scholarly journals Full‐length mRNA sequencing and gene expression profiling reveal broad involvement of natural antisense transcript gene pairs in pepper development and response to stresses

2019 ◽  
Vol 99 (4) ◽  
pp. 763-783 ◽  
Author(s):  
Jubin Wang ◽  
Yingtian Deng ◽  
Yingjia Zhou ◽  
Dan Liu ◽  
Huiyang Yu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Antisense Transcript ◽  
Full Length ◽  
Natural Antisense Transcript ◽  
Mrna Sequencing ◽  
Gene Pairs ◽  
Transcript Gene

scholarly journals Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning

2000 ◽  
Vol 97 (17) ◽  
pp. 9543-9548 ◽  
Author(s):  
R.-M. Hu ◽  
Z.-G. Han ◽  
H.-D. Song ◽  
Y.-D. Peng ◽  
Q.-H. Huang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Cdna Cloning ◽  
Expression Profiling ◽  
Full Length ◽  
Human Hypothalamus ◽  
Full Length Cdna ◽  
Adrenal Axis ◽  
Hypothalamus Pituitary Adrenal Axis ◽  
Pituitary Adrenal Axis

The gene expression profiling of sporadic pheochromocytoma and novel full-length cDNAs cloning.

2003 ◽  
pp. 621-627
Author(s):  
Y-S Yang ◽  
H-D Song ◽  
Y-D Peng ◽  
Q-H Huang ◽  
R-Y Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adrenal Gland ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Molecular Mechanisms ◽  
Sequence Data ◽  
Full Length ◽  
Familial Pheochromocytoma ◽  
Sporadic Pheochromocytoma

Pheochromocytoma is a chromaffin cell neoplasm that typically causes symptoms and signs of episodic catecholamine release. Pheochromocytoma can be divided into two types: familial and sporadic. The molecular mechanisms involved in familial pheochromocytoma have been unraveled, but the detailed molecular mechanism of sporadic pheochromocytoma remains unknown. The present study thus aimed at characterization of gene expression profiling of sporadic pheochromocytoma using expressed sequence tags (ESTs), and established a preliminary catalog of genes expressed in the tumor. In total, 4115 ESTs were generated from the tumor library. The gene expression profilings of the pheochromocytoma and the normal adrenal gland were compared, and 341 genes were identified to be significantly expressed differently between the two libraries. Interestingly, 16 known genes participating in cell division or apoptosis were notably differently expressed between the tumor and the normal adrenal gland. Twenty-four novel full-length cDNAs were cloned from the tumor library and five of them were significantly up-regulated in the tumor. Some of them may be involved in the tumorigenesis of pheochromocytoma. The sequence data of ESTs and novel full-length cDNAs described in this paper have been submitted to the GeneBank library.

scholarly journals A Brassica Exon Array for Whole-Transcript Gene Expression Profiling

PLoS ONE ◽  
2010 ◽  
Vol 5 (9) ◽  
pp. e12812 ◽  
Author(s):  
Christopher G. Love ◽  
Neil S. Graham ◽  
Seosamh Ó Lochlainn ◽  
Helen C. Bowen ◽  
Sean T. May ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Exon Array ◽  
Transcript Gene

Gene expression profiling using a mouse full-length 20 K cDNA microarray

10.1038/14379 ◽  
1999 ◽  
Vol 23 (S3) ◽  
pp. 66-67 ◽  
Author(s):  
Yasushi Okazaki ◽  
Rika Miki ◽  
Yosuke Mizuno ◽  
Yasushiro Tomaru ◽  
Kouji Kadota ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Cdna Microarray ◽  
Expression Profiling ◽  
Full Length

Gene expression profiling in human insulinoma tissue: genes involved in the insulin secretion pathway and cloning of novel full-length cDNAs.

2004 ◽  
Vol 11 (2) ◽  
pp. 295-303 ◽  
Author(s):  
X-C Wang ◽  
S-Y Xu ◽  
X-Y Wu ◽  
H-D Song ◽  
Y-F Mao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Secretion ◽  
Molecular Biology ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Cdna Array ◽  
Full Length ◽  
Secretion Pathway ◽  
Est Sequencing ◽  
Human Insulinoma

Insulinoma is a clinically common cause of organic hypoglycemia. The prominent characteristic of insulinoma is endogenous hyperinsulinism. Until now, the molecular biology of human insulinoma has been little understood. In this study, gene expression profiling of human insulinoma was established by expressed sequence tag (EST) sequencing and cDNA array. A total of 2063 clones were obtained, of these, 1589 clones were derived from EST sequencing, 975 clones were derived from cDNA array and 501 clones were shared by the two methods. G protein alpha-stimulating activity polypeptide (Gsalpha) and carboxypeptidase E (CPE) were the most highly expressed genes in human insulinoma, as derived by EST sequencing and cDNA array respectively. The genes involved in the protein/insulin secretion pathway were strongly expressed in human insulinoma tissue. Meanwhile, eight full-length cDNAs of novel genes were cloned and sequenced. The results demonstrated the molecular biology of human insulinoma tissue at the level of transcript abundance and validated the efficacy of EST sequencing combined with cDNA array in the construction of gene expression profiling. In conclusion, the predominance of the genes participating in the secretory pathway suggested that regulation of secretion might be a major mechanism by which insulin release is abnormally increased in patients with insulinomas. It was also concluded that overexpression of the Gsalpha gene played an important role in the pathogenesis of insulinoma.

scholarly journals Gene expression profiling by mRNA sequencing reveals dysregulation of core genes in Rictor deficient T-ALL mouse model

2019 ◽  
Vol 87 ◽  
pp. 106229
Author(s):  
Chunlan Hua ◽  
Xiangyu Chen ◽  
Weiping Yuan ◽  
Yang Li ◽  
Jing Yu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mouse Model ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Mrna Sequencing ◽  
Core Genes

scholarly journals Gene expression profiling by mRNA sequencing reveals increased expression of immune/inflammation-related genes in the hippocampus of individuals with schizophrenia

2013 ◽  
Vol 3 (10) ◽  
pp. e321-e321 ◽  
Author(s):  
Y Hwang ◽  
J Kim ◽  
J-Y Shin ◽  
J-II Kim ◽  
J-S Seo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Mrna Sequencing ◽  
Immune Inflammation

Custom microarray for glycobiologists: considerations for glycosyltransferase gene expression profiling

2002 ◽  
Vol 69 ◽  
pp. 135-142 ◽  
Author(s):  
Elena M. Comelli ◽  
Margarida Amado ◽  
Steven R. Head ◽  
James C. Paulson
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Affymetrix Genechip ◽  
Microarray Technology ◽  
Gene Arrays ◽  
Glycosyltransferase Gene

The development of microarray technology offers the unprecedented possibility of studying the expression of thousands of genes in one experiment. Its exploitation in the glycobiology field will eventually allow the parallel investigation of the expression of many glycosyltransferases, which will ultimately lead to an understanding of the regulation of glycoconjugate synthesis. While numerous gene arrays are available on the market, e.g. the Affymetrix GeneChip® arrays, glycosyltransferases are not adequately represented, which makes comprehensive surveys of their gene expression difficult. This chapter describes the main issues related to the establishment of a custom glycogenes array.

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