Looking into ‘hair tonics’ for cotton fiber initiation

AbstractAuxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines fiber yield and quality. However, the regulation of this process is far from being well understood. In this study, we demonstrate an antagonistic effect between cytokinin (CK) and auxin on cotton fiber initiation. In vitro and in planta experiments indicate that enhanced CK levels can reduce auxin accumulation in the ovule integument, which may account for the defects in the fiberless mutant xu142fl. In turn, supplementation with auxin can recover fiber growth of CK-treated ovules and mutant ovules. We further found that GhPIN3a is a key auxin transporter for fiber-cell initiation and is polarly localized to the plasma membranes of non-fiber cells, but not to those of fiber cells. This polar localization allows auxin to be transported within the ovule integument while specifically accumulating in fiber cells. We show that CKs antagonize the promotive effect of auxin on fiber cell initiation by undermining asymmetric accumulation of auxin in the ovule epidermis through down-regulation of GhPIN3a and disturbance of the polar localization of the protein.

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GhJAZ2 negatively regulates cotton fiber initiation by interacting with the R2R3-MYB transcription factor GhMYB25-like

The Plant Journal ◽

10.1111/tpj.13273 ◽

2016 ◽

Vol 88 (6) ◽

pp. 921-935 ◽

Cited By ~ 52

Author(s):

Haiyan Hu ◽

Xin He ◽

Lili Tu ◽

Longfu Zhu ◽

Sitao Zhu ◽

...

Keyword(s):

Transcription Factor ◽

Cotton Fiber ◽

Myb Transcription Factor ◽

Fiber Initiation ◽

R2r3 Myb

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Characterization ofin vivophosphorylation modification of differentially accumulated proteins in cotton fiber-initiation process

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/gmw055 ◽

2016 ◽

Vol 48 (8) ◽

pp. 756-761 ◽

Cited By ~ 2

Author(s):

Wenying Liu ◽

Bing Zhang ◽

Wenying He ◽

Zi Wang ◽

Guanqiao Li ◽

...

Keyword(s):

Cotton Fiber ◽

Fiber Initiation ◽

Initiation Process

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Genome-Wide Analysis of AAT Genes and Their Expression Profiling during Fiber Development in Cotton

Plants ◽

10.3390/plants10112461 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2461

Author(s):

Dongjie Yang ◽

Yuanyuan Liu ◽

Hailiang Cheng ◽

Qiaolian Wang ◽

Limin Lv ◽

...

Keyword(s):

Cotton Fiber ◽

Biological Function ◽

Higher Plants ◽

Crop Improvement ◽

Fiber Development ◽

Maturity Stage ◽

Amino Acid Transporters ◽

Cotton Fiber Development ◽

Fiber Initiation ◽

Cotton Species

Amino acid transporters (AATs) are a kind of membrane proteins that mediate the transport of amino acids across cell membranes in higher plants. The AAT proteins are involved in regulating plant cell growth and various developmental processes. However, the biological function of this gene family in cotton fiber development is not clear. In this study, 190, 190, 101, and 94 full-length AAT genes were identified from Gossypiumhirsutum, G. barbadense, G. arboreum, and G. raimondii. A total of 575 AAT genes from the four cotton species were divided into two subfamilies and 12 clades based on phylogenetic analysis. The AAT genes in the four cotton species were distributed on all the chromosomes. All GhAAT genes contain multiple exons, and each GhAAT protein has multiple conserved motifs. Transcriptional profiling and RT qPCR analysis showed that four GhATT genes tend to express specifically at the fiber initiation stage. Eight genes tend to express specifically at the fiber elongation and maturity stage, and four genes tend to express specifically at the fiber initiation and elongation stages. Our results provide a solid basis for further elucidating the biological function of AAT genes related to cotton fiber development and offer valuable genetic resources for crop improvement in the future.

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Identification and Expression Pattern Analysis of Two Gossypium hirsutum Zinc Finger Transcription Factors During Cotton Fiber Initiation

National Academy Science Letters ◽

10.1007/s40009-019-00822-0 ◽

2019 ◽

Vol 43 (2) ◽

pp. 115-119

Author(s):

Rakesh Kumar ◽

Joy Das ◽

K. P. Raghavendra ◽

Sukhadeo B. Nandeshwar

Keyword(s):

Transcription Factors ◽

Gossypium Hirsutum ◽

Expression Pattern ◽

Zinc Finger ◽

Cotton Fiber ◽

Pattern Analysis ◽

Fiber Initiation ◽

Expression Pattern Analysis

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A Quantitative Electron Microscopic Study of Changes in Microtubule Arrays and Wall Microfibril Orientation During in vitro Cotton Fiber Development

Journal of Cell Science ◽

10.1242/jcs.101.3.561 ◽

1992 ◽

Vol 101 (3) ◽

pp. 561-577

Author(s):

ROBERT W. SEAGULL

Keyword(s):

Cotton Fiber ◽

Secondary Wall ◽

Fold Increase ◽

Fiber Development ◽

Electron Microscopic ◽

Cotton Fiber Development ◽

Fiber Initiation ◽

Microfibril Orientation ◽

Wall Deposition

A quantitative electron microscopic (E/M) study of the changes in microtubule arrays and wall microfibril orientation has been done on in vitro grown cotton fibers. Microtubules change orientation during cotton fiber development. During fiber initiation and early elongation, microtubules have a generally random orientation. Microtubules re-orient into shallow pitched helices as elongation and primary wall deposition continue, and into steeply pitched helices during secondary wall deposition. Accompanying the changes in orientation are increases in microtubule length, number, proximity to the plasmalemma and a decreased variability in orientation of the microtubules. Based on these observations, three pivotal stages in microtubule patterns were identified during fiber development: (1) the transition between fiber initiation and elongation, where microtubules develop a shallow pitched helical orientation; (2) the transition between primary and secondary wall synthesis, where microtubules abruptly shift orientation to a steeply pitched helical pattern; and (3) early in secondary wall synthesis, where there is a four fold increase in microtubule number. Microfibrils exhibit changes in orientation similar to the microtubules; however significant differences were found when the precise orientations of microtubules and microfibrils were compared. During secondary wall synthesis, wall microfibrils exhibit some variability in orientation due to inter-fibril bundling, thus indicating that components of the wall may also influence final microfibril orientation.

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