scholarly journals Bioimaging of multiple elements by high‐resolution LA ‐ ICP ‐ MS reveals altered distribution of mineral elements in the nodes of rice mutants

2019 ◽  
Vol 99 (6) ◽  
pp. 1254-1263 ◽  
Author(s):  
Naoki Yamaji ◽  
Jian Feng Ma
Keyword(s):  
High Resolution ◽  
Mineral Elements ◽  
Icp Ms

scholarly journals Iron accumulation in the oculomotor nerve of the progressive supranuclear palsy brain

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hansol Lee ◽  
Myung Jun Lee ◽  
Eun-Joo Kim ◽  
Gi Yeong Huh ◽  
Jae-Hyeok Lee ◽  
...  
Keyword(s):  
High Resolution ◽  
Progressive Supranuclear Palsy ◽  
Oculomotor Nerve ◽  
Iron Accumulation ◽  
Blue Staining ◽  
Normal Brain ◽  
Myelinated Fiber ◽  
Healthy Controls ◽  
Icp Ms

AbstractAbnormal iron accumulation around the substantia nigra (SN) is a diagnostic indicator of Parkinsonism. This study aimed to identify iron-related microarchitectural changes around the SN of brains with progressive supranuclear palsy (PSP) via postmortem validations and in vivo magnetic resonance imaging (MRI). 7 T high-resolution MRI was applied to two postmortem brain tissues, from one normal brain and one PSP brain. Histopathological examinations were performed to demonstrate the molecular origin of the high-resolution postmortem MRI findings, by using ferric iron staining, myelin staining, and two-dimensional laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) imaging. In vivo iron-related MRI was performed on five healthy controls, five patients with Parkinson’s disease (PD), and five patients with PSP. In the postmortem examination, excessive iron deposition along the myelinated fiber at the anterior SN and third cranial nerve (oculomotor nerve) fascicles of the PSP brain was verified by LA-ICP-MS. This region corresponded to those with high R2* values and positive susceptibility from quantitative susceptibility mapping (QSM), but was less sensitive in Perls’ Prussian blue staining. In in vivo susceptibility-weighted imaging, hypointense pixels were observed in the region between the SN and red nucleus (RN) in patients with PSP, but not in healthy controls and patients with PD. R2* and QSM values of such region were significantly higher in patients with PSP compared to those in healthy controls and patients with PD as well (vs. healthy control: p = 0.008; vs. PD: p = 0.008). Thus, excessive iron accumulation along the myelinated fibers at the anterior SN and oculomotor nerve fascicles may be a pathological characteristic and crucial MR biomarker in a brain with PSP.

scholarly journals A comparison of annual layer thickness model estimates with observational measurements using the Berkner Island ice core, Antarctica

2017 ◽  
Vol 29 (4) ◽  
pp. 382-393
Author(s):  
A. Massam ◽  
S.B. Sneed ◽  
G.P. Lee ◽  
R.R. Tuckwell ◽  
R. Mulvaney ◽  
...  
Keyword(s):  
High Resolution ◽  
Layer Thickness ◽  
Inductively Coupled Plasma ◽  
Ice Core ◽  
Ultra Violet ◽  
Analytical Techniques ◽  
Inductively Coupled ◽  
Annual Layer ◽  
Icp Ms ◽  
Chemical Profiles

AbstractA model to estimate the annual layer thickness of deposited snowfall at a deep ice core site, compacted by vertical strain with respect to depth, is assessed using ultra-high-resolution laboratory analytical techniques. A recently established technique of high-resolution direct chemical analysis of ice using ultra-violet laser ablation inductively-coupled plasma mass spectrometry (LA ICP-MS) has been applied to ice from the Berkner Island ice core, and compared with results from lower resolution techniques conducted on parallel sections of ice. The results from both techniques have been analysed in order to assess the capability of each technique to recover seasonal cycles from deep Antarctic ice. Results do not agree with the annual layer thickness estimates from the age–depth model for individual samples <1 m long as the model cannot reconstruct the natural variability present in annual accumulation. However, when compared with sections >4 m long, the deviation between the modelled and observational layer thicknesses is minimized to within two standard deviations. This confirms that the model is capable of successfully estimating mean annual layer thicknesses around analysed sections. Furthermore, our results confirm that the LA ICP-MS technique can reliably recover seasonal chemical profiles beyond standard analytical resolution.

Trace elements distribution in tropical tree rings through high-resolution imaging using LA-ICP-MS analysis

2021 ◽  
Vol 68 ◽  
pp. 126872
Author(s):  
Renata S. Amais ◽  
Pedro S. Moreau ◽  
Danielle S. Francischini ◽  
Rafael Magnusson ◽  
Giuliano M. Locosselli ◽  
...  
Keyword(s):  
Trace Elements ◽  
High Resolution ◽  
Tree Rings ◽  
Tropical Tree ◽  
High Resolution Imaging ◽  
Ms Analysis ◽  
Icp Ms ◽  
Resolution Imaging

Multielemental Analysis of Purpleback Flying Squid Using High Resolution Inductively Coupled Plasma-Mass Spectrometry (HR ICP-MS)

2001 ◽  
Vol 35 (15) ◽  
pp. 3103-3108 ◽  
Author(s):  
Hideki Ichihashi ◽  
Hiroyoshi Kohno ◽  
Kurunthachalam Kannan ◽  
Akito Tsumura ◽  
Shin-ichi Yamasaki
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Inductively Coupled Plasma ◽  
Multielemental Analysis ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

scholarly journals QTL x environment interactions underlie ionome divergence in switchgrass

2021 ◽  
Author(s):  
Li Zhang ◽  
Alice MacQueen ◽  
Jason Bonnette ◽  
Felix B Fritschi ◽  
David B Lowry ◽  
...  
Keyword(s):  
Genetic Control ◽  
Linkage Mapping ◽  
Mapping Population ◽  
Mineral Elements ◽  
Native Range ◽  
Genotype By Environment ◽  
Common Gardens ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Biological Organisms

Abstract Ionomics measures elemental concentrations in biological organisms and provides a snapshot of physiology under different conditions. In this study, we evaluate genetic variation of the ionome in outbred, perennial switchgrass in three environments across the species’ native range, and explore patterns of genotype-by-environment interactions. We grew 725 clonally replicated genotypes of a large full sib family from a four-way linkage mapping population, created from deeply diverged upland and lowland switchgrass ecotypes, at three common gardens. Concentrations of 18 mineral elements were determined in whole post-anthesis tillers using ion coupled plasma mass spectrometry (ICP-MS). These measurements were used to identify quantitative trait loci (QTL) with and without QTL-by-environment interactions (QTLxE) using a multi-environment QTL mapping approach. We found that element concentrations varied significantly both within and between switchgrass ecotypes, and GxE was present at both the trait and QTL level. Concentrations of 14 of the 18 elements were under some genetic control, and 77 QTL were detected for these elements. 74% of QTL colocalized multiple elements, half of QTL exhibited significant QTLxE, and roughly equal numbers of QTL had significant differences in magnitude and sign of their effects across environments. The switchgrass ionome is under moderate genetic control and by loci with highly variable effects across environments.

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