Impact of Humoral Immune Response and Absorption Effect on Dynamics of Dengue Virus

Dengue infection represents a global threat causing 50-100 million infections per year and placing half of the world’s population at risk. Even though how infection is controlled and cured rather remains a mystery, antibodies are thought to play a major role in clearing the virus. In this paper, we study the dynamics of dengue virus with humoral immune response and absorption effect. The proposed model incorporates a time delay in production of antibodies. The basic reproduction number R0 is computed and a detailed stability analysis is done. It was found that the model has 3 steady states, namely, infection free equilibrium, no immune equilibrium and the endemic equilibrium. Conditions for R0 were developed for the local stability of these 3 equilibrium states. The global stability was studied using appropriate Lyapunov function and LaSalle’s invariance principle. We then established a condition for which the endemic equilibrium point is globally asymptotically stable. Also it was observed that the virus count goes to negligible levels within 7-14 days after the onset of symptoms.

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Simulation Model for Dynamics of Dengue with Innate and Humoral Immune Responses

Computational and Mathematical Methods in Medicine ◽

10.1155/2018/8798057 ◽

2018 ◽

Vol 2018 ◽

pp. 1-18 ◽

Cited By ~ 3

Author(s):

S. D. Perera ◽

S. S. N. Perera

Keyword(s):

Immune Response ◽

Immune Responses ◽

Dengue Virus ◽

Dengue Infection ◽

Asymptomatic Infection ◽

Severe Dengue ◽

Viral Kinetics ◽

Humoral Immune ◽

Humoral Immune Responses ◽

Kinetics Of

Dengue virus is a mosquito borne Flavivirus and the most prevalent arbovirus in tropical and subtropical regions around the world. The incidence of dengue has increased drastically over the last few years at an alarming rate. The clinical manifestation of dengue ranges from asymptomatic infection to severe dengue. Even though the viral kinetics of dengue infection is lacking, innate immune response and humoral immune response are thought to play a major role in controlling the virus count. Here, we developed a computer simulation mathematical model including both innate and adaptive immune responses to study the within-host dynamics of dengue virus infection. A sensitivity analysis was carried out to identify key parameters that would contribute towards severe dengue. A detailed stability analysis was carried out to identify relevant range of parameters that contributes to different outcomes of the infection. This study provides a qualitative understanding of the biological factors that can explain the viral kinetics during a dengue infection.

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ANALYSIS OF RECOMBINANT DENGUE VIRUS POLYPEPTIDES FOR DENGUE DIAGNOSIS AND EVALUATION OF THE HUMORAL IMMUNE RESPONSE

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.2004.71.144 ◽

2004 ◽

Vol 71 (2) ◽

pp. 144-152 ◽

Cited By ~ 15

Author(s):

FLAVIA BARRETO DOS SANTOS ◽

EVA HARRIS ◽

RITA MARIA RIBEIRO NOGUEIRA ◽

LEE W. RILEY ◽

MARIZE PEREIRA MIAGOSTOVICH ◽

...

Keyword(s):

Immune Response ◽

Dengue Virus ◽

Humoral Immune Response ◽

Humoral Immune

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Hubungan Respon Imun Humoral dengan Derajat Trombositopenia pada Pasien Demam Berdarah Dengue

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v4i1.2010.36-41 ◽

2017 ◽

Vol 4 (1) ◽

pp. 36

Author(s):

Fatmawati Fatmawati ◽

Chandra Wijaya

Keyword(s):

Immune Response ◽

Dengue Hemorrhagic Fever ◽

Humoral Immune Response ◽

Analytical Study ◽

Dengue Infection ◽

Hemorrhagic Fever ◽

Statistical Test ◽

Cross Sectional ◽

Humoral Immune ◽

Fever Patient

The correlation between humoral immune response with the level of of thrombocytopenia on dengue hemorrhagicfever patient The aim of this study was to know the correlation between humoral immune response with the level of ofthrombocytopenia on dengue hemorrhagic fever patient. This is a cross sectional analytical study on internal inpatientsof AwalBros Pekanbaru Hospital in August-November 2008. Population was all internal inpatients of AwalBros PekanbaruHospital with working diagnosis dengue hemorrhagic fever. There were 4 pattern of anti Dengue antibody : IgG-/IgM,IgG-/IgM+, IgG+/IgM-, IgG+/IgM+. Most of patients with IgG+/IgM+ and have secondary dengue infection Thoseresult were analyzed by Friedman statistical test. There isn’t a significant correlation between the level ofthrombocytopenia and anti dengue antibody. It show that there wasn’t a direct correlation between them. It could bedue to a cross reaction between anti dengue antibody with thrombocyte (autoantibody).

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Production of dengue virus-like particles serotype-3 in silkworm larvae and their ability to elicit a humoral immune response in mice

AMB Express ◽

10.1186/s13568-020-01087-3 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Doddy Irawan Setyo Utomo ◽

Sabar Pambudi ◽

Fithriyah Sjatha ◽

Tatsuya Kato ◽

Enoch Y. Park

Keyword(s):

Immune Response ◽

Dengue Virus ◽

Humoral Immune Response ◽

Silkworm Larvae ◽

Virus Like Particles ◽

Humoral Immune

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Immunogenicity of Recombinant DNA Vaccine Encoding Non-Structural Protein-1 Dengue Virus Serotype-2 in Balb/c Mice

Microbiology Indonesia ◽

10.5454/mi.15.2.4 ◽

2021 ◽

Vol 15 (2) ◽

pp. 4

Author(s):

Elitha Pulungan

Keyword(s):

Immune Response ◽

Dengue Virus ◽

Dna Vaccine ◽

Humoral Immune Response ◽

Structural Proteins ◽

Ns1 Protein ◽

Structural Protein ◽

Humoral Immune ◽

Virus Serotype ◽

Positive Strand Rna

Background: Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus (DENV) which spread widely in tropical and subtropical regions of the world. DENV is a single-positive strand RNA virus with a genome size of ± 11kb which encodes three structural proteins, seven non-structural proteins, and two untranslated regions (UTR). The non-structural protein-1 (NS1) of DENV is known to have important role in dengue pathogenesis also promising to be developed as dengue vaccine. Lately, novel vaccine approach by DNA immunization have given new perspective for a safe, stable, and immunogenic vaccine platform. Previously, we have successfully construct DNA vaccine encoding NS1 protein of DENV2 (pUNS1) which express recombinant NS1 protein in-vitro. Thus, in this current study the ability of pUNS1 to induce humoral immune response will be further analyzed by in mice immunization. Methods: Sixteen BALB/c mice aged of 4 weeks were immunized 3 times with 100 µg of pUNS1 or pUMVC4a on 2 week time interval. Blood sampling was carried out just before immunization and termination was done 2 week after last immunization. Titer from individual mice sera against DENV-2 were measure with in-house ELISA. Results: IgG against NS1 protein of DENV2 titer from mice group immunized with recombinant pUNS1 shown high ELISA absorbancies, 5 times higher than pUMVC4a group. This result suggest the ability of pUNS1 to induce humoral immune response against NS1 DENV-2 in-vivo. Conclusion: Recombinant pUNS1 can induce humoral immune response in mice.

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