scholarly journals Short Incubation of Positive Blood Cultures on Solid Media for Species Identification by MALDI-TOF MS: Which Agar Is the Fastest?

2021 ◽  
Author(s):  
Neele J. Froböse ◽  
Evgeny A. Idelevich ◽  
Frieder Schaumburg
Keyword(s):  
Mass Spectrometry ◽  
Species Identification ◽  
Susceptibility Testing ◽  
Solid Medium ◽  
Blood Cultures ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Solid Media ◽  
Laboratory Results ◽  
Tof Ms

When blood cultures are flagged as positive, they are incubated on solid media to produce enough biomass of the bacterium for identification and susceptibility testing. Rapid turnaround times for laboratory results could save lives, and we wanted to assess which solid medium is best to shorten the time to species identification using MALDI-TOF mass spectrometry.

scholarly journals Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization

2017 ◽  
Vol 55 (12) ◽  
pp. 3328-3338 ◽  
Author(s):  
Matthew L. Faron ◽  
Blake W. Buchan ◽  
Nathan A. Ledeboer
Keyword(s):  
Mass Spectrometry ◽  
Susceptibility Testing ◽  
Time Of Flight ◽  
Blood Cultures ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

ABSTRACTEarly initiation of effective antibiotics for septic patients is essential for patient survival. Matrix-assisted desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has revolutionized clinical microbiology for isolate identification and has the possibility to impact how blood culture testing is performed. This review discusses the various uses of MALDI-TOF MS for the identification and susceptibility testing of positive blood cultures, the performance of these methods, and the outcomes involved with its implementation.

scholarly journals Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0205603 ◽  
Author(s):  
Marlène Sauget ◽  
Xavier Bertrand ◽  
Didier Hocquet
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Blood Cultures ◽  
Antibiotic Susceptibility Testing ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Direct Rapid Identification from Positive Blood Cultures by MALDI-TOF MS: Specific Focus on Turnaround Times

2021 ◽  
Author(s):  
Hazan Zengin Canalp ◽  
Banu Bayraktar
Keyword(s):  
Blood Culture ◽  
Rapid Identification ◽  
Blood Cultures ◽  
Pathogen Identification ◽  
Short Term ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Solid Media ◽  
Time Required ◽  
Tof Ms

Using MALDI-TOF MS directly from blood culture bottles reduces the time required for pathogen identification, and the turnaround times for final identification have been compared with overnight incubation from solid media in previous studies. However, identification from a short incubation of agar plates has been increasingly accepted and successfully implemented in routine laboratories, but there is no data comparing direct MALDI-TOF MS with the short-term, incubated agar plates.

scholarly journals Rapid and cost-effective identification of Bartonella species using mass spectrometry

2009 ◽  
Vol 58 (9) ◽  
pp. 1154-1159 ◽  
Author(s):  
Pierre-Edouard Fournier ◽  
Carine Couderc ◽  
Sylvain Buffet ◽  
Christophe Flaudrops ◽  
Didier Raoult
Keyword(s):  
Mass Spectrometry ◽  
Species Identification ◽  
Chemical Reactivity ◽  
Bacterial Species ◽  
Cost Effective ◽  
Molecular Techniques ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Bacteria of the genus Bartonella are emerging zoonotic bacteria recognized in a variety of human diseases. Due to their poor chemical reactivity, these fastidious bacteria are poorly characterized using routine phenotypic laboratory tests. Identification is usually achieved using molecular techniques that are time-consuming, expensive and technically demanding. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a new technique for bacterial species identification. This study evaluated the use of MALDI-TOF MS for rapid genus and species identification of Bartonella species. Reference strains representing 17 recognized Bartonella species were studied. For each species, MS spectra for four colonies were analysed. The consensus spectrum obtained for each species was unique among spectra obtained for 2843 bacteria within the Bruker database, including 109 alphaproteobacteria. Thirty-nine additional blind-coded Bartonella strains were correctly identified at the species level, including 36 with a significant score. Altogether, these data demonstrate that MS is an accurate and reproducible tool for rapid and inexpensive identification of Bartonella species.

scholarly journals Clinical Impact of MALDI-TOF MS Identification and Rapid Susceptibility Testing on Adequate Antimicrobial Treatment in Sepsis with Positive Blood Cultures

PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0156299 ◽  
Author(s):  
Alexia Verroken ◽  
Lydwine Defourny ◽  
Olivier le Polain de Waroux ◽  
Leïla Belkhir ◽  
Pierre-François Laterre ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antimicrobial Treatment ◽  
Blood Cultures ◽  
Clinical Impact ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Correction: Clinical Impact of MALDI-TOF MS Identification and Rapid Susceptibility Testing on Adequate Antimicrobial Treatment in Sepsis with Positive Blood Cultures

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0160537 ◽  
Author(s):  
Alexia Verroken ◽  
Lydwine Defourny ◽  
Olivier le Polain de Waroux ◽  
Leïla Belkhir ◽  
Pierre-François Laterre ◽  
...  
Keyword(s):  
Susceptibility Testing ◽  
Antimicrobial Treatment ◽  
Blood Cultures ◽  
Clinical Impact ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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