Application of energy‐filtering electron microscopy (EFEM) for analysis of hydrogen peroxide and lignin in epidermal walls of cucumber leaves triggered by acibenzolar‐ S ‐methyl treatment prior to inoculation with Colletotrichum orbiculare

2019 ◽  
Vol 68 (9) ◽  
pp. 1624-1634 ◽  
Author(s):  
P. Park ◽  
T. Kurihara ◽  
M. Nita ◽  
K. Ikeda ◽  
K. Inoue ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Hydrogen Peroxide ◽  
Colletotrichum Orbiculare ◽  
Energy Filtering

The use of an energy-filtering electron microscope to reduce chromatic aberration in images of thick biological specimens

1986 ◽  
Vol 44 ◽  
pp. 88-91
Author(s):  
L. D. Peachey ◽  
J. P. Heath ◽  
G. Lamprecht
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Beam ◽  
Cross Section ◽  
Electron Scattering ◽  
Chromatic Aberration ◽  
Image Resolution ◽  
Incident Electron Energy ◽  
Energy Filtering ◽  
Transmission Electron

Biological specimens of cells and tissues generally are considerably thicker than ideal for high resolution transmission electron microscopy. Actual image resolution achieved is limited by chromatic aberration in the image forming electron lenses combined with significant energy loss in the electron beam due to inelastic scattering in the specimen. Increased accelerating voltages (HVEM, IVEM) have been used to reduce the adverse effects of chromatic aberration by decreasing the electron scattering cross-section of the elements in the specimen and by increasing the incident electron energy.

scholarly journals Development of a New Acquisition Scheme for Energy-Filtering Transmission Electron Microscopy Spectrum-Imaging toward Quantification

2011 ◽  
Vol 17 (S2) ◽  
pp. 790-791
Author(s):  
M Watanabe ◽  
F Allen
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Energy Filtering ◽  
Transmission Electron ◽  
Acquisition Scheme ◽  
Spectrum Imaging

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

Three-Dimensional Holey-Graphene Architectures for Highly Sensitive Enzymatic Electrochemical Determination of Hydrogen Peroxide

2019 ◽  
Vol 19 (11) ◽  
pp. 7404-7409 ◽  
Author(s):  
Aihua Jing ◽  
Gaofeng Liang ◽  
Hao Shi ◽  
Yixin Yuan ◽  
Quanxing Zhan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Hydrogen Peroxide ◽  
Graphene Oxide ◽  
Three Dimensional ◽  
Electrochemical Determination ◽  
High Specific Surface Area ◽  
3D Graphene ◽  
Practical Applications ◽  
Transmission Electron ◽  
Mild Defect

Three-dimensional (3D) graphene with high specific surface area, excellent conductivity and designed porosity is essential for many practical applications. Herein, holey graphene oxide with nano pores was facilely prepared via a convenient mild defect-etching reaction and then fabricated to 3D nanostructures via a reduction method. Based on the 3D architectures, a novel enzymatic hydrogen peroxide sensor was successfully fabricated. Transmission electron microscopy (TEM), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) were used to characterize the 3D holey graphene oxide architectures (3DHGO). Cyclic voltammetry (CV) was used to evaluate the electrochemical performance of 3DHGO at glassy carbon electrode (GCE). Excellent electrocatalytic activity to the reduction of H2O2 was observed, and a linear range of 5.0×10-8~5.0×10-5 M with a detection limit of 3.8×10-9 M was obtained. These results indicated that 3DHGO have potential as electrochemical biosensors.

Energy filtering transmission electron microscopy using the new JEM-2010FEF

1999 ◽  
Vol 194 (1) ◽  
pp. 210-218 ◽  
Author(s):  
TOMOKIYO ◽  
MATSUMURA ◽  
MANABE
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Energy Filtering ◽  
Transmission Electron

scholarly journals Analysis of Metal/Plastic Interfaces by Energy-Filtering Transmission Electron Microscopy

2012 ◽  
Vol 48 (9) ◽  
pp. 322-330 ◽  
Author(s):  
Shin HORIUTI ◽  
Takeshi HANADA ◽  
Takayuki MIYAMAE ◽  
Tadae YAMANAKA ◽  
Kogoro OOSUMI ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Energy Filtering ◽  
Transmission Electron

Development of New Marker Compounds for the Detection of Chemical Element Labels by Electron Spectroscopic Imaging (ESI)

2001 ◽  
Vol 7 (S2) ◽  
pp. 1038-1039
Author(s):  
S. Raddatz ◽  
E. P. Mark ◽  
A. Haking ◽  
W. Probst ◽  
M. Wiessler ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Chemical Element ◽  
Three Dimensional ◽  
Point Of View ◽  
High Concentration ◽  
Specific Chemical ◽  
Energy Filtering ◽  
Transmission Electron ◽  
Gold Particle Size ◽  
Marker Compounds

A promising aspect of ESI is its application in the detection of elemental labels introduced into biomolecules for cell and molecular biological techniques. Even though colloidal gold labeling for electron microscopy (EM) is highly developed, availability of alternative labels, especially for double or triple labeling applications would be helpful because of difficulties with gold concerning i) detection (gold diameters ≤1nm), ii) discrimination due to gold particle size variations in one size class, and iii) different labeling efficiencies depending on gold granule size. An alternative labeling molecule should contain a high concentration of a specific chemical element which is not or in minor concentrations present in the system under surveillance, and has to have the potential to be discriminated from “biological” elements by ESI.With respect to ESI, one candidate for elemental labeling is boron. It meets the criteria described above and substantial experience in the synthesis of labeling compounds exists. From the chemical point of view, the preferred labeling structure is a so called dendrimer, a highly branched regular three-dimensional monodisperse macromolecule. Dendritic structures offer a large variety of functionalities to incorporate an element detectable by energy filtering transmission electron microscopy (EFTEM).

Pre-Martensitic State in a Ti50Ni48Fe2 Alloy Studied by Electron Microscopy with Energy-Filtering

1999 ◽  
Vol 5 (S2) ◽  
pp. 874-875
Author(s):  
Y. Murakami ◽  
Y. Ikematsu ◽  
D. Shindo ◽  
T. Oikawa ◽  
M. Kersker
Keyword(s):  
Electron Microscopy ◽  
Diffuse Scattering ◽  
Parent Phase ◽  
Morphological Aspect ◽  
Energy Filtering ◽  
Precise Analysis ◽  
Martensitic State ◽  
Typical Shape ◽  
Diffraction Patterns ◽  
Type Magnet

A Ti50Ni48Fe2 alloy, which is famous as a typical shape memory alloy, exhibits the cubic (parent phase) to trigonal (R-phase) martensitic transformation upon cooling. It is well known that this structural transformation is accompanied by some precursor phenomena, such as appearance of weak diffuse scattering in electron diffraction patterns of the parent phase. Since the diffuse scattering is visible at around 1/31/3 0 and its equivalent positions in diffraction patterns, as similar to the superlattice reflections in the R-phase, it is believed to be closely related with the formation of the R-phase. However, neither structural nor morphological aspect of this pre-martensitic state is clear, since a precise analysis of the weak diffuse scattering is quite difficult along with a conventional electron microscopy, because of the presence of strong background arising from inelastic scattering. The purpose of the present work is to precisely observe the weak diffuse scattering and to reveal the structural and morphological features of the pre-martensitic state by electron microscopy with the aid of a recently developed omega-type magnet.

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