OsUEV1B, an Ubc enzyme variant protein, is required for phosphate homeostasis in rice

2021 ◽  
Author(s):  
Jianping Liu ◽  
Wencheng Liao ◽  
Bo Nie ◽  
Jianhua Zhang ◽  
Weifeng Xu
Keyword(s):  
Phosphate Homeostasis ◽  
Variant Protein ◽  
Enzyme Variant

Differentiation dependent changes in the expression of a novel member of the ubiquitin enzyme variant family in the Caco-2 cell

2001 ◽  
Vol 120 (5) ◽  
pp. A500-A501
Author(s):  
G WILD ◽  
J FARIA ◽  
C COSSETTE ◽  
L DUFRESNE ◽  
A VILLANI ◽  
...  
Keyword(s):  
Enzyme Variant

1,25-DIHYDROXYVITAMIN D (1,25-(OH)2D) AND PARATHYROID HORMONE (PTH) IN CALCIUM AND PHOSPHATE HOMEOSTASIS: BIOCHEMICAL, PHYSIOLOGICAL AND CLINICAL ASPECTS.

1979 ◽  
Vol 92 (2_Supplb) ◽  
pp. S481-S487
Author(s):  
M.R. Haussler ◽  
M.K. Drezner ◽  
J.W. Pike ◽  
L.A. Hagan
Keyword(s):  
Parathyroid Hormone ◽  
Clinical Aspects ◽  
Phosphate Homeostasis ◽  
Dihydroxyvitamin D ◽  
1,25 Dihydroxyvitamin D

Cell-Specific Chemical Delivery Using a Selective Nitroreductase–Nitroaryl Pair

2018 ◽  
Author(s):  
Todd D. Gruber ◽  
Chithra Krishnamurthy ◽  
Jonathan B. Grimm ◽  
Michael R. Tadross ◽  
Laura M. Wysocki ◽  
...  
Keyword(s):  
Small Molecules ◽  
Mammalian Cells ◽  
Target Cells ◽  
Specific Chemical ◽  
E Coli ◽  
Enzyme Substrate ◽  
Cell Specificity ◽  
General Chemical ◽  
Increased Activity ◽  
Enzyme Variant

<p>The utility of<b> </b>small molecules to probe or perturb biological systems is limited by the lack of cell-specificity. ‘Masking’ the activity of small molecules using a general chemical modification and ‘unmasking’ it only within target cells could overcome this limitation. To this end, we have developed a selective enzyme–substrate pair consisting of engineered variants of <i>E. coli</i> nitroreductase (NTR) and a 2‑nitro-<i>N</i>-methylimidazolyl (NM) masking group. To discover and optimize this NTR–NM system, we synthesized a series of fluorogenic substrates containing different nitroaromatic masking groups, confirmed their stability in cells, and identified the best substrate for NTR. We then engineered the enzyme for improved activity in mammalian cells, ultimately yielding an enzyme variant (enhanced NTR, or eNTR) that possesses up to 100-fold increased activity over wild-type NTR. These improved NTR enzymes combined with the optimal NM masking group enable rapid, selective unmasking of dyes, indicators, and drugs to genetically defined populations of cells.</p>

Fibroblast growth factor 23 and its role in phosphate homeostasis in growing children compared to adults

2020 ◽  
Vol 33 (8) ◽  
pp. 1065-1071
Author(s):  
Marjan Jeddi ◽  
Maryam Heidari ◽  
Neda Hatami ◽  
Gholam Hossein Ranjbar Omrani
Keyword(s):  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Fibroblast Growth Factor 23 ◽  
Fractional Excretion ◽  
Cross Sectional Study ◽  
Fibroblast Growth ◽  
Sectional Study ◽  
Phosphate Homeostasis ◽  
Cross Sectional ◽  
Fractional Excretion Of Phosphate

AbstractObjectivesPhosphate is essential for skeletal mineralization, which is regulated by parathyroid hormone, calcitriol and fibroblast growth factor 23 (FGF23). Serum phosphate is physiologically higher in younger children, but factors that contribute to this physiological state are poorly understood. This study aimed to evaluate phosphate and its regulators in children compared with adults.Materials and methodsThe participants were children aged 3–11 years and adults older than 20 years of age. Biochemical parameters including calcium, phosphorus, alkaline phosphatase, FGF23, and vitamin D were measured. Fractional excretion of phosphate was calculated, using serum and urine phosphate and creatinine.ResultsThis cross-sectional study was conducted on 45 children (mean age: 9.0 ± 2.1) and 44 adults (mean age: 38.9 ± 11.1). The children had higher serum calcium, phosphate, alkaline phosphatase, and FGF23 (p < 0.001), but fractional excretion of phosphate was greater in adults (14.1 ± 5.7, 11.4 ± 4.4, p = 0.019, 95% confidence interval [CI]: −0.7 to −0.2). Of all individuals, 61.8% had vitamin D deficiency. By multiple regression analysis, entering age, calcium, phosphate, and vitamin D level, the only independent predictor of FGF23 was 1, 25 dihydroxy-vitamin D3 (β: 0.78, p < 0.001, 95% CI: 0.5–1.1, R2: 0.59 for children, and β: 0.59, p < 0.001, 95% CI: 0.5–1.4, R2: 0.45 for adults).ConclusionAs far as we know, there is little information regarding the role of FGF23 in physiologic state. In this cross-sectional study no association was found between FGF23 and urinary phosphate excretion in growing children. Further studies with more detail are essential to evaluate phosphate homeostasis during childhood.

scholarly journals CASEIN KINASE2-Dependent Phosphorylation of PHOSPHATE2 Fine-tunes Phosphate Homeostasis in Rice

2020 ◽  
Vol 183 (1) ◽  
pp. 250-262 ◽  
Author(s):  
Fei Wang ◽  
Meiju Deng ◽  
Jieyu Chen ◽  
Qiuju He ◽  
Xinye Jia ◽  
...  
Keyword(s):  
Phosphate Homeostasis

Nucleotide sequence of a variant protein A ofStaphylococcus aureus suggests molecular heterogeneity among strains

1991 ◽  
Vol 31 (5) ◽  
pp. 337-345 ◽  
Author(s):  
Marcelo De Macedo Brígido ◽  
Célia Regina Monte Barardi ◽  
Claudio A. Bonjardin ◽  
Cecília Luiza Simões Santos ◽  
Maria De Lourdes Junqueira ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Protein A ◽  
Molecular Heterogeneity ◽  
Ofstaphylococcus Aureus ◽  
Variant Protein
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