions. The results show that the CP-RNA interaction is needed for the correct orientation of the CP N-terminal arm, a region that plays as a molecular staple between CP subunits in the fully assembled virion.

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