scholarly journals Enhanced resistance inTheobroma cacaoagainst oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins

2015 ◽  
Vol 14 (3) ◽  
pp. 875-886 ◽  
Author(s):  
Emily E. Helliwell ◽  
Julio Vega-Arreguín ◽  
Zi Shi ◽  
Bryan Bailey ◽  
Shunyuan Xiao ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Binding Proteins ◽  
Phosphate Binding ◽  
Enhanced Resistance ◽  
Phosphatidylinositol 3

Regulation of early-endosome dynamics by phosphatidylinositol 3-phosphate binding proteins

1999 ◽  
Vol 27 (4) ◽  
pp. 662-666 ◽  
Author(s):  
M. J. Clague ◽  
A. T. Jones ◽  
I. G. Mills ◽  
D. M. Walker ◽  
S. Urbé
Keyword(s):  
Binding Proteins ◽  
Early Endosome ◽  
Phosphate Binding ◽  
Phosphatidylinositol 3

scholarly journals Mutations in Wheat Exhibiting Growth-Stage-Specific Resistance to Biotrophic Fungal Pathogens

2004 ◽  
Vol 17 (11) ◽  
pp. 1242-1249 ◽  
Author(s):  
Phil H. Smith ◽  
John A. Howie ◽  
Anthony J. Worland ◽  
Rebecca Stratford ◽  
Lesley A. Boyd
Keyword(s):  
Fungal Pathogens ◽  
Growth Stage ◽  
Puccinia Striiformis ◽  
Specific Resistance ◽  
Yellow Rust ◽  
Dominant Mutation ◽  
Resistance Phenotype ◽  
The Third ◽  
Enhanced Resistance ◽  
Seedling Leaf

Two mutants were isolated in wheat that showed enhanced resistance towards Puccinia striiformis f. sp. tritici, the fungal causal agent of yellow rust. The altered phenotype of I3-48 is due to a minimum of two mutation events, each showing a partial, additive effect, with one mutation segregating with a deletion on the long arm of chromosome 4D. In the case of I3-54, the enhanced resistance is due to a single, dominant mutation. In both mutants, the expression of the enhanced resistance is growth-stage specific. With I3-54, the full resistance phenotype is apparent from the third seedling leaf onwards, while with I3-48, a full resistance phenotype is only seen on the tenth and subsequent leaves. In addition to the enhanced resistance towards yellow rust, I3-48 also shows enhanced resistance towards brown rust, and I3-54 shows enhanced resistance to powdery mildew.

scholarly journals Characterization of VPS34-IN1, a selective inhibitor of Vps34, reveals that the phosphatidylinositol 3-phosphate-binding SGK3 protein kinase is a downstream target of class III phosphoinositide 3-kinase

2014 ◽  
Vol 463 (3) ◽  
pp. 413-427 ◽  
Author(s):  
Ruzica Bago ◽  
Nazma Malik ◽  
Michael J. Munson ◽  
Alan R. Prescott ◽  
Paul Davies ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Selective Inhibitor ◽  
Class Iii ◽  
Phosphate Binding ◽  
Downstream Target ◽  
Phosphoinositide 3 Kinase ◽  
Phosphatidylinositol 3

We characterize VPS34-IN, a potent and selective inhibitor of class III Vps34 PI3K. Using VPS34-IN1, we demonstrate that PtdIns(3)P, produced by Vps34 controls phosphorylation and activity of the SGK3 protein kinase.

scholarly journals Phosphate removal and recovery using immobilized phosphate binding proteins

2018 ◽  
Vol 1 ◽  
pp. 100003 ◽  
Author(s):  
Kaushik Venkiteshwaran ◽  
Nilisha Pokhrel ◽  
Faten Hussein ◽  
Edwin Antony ◽  
Brooke K. Mayer
Keyword(s):  
Binding Proteins ◽  
Phosphate Removal ◽  
Phosphate Binding ◽  
Removal And Recovery

scholarly journals Expression of the Membrane-Associated Resistance Protein RPW8 Enhances Basal Defense Against Biotrophic Pathogens

2007 ◽  
Vol 20 (8) ◽  
pp. 966-976 ◽  
Author(s):  
Wenming Wang ◽  
Alessandra Devoto ◽  
John G. Turner ◽  
Shunyuan Xiao
Keyword(s):  
Salicylic Acid ◽  
Powdery Mildew ◽  
Fungal Pathogens ◽  
Coiled Coil ◽  
Small Gtpase ◽  
Cauliflower Mosaic Virus ◽  
R Genes ◽  
Amino Terminal ◽  
Small Proteins ◽  
Enhanced Resistance

The powdery mildew resistance genes RPW8.1 and RPW8.2 from Arabidopsis differ from the other isolated plant resistance (R) genes in their predicted protein domains and their resistance spectrum. The two homologous RPW8 genes encode small proteins featuring a predicted amino-terminal transmembrane anchor domain and a coiled-coil domain and confer resistance to a broad spectrum of powdery mildews. Here, we show that Arabidopsis plants expressing the RPW8 genes have enhanced resistance to another biotrophic pathogen, Hyaloperonospora parasitica, raising the possibility that the RPW8 genes may function to enhance salicylic-acid-dependent basal defenses, rather than as powdery-mildew-specific R genes. When overexpressed from their native promoters, the RPW8 genes confer enhanced resistance to the Cauliflower mosaic virus, but render plants more susceptible to the necrotrophic fungal pathogens Alternaria and Botrytis spp. Furthermore, we show that the RPW8 proteins appear to be localized to the endomembrane system, overlapping with the endoplasmic reticulum–associated small GTPase SAR1, and accumulate to higher levels in response to application of exogenous salicylic acid, one of the signaling molecules of plant defense.

scholarly journals Gel Shift Assay of Nuclear Extracts from Histoplasma capsulatum Demonstrates the Presence of Several DNA Binding Proteins

2002 ◽  
Vol 70 (4) ◽  
pp. 2238-2241 ◽  
Author(s):  
Atanas Ignatov ◽  
Elizabeth J. Keath
Keyword(s):  
Dna Binding ◽  
Fungal Pathogens ◽  
Binding Proteins ◽  
Histoplasma Capsulatum ◽  
Protein S ◽  
Dna Binding Proteins ◽  
Mobility Shift ◽  
Nuclear Extracts ◽  
Gel Shift Assay ◽  
Gel Shift

ABSTRACT A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.

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