scholarly journals 22 Puberty induction protocol, but not supplement amount, overcomes the negative impacts of reduced frequency of supplementation on reproduction of beef heifers

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 17-17
Author(s):  
Juliana Ranches ◽  
Marcelo Vedovatto ◽  
Elizabeth Palmer ◽  
Miguel Miranda ◽  
John Arthington ◽  
...  

Abstract This study evaluated the performance of Bos indicus-influenced beef heifers submitted to a puberty induction protocol and offered two supplementation amounts and delivery frequencies. Sixty-four Brangus crossbred heifers were stratified by BW and age (244 ± 4 kg; 311 ± 18 d) and assigned into 1 of 16 bahiagrass pastures on d 0. Treatments were randomly assigned to pastures in a 2 × 2 factorial design (4 pastures/treatment) and consisted of soyhulls-based supplementation at 1.25% or 1.75% of BW (DM basis) offered daily or 3 times weekly for 168 d. The puberty induction protocol consisted of CIDR insert on d 56 and removal on d 70, 5-mL PGF2a injection on d 86, 2-mL GnRH injection and timed-AI on d 89. Heifers were exposed to Angus bulls from d 89 to 167 (1 bull/pasture). Pregnancy diagnosis was assessed on d 212. Frequency × rate effects were not detected (P ≥ 0.19) for any variable. Reducing the supplementation frequency from daily to 3X weekly tended (P = 0.07) to decrease overall ADG and puberty attainment on d 56 (53 vs. 41 ± 6%), but not puberty attainment on d 89 (P = 0.45; 84 vs. 78 ± 6%) and pregnancy percentage on d 212 (P = 0.97; 69 vs. 69 ± 7%). Increasing the supplement amount from 1.25% to 1.75% of BW increased (P = 0.04) overall ADG, tended (P = 0.09) to hasten puberty attainment on d 56 (40 vs. 54 ± 6%) and d 89 (74 vs. 88 ± 6%), and enhanced (P = 0.03) pregnancy percentage on d 212 (53 vs. 80 ± 7%). Therefore, the inclusion of a puberty induction protocol, but not increasing the supplement amount, enabled a reduced frequency of supplementation (3 vs. 7 times weekly) without detrimental impacts on percentage of pregnant yearling beef heifers.

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 40-40
Author(s):  
Juliana Ranches ◽  
Marcelo Vedovatto ◽  
Elizabeth Palmer ◽  
Miguel Miranda ◽  
John Arthington ◽  
...  

Abstract This study evaluated the performance of Bos indicus-influenced beef heifers submitted to a puberty induction protocol and offered two supplementation amounts and delivery frequencies. Sixty-four Brangus crossbred heifers were stratified by BW and age (244 ± 4 kg; 311 ± 18 d) and assigned into 1 of 16 bahiagrass pastures on d 0. Treatments were randomly assigned to pastures in a 2 × 2 factorial design (4 pastures/treatment) and consisted of soyhulls-based supplementation at 1.25% or 1.75% of BW (DM basis) offered daily or 3 times weekly for 168 d. The puberty induction protocol consisted of CIDR insert on d 56 and removal on d 70, 5-mL PGF2a injection on d 86, 2-mL GnRH injection and timed-AI on d 89. Heifers were exposed to Angus bulls from d 89 to 167 (1 bull/pasture). Pregnancy diagnosis was assessed on d 212. Frequency × rate effects were not detected (P ≥ 0.19) for any variable. Reducing the supplementation frequency from daily to 3X weekly tended (P = 0.07) to decrease overall ADG and puberty attainment on d 56 (53 vs. 41 ± 6%), but not puberty attainment on d 89 (P = 0.45; 84 vs. 78 ± 6%) and pregnancy percentage on d 212 (P = 0.97; 69 vs. 69 ± 7%). Increasing the supplement amount from 1.25% to 1.75% of BW increased (P = 0.04) overall ADG, tended (P = 0.09) to hasten puberty attainment on d 56 (40 vs. 54 ± 6%) and d 89 (74 vs. 88 ± 6%), and enhanced (P = 0.03) pregnancy percentage on d 212 (53 vs. 80 ± 7%). Therefore, the inclusion of a puberty induction protocol, but not increasing the supplement amount, enabled a reduced frequency of supplementation (3 vs. 7 times weekly) without detrimental impacts on percentage of pregnant yearling beef heifers.


2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Philipe Moriel ◽  
Elizabeth Palmer ◽  
Marcelo Vedovatto ◽  
Matheus B Piccolo ◽  
Juliana Ranches ◽  
...  

Abstract This 2-yr study evaluated the growth and puberty attainment of Bos indicus-influenced beef heifers offered 2 different postweaning concentrate supplementation amounts and delivery frequencies. On day 0 of each year, 64 Brangus crossbred heifers were stratified by initial body weight (BW) and age (mean = 244 ± 22 kg; 314 ± 17 d) and assigned into 1 of 16 bahiagrass pastures (4 heifers/pasture/yr). Treatments were randomly assigned to pastures in a 2 × 2 factorial design (4 pastures/treatment/yr) and consisted of concentrate dry matter (DM) supplementation at 1.25% or 1.75% of BW which were offered either daily (7×) or 3 times weekly (3×) for 168 d. On day 56 of each year, heifers were assigned to an estrus synchronization protocol consisting of intravaginal controlled internal drug release (CIDR) insertion on day 56, CIDR removal on day 70, i.m. injection of 25 mg of prostaglandin F2α (PGF2α) on day 86, and i.m. injection of 100 µg of gonadotropin-releasing hormone and timed-AI at 66 h after PGF2α injection (day 89). Heifers were exposed to Angus bulls from day 89 to 168 (1 bull/pasture). Pregnancy diagnosis was assessed on day 213 of each year. Supplementation amount × frequency effects were not detected (P ≥ 0.12) for any variable, except for plasma concentrations of glucose (P = 0.10) and urea nitrogen (PUN; P = 0.01). Herbage mass, herbage allowance, and nutritive value did not differ (P ≥ 0.12) among treatments. Increasing supplementation DM amount from 1.25% to 1.75% of BW increased (P ≤ 0.05) plasma concentrations of insulin-like growth factor 1 (IGF-1), overall average daily gain (ADG), final BW, percentage of pubertal heifers on day 89, pregnancy and calving percentages, and percentage of heifers calving within the first 21 d of the calving season. However, reducing the supplementation frequency from daily to 3× weekly, regardless of supplementation amount, did not impact overall pregnancy and calving percentages (P ≥ 0.42), but caused (P ≤ 0.05) fluctuations in plasma concentrations of insulin and IGF-1 and decreased (P ≤ 0.03) overall ADG, final BW, puberty attainment on days 56, 89, and 168, and percentage of heifers calving during the first 21 d of the calving season. Hence, increasing the supplement DM amount did not prevent the negative effects of reducing the frequency of supplementation (3× vs. 7× weekly) on growth and reproduction of replacement Bos indicus-influenced beef heifers.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 309-310
Author(s):  
Felipe Alves Correa Carvalho da Silva ◽  
Gabriela da Silva ◽  
Bruna Vieira ◽  
Adomar Neto ◽  
Cecilia Constantino Rocha ◽  
...  

Abstract In cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Overarching hypothesis was that peri-estrus changes in ULF volume through accumulation and resorption mechanisms influence ULF composition during the estrous cycle and early pregnancy. Objectives were (1) to characterize individual temporal and spatial changes in ULF volume, endometrial and luteal vascularity, endometrial and luteal size, and progesterone (P4) concentrations during the peri-estrus period in beef heifers and, (2) associate such changes with the metabolite composition in the ULF, four days after estrus. Fourteen Bos indicus heifers that presented a PGF2α responsive CL received 500 µg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (estrus = d 0). Plasma P4 was measured daily. On d 4, the uterine body was sampled using a cytology brush for targeted metabolomic analysis by mass spectrometry. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Individual metabolite concentrations were compared between clusters A and B by univariate analysis using t-test after FDR correction. Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of non-essential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and associated uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers, which was associated with the ULF composition four days after estrus. Potential implications for embryo receptivity and reproductive outcomes are the focus of the current investigation.


2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 615-615
Author(s):  
G. M. Silva ◽  
P. Moriel ◽  
J. M. B. Vendramini ◽  
J. D. Arthington

2010 ◽  
Vol 22 (1) ◽  
pp. 177 ◽  
Author(s):  
M. Ramos ◽  
L. Cutaia ◽  
P. Chesta ◽  
G. A. Bó

Two experiments were designed to evaluate the effect of the timing of fixed-time AI (FTAI) in relation to the removal of an intravaginal progesterone-releasing device (1 g of progesterone, DIB, Syntex SA, Buenos Aires, Argentina) on pregnancy rates in Bos indicus × Bos taurus cross-bred heifers. In experiment 1, 285 Bonsmara × zebu cross-bred heifers, between 18 and 24 months of age and with a body condition score (BCS) between 3.0 and 3.5 (1-5 scale) were used. On the day of initiation of treatment (Day 0), the heifers’ ovaries were palpated (92% of them had a CL) and they received a new DIB plus 2 mg of estradiol benzoate (EB; Syntex SA) and 250 μg of cloprostenol (Ciclase DL, Syntex SA). On Day 8, DIB devices were removed and all heifers received 250 μg of Ciclase plus 0.5 mg of estradiol cypionate (ECP; Cipiosyn, Syntex SA). At that time the heifers were randomly divided to receive FTAI between 48 to 49 h, 53 to 54 h, or 58 to 59 h after DIB removal. The heifers underwent FTAI with semen from 4 bulls by 2 inseminators. In experiment 2, 260 heifers from the same group as those used in experiment 1 (87% with a CL) were treated exactly as those in experiment 1, except that previously used DIB was inserted on Day 0. Pregnancy diagnosis was performed 30 days post-fixed-time AI by ultrasonography. The data were analyzed by logistic regression, taking into account the effect of time of FTAI, semen, and inseminator on pregnancy rates. In experiment 1, pregnancy rates were lower (P = 0.04) in the heifers undergoing FTAI between 48 and 49 h after DIB removal (46/95, 48.4%) than those undergoing FTAI 53 to 54 h (61/99, 61.6%) or 58 to 60 h (57/91, 62.6%) after DIB removal. However, no differences in pregnancy rates were found (P = 0.72) in experiment 2 between the 3 treatment groups, with 39/91 (42.9%) for the 48 to 49 h group, 45/89 (50.6%) for the 53 to 54 h group, and 35/89 (43.8%) for the 58 to 59 h group. There was no effect of the semen or inseminator (P > 0.2) in either experiment. We conclude that when Bos indicus × Bos taurus beef heifers are synchronized with new DIB devices and ECP, higher pregnancy rates are obtained in heifers undergoing FTAI late (between 53 to 60 h after DIB removal) than in those undergoing FTAI early (48 to 49 h after DIB removal). However, time of insemination does not apparently affect pregnancy rates when Bos indicus × Bos taurus beef heifers are synchronized with previously used DIB devices and ECP.


2019 ◽  
Vol 31 (1) ◽  
pp. 134
Author(s):  
L. M. S. Simões ◽  
E. A. Lima ◽  
A. P. C. Santos ◽  
R. E. Orlandi ◽  
M. P. Bottino ◽  
...  

The objective was to determine the incidence of false-positive pregnancy diagnosis following the use of colour Doppler ultrasonography 20 and 22 days after fixed-time AI (FTAI) in Bos indicus heifers submitted to resynchronization 14 days after first FTAI. In the study, 512 Nellore heifers at 24.5±0.8 months of age and body condition score of 3.4±0.1 were used beginning 14 days after FTAI. On Day 14, heifers received 50mg of short-acting progesterone (Afisterone®, CEVA, São Paulo, Brazil) and a progesterone device (Prociclar®, CEVA). Eight days later (Day 22), the progesterone device was removed. A duplex B-mode (grayscale) and pulse-wave colour Doppler ultrasound instrument (M5, Mindray, Shenzhen, China) equipped with a multifrequency linear transducer was used for the examination of luteal blood flow on Days 20 and 22. The percentage of luteal area with colour Doppler signals of blood flow at each examination was determined as previously described (Ginther 2007) and was classified as low [corpus luteum (CL) with less than 25% vascularized area], intermediate (CL with 25-75% vascularized area), or high (CL with more than 75% vascularized area). Heifers with intermediate and high luteal blood flow were diagnosed as pregnant by Doppler ultrasonography, and heifers with low luteal blood flow were diagnosed as nonpregnant. Heifers diagnosed as pregnant by Doppler ultrasonography were examined 30 days after FTAI by B-mode ultrasound examination for pregnancy diagnosis and to determine the number of false positives (heifers pregnant by Doppler ultrasonography and nonpregnant by B-mode ultrasonography). Statistical analysis was performed by the GLIMMIX procedure of SAS (SAS Institute Inc., Cary, NC, USA). Pregnancy rate by Doppler ultrasonography [Day 20=60.5% (310/512), Day 22=55.3% (283/512); P=0.10] and false-positive percentage [Day 20=29.7% (92/310), Day 22=23.0% (65/2830); P=0.06] were similar on pregnancy diagnoses by Doppler ultrasonography performed 20 and 22 days after FTAI. Furthermore, in the period from 20 to 22 days after FTAI, luteolysis was verified in 5.8% (30/512) of heifers. In conclusion, anticipation of pregnancy diagnosis in 2 days by evaluation of luteal blood flow with colour Doppler ultrasonography (20 days after FTAI) does not interfere with pregnancy rate by Doppler ultrasound and percentage of false positives in Bos indicus heifers.


2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 371-372
Author(s):  
R Cooke ◽  
P Moriel ◽  
B Cappellozza ◽  
J Moraes Vasconcelos

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 73-73
Author(s):  
Alex Pursley ◽  
Bill Biligetu ◽  
Tom Warkentin ◽  
Bart Lardner ◽  
Greg B Penner

Abstract The objective was to evaluate the effect of inclusion rate of pea hay in barley and oat hay when fed to beef cattle. Six ruminally-cannulated heifers (407 ± 38 kg) were used in a 6 × 6 Latin square with a 2 × 3 factorial design and 25-d periods. Treatments included barley or oat hay blended with pea hay to achieve inclusion rates of 0, 15, or 30% (DM basis). Pea inclusion increased DMI (P = 0.03) by 0.75 kg/d, and generally reduced sorting (P ≤ 0.006) against NDF and ADF compared to the cereal-only treatments. Pea inclusion decreased CP digestibility by 2.87% relative to cereal-only treatments, but did not affect the predicted microbial protein supply or nitrogen retention (P ≥ 0.77). Pea inclusion did not affect total ruminal SCFA concentration, but increased the molar proportions of acetate and butyrate and decreased the molar proportion of propionate (P ≤ 0.01). While cereal type did not affect DMI (P = 0.36) or total SCFA concentration (P = 0.61), use of oat hay improved DM digestibility (67.73 vs. 63.22% for oat and barley, respectively; P < 0.001) and increased nitrogen retention (P = 0.03) when compared to barley. Overall, incorporating pea into cereal hay increased DMI, reduced CP digestibility, and altered ruminal fermentation.


PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0254119
Author(s):  
Jordan A. Drew ◽  
W. Owen Brimijoin

Those experiencing hearing loss face severe challenges in perceiving speech in noisy situations such as a busy restaurant or cafe. There are many factors contributing to this deficit including decreased audibility, reduced frequency resolution, and decline in temporal synchrony across the auditory system. Some hearing assistive devices implement beamforming in which multiple microphones are used in combination to attenuate surrounding noise while the target speaker is left unattenuated. In increasingly challenging auditory environments, more complex beamforming algorithms are required, which increases the processing time needed to provide a useful signal-to-noise ratio of the target speech. This study investigated whether the benefits from signal enhancement from beamforming are outweighed by the negative impacts on perception from an increase in latency between the direct acoustic signal and the digitally enhanced signal. The hypothesis for this study is that an increase in latency between the two identical speech signals would decrease intelligibility of the speech signal. Using 3 gain / latency pairs from a beamforming simulation previously completed in lab, perceptual thresholds of SNR from a simulated use case were obtained from normal hearing participants. No significant differences were detected between the 3 conditions. When presented with 2 copies of the same speech signal presented at varying gain / latency pairs in a noisy environment, any negative intelligibility effects from latency are masked by the noise. These results allow for more lenient restrictions for limiting processing delays in hearing assistive devices.


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