Exposure of Human Skin Models to KrCl Excimer Lamps: The Impact of Optical Filtering †

In contemporary bioanalysis, monitoring the antioxidant activity (AOA) of the human skin is used to assess stresses, nutrition, cosmetics, and certain skin diseases. Non-invasive methods for skin AOA monitoring have certain advantages over invasive methods, namely cost-effectiveness, lower labor intensity, reduced risk of infection, and obtaining results in the real-time mode. This study presents a new flexible potentiometric sensor system (FPSS) for non-invasive determination of the human skin AOA, which is based on flexible film electrodes (FFEs) and membrane containing a mediator ([Fe(CN)6]3–/4–). Low-cost available materials and scalable technologies were used for FFEs manufacturing. The indicator FFE was fabricated based on polyethylene terephthalate (PET) film and carbon veil (CV) by single-sided hot lamination. The reference FFE was fabricated based on PET film and silver paint by using screen printing, which was followed by the electrodeposition of precipitate containing a mixture of silver chloride and silver ferricyanide (SCSF). The three-electrode configuration of the FPSS, including two indicator FFEs (CV/PET) and one reference FFE (SCSF/Ag/PET), has been successfully used for measuring the skin AOA and evaluating the impact of phytocosmetic products. FPSS provides reproducible (RSD ≤ 7%) and accurate (recovery of antioxidants is almost 100%) results, which allows forecasting its broad applicability in human skin AOA monitoring as well as for evaluating the effectiveness of topically and orally applied antioxidants.

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Evaluation of in vitro human skin models for studying effects of external stressors and stimuli and developing treatment modalities

View ◽

10.1002/viw.20210012 ◽

2021 ◽

pp. 20210012

Author(s):

Emily Sutterby ◽

Peter Thurgood ◽

Sara Baratchi ◽

Khashayar Khoshmanesh ◽

Elena Pirogova

Keyword(s):

Human Skin ◽

Treatment Modalities ◽

Human Skin Models

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The Impact of Environmental and Endogenous Damage on Somatic Mutation Load in Human Skin Fibroblasts

PLoS Genetics ◽

10.1371/journal.pgen.1006385 ◽

2016 ◽

Vol 12 (10) ◽

pp. e1006385 ◽

Cited By ~ 48

Author(s):

Natalie Saini ◽

Steven A. Roberts ◽

Leszek J. Klimczak ◽

Kin Chan ◽

Sara A. Grimm ◽

...

Keyword(s):

Human Skin ◽

Somatic Mutation ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts ◽

Mutation Load ◽

The Impact

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Improved epidermal barrier formation in human skin models by chitosan modulated dermal matrices

PLoS ONE ◽

10.1371/journal.pone.0174478 ◽

2017 ◽

Vol 12 (3) ◽

pp. e0174478 ◽

Cited By ~ 12

Author(s):

Arnout Mieremet ◽

Marion Rietveld ◽

Samira Absalah ◽

Jeroen van Smeden ◽

Joke A. Bouwstra ◽

...

Keyword(s):

Human Skin ◽

Epidermal Barrier ◽

Barrier Formation ◽

Dermal Matrices ◽

Human Skin Models

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Comparison of cutaneous bioavailability of cosmetic preparations containing caffeine or -tocopherol applied on human skin models or human skin ex vivo at finite doses

The Essential Stratum Corneum ◽

10.1201/b14296-22 ◽

2002 ◽

pp. 125-134

Author(s):

F Dreher ◽

F Fouchard ◽

C Patouillet ◽

M Andrian ◽

J-T Simonnet ◽

...

Keyword(s):

Human Skin ◽

Ex Vivo ◽

Human Skin Models

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Interaction between SARS-CoV-2 spike glycoprotein and human skin models: a molecular dynamics study

10.1101/2021.07.13.452154 ◽

2021 ◽

Author(s):

Marc Domingo ◽

Jordi Faraudo

Keyword(s):

Stratum Corneum ◽

Human Skin ◽

Skin Surface ◽

Viral Envelope ◽

Solid Surfaces ◽

Spike Glycoprotein ◽

Indirect Transmission ◽

Physico Chemical ◽

Human Skin Models ◽

Structure Orientation

The possibility of contamination of human skin by infectious virions plays an important role in indirect transmission of respiratory viruses but little is known about the fundamental physico-chemical aspects of the virus-skin interactions. In the case of coronaviruses, the interaction with surfaces (including the skin surface) is mediated by their large glycoprotein spikes that protrude from (and cover) the viral envelope. Here, we perform all atomic simulations between the SARS-CoV-2 spike glycoprotein and human skin models. We consider an "oily" skin covered by sebum and a "clean" skin exposing the stratum corneum. The simulations show that the spike tries to maximize the contacts with stratum corneum lipids, particularly ceramides, with substantial hydrogen bonding. In the case of "oily" skin, the spike is able to retain its structure, orientation and hydration over sebum with little interaction with sebum components. Comparison of these results with our previous simulations of the interaction of SARS-CoV-2 spike with hydrophilic and hydrophobic solid surfaces, suggests that the"soft" or "hard" nature of the surface plays an essential role in the interaction of the spike protein with materials.

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Kinetic Cytokine Secretion Profile of LPS-Induced Inflammation in the Human Skin Organ Culture

Pharmaceutics ◽

10.3390/pharmaceutics12040299 ◽

2020 ◽

Vol 12 (4) ◽

pp. 299 ◽

Cited By ~ 1

Author(s):

Raanan Gvirtz ◽

Navit Ogen-Shtern ◽

Guy Cohen

Keyword(s):

Organ Culture ◽

Human Skin ◽

Comprehensive Evaluation ◽

Ex Vivo ◽

Cytokine Secretion ◽

Peak Time ◽

Local Skin ◽

The Impact ◽

Skin Organ Culture

Several in vitro models that mimic different aspects of local skin inflammation exist. The use of ex vivo human skin organ culture (HSOC) has been reported previously. However, comprehensive evaluation of the cytokine secretory capacity of the system and its kinetics has not been performed. Objective: the aim of the current study was to investigate the levels and secretion pattern of key cytokine from human skin tissue upon lipopolysaccharide (LPS) stimulation. HSOC maintained in an air–liquid interface was used. Epidermal and tissue viability was monitored by MTT and Lactate Dehydrogenase (LDH) activity assay, respectively. Cytokine levels were examined by ELISA and multiplex array. HSOCs were treated without or with three different LPS subtypes and the impact on IL-6 and IL-8 secretion was evaluated. The compounds enhanced the secreted levels of both cytokines. However, differences were observed in their efficacy and potency. Next, a kinetic multiplex analysis was performed on LPS-stimulated explants taken from three different donors to evaluate the cytokine secretion pattern during 0–72 h post-induction. The results revealed that the pro-inflammatory cytokines IL-6, IL-8, TNFα and IL-1β were up-regulated by LPS stimuli. IL-10, an anti-inflammatory cytokine, was also induced by LPS, but exhibited a different secretion pattern, peak time and maximal stimulation values. IL-1α and IL-15 showed donor-specific changes. Lastly, dexamethasone attenuated cytokine secretion in five independent repetitions, supporting the ability of the system to be used for drug screening. The collective results demonstrate that several cytokines can be used as valid inflammatory markers, regardless of changes in the secretion levels due to donor’s specific alterations.

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