ADH2/GSNOR1 is a key player in limiting genotoxic damage mediated by formaldehyde and UV‐B in Arabidopsis

2021 ◽  
Author(s):  
Yu Wang ◽  
Jinzheng Wang ◽  
Qiang Lv ◽  
Yi‐Kun He
Keyword(s):  
Genotoxic Damage

Nuclear distribution of genotoxic damage in rainbow trout (Oncorhynchus mykiss) sperm after cryopreservation

2014 ◽  
Author(s):  
Silvia Gonzalez-Rojo ◽  
Cristina Fernandez-Diez ◽  
Marta Lombo ◽  
Vanesa Robles Rodriguez ◽  
Herraez Maria Paz
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Genotoxic Damage ◽  
Nuclear Distribution ◽  
Rainbow Trout Oncorhynchus Mykiss

Evaluation of radiation-induced genotoxicity on hu-man melanoma cells (SK-MEL-37) by flow cytometry

2019 ◽  
Vol 7 (2A) ◽  
Author(s):  
Leticia Bonfim ◽  
Luma Ramirez de Carvalho ◽  
Daniel Perez Vieira
Keyword(s):  
Genetic Material ◽  
Micronucleus Assay ◽  
Melanoma Cells ◽  
Dead Cell ◽  
Genotoxic Damage ◽  
Sytox Green ◽  
60Co Source ◽  
Radiation Induced ◽  
Death And Loss ◽  
Cell Fractions

Micronucleus assay is a test used to evaluate genotoxic damage in cells, which can be caused by various factors, like ionizing radiation. Interactions between radiation energies and DNA can cause breakage, leading to use chromosomal mutations or loss of genetic material, important events that could be induced in solid tumors to mitigate its expansion within human body.  Melanoma has been described as a tumor with increased radio resistance.  This work evaluated micronuclei percentages (%MN) in human melanoma cells (SK-MEL-37), irradiated by gamma radiation, with doses between 0 and 16Gy.  Cell suspensions were irradiated in PBS by a 60Co source in doses between 0 and 16Gy, and incubated by 48h.  Then cell membranes were lysed in the presence of SYTOX Green and EMA dyes, preserving nuclear membranes.  Using this method, EMA-stained nuclei could be discriminated as those derived from dead cells, and SYTOX nuclei and micronuclei could be quantified.  Micronuclei percentages were found to be proportional to dose, (R2 = 0.997).  Only the highest dose (16Gy) could induce statistically significant increase of MN (p<0.0001), although cultures irradiated by 4, 8 and 16Gy showed significant increase of dead cell fractions.  Calculation of the nuclei-to-beads ratio showed that 8 and 16Gy could reduce melanoma cell proliferation.  Results showed that although cell death and loss of proliferative capacity could be observed on cultures irradiated at lower doses, genotoxic damage could be induced only on a higher dose. Resistance to radiation-induced genotoxicity could explain a relatively high radio resistance of melanoma tumors.

GSTT2, a phase II gene induced by apple polyphenols, protects colon epithelial cells against genotoxic damage

2009 ◽  
Vol 53 (10) ◽  
pp. 1245-1253 ◽  
Author(s):  
Astrid Petermann ◽  
Claudia Miene ◽  
Gabriele Schulz-Raffelt ◽  
Katja Palige ◽  
Jana Hölzer ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Phase Ii ◽  
Genotoxic Damage

The use of micronucleus test for detection of genotoxic damage to the thyroid gland

2006 ◽  
Vol 141 (1) ◽  
pp. 91-94 ◽  
Author(s):  
A. V. Pavlov ◽  
M. A. Gansburgskii ◽  
A. N. Gansburgskii ◽  
M. V. Shashkina ◽  
T. L. Miro
Keyword(s):  
Thyroid Gland ◽  
Micronucleus Test ◽  
Genotoxic Damage

scholarly journals Genotoxic biomarkers in patients on hemodialiasis

2020 ◽  
Vol 18 (3) ◽  
pp. 367-389
Author(s):  
Natalia V. Eremina ◽  
Andrey D. Durnev
Keyword(s):  
Peripheral Blood ◽  
Human Life ◽  
Peripheral Blood Lymphocytes ◽  
Chromosome Damage ◽  
Literature Information ◽  
Genotoxic Effects ◽  
Blood Lymphocytes ◽  
Genotoxic Damage ◽  
Important Measure

It is generally recognized that genotoxic damage have essential etiopathogenetic significance, and its prevention is an important measure to preserve human life and health. In the framework of this concept, literature information on studies of genotoxic biomarkers in patients with various hemodialysis regiments has been reviewed and summarized, and ways to prevent detectable genotoxicity have been identified. Based on the analysis of the known data, it was concluded that patients of this group have an increased level of DNA and chromosome damage in peripheral blood lymphocytes. Based on the results of individual studies, it was shown that one of the strategies for reducing genotoxicity may be the improvement of hemodialysis therapy methods and regimes, as well as pharmacological and nutritional correction of genotoxic effects.

scholarly journals Amelioration of the cyclophosphamide induced genotoxic damage in mice by the ethanolic extract of Equisetum arvense

2017 ◽  
Vol 4 ◽  
pp. 226-233 ◽  
Author(s):  
Jasbir Kour ◽  
Md Niamat Ali ◽  
Hilal Ahmad Ganaie ◽  
Nahida Tabassum
Keyword(s):  
Ethanolic Extract ◽  
Equisetum Arvense ◽  
Genotoxic Damage

Genotoxic damage in end-stage renal disease

2018 ◽  
Vol 835 ◽  
pp. 1-10 ◽  
Author(s):  
G. Gandhi ◽  
T. Mehta ◽  
P. Contractor ◽  
G. Tung
Keyword(s):  
Renal Disease ◽  
End Stage Renal Disease ◽  
Genotoxic Damage ◽  
Stage Renal Disease ◽  
End Stage
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