scholarly journals Effect of trapping method on species identification of phlebotomine sandflies by MALDI-TOF MS protein profiling

2018 ◽  
Vol 32 (3) ◽  
pp. 388-392 ◽  
Author(s):  
P. Halada ◽  
K. Hlavackova ◽  
J. Risueño ◽  
E. Berriatua ◽  
P. Volf ◽  
...  
Keyword(s):  
Species Identification ◽  
Protein Profiling ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Phlebotomine Sandflies ◽  
Trapping Method ◽  
Tof Ms

scholarly journals An integrative approach to identify sand fly vectors of leishmaniasis in Ethiopia by morphological and molecular techniques

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Myrthe Pareyn ◽  
Vit Dvorak ◽  
Petr Halada ◽  
Natalie Van Houtte ◽  
Nigatu Girma ◽  
...  
Keyword(s):  
Species Identification ◽  
Molecular Techniques ◽  
Protein Profiling ◽  
Sand Fly ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Human Leishmaniasis ◽  
Sand Fly Vectors ◽  
Morphological Assessment ◽  
Tof Ms

Abstract Background Ethiopia is affected by human leishmaniasis caused by several Leishmania species and transmitted by a variety of sand fly vectors of the genus Phlebotomus. The sand fly fauna in Ethiopia is highly diverse and some species are closely related and similar in morphology, resulting in difficulties with species identification that requires deployment of molecular techniques. DNA barcoding entails high costs, requires time and lacks reference sequences for many Ethiopian species. Yet, proper species identification is pivotal for epidemiological surveillance as species differ in their actual involvement in transmission cycles. Recently, protein profiling using MALDI-TOF mass spectrometry has been introduced as a promising technique for sand fly identification. Methods In our study, we used an integrative taxonomic approach to identify most of the important sand fly vectors of leishmaniasis in Ethiopia, applying three complementary methods: morphological assessment, sequencing analysis of two genetic markers, and MALDI-TOF MS protein profiling. Results Although morphological assessment resulted in some inconclusive identifications, both DNA- and protein-based techniques performed well, providing a similar hierarchical clustering pattern for the analyzed species. Both methods generated species-specific sequences or protein patterns for all species except for Phlebotomus pedifer and P. longipes, the two presumed vectors of Leishmania aethiopica, suggesting that they may represent a single species, P. longipes Parrot & Martin. All three approaches also revealed that the collected specimens of Adlerius sp. differ from P. (Adlerius) arabicus, the only species of Adlerius currently reported in Ethiopia, and molecular comparisons indicate that it may represent a yet undescribed new species. Conclusions Our study uses three complementary taxonomical methods for species identification of taxonomically challenging and yet medically import Ethiopian sand flies. The generated MALDI-TOF MS protein profiles resulted in unambiguous identifications, hence showing suitability of this technique for sand fly species identification. Furthermore, our results contribute to the still inadequate knowledge of the sand fly fauna of Ethiopia, a country severely burdened with human leishmaniasis.

Species identification of Streptococcus bovis group isolates causing bacteremia: a comparison of two MALDI-TOF MS systems

2017 ◽  
Vol 88 (1) ◽  
pp. 23-25 ◽  
Author(s):  
Charlotte N. Agergaard ◽  
Elisa Knudsen ◽  
Rimtas Dargis ◽  
Xiaohui C. Nielsen ◽  
Jens J. Christensen ◽  
...  
Keyword(s):  
Species Identification ◽  
Streptococcus Bovis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals The use of Principle Component Analysis and MALDI-TOF MS for the differentiation of mineral forming Virgibacillus and Bacillus species isolated from Sabkhas

2020 ◽  
Author(s):  
Rim Abdel Samad ◽  
Zulfa Al Disi ◽  
Mohammad Ashfaq ◽  
Nabil Zouari
Keyword(s):  
Clustering Analysis ◽  
Potential Role ◽  
Protein Profiling ◽  
Bacillus Species ◽  
Pca Analysis ◽  
Bacterial Strains ◽  
Protein Biomarkers ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Occurrence of mineral forming and other bacteria in mats is well demonstrated. However, their high diversity shown by ribotyping was not explained, although it could explain the diversity of formed minerals. Common biomarkers as well as phylogenic relationships are useful tools to clustering the isolates and predict their potential role in the natural niche. In this study, combination of MALDI-TOF MS with PCA was shown a powerful tool to categorize 35 mineral forming bacterial strains isolated from Dohat Fshaikh sabkha, at northwest of Qatar (23 from decaying mats and 12 from living ones). 23 strains from decaying mats belong to Virgibacillus genus as identified by ribotyping and are shown highly involved in formation of protodolomite and a diversity of minerals. They were used as internal references in categorization of sabkha bacteria. Combination of isolation of bacteria on selective mineral forming media, their MALDI TOF MS protein profiling and PCA analysis established their relationship in a phyloproteomic based on protein biomarkers including m/z 4905, 3265, 5240, 6430, 7765, and 9815. PCA analysis clustered the studied strains into 3 major clusters, showing strong correspondence to the 3 phyloproteiomic groups that were established by the dendrogram. Both clustering analysis means have evidently demonstrated a relationship between known Virgibacillus strains and other related bacteria based on profiling of their synthesized proteins. Thus, larger populations of bacteria in mats can be easily screened for their potential to exhibit certain activities, which is of ecological, environmental and biotechnological significance.

scholarly journals MALDI-TOF MS protein profiling for the rapid identification of Chagas disease triatomine vectors and application to the triatomine fauna of French Guiana – CORRIGENDUM

Parasitology ◽  
2017 ◽  
Vol 145 (5) ◽  
pp. 676-676 ◽  
Author(s):  
MAUREEN LAROCHE ◽  
JEAN-MICHEL BÉRENGER ◽  
GLADYS GAZELLE ◽  
DENIS BLANCHET ◽  
DIDIER RAOULT ◽  
...  
Keyword(s):  
Chagas Disease ◽  
French Guiana ◽  
Rapid Identification ◽  
Protein Profiling ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Thymosin β4 is differentially expressed in the cerebrospinal fluid of Creutzfeldt-Jakob disease patients: a MALDI-TOF MS protein profiling study

2009 ◽  
Vol 3 (5) ◽  
pp. 574-583 ◽  
Author(s):  
Antonio Qualtieri ◽  
Elena Urso ◽  
Maria Le Pera ◽  
Sabrina Bossio ◽  
Francesca Bernaudo ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Protein Profiling ◽  
Differentially Expressed ◽  
Thymosin Β4 ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Jakob Disease ◽  
Tof Ms

scholarly journals Species identification of enterobacteria strains by means of MALDI-TOF MS

2018 ◽  
pp. 110-113
Author(s):  
D.A. Vasiliev ◽  
◽  
N.A. Feoktistova ◽  
A.V. Mastilenko ◽  
E.V. Suldina ◽  
...  
Keyword(s):  
Species Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Novel attempt at discrimination of a bullet-shaped siphonophore (Family Diphyidae) using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-ToF MS)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Nayeon Park ◽  
Jisu Yeom ◽  
Raehyuk Jeong ◽  
Wonchoel Lee
Keyword(s):  
Species Identification ◽  
Morphological Characteristics ◽  
Kuroshio Current ◽  
Northwest Pacific ◽  
Maldi Tof Ms ◽  
Protein Mass ◽  
Maldi Tof ◽  
Morphological Findings ◽  
Flight Mass Spectrometry ◽  
Tof Ms

AbstractOne major difficulty in identifying the gelatinous bodied bullet-shaped Siphonophore, Diphyids, is that their shape is deformed following ethanol fixation. Ethanol often is preferred over other fixatives, since samples fixed in ethanol can be used for molecular studies that can supplement morphological findings. To overcome this problem, we obtained protein mass spectra of ten species of Diphyidae found in the waters of the Kuroshio Current (Northwest Pacific and South Coast of South Korea) to test whether MALDI-ToF MS could be used as a methodology for species identification. In addition, a number of morphological characteristics that can be used with ethanol-treated samples was summarized. Concatenated phylogenetic analysis was also performed to determine the phylogenetic relationship by obtaining partial sequences of four genes (mtCOI, 16S rRNA, 18S rRNA, and ITS regions). Based on our integrative analysis, MALDI-ToF MS was evaluated as a potentially fast, inexpensive, and accurate tool for species identification along with conventional morphological and DNA barcoding for Diphyidae.

scholarly journals Short Incubation of Positive Blood Cultures on Solid Media for Species Identification by MALDI-TOF MS: Which Agar Is the Fastest?

2021 ◽  
Author(s):  
Neele J. Froböse ◽  
Evgeny A. Idelevich ◽  
Frieder Schaumburg
Keyword(s):  
Mass Spectrometry ◽  
Species Identification ◽  
Susceptibility Testing ◽  
Solid Medium ◽  
Blood Cultures ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Solid Media ◽  
Laboratory Results ◽  
Tof Ms

When blood cultures are flagged as positive, they are incubated on solid media to produce enough biomass of the bacterium for identification and susceptibility testing. Rapid turnaround times for laboratory results could save lives, and we wanted to assess which solid medium is best to shorten the time to species identification using MALDI-TOF mass spectrometry.

scholarly journals Identification of Nocardia species using Autof MS 1000 and Bruker MALDI-TOF MS systems: A comparison

2021 ◽  
Author(s):  
Bing Ma ◽  
Yunqi Tian ◽  
Yungang Han ◽  
Lifang Ban ◽  
Junwen Yang ◽  
...  
Keyword(s):  
Species Identification ◽  
Protein Extraction ◽  
Reference Method ◽  
Invasive Disease ◽  
Species Level ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Significant Difference ◽  
Tof Ms

ABSTRACTNocardia is an important cause of clinically invasive disease, but for most clinical laboratories, identification of these isolates to the species level is challenging. Recently, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been widely used for identification of most bacterial and fungal isolates. In this multicenter study, we evaluated the identification of Nocardia isolates using Autof MS1000 and Bruker Biotyper. A total of 86 non-duplicate Nocardia isolates from 7 hospital laboratories were evaluated. Further, we carried out sequence analysis of 16S rRNA, gyrB, secA1, hsp65, and rpoB genes as a reference method for Nocardia species identification. The 86 isolates were directly spotted on the target plate and plate protein extraction was performed. Data were analyzed by SPSS 19.0. In total, 72 (83.7%) strains (score ≥ 9.0) and 70 (81.4%) strains (score ≥ 2.0) were correctly identified by the Autof MS1000 and Bruker Biotyper systems, respectively, at the species level. There was no significant difference (P > 0.05) between the two systems using the same protein extraction method. In conclusion, the Autof MS 1000 and Bruker MALDI-TOF systems showed no difference in identification of Nocardia spp. to the species level and could meet the most important clinical requirement for species identification.

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