scholarly journals A multiplex PCR marker distinguishes between a series of four LanFTc1 alleles regulating flowering time in narrow‐leafed lupin ( Lupinus angustifolius )

2021 ◽  
Author(s):  
Candy M. Taylor ◽  
Julian Zanden ◽  
Renu Saradadevi ◽  
Jens D. Berger ◽  
Lars G. Kamphuis ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Flowering Time ◽  
Lupinus Angustifolius ◽  
Pcr Marker

scholarly journals Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR

2021 ◽  
Vol 16 (1) ◽  
pp. 172-183
Author(s):  
Agnieszka Tomkowiak ◽  
Roksana Skowrońska ◽  
Michał Kwiatek ◽  
Julia Spychała ◽  
Dorota Weigt ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Leaf Rust ◽  
Common Wheat ◽  
Fungal Pathogens ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Field Conditions ◽  
Pcr Marker ◽  
Specific Sequence ◽  
Cereal Species

Abstract Leaf rust caused by the fungus Puccinia recondita f. sp. tritici is one of the most dangerous diseases of common wheat. Infections caused by fungal pathogens reduce the quantity and quality of yields of many cereal species. The most effective method to limit plant infection is to use cultivars that show rust resistance. Genetically conditioned horizontal-type resistance (racial-nonspecific) is a desirable trait because it is characterized by more stable expression compared to major (R) genes that induce racially specific resistance, often overcome by pathogens. Horizontal resistance is conditioned by the presence of slow rust genes, which include genes Lr34 and Lr46. This study aimed to identify markers linked to both genes in 64 common wheat lines and to develop multiplex PCR reaction conditions that were applied to identify both genes simultaneously. The degree of infestation of the analyzed lines was also assessed in field conditions during the growing season of 2017 and 2018. Simple sequence repeat anchored-polymerase chain reaction (SSR-PCR) marker csLV was identified during analysis in line PHR 4947. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. In addition to gene Lr34, gene Lr46 was identified in this genotype. Lines PHR 4947 and PHR 4819 were characterized by the highest leaf rust resistance in field conditions. During STS-PCR analyses, the marker wmc44 of gene Lr46 was identified in most of the analyzed lines. This marker was not present in the following genotypes: PHR 4670, PHR 4800, PHR 4859, PHR 4907, PHR 4922, PHR 4949, PHR 4957, PHR 4995, and PHR 4997. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. Genotypes carrying the markers of the analyzed gene showed good resistance to leaf rust in field conditions in both 2017 and 2018. Research has demonstrated that marker assisted selection (MAS) and multiplex PCR techniques are excellent tools for selecting genotypes resistant to leaf rust.

Daylength responses, flowering time, and seed filling in lupins

1998 ◽  
Vol 49 (7) ◽  
pp. 1047 ◽  
Author(s):  
Miles Dracup ◽  
Bob Thomson ◽  
Mark Reader ◽  
E. J. M. Kirby ◽  
Ian Shield ◽  
...  
Keyword(s):  
Flowering Time ◽  
Lupinus Angustifolius ◽  
Seed Filling ◽  
Artificial Lighting ◽  
Target Environment ◽  
The Uk ◽  
New Crop ◽  
Short Days

Narrow-leafed lupin (Lupinus angustifolius) is now an established major crop in southern Australia, albus lupin (L. albus) is grown on a smaller scale, and there is interest in cultivating other lupin species. With any new crop, it is crucial that its phenology, particularly times of flowering and maturity, matches the target environment. We investigated times of flowering, maturity, and seed filling of several lupin species of agricultural interest to southern Australia. Established genotypes of L. angustifolius and L. albus flowered earliest, in 70-75 days, but L. luteus cv. Teo and some L. angustifolius lines with restricted branching matured earliest. Differences between genotypes in rates of seed filling and final seed weights meant that time of maturity was only poorly correlated with flowering time. We investigated the role of photoperiod in the timing of flowering and maturity in 12 lupin species/genotypes by: (a) growing plants under both long days in the UK and short days in Australia; and (b) growing plants under short days in Australia with or without lighting to extend the photoperiod. Flowering was clearly hastened by long days in all genotypes in both situations. However, the responses were much greater when days were naturally longer than when they were artificially lengthened. This was probably due to the illuminance requirements for perception of photoperiod by lupin not being saturated by the artificial lighting.

scholarly journals Genetic and comparative mapping of Lupinus luteus L. highlight syntenic regions with major orthologous genes controlling anthracnose resistance and flowering time

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Nicole Lichtin ◽  
Haroldo Salvo-Garrido ◽  
Bradley Till ◽  
Peter D. S. Caligari ◽  
Annally Rupayan ◽  
...  
Keyword(s):  
Flowering Time ◽  
Genetic Improvement ◽  
Early Flowering ◽  
Lupinus Luteus ◽  
Phenotypic Variance ◽  
Orthologous Genes ◽  
Pcr Marker ◽  
Anthracnose Resistance ◽  
Genomic Regions ◽  
Syntenic Regions

Abstract Anthracnose susceptibility and ill-adapted flowering time severely affect Lupinus luteus yield, which has high seed protein content, is excellent for sustainable agriculture, but requires genetic improvement to fulfil its potential. This study aimed to (1) develop a genetic map; (2) define collinearity and regions of synteny with Lupinus angustifolius; and (3) map QTLs/candidate genes for anthracnose resistant and flowering time. A few linkage groups/genomic regions tended to be associated with segregation distortion, but did not affect the map. The developed map showed collinearity, and syntenic regions with L. angustifolius. Major QTLs were mapped in syntenic regions. Alleles from the wild parent and cultivar, explained 75% of the phenotypic variance for anthracnose resistance and 83% for early flowering, respectively. Marker sequences flanking the QTLs showed high homology with the Lanr1 gene and Flowering-locus-T of L. angustifolius. This suggests orthologous genes for both traits in the L. luteus genome. The findings are remarkable, revealing the potential to combine early flowering/anthracnose resistant in fulfilling yield capacity in L. luteus, and can be a major strategy in the genetic improvement and usage of this species for sustainable protein production. Allele sequences and PCR-marker tagging of these genes are being applied in marker assisted selection.

Multiplex PCR effectively identifies tetraploid Triticum AABB – or AAGG-genome species

2017 ◽  
Vol 16 (3) ◽  
pp. 279-283 ◽  
Author(s):  
Ken-Ichi Tanno ◽  
Ayaka Takeuchi ◽  
Eri Akahori ◽  
Keiko Kobayashi ◽  
Taihachi Kawahara ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Marker ◽  
Pcr Amplification ◽  
Triticum Dicoccoides ◽  
Positive Control ◽  
Triticum Timopheevii ◽  
Pcr Marker ◽  
Simple Sequence ◽  
Triticum Araraticum ◽  
Complete Accuracy

AbstractWe developed a multiplex PCR DNA marker for quick and easy identification of the AAGG-genome timopheevii lineage, including Triticum timopheevii, Triticum araraticum and hexaploid Triticum zhukovskyi (AAAmAmGG), and the AABB-genome emmer wheat lineage, including Triticum durum, Triticum dicoccum and Triticum dicoccoides. Distinguishing between tetraploid AAGG- and AABB-genome wheat species based on morphology is known to be difficult. This multiplex PCR system is based on the simultaneous PCR amplification of two chloroplast regions, matK and rbcL. The matK region molecularly distinguishes the two lineages, whereas the rbcL region is a positive control amplicon. We also examined whether the simple sequence repeat is a fixed mutation within species, using genetic resources in the collection of KOMUGI, Kyoto University, which comprises accessioned species collected across diverse geographical areas. The multiplex PCR marker distinguished AAGG from AABB species with complete accuracy.

Development and implementation of a sequence-specific PCR marker linked to a gene conferring resistance to anthracnose disease in narrow-leafed lupin (Lupinus angustifolius L.)

2004 ◽  
Vol 14 (2) ◽  
pp. 145-151 ◽  
Author(s):  
Huaan Yang ◽  
Jeffery G. Boersma ◽  
Mingpei You ◽  
Bevan J. Buirchell ◽  
Mark W. Sweetingham
Keyword(s):  
Lupinus Angustifolius ◽  
Pcr Marker ◽  
Anthracnose Disease ◽  
Specific Pcr

scholarly journals INDEL variation in the regulatory region of the major flowering time gene LanFTc1 is associated with vernalization response and flowering time in narrow-leafed lupin (Lupinus angustifolius L.)

2018 ◽  
Vol 42 (1) ◽  
pp. 174-187 ◽  
Author(s):  
Candy M. Taylor ◽  
Lars G. Kamphuis ◽  
Weilu Zhang ◽  
Gagan Garg ◽  
Jens D. Berger ◽  
...  
Keyword(s):  
Flowering Time ◽  
Regulatory Region ◽  
Lupinus Angustifolius ◽  
Vernalization Response ◽  
Flowering Time Gene ◽  
Indel Variation

Development of a PCR marker tightly linked to mollis, the gene that controls seed dormancy in Lupinus angustifolius L.

2007 ◽  
Vol 126 (6) ◽  
pp. 612-616 ◽  
Author(s):  
J. G. Boersma ◽  
B. J. Buirchell ◽  
K. Sivasithamparam ◽  
H. Yang
Keyword(s):  
Seed Dormancy ◽  
Lupinus Angustifolius ◽  
Pcr Marker

Effect of genotype on yield of narrow-leafed lupin (Lupinus angustifolius) and sandplain lupin (Lupinus cosentinii)

1974 ◽  
Vol 14 (71) ◽  
pp. 742 ◽  
Author(s):  
P Farrington ◽  
JS Gladstones
Keyword(s):  
Flowering Time ◽  
Seed Yield ◽  
Vegetative Growth ◽  
Dry Matter ◽  
Flower Colour ◽  
Lupinus Angustifolius ◽  
Dry Matter Yield ◽  
Seed Colour ◽  
Developing Seeds ◽  
Matter Production

Dry matter production, pod set and seed yield were compared among five genotypes of Lupinus angustifolius and six genotypes of L. cosentinii with reduced or non-shattering pods. The genotypes differed in flowering time, alkaloid content and flower colour (L. angustifolius only). Presence of the low alkaloid gene iucundus in L. angustifolius reduced vegetative growth by 25 per cent, and did not significantly reduce seed yield. Susceptibility to 'split' seed, a disorder causing rupture of the testa of the developing seeds was also associated with the iucundus gene. The implications of this are discussed. Flower colour and maturity differences did not significantly affect yield, although there was some suggestion that the white flower and seed colour gene leucospermus reduced pod set and seed yield in earlyflowering genotypes. In L. cosentinii, the presence of the low alkaloid gene sw generally did not significantly affect dry matter or seed yield. An exception was in the very early flowering genotypes where dry matter yield was 23 per cent lower in the low alkaloid line.

A multiplex PCR-SSCP test to genotype bovine β-casein alleles A1 , A2 , A3 , B, and C

1999 ◽  
Vol 30 (4) ◽  
pp. 322-323 ◽  
Author(s):  
A. Barroso ◽  
S. Dunner ◽  
J. Ca·ón
Keyword(s):  
Multiplex Pcr

Multiplex PCR zum Nachweis disseminierter Tumorzellen (DTC) im Blut von Patientinnen mit Mammakarzinom

2005 ◽  
Vol 127 (03) ◽  
Author(s):  
J Rom ◽  
A Schneeeweiss ◽  
V Zieglschmid ◽  
C Hollmann ◽  
O Böcher ◽  
...  
Keyword(s):  
Multiplex Pcr
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