Endometrial biopsy in Bos indicus beef heifers

2017 ◽  
Vol 52 (3) ◽  
pp. 526-528
Author(s):  
O Ramirez-Garzon ◽  
N Satake ◽  
RE Lyons ◽  
J Hill ◽  
MK Holland ◽  
...  
Keyword(s):  
Bos Indicus ◽  
Endometrial Biopsy ◽  
Beef Heifers

PSV-4 Peri-estrus ovarian, uterine, and hormonal variables influence the uterine luminal fluid metabolome in beef heifers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 309-310
Author(s):  
Felipe Alves Correa Carvalho da Silva ◽  
Gabriela da Silva ◽  
Bruna Vieira ◽  
Adomar Neto ◽  
Cecilia Constantino Rocha ◽  
...  
Keyword(s):  
Color Doppler ◽  
Univariate Analysis ◽  
Bos Indicus ◽  
Essential Amino Acids ◽  
Beef Heifers ◽  
Spatial Changes ◽  
Temporal And Spatial ◽  
Cytology Brush ◽  
Implantation Period ◽  
Uterine Body

Abstract In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Overarching hypothesis was that peri-estrus changes in ULF volume through accumulation and resorption mechanisms influence ULF composition during the estrous cycle and early pregnancy. Objectives were (1) to characterize individual temporal and spatial changes in ULF volume, endometrial and luteal vascularity, endometrial and luteal size, and progesterone (P4) concentrations during the peri-estrus period in beef heifers and, (2) associate such changes with the metabolite composition in the ULF, four days after estrus. Fourteen Bos indicus heifers that presented a PGF2α responsive CL received 500 µg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (estrus = d 0). Plasma P4 was measured daily. On d 4, the uterine body was sampled using a cytology brush for targeted metabolomic analysis by mass spectrometry. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Individual metabolite concentrations were compared between clusters A and B by univariate analysis using t-test after FDR correction. Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of non-essential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and associated uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers, which was associated with the ULF composition four days after estrus. Potential implications for embryo receptivity and reproductive outcomes are the focus of the current investigation.

1274 Pre-weaning injections of bovine somatotropin enhanced puberty attainment of bos indicus-influenced beef heifers

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 615-615
Author(s):  
G. M. Silva ◽  
P. Moriel ◽  
J. M. B. Vendramini ◽  
J. D. Arthington
Keyword(s):  
Bos Indicus ◽  
Bovine Somatotropin ◽  
Beef Heifers

39 EFFECT OF TIME OF FIXED-TIME AI ON PREGNANCY RATES IN ZEBU CROSS-BRED BEEF HEIFERS TREATED WITH PROGESTERONE RELEASING DEVICES AND ESTRADIOL CYPIONATE

2010 ◽  
Vol 22 (1) ◽  
pp. 177 ◽  
Author(s):  
M. Ramos ◽  
L. Cutaia ◽  
P. Chesta ◽  
G. A. Bó
Keyword(s):  
Bos Taurus ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Pregnancy Rates ◽  
Beef Heifers ◽  
Treatment Groups ◽  
Condition Score ◽  
To Receive

Two experiments were designed to evaluate the effect of the timing of fixed-time AI (FTAI) in relation to the removal of an intravaginal progesterone-releasing device (1 g of progesterone, DIB, Syntex SA, Buenos Aires, Argentina) on pregnancy rates in Bos indicus × Bos taurus cross-bred heifers. In experiment 1, 285 Bonsmara × zebu cross-bred heifers, between 18 and 24 months of age and with a body condition score (BCS) between 3.0 and 3.5 (1-5 scale) were used. On the day of initiation of treatment (Day 0), the heifers’ ovaries were palpated (92% of them had a CL) and they received a new DIB plus 2 mg of estradiol benzoate (EB; Syntex SA) and 250 μg of cloprostenol (Ciclase DL, Syntex SA). On Day 8, DIB devices were removed and all heifers received 250 μg of Ciclase plus 0.5 mg of estradiol cypionate (ECP; Cipiosyn, Syntex SA). At that time the heifers were randomly divided to receive FTAI between 48 to 49 h, 53 to 54 h, or 58 to 59 h after DIB removal. The heifers underwent FTAI with semen from 4 bulls by 2 inseminators. In experiment 2, 260 heifers from the same group as those used in experiment 1 (87% with a CL) were treated exactly as those in experiment 1, except that previously used DIB was inserted on Day 0. Pregnancy diagnosis was performed 30 days post-fixed-time AI by ultrasonography. The data were analyzed by logistic regression, taking into account the effect of time of FTAI, semen, and inseminator on pregnancy rates. In experiment 1, pregnancy rates were lower (P = 0.04) in the heifers undergoing FTAI between 48 and 49 h after DIB removal (46/95, 48.4%) than those undergoing FTAI 53 to 54 h (61/99, 61.6%) or 58 to 60 h (57/91, 62.6%) after DIB removal. However, no differences in pregnancy rates were found (P = 0.72) in experiment 2 between the 3 treatment groups, with 39/91 (42.9%) for the 48 to 49 h group, 45/89 (50.6%) for the 53 to 54 h group, and 35/89 (43.8%) for the 58 to 59 h group. There was no effect of the semen or inseminator (P > 0.2) in either experiment. We conclude that when Bos indicus × Bos taurus beef heifers are synchronized with new DIB devices and ECP, higher pregnancy rates are obtained in heifers undergoing FTAI late (between 53 to 60 h after DIB removal) than in those undergoing FTAI early (48 to 49 h after DIB removal). However, time of insemination does not apparently affect pregnancy rates when Bos indicus × Bos taurus beef heifers are synchronized with previously used DIB devices and ECP.

scholarly journals 119 Effects of temperament on growth, plasma cortisol concentrations, and puberty attainment in Bos indicus beef heifers.

2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 371-372
Author(s):  
R Cooke ◽  
P Moriel ◽  
B Cappellozza ◽  
J Moraes Vasconcelos
Keyword(s):  
Plasma Cortisol ◽  
Bos Indicus ◽  
Beef Heifers

Evaluation of the impacts of spaying by either the dropped ovary technique or ovariectomy via flank laparotomy on the welfare of Bos indicus beef heifers and cows1

2013 ◽  
Vol 91 (1) ◽  
pp. 382-394 ◽  
Author(s):  
J. C. Petherick ◽  
K. McCosker ◽  
D. G. Mayer ◽  
P. Letchford ◽  
M. McGowan
Keyword(s):  
Bos Indicus ◽  
Beef Heifers

scholarly journals 22 Puberty induction protocol, but not supplement amount, overcomes the negative impacts of reduced frequency of supplementation on reproduction of beef heifers

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 17-17
Author(s):  
Juliana Ranches ◽  
Marcelo Vedovatto ◽  
Elizabeth Palmer ◽  
Miguel Miranda ◽  
John Arthington ◽  
...  
Keyword(s):  
Factorial Design ◽  
Bos Indicus ◽  
Beef Heifers ◽  
Pregnancy Diagnosis ◽  
Frequency Rate ◽  
Reduced Frequency ◽  
Rate Effects ◽  
Supplementation Frequency ◽  
Negative Impacts

Abstract This study evaluated the performance of Bos indicus-influenced beef heifers submitted to a puberty induction protocol and offered two supplementation amounts and delivery frequencies. Sixty-four Brangus crossbred heifers were stratified by BW and age (244 ± 4 kg; 311 ± 18 d) and assigned into 1 of 16 bahiagrass pastures on d 0. Treatments were randomly assigned to pastures in a 2 × 2 factorial design (4 pastures/treatment) and consisted of soyhulls-based supplementation at 1.25% or 1.75% of BW (DM basis) offered daily or 3 times weekly for 168 d. The puberty induction protocol consisted of CIDR insert on d 56 and removal on d 70, 5-mL PGF2a injection on d 86, 2-mL GnRH injection and timed-AI on d 89. Heifers were exposed to Angus bulls from d 89 to 167 (1 bull/pasture). Pregnancy diagnosis was assessed on d 212. Frequency × rate effects were not detected (P ≥ 0.19) for any variable. Reducing the supplementation frequency from daily to 3X weekly tended (P = 0.07) to decrease overall ADG and puberty attainment on d 56 (53 vs. 41 ± 6%), but not puberty attainment on d 89 (P = 0.45; 84 vs. 78 ± 6%) and pregnancy percentage on d 212 (P = 0.97; 69 vs. 69 ± 7%). Increasing the supplement amount from 1.25% to 1.75% of BW increased (P = 0.04) overall ADG, tended (P = 0.09) to hasten puberty attainment on d 56 (40 vs. 54 ± 6%) and d 89 (74 vs. 88 ± 6%), and enhanced (P = 0.03) pregnancy percentage on d 212 (53 vs. 80 ± 7%). Therefore, the inclusion of a puberty induction protocol, but not increasing the supplement amount, enabled a reduced frequency of supplementation (3 vs. 7 times weekly) without detrimental impacts on percentage of pregnant yearling beef heifers.

510 Impact of estrus synchronization and fixed-time artificial insemination on calving distribution in Bos indicus influenced beef heifers

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 249-249
Author(s):  
N. Oosthuizen ◽  
P. L. P. Fontes ◽  
C. D. Sanford ◽  
F. M. Ciriaco ◽  
D. D. Henry ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Bos Indicus ◽  
Fixed Time ◽  
Estrus Synchronization ◽  
Beef Heifers

scholarly journals Considerations for reproductive management of bos indicus-influenced beef heifers

2017 ◽  
Author(s):  
◽  
James William Cameron Locke
Keyword(s):  
United States ◽  
Bos Taurus ◽  
Bos Indicus ◽  
Fixed Time ◽  
The United States ◽  
Pregnancy Rates ◽  
Breeding Period ◽  
Beef Heifers ◽  
Reproductive Management ◽  
Natural Service

The Bos indicus species of beef cattle presents a number of unique reproductive challenges, especially with regard to those that influence reproductive efficiency including timely attainment of puberty, response to certain pharmaceutical drugs used to control estrus and ovulation, and response to physiological stressors. Cattle of this biological type comprise a substantial percentage of the beef cattle population in southern regions of the United States because of their ability to withstand high ambient temperatures and relative humidity. Additionally, Bos indicus cattle are regarded to exhibit increased parasite and disease resistance, resulting in improved production in subtropical climates as compared to Bos taurus cattle in those same regions. Research utilizing composite breeds of Bos taurus and Bos indicus beef heifers has been limited in the United States, and adoption of reproductive technologies such as artificial insemination and estrus synchronization has been sluggish compared to Bos taurus cattle in other regions of the country. To help answer a number of questions about this biological type, an experiment was designed to evaluate reproductive performance of Bos indicus-influenced heifers (n = 1,456). Weights and reproductive tract scores (RTS; Scale 1-5) were obtained for heifers prior to assignment of one of five treatments: Non-synchronized + natural service (NS); melengestrol acetate + natural service (MGA + NS; 0.5 mg x animal-1 x d-1); 14-d controlled internal drug release + natural service (CIDR + NS; 1.38g progesterone); 14-d MGA-PG + fixed-time AI (FTAI); and 14-d CIDR-PG + FTAI. Heifers in the three NS treatments were exposed to fertile bulls for 65 d, beginning 10 d after progestin removal for CIDR + NS and MGA + NS groups. Heifers in FTAI treatments were administered PGF2[alpha] (PG; 25 mg, IM) 16 or 19 d following CIDR or MGA removal. Fixed-time AI was performed 66 and 72h after PG for CIDR-PG and MGA-PG treatments, respectively. Gonadotropin-releasing hormone (GnRH; 100[mu]g, i.m.) was administered at FTAI. Estrus detection aids were applied at PG for heifers in FTAI treatments and evaluated at AI. Heifers in FTAI treatments were exposed to fertile bulls 12 d following FTAI. Blood samples were collected and ovarian ultrasounds performed at PG administration and FTAI to compare serum concentrations of estradiol and progesterone and to evaluate follicular dynamics among a subset of heifers assigned to FTAI treatments. Pregnancy status was determined at the end of a 65-d breeding period. Data were analyzed using PROC FREQ and GLIMMIX procedures of SAS. Mean concentrations of estradiol at AI differed between MGA- versus CIDR-treated heifers (P = 0.04; 8.2 versus 6.6 pg/ml), however estrous response after PG (52% versus 53%) and pregnancy rates after FTAI (40%) did not differ between MGA- and CIDR-PG treatments, respectively. Across all treatments, pregnancy rates were compared on 21, 30 and 60 d of the breeding period based on pubertal status (prepubertal RTS = 1 and 2; peripubertal RTS = 3; pubertal RTS = 4 and 5). Pregnancy rates differed at each time point based on pretreatment pubertal status (P [less than or equal to] 0.02) and weight (P [less than or equal to] 0.05). No differences were apparent with regard to progestin type at any time point, although higher pregnancy rates (P [less than or equal to] 0.004) were observed among NS treatments than FTAI followed by NS exposure at Days 30 and 60. This experiment is the largest comprehensive field trial conducted in the United States and reported in the literature involving Bos indicus-influenced beef heifers utilizing estrus synchronization prior to natural service or FTAI. This study reinforced results from previous studies, however raises a number of questions yet to be answered for heifers of this biological type. These data should be used as a basis for improvements in reproductive management of Bos indicus-influenced beef heifers prior to their first breeding season, and creates a number of possibilities for future research trials that build upon results reported in this thesis. Selection, management, and post-weaning to pre-breeding development of Bos indicus-influenced replacement beef heifers should involve the cooperative efforts of beef producers, veterinarians, and research scientists to further investigate methods to improve reproductive efficiency and genetic merit of these herds.

201 USING THE TRANSCRIPTOME SIGNATURE TO PREDICT PREGNANCY SUCCESS IN BEEF COWS AT 6 DAYS AFTER ARTIFICIAL INSEMINATION

2015 ◽  
Vol 27 (1) ◽  
pp. 191
Author(s):  
S. Scolari ◽  
G. Pugliesi ◽  
S. C. S. Andrade ◽  
F. D'Alexandri ◽  
G. Gasparin ◽  
...  
Keyword(s):  
Pregnancy Outcome ◽  
Bos Indicus ◽  
Principal Component ◽  
Beef Cows ◽  
Endometrial Biopsy ◽  
Functional Enrichment ◽  
Marker Genes ◽  
High Fertility ◽  
Orthogonal Projections ◽  
Latent Structures

Pregnancy success is critical to the profitability of cattle operations. Attempts to reduce high rates of early embryonic loss mainly focus on the critical phase of embryo recognition by maternal tissue. However, the molecular events driving the uterine tissue toward a favourable stage, facilitating the maternal receptivity, are poorly understood. This study aimed to characterise the endometrial transcriptome profiles of pregnant versus nonpregnant beef cows during early pregnancy and attempted to define a potential set of marker genes that can be valuable for predicting pregnancy outcome. Therefore, pluriparous, cyclic Nellore (Bos indicus) cows were synchronized (n = 51) and artificially inseminated (n = 36) at detected oestrus using semen from a single high-fertility bull. Six days after AI (Day 6), jugular blood samples and an endometrial biopsy from the uterine horn contralateral to the ovary containing the CL were collected. Pregnancy diagnosis was performed by ultrasonography on Days 22 and 30. Based on pregnancy outcome, samples were retrospectively allocated to the following groups: pregnant (P; n = 6) and nonpregnant (NP; n = 5). Both groups had similar plasma progesterone concentrations on Day 6 (less than 1 ng mL–1 between lowest and greatest concentrations). Endometrial biopsies were submitted to RNA-Seq analysis in an Illumina single flow cell line (Illumina Inc., San Diego, CA). The 272 685 768 million filtered reads were mapped to the Bos taurus UMD3.1 reference genome and 14 654 genes were effectively analysed for differential expression between groups. Transcriptome data showed that 216 genes are differently expressed when comparing P v. NP endometrial tissue (adjusted P < 0.1). More specifically, 36 genes showed a significantly up-regulated expression for pregnant cows and 180 are up-regulated for non-pregant cows. Functional enrichment and pathway analyses revealed enriched expression of genes associated with extracellular matrix remodelling in NP cows and nucleotide binding, microsome, and vesicular fraction in P cows. From the 40 top-ranked genes, six that were down- and three that were up-regulated in pregnant cows were further analysed by qRT–PCR in an additional 26 cows. Subsequent quantitative expression data were evaluated using multivariate statistics. Both principal component analysis (PCA; R2 = 0.82 and Q2 = 0.40) and orthogonal projections to latent structures analysis (OPLS), using pregnancy as the dependent variable (R2Y = 0.95 and Q2 = 0.86), efficiently separated P from NP animals. In conclusion, this study characterizes a unique set of genes, expressed in the endometrium as early as 6 days after AI, that indicate a receptive state leading to pregnancy success. Furthermore, expression of such genes can be used as potential markers to efficiently predict pregnancy success.

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