Optimizing embryo age and media for enhancing hybrid seedling recovery in sour orange ( Citrus aurantium ) × Sacaton citrumelo ( C. paradisi  ×  Poncirus trifoliata ) crosses through embryo rescue

2019 ◽  
Vol 138 (3) ◽  
pp. 368-376 ◽  
Author(s):  
Anjali Soni ◽  
Anil Kumar Dubey ◽  
Ankit Gupta ◽  
Radha Mohan Sharma ◽  
Om Prakash Awasthi ◽  
...  
2003 ◽  
Vol 54 (6) ◽  
pp. 571 ◽  
Author(s):  
I. E. Papadakis ◽  
K. N. Dimassi ◽  
I. N. Therios

A greenhouse experiment was performed to study the effects of boron (B) on growth, nutrient concentration and distribution, nutrient use efficiency, and total nutrient absorption of 2 citrus genotypes. The experimental layout was a 6 × 2 factorial, with 6 B concentrations (0.05, 0.25, 0.50, 1.00, 2.00, and 5.00 mg/L) on 2 genotypes: the sour orange (Citrus aurantium L.) and the Swingle citrumelo (C. paradisi Macf. × Poncirus trifoliata L.). The plants were grown for 3 months in a B-free sand : perlite (1 : 1) medium that was irrigated with 6 half-strength Hoagland's nutrient solutions. Increasing B supply in the nutrient solution increased the B concentration linearly in all parts of the plant in the following order: basal leaves > top leaves > bark > root > stems > wood. There was no consistent effect of B supply on the concentration of other fundamental elements (P, K, Ca, Mg, Mn, Zn, Fe). Furthermore, none of the tested B concentrations significantly affected the total plant content and consequently the absorption of any other element. A concentration of 1.00 mg B/L or higher resulted in less B absorption by the Swingle citrumelo than by the sour orange. Furthermore, the Swingle citrumelo has the ability to retain more B in its stems and roots than the sour orange, thus preventing B transport to leaves. Finally, B and Mn use efficiency in both genotypes correlated significantly and negatively with the B supply.


2021 ◽  
pp. 1-13
Author(s):  
Louloudia Koulympoudi ◽  
Anna Papafilippou ◽  
Maria Tzanoudaki ◽  
Christos Chatzissavvidis ◽  
Vasileios Salamalikis

Fruits ◽  
2011 ◽  
Vol 66 (5) ◽  
pp. 315-325 ◽  
Author(s):  
Inès Ellouze ◽  
Hajer Debbabi ◽  
Ameni Belkacem ◽  
Boulbeba Rekik

2012 ◽  
Vol 11 (3) ◽  
pp. 3267-3276 ◽  
Author(s):  
I. Polat ◽  
Y.A. Kaçar ◽  
T. Yesiloglu ◽  
A. Uzun ◽  
O. Tuzcu ◽  
...  

1998 ◽  
Vol 88 (5) ◽  
pp. 389-395 ◽  
Author(s):  
T. L. Widmer ◽  
J. H. Graham ◽  
D. J. Mitchell

Phytophthora nicotianae and P. palmivora infect and cause rot of fibrous roots of susceptible and tolerant citrus rootstocks in Florida orchards. The infection and colonization by the two Phytophthora spp. of a susceptible citrus host, sour orange (Citrus aurantium), and a tolerant host, trifoliate orange (Poncirus trifoliata), were compared using light and electron microscopy. Penetration by both Phytophthora spp. occurred within 1 h after inoculation, regardless of the host species. No differences were observed in mode of penetration of the hypodermis or the hosts' response to infection. After 24 h, P. palmivora had a significantly higher colonization of cortical cells in susceptible sour orange than in tolerant trifoliate orange. Intracellular hyphae of both Phytophthora spp. were observed in the cortex of sour orange, and cortical cells adjacent to intercellular hyphae of P. palmivora were disrupted. In contrast, the cortical cells of sour orange and trifoliate orange adjacent to P. nicotianae hyphae and the cortical cells of trifoliate orange adjacent to P. palmivora were still intact. After 48 h, the cortical cells of both hosts adjacent to either Phytophthora spp. were disrupted. After 48 and 72 h, P. palmivora hyphae colonized the cortex of sour orange more extensively than the cortex of trifoliate orange; P. palmivora also colonized both hosts more extensively than P. nicotianae. A higher rate of electrolyte leakage among host-pathogen combinations reflected the combined effects of greater cell disruption by P. palmivora than by P. nicotianae, and the higher concentration of electrolytes in healthy roots of trifoliate orange than of sour orange. Although cellular responses unique to the tolerant host were not observed, reduced hyphal colonization by both pathogens in the cortex of trifoliate orange compared with sour orange is evidence for a putative resistance factor(s) in the trifoliate orange roots that inhibits the growth of Phytophthora spp.


2007 ◽  
Vol 34 (8) ◽  
pp. 720 ◽  
Author(s):  
Fabio Raimondo ◽  
Francesco Raudino ◽  
Santa Olga Cacciola ◽  
Sebastiano Salleo ◽  
Maria Assunta Lo Gullo

Phoma trachephila (Petri) Kantschaveli et Gikachvili causes dieback of several Citrus species. The impact of this fungus on leaf hydraulics was studied in Citrus aurantium L. (sour orange) with the aim of identifying the primary mechanism of damage to leaves. Leaves inoculated with a conidial suspension were measured for conductance to water vapor (gL) and specific hydraulic conductance (Kleaf) every 3 days after inoculation. The earliest symptom of infection consisted of vein chlorosis. Functional vein density (FVD) was monitored and microscopic observations were made of major vein conduits. Impairment of vein hydraulics started 25 days after inoculation with a losses of Kleaf of 40% and gL of ~60%. Most minor veins within chlorotic areas were no longer functioning and some conduits of the major veins showed digested interconduit pits leading to vein cavitation. The close Kleaf–FVD relationship revealed that vein impairment caused drop of Kleaf and, consequently, of gL at chlorotic areas. Leaf infection was focused to veins that were first forced to embolise and then invaded by fungal hyphae. The vein embolism due to the Phoma amplifies the native dominant hydraulic resistance of leaf veins, and leads ultimately to early shedding of infected leaves.


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