Role of beneficial microbes with nitrogen and phosphorous levels on canola productivity

A research was conducted to evaluate the impact of various nitrogen and phosphorus levels along with beneficial microbes to enhance canola productivity. The research was carried out at Agronomy Research Farm, The University of Agriculture Peshawar in winter 2016-2017. The experiment was conducted in randomized complete block factorial design. The study was comprised of three factors including nitrogen (60, 120 and 180 kg ha-1), phosphorous (70, 100 and 130 kg ha-1) and beneficial microbes (with and without BM). A control treatment with no N, P and BM was also kept for comparison. Application of beneficial microbes significantly increased pods plant, seed pod, seed filling duration, 1000 seed weight, biological yield and seed yield as compared to control plots. Nitrogen applied at the rate of 180 kg ha-1 increased pods plant-1, seed pod, seed filling duration, seed weight, biological yield and seed yield. Maximum pods plant-1, seed pod, early seed filling, heavier seed weight, biological yield, seed yield, and harvest index were observed in plots treated with 130 kg.ha-1 phosphorous. As comparison, the combine treated plots have more pods plant-1, seeds pod-1, seed filling duration, heaviest seeds, biological yield, seed yield and harvest index as compared to control plots. It is concluded that application of beneficial microbes with N and P at the rate of 180 kg ha-1 and 130 kg ha-1, respectively, increased yield and its attributes for canola.

EFFECT OF SEED FILLING AND RELEASING ANGLES ON THE PERFORMANCE OF COMPOUND VACUUM SEED METERING DEVICE

In this paper, taking compound air-suction seed metering device as the subject, the effect rule of seed filling angle and seed releasing angle on the performance of seed filling and seed metering is studied. An indoor test was conducted with a JPS-12 metering test bench and a high-speed camera system. The starting and ending angles of the air chamber were changed by adjusting the regulating plate, that is, the angles of seed filling and seed releasing, and the filling and seed metering performance was taken as evaluation indexes. The test results show that: the seed filling angle has significant effect on the seed filling effect, and the miss fill index decreases with the increase of the quality of fill index. When the seed filling angle exceeds 50°, they will stabilize gradually ending at the multiple fill index of 0%. The negative pressure and operating speed are interactive and have a significant effect on filling performance. The seed releasing angle has significant effect on the metering performance. With the increase of the angle, the quality of feed index increases firstly and then decreases. The multiple index and miss index are opposite to the quality of feed index which is the max at the seed releasing angle of 39.5°. The negative pressure and forward speed are interactive and have a significant effect on metering performance. The quality of feed index under the same condition is lower than that of fill index.

WORKSPACE ANALYSIS OF A FLAME INTRA-ROW WEEDING ROBOT IN VEGETABLE FIELD AND NUMERICAL SIMULATION OF PROPANE COMBUSTION

In order to improve the seed filling performance of mechanical hole type seed metering device with high speed, a vertical disc metering device was designed with seed agitator to improve the seed filling probability. Discrete element simulation method and physical prototype bench tests were used to simulate and analyse the seed metering process. The structure parameters of the seed agitators were optimized by the quadratic orthogonal rotation central composite design test. The results showed that: 1) the addition of seed agitator had a significant influence on the MIS (miss index), among which the concave type had the lowest MIS, and it was the highest without agitator; 2) for concave type agitator, the optimal value of depth and angle were 3.1 mm and 60.5°, respectively; 3) the physical prototype tests showed that the QFI (quality of feed index) was more than 95% under the speed of 2-10 km/h, and the MIS was less than 1%, which meets the requirements of precision sowing.

Defisit Air Pada Berbagai Fase Pertumbuhan dan Pengaruhnya Terhadap Karakter Kuantitatif Beberapa Genotipe Kacang Tanah

This study aims to determine the effect of water deficit at various phases of plant growth on the quantitative characters of several peanut genotypes. This study used a completely randomized design-split plot design. The water deficit consisted of 6 treatments: d0 = no water deficit, d1 = water deficit from germination to harvest, d2 = water deficit from germination to age 25 days after planting (dap) (vegetative phase), d3 = water deficit from age 26 to 50 dap (flowering phase to pod formation), d4 = water deficit from age 51 dap to 75 dap (seed filling phase), and d5 = water deficit from age 75 dap to 100 dap (seed ripening phase until harvest). The peanut genotype used consisted of 10 genotypes. The results showed that water deficit in various phases of plant growth resulted in different quantitative characters in several peanut genotypes. Genotype G3T4 produced heaviest dry pod weight of 12.7 g plant-1 in water deficit from germination to harvest. Genotype G200-I produced heaviest dry pod weight of 11.5 g per plant-1 in water deficit in the vegetative phase. Genotype G3T4 produced heaviest dry pod weight of 13.3 g per plant in water deficit the generative phase. Genotype G300-II produced heaviest the dry pod weight of 11.7 g per plant-1 in the water deficit of the seed filling phase. Genotypes G2D2, G2T3 and G200-I produced the heaviest dry pod weight of 11.0 g per plant-1 in the water deficit of the seed ripening phase.

Differential Assembly and Shifts of the Rhizosphere Bacterial Community by a Dual Transgenic Glyphosate-Tolerant Soybean Line with and without Glyphosate Application

Modern agriculture has gained significant economic benefits worldwide with the use of genetically modified (GM) technologies. While GM crops provide convenience to humans, their biosafety has attracted increasing concern. In this study, the Illumina MiSeq was used to perform a high-throughput sequencing of the V3-V4 hypervariable regions of 16S rRNA gene (16S rDNA) amplicons to compare the rhizosphere bacterial communities of the EPSPS/GAT dual transgenic glyphosate-tolerant soybean line Z106, its recipient variety ZH10, and Z106 with glyphosate application (Z106G) during flowering, seed filling, and maturing stages under field settings. At each of the three stages, the alpha and beta diversity of rhizosphere bacterial communities revealed no significant differences between ZH10, Z106, and Z106G. However, some bacterial taxa demonstrated a greater proportional contribution, particularly the nitrogen-fixing rhizobium Ensifer fredii, in the rhizospheric soil of Z106 at the seed filling and maturing stages, when compared to ZH10 and Z106G. The present study therefore suggests that the EPSPS/GAT dual transgenic line Z106 and exogenous glyphosate application have a minimal effect on the composition of the soybean rhizosphere bacterial community but have no impact on the structure of the rhizosphere microbial community during a single planting season.

Evaluation of Mungbean Genotypes for Phenophases and Morpho-physiological Traits during Heat and Water Stress Conditions for Optimizing Productivity

An experiment was carried out to study the effect of phenophases, morpho-physiological parameters and yield attributing traits on mungbean under different dates of sowing during summer seasons of 2017-18 and 2018-19 at the Research area, Department of Plant Physiology, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur (Madhya Pradesh). The experiment was laid out in a factorial randomized block design replicated thrice. Treatments consisted of three sowing environments viz., Feb 12th, Feb 27th and Mar 14th and five mungbean varieties viz., PDM 139, Pusa Ratna, Pusa Vishal, Pusa 1431 and TJM 3. PDM139 (V1) had an earliest attainment of all the phenophases and registered the lowest time for reproductive period (32.00 days) as well as span of seed filling period (18.66 days) and also recorded average highest LAI (0.723), LAD (7717.48 cm2. days), CGR (0.00125 g cm2 day-1), RGR (0.03614 g g-1 day-1), SLA (391.94 cm2 g-1) and carbon sequestration (58.93 g plant-1). PDM139 (V1) outyielded (5.78 g plant-1 and 787.74 Kg ha-1) others owing higher magnitudes of physiological parameters and mechanisms reflected in maximum yield components and subsequently yield. On the other hand D2 (27th Feb.) took comparatively more time to achieve all the phenophase as compared to the former as well as average highest magnitudes of these parameters. Among sowing dates sowing carried out on 14th Mar (D3) acquired the minimum time to attain all the phenophases. Among interactions, V1D2 (PDM139 sown on 27th Feb.) took comparatively more time to attain flower initiation (26.33 days) besides a short duration of reproductive span (31.00 days) and a comparatively low magnitudes of seed filling period (18.00 days) and as well as recorded average maximum magnitudes of LAI (Leaf Area Index), LAD (Leaf Area Duration, CGR (Crop Growth Rate), RGR (Relative Growth Rate), SLA (Specific Leaf Area), SLW (Specfic Leaf Weight) and carbon sequestration. Therefore, based on above results selection of genotypes could bring out desired improvement in yield and its attributing characters of mungbean cultivars.

Combining Ability And Nature of Gene Effects Controlling Seed Yield And Its Component Traits In Alien Cytoplasm Based Hybrids In Sunflower (Helianthus Annuus L.)

The present research aimed to study gene effects for seed yield and its component traits in sunflower hybrids having diverse cytosterile sources from Helianthus annuus and H. argophyllus. Five lines and ten testers were crossed in a line x tester mating design to develop 50 F1 hybrids for genetic analysis. The analysis of variance revealed significant differences among the traits studied. Seasonal variation was found significant for all the traits except stem diameter, volume weight and seed filling percentage. The mean squares of lines and testers from crosses both determine the GCA were also significant for most of the traits which revealed the prevalence of additive variances and additive gene action. The mean squares of lines x tester interactions were also significant for all the traits considered. The significance of lines x tester interactions indicated that SCA is also important in the expression of traits and demonstrated the value of non-additive variances and dominant genes controlling the various traits. Among the diverse cytosterile lines, ARG-6-3-1-4 was identified as the best general combiner for stem diameter, volume weight, seed yield, hull content, oil content and oil yield. While, the line ARG-2-1-2 was best general combiner for days to 50 per cent flowering, head diameter, 100 seed weight and seed filling percentage. The tester M17-R was observed to be best general combiner for earliness and volume weight, while RHA 93 was the best general combiner for plant height, seed yield and oil content. The best cross combinations; MUT-2-8-3-2 x GKVK 3 was found to be good specific combiner for stem diameter, 100 seed weight, seed yield, seed filling percentage, oil content and oil yield, while ARG-6-3-1-4 x GKVK 3 was good specific combiner for days to 50% flowering, plant height and 100 seed weight.

Seed dormancy of Lolium perenne L. related to the maternal environment during seed filling

Lolium perenne L. (perennial ryegrass) shows variable levels of seed physiological dormancy (PD), which depends on the genotype and environmental condition during seed development. To analyse the effect of field temperature and precipitation during seed filling on the PD, two cultivars were sown on five dates in 2014 and 2015. After harvest, the level of seed PD was 4–28%. High-temperature stress (>29°C) in the field during seed development, measured as heat stress units (HSUs), reduced seed PD (increased germination) at harvest. After 9 months of dry afterripening under laboratory conditions, mean dormant seed values were reduced from 15 ± 8 to 8 ± 7%. An increment in the seed PD level reduced seedling emergence in the field. Seed with 20% PD produced only 50% of field emergence, under optimal environmental conditions. Different vigour tests were conducted and each was compared with field emergence. The speed of germination, through the first count at 5 d of the standard germination test, and the shoot length at 10 d were better associated with the seedling establishment in the field. The HSU could be useful to establish a possible PD range in the seed of perennial ryegrass after the growing season. The development of models considering the HSU and other climatic parameters could motivate future studies.