Biochemical and hematologic changes in whole blood from Brazilian horses stored in citrate–phosphate–dextrose–adenine pouches for up to 28 days

2021 ◽  
Author(s):  
Tagor E. A. Dorneles ◽  
Jair D. Costa Junior ◽  
Ricardo M. Almeida ◽  
Antonio R. Teixeira Neto
Keyword(s):  
Whole Blood ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

scholarly journals Assessment of Donkey (Equus asinus africanus) Whole Blood Stored in CPDA-1 and CPD/SAG-M Blood Bags

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 133
Author(s):  
Isabella Oliveira Barros ◽  
Rejane Santos Sousa ◽  
Marcondes Dias Tavares ◽  
Renato Otaviano Rêgo ◽  
Paulo Ricardo Firmino ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Whole Blood ◽  
Blood Collection ◽  
Blood Gas ◽  
Blood Cell Count ◽  
Animal Blood ◽  
Equus Asinus ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

Hemotherapy using whole blood and its components is being increasingly used in veterinary therapy. Since it is important to store animal blood while maintaining acceptable hematological, blood gas, and biochemical characteristics, increasing our knowledge of available technologies for strategic blood storage is imperative. Thus, we aimed to assess the hematological, blood gas, and biochemical changes in donkey whole blood using blood bags with two different types of storage agents. Eight adult healthy male donkeys were used; 900 mL of blood was collected from each, with 450 mL stored in citrate-phosphate-dextrose and adenine bags (CPDA-1) and 450 mL stored in bags containing citrate-phosphate-dextrose, adenine, mannitol, and sodium chloride (CPD/SAG-M). Both bags were kept refrigerated between 1 and 6 °C for 42 days. Blood samples were removed from the bags eight times (T): T0 (immediately after blood collection), T1, T3, T7, T14, T21, T35, and T42 (1, 3, 7, 14, 21, 35 and 42 days after storage). Hematological, blood gas, biochemical, and microbiological parameters were assessed. The CPDA-1 bags had a higher packed cell volume when compared to CPD/ SAG-M. The red blood cell count reduced by around 19% in both the bags due to hemolysis, which was confirmed by an increase in plasma hemoglobin. The white blood cell count; pH; concentrations of glucose, sodium, bicarbonate, and 2,3 diphosphoglycerate were reduced in both bags. Meanwhile, pO2, pCO2, lactate dehydrogenase, and levels of potassium increased in the CPDA-1 and CPD/SAG-M bags. Blood bags were efficient for the storage of donkey blood for up to 42 days.

scholarly journals Hematological, biochemical, and blood gas alterations of goat whole blood stored in CPDA-1 and CPD/SAG-M plastic bags

2019 ◽  
Vol 49 (10) ◽  
Author(s):  
Marcondes Dias Tavares ◽  
Isabella de Oliveira Barros ◽  
Rejane dos Santos Sousa ◽  
Paulo Ricardo Firmino ◽  
Jucelio da Silva Gameleira ◽  
...  
Keyword(s):  
Cell Count ◽  
Whole Blood ◽  
Hemoglobin Concentration ◽  
Blood Gas ◽  
Male Adult ◽  
Plasma Hemoglobin ◽  
Total Hemoglobin ◽  
Plastic Bags ◽  
Citrate Phosphate Dextrose ◽  
Citrate Phosphate

ABSTRACT: The aim of this study was to evaluate the hematological, biochemical, and blood gas alterations of goat whole blood stored in different blood bags. Seven male, adult, crossbreed goats were used, weighing 62±1.8 kg. Nine hundred milliliters of whole blood from each animal was collected and stored in blood bags (450 ml in each), CPDA-1 (citrate phosphate dextrose-adenine) and CPD/SAG-M (citrate phosphate dextrose with saline-glucose-mannitol) as additive solutions, and kept refrigerated (2-4 ºC) for 42 days. Blood samples were collected from the plastic bags at baseline (T0) and after seven, 14, 21,28, 35, and 42 days for hematological, biochemical, blood gas, and microbiological evaluations. Free hemoglobin, degree of hemolysis, lactate, and pO2were increased in both bags, whereas hydrogen potential (pH) and the total hemoglobin concentration decreased overtime(P<0.05). The red blood cell count, glucose, sodium, and potassium remained stable, compared to the baseline. The CPD/SAG-M bag presented a lower red cell count, globular volume, total hemoglobin, and sodium, and a higher degree of hemolysis and plasma hemoglobin, compared with the CPDA-1 bag. The whole goat blood remained viable for therapeutic use; although, there were some important changes in the variables of the 42-day stored blood in relation to fresh blood (T0). We concluded that the CPDA-1 bag is more suitable for use in the storage of goat blood because of its lower commercial value.

scholarly journals OBSERVATIONS ON THE BIOCHEMICAL EFFECTS OF TRANSFUSION OF CITRATE-PHOSPHATE-DEXTROSE STORED BLOOD IN MAN

1976 ◽  
Vol 86 (8) ◽  
pp. 1272-1279 ◽  
Author(s):  
Robert N. Miller ◽  
Robert Engelhardt ◽  
John A. Collins ◽  
Eduardo Slatopolsky ◽  
Jack H. Ladenson
Keyword(s):  
Biochemical Effects ◽  
Citrate Phosphate Dextrose ◽  
Stored Blood ◽  
Citrate Phosphate

scholarly journals Anticoagulants on yield of bone marrow-derived mononuclear cells harvested from dogs

2015 ◽  
Vol 45 (11) ◽  
pp. 2044-2049
Author(s):  
Harald Fernando Vicente de Brito ◽  
Tobias Fernandes-Filho ◽  
Fernanda de Lima Brandão ◽  
Patrícia Yukiko Montaño ◽  
Luciane Maria Laskoski ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Stem Cell ◽  
Iliac Crest ◽  
Mononuclear Cells ◽  
Cd44 Expression ◽  
Citrate Phosphate Dextrose ◽  
Healthy Dogs ◽  
Citrate Phosphate ◽  
Paired T Test

Cell therapy with bone marrow-derived mononuclear cells is an alternative to therapy with mesenchymal stem cell cultures. The aim of the present research was the comparison of the yield of bone marrow-derived mononuclear cells harvested from dogs with two different anticoagulants. Bone marrow was harvested from the iliac crest of five healthy dogs aged between 15 and 30 months, and the effect of two anticoagulant solutions, CPDA-1 (citrate phosphate dextrose adenine-1) and heparin, on the isolation of mononuclear cells was compared. Mononuclear cells were isolated in a density gradient and stained for CD9 and CD44 for characterization by flow cytometry. Means were compared using Student's paired t-test. Samples harvested with CPDA-1 yielded an average of 5.16x106 (±1.76x106) to 20.20x106 (±1.55x106) mononuclear cells/mL, whereas the yield of samples harvested with heparin varied between 4.56x106 (±0.69x106) and 24.30x106 (±2.12x106) mononuclear cells mL-1. By flow cytometry, mean percentage of double-stained cells varied from 1.96% (±0.64%) to 5.01% (±0.73%) for CPDA-1 and from 2.23% (±0.70%) to 7.27% (±0.97%) for heparin. No significant statistical differences were observed on yield or CD9 and CD44 expression. Further studies are recommended to assess efficacy of CPDA on mononuclear cell isolation.

Flow-dependent influence of high-O2-affinity erythrocytes on peak VO2 in exercising muscle in situ

1996 ◽  
Vol 80 (3) ◽  
pp. 832-838 ◽  
Author(s):  
H. Kohzuki ◽  
Y. Enoki ◽  
K. Matsumura ◽  
S. Sakata ◽  
S. Shimizu
Keyword(s):  
O2 Uptake ◽  
High Affinity ◽  
Staircase Effect ◽  
Citrate Phosphate Dextrose ◽  
High O2 ◽  
Rate Of Decline ◽  
O2 Delivery ◽  
Citrate Phosphate ◽  
Peak Value

To evaluate the influence of a high-O2 affinity of the erythrocyte and of flow rate on muscle's ability to extract O2 and develop force, we perfused dog gastrocnemius contracting isometrically at 4 Hz with normal-O2-affinity perfusate or high-O2-affinity perfusate at high and moderate flows (200 and 100 ml . min-1 . 100g-1, respectively). High-O2-affinity perfusate was prepared by incubating human citrate-phosphate-dextrose-stored erythrocytes with buffered saline containing cyanate (4 degrees C, 18 h) and normal-affinity perfusate by storing 2,3-diphosphoglycerate-rejuvenated erythrocytes in the same solution without cyanate. PO2 when blood is half oxygenated was 30.6 Torr for normal perfusate and 18.1 Torr for high-affinity perfusate. During 4-Hz stimulation, the tension developed by the muscle increased incrementally (positive staircase) to reach a peak value after 1.2-1.6 min for the normal perfusate and 0.6-0.7 min for the high-affinity perfusate (P < 0.05). The rate of decline during the early fatigue (measured from the onset of tension decline to 3 min) with high-affinity perfusate was significantly faster than it was with normal perfusate (P < 0.05). These findings suggest that both the staircase effect and the early fatigue are related to O2 availability, which is restricted when erythrocytes have a high O2 affinity. The peak O2 uptake values measured at 3 and 5 min were significantly lower (by 14-24%) with high-affinity perfusate than with normal perfusate at a given level of O2 delivery (arterial O2 content x flow) (P < 0.05). PO2 of venous effluent was proportionally related to peak O2 uptake. The present results indicate that neither blood flow nor O2 delivery is the sole determinant of the muscle's ability to extract O2.

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