scholarly journals Sympathetic innervation of the interscapular brown adipose tissue in mouse

2019 ◽  
Vol 1454 (1) ◽  
pp. 3-13 ◽  
Author(s):  
Marie François ◽  
Hayden Torres ◽  
Clara Huesing ◽  
Rui Zhang ◽  
Carson Saurage ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Sympathetic Innervation ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose

Organization of sympathetic innervation of interscapular brown adipose tissue in the mouse

2021 ◽  
Author(s):  
Clara Huesing ◽  
Rui Zhang ◽  
Sanjeev Gummadi ◽  
Nathan Lee ◽  
Emily Qualls‐Creekmore ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Sympathetic Innervation ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose

Role of the sympathetic innervation in the cold-induced activation of 5′-deiodinase in brown adipose tissue of the Djungarian hamster

1991 ◽  
Vol 69 (12) ◽  
pp. 1896-1900 ◽  
Author(s):  
Anette Meywirth ◽  
Uwe Redlin ◽  
Stephan Steinlechner ◽  
Gerhard Heldmaier ◽  
Russel J. Reiter
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Sympathetic Innervation ◽  
Djungarian Hamster ◽  
Control Groups ◽  
Interscapular Brown Adipose Tissue ◽  
Deiodinase Activity ◽  
Brown Adipose ◽  
Norepinephrine Content

The importance of the sympathetic innervation in the regulation of 5′-deiodinase activity in the interscapular brown adipose tissue (BAT) of the Djungarian hamster was studied. Interscapular BAT of Djungarian hamsters was either unilaterally or bilaterally denervated, and thereafter the animals were maintained at thermoneutral temperature or exposed to 0 °C for 24 h. Denervation reduced the norepinephrine content to 2–10% of the level in the control groups. Unilateral denervation was as effective as bilateral denervation in depressing the norepinephrine content of the interscapular BAT. Cold exposure for 24 h resulted in a pronounced 5′-deiodinase activation. Denervation reduced, but did not completely prevent, the cold-induced increase in 5′-deiodinase activity. The basal level of 5′-deiodinase activity at thermoneutral temperature was not reduced by denervation. We conclude that cold-induced activation of BAT 5′-deiodinase primarily depends on the intact sympathetic innervation.Key words: nonshivering thermogenesis, brown fat, 5′-deiodinase, Phodopus sungorus.

Role of ventromedial hypothalamus on sympathetic efferents of brown adipose tissue

1984 ◽  
Vol 247 (4) ◽  
pp. R650-R654 ◽  
Author(s):  
A. Niijima ◽  
F. Rohner-Jeanrenaud ◽  
B. Jeanrenaud
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Spontaneous Activity ◽  
Sympathetic Innervation ◽  
Sympathetic Nerves ◽  
Ventromedial Hypothalamus ◽  
Functional Link ◽  
Interscapular Brown Adipose Tissue ◽  
Cold Stimulus ◽  
Brown Adipose

Previous studies have suggested the presence, in hypothalamic obesity, of an impairment of the energy-dissipating capacity of brown adipose tissue ascribed to a functional disconnection of the sympathetic innervation of this tissue. The following observations demonstrate, with electrophysiological techniques, the presence of a functional link between the ventromedial hypothalamic (VMH) area and the interscapular brown adipose tissue (IBAT) in the rat: the spontaneous activity of the efferent sympathetic nerves reaching the IBAT of normal rats was increased in response to an acute cold stimulus, whereas this increase failed to occur in nerves of VMH-lesioned rats studied 4–7 days after the lesions; and the spontaneous activity of the efferent sympathetic nerves of IBAT decreased rapidly (by greater than or equal to 80% within 30 min) after acute lesions of the VMH area. It is suggested that the VMH area plays a role in increasing the activity of the efferent sympathetic nerves of IBAT during an acute cold stimulus and that alone or in relationship with other, as yet undetermined, central nervous system sites, it has a tonic stimulatory effect on the final common pathways that innervate the IBAT via the efferent sympathetic nerves.

Sedimentation characteristics of noradrenergic vesicles from rat interscapular brown adipose tissue

1982 ◽  
Vol 60 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Gloria Zaror-Behrens ◽  
Florent Depocas ◽  
Suzanne Lacelle
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Fast Moving ◽  
Microsomal Fraction ◽  
Sympathetic Innervation ◽  
Supernatant Fraction ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose ◽  
Slow Moving ◽  
Differential Sedimentation

Rat interscapular brown adipose tissue (IBAT) was homogenized to release the noradrenergic vesicles present in its dense sympathetic innervation. The vesicles were then studied by several sedimentation techniques using noradrenaline (NA) and dopamine β-hydroxylase (DBH) as markers. Sixty-three percent of the DBH activity and 29% of the NA in homogenates (0.25 M sucrose, 5 mM Tris, pH 7.4 at 21 °C) of IBAT from 28 °C acclimated rats sedimented in the microsomal fraction (226 600 × gmax, 60 min). Differential sedimentation of the microparticulate DBH in a low-speed supernatant fraction of the homogenate indicated at least two distinct populations of microparticles with average sedimentation coefficients of 80 ± 11 and [Formula: see text] (4 °C) and containing, respectively, about 65 and 35% of the sedimentable DBH. Upon isopycnic, sucrose density centrifugation of the resuspended microsomal fraction, DBH peaked at a density of 1.091 but extended as a broad shoulder up to a density of about 1.19. During rate zonal centrifugation of the resuspended microsomal fraction on sucrose density gradients, microparticulate DBH and NA separated into slow and fast moving components. The modal density of the slow moving component upon isopycnic recentrifugation was 1.092, while the fast moving one, similarly treated, became almost equally distributed over a range of densities from 1.12 to 1.19. For the slow moving component, NA and DBH relative to protein were, respectively, 6.5 and 23 times more concentrated than in the IBAT homogenate. On the basis of its measured sedimentation characteristics, the slow moving component would correspond to vesicles having a calculted diameter of 66 nm. The data thus indicate that in IBAT, DBH and NA can be separated into two distinct populations of sedimentable particles. Whether or not these correspond to the small and large dense-cored vesicles observed by ultramicroscopy of IBAT remains to be demonstrated.

Heterogeneity of the sympathetic innervation of rat interscapular brown adipose tissue via intercostal nerves

1982 ◽  
Vol 60 (6) ◽  
pp. 747-754 ◽  
Author(s):  
David O. Foster ◽  
Florent Depocas ◽  
Michael Zuker
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Sympathetic Innervation ◽  
Intercostal Nerve ◽  
Tissue Blood Flow ◽  
Posterior Lobe ◽  
Interscapular Brown Adipose Tissue ◽  
Brown Adipose ◽  
Intercostal Nerves ◽  
Stimulation Of

The interscapular brown adipose tissue (IBAT) of cold-acclimated white rats was partially denervated by cutting in most cases four of the five intercostal nerves going to one of its two bilaterally located, lobular pads. After 24 h the functional ability and the degree of residual sympathetic innervation of the pads and of their lobes were assessed (in separate groups of rats): the former by measuring tissue blood flow as an index of sympathetically mediated calorigenesis during exposure of the animals to −6 °C, the latter by measuring the residual noradrenaline (NA) content of the tissue. Only the four anterior intercostal nerves contributed significantly to the sympathetic innervation of IBAT pads. On average their contributions were unequal, but there was considerable variation between rats in the relative contribution of each nerve. The measurements on lobes of the pads indicated that the sympathetic fibers supplied by a given intercostal nerve were not distributed uniformly throughout the pad. For example, measurements of NA indicated that on average the fibers of the fourth nerve were four times as abundant in the lateral lobe as in the posterior lobe. But at this level of analysis also there was wide variation between rats. The variation in the participation of each intercostal nerve in the sympathetic innervation of rat IBAT and in the distribution of each nerve's fibers to different parts of the IBAT pad could be of genetic origin or it could reflect a considerable degree of flexibility during development of the innervation of IBAT. Because of the observed variation, it is suggested that if functional responses of IBAT evoked by electrical stimulation of intercostal nerves are to be recorded from a highly localized area of the tissue, stimulation of all the nerves probably would be required to obtain responses that are consistent between animals.

Unilaterality of the sympathetic innervation of each pad of rat interscapular brown adipose tissue

1982 ◽  
Vol 60 (2) ◽  
pp. 107-113 ◽  
Author(s):  
David O. Foster ◽  
Florent Depocas ◽  
Gloria Zaror Behrens
Keyword(s):  
Blood Flow ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Cold Exposure ◽  
Sympathetic Innervation ◽  
Maximum Increase ◽  
Interscapular Brown Adipose Tissue ◽  
Tissue Levels ◽  
Brown Adipose ◽  
Low Levels

The effects of sham, unilateral, and bilateral surgical denervation of rat interscapular brown adipose tissue (IBAT) on blood flow to the two IBAT pads of cold-acclimated (CA) rats during exposure of the animals to 22 or −6 °C and on the noradrenaline (NA) content and total dopamine-β-hydroxylase (DBH) (EC 1.14.17.1) activity of the pads in both warm-acclimated (WA) rats and CA rats were examined. Increase in IBAT blood flow upon cold exposure was taken as an index of sympathetically mediated calorigenesis in the tissue, and decreases in tissue levels of NA and DBH served as indices of the extent of destruction of the sympathetic innervation. At 24 h postsurgery, denervated pads of CA rats, whether from unilaterally or bilaterally denervated IBAT, had less than 3% of the NA, 40-44% of the DBH, and 0% of the 10-fold, cold-induced increase in blood flow measured in intact pads of CA rats with sham-operated or unilaterally denervated IBAT. IBAT bilaterally denervated for 24 h was as responsive in terms of its maximum increase in blood flow during infusion of CA rats with NA as intact IBAT. DBH in denervated pads of both WA rats and CA rats fell to 5% or less of control levels at 2 days postdenervation and remained at these low levels, as did NA, for at least 8 weeks. These results strongly support the longstanding but recently challenged hypothesis that each pad of rat IBAT is independently innervated by sympathetic fibers.

Sign in / Sign up

Export Citation Format

Share Document