scholarly journals Giardia duodenalis in colony stray cats from Italy

2021 ◽  
Author(s):  
Isabel Guadano Procesi ◽  
Azzurra Carnio ◽  
Federica Berrilli ◽  
Margherita Montalbano Di Filippo ◽  
Alessia Scarito ◽  
...  
2015 ◽  
Vol 53 (1) ◽  
pp. 119-124 ◽  
Author(s):  
Guochao Zheng ◽  
Wei Hu ◽  
Yuanjia Liu ◽  
Qin Luo ◽  
Liping Tan ◽  
...  

Широкое распространение безнадзорных животных на территории городов несет за собой потенциальную угрозу распространения зооантропонозных заболеваний, одним из которых является демодекоз. Невозможно разработать мероприятия, направленные на борьбу с заболеванием и его профилактику, без анализа данных особенностей возникновения и распространения инвазии среди всей популяции восприимчивых животных. Поэтому целью нашей работы явилось изучение распространения демодекоза среди безнадзорных собак и кошек в городе Тюмени. В задачи исследования входило изучение распространения демодекоза и его клинического проявления среди бездомных собак и кошек в условиях города Тюмени и определение сезонной динамики заболевания. Работу выполняли в 2016-2018 гг. на базе кафедры анатомии и физиологии ФГБОУ ВО ГАУ Северного Зауралья, в лаборатории акарологии ВНИИВЭА – филиала ТюмНЦ СО РАН, а также в производственных условиях на базе пункта временного содержания безнадзорных домашних животных МКУ «ЛесПаркХоз». Демодекозная инвазия распространена среди бездомных кошек и собак. Наиболее часто демодекоз встречается у собак, экстенсивность инвазии от 0,65 до 0,72%. Заболевание демодекозом у бездомных собак регистрировали на протяжении всего года, но 54,6% больных собак поступали в апреле и мае. Большинство больных демодекозом – это молодые собаки в возрасте от 1,5 месяцев до 2-х лет – 75,76%, животные старше двух лет гораздо реже страдали от демодекоза – 24,24%. Генерализацию демодекоза регистрировали у 21 собаки (63,64%), а локализованные очаги – у 12 собак (36,36%). Наиболее распространенной формой проявления демодекоза у бездомных собак является пустулезная, или пиодемодекоз. Данная форма заболевания была отмечена у 16 собак (48,49%), чешуйчатая форма отмечалась у 10 собак (30,30%), а смешанная – у 7 собак (21,21%). The widespread use of stray animals in urban areas carries with it the potential threat of the spread of zooanthroponotic diseases, one of which is demodicosis. It is impossible to develop measures aimed at combating the disease and its prevention without analyzing the data on the characteristics of the occurrence and spread of invasion among the entire population of susceptible animals. Therefore, the purpose of our work was to study the distribution of demodicosis among street dogs and cats in the city of Tyumen. The objectives of the study included the study of the spread of demodicosis and its clinical manifestation among stray dogs and cats in the conditions of the city of Tyumen and the determination of the seasonal dynamics of the disease. Demodectic invasion is common among stray cats and dogs. Most often, demodicosis occurs in dogs, with extensive invasion from 0.65 to 0.72%. Demodecosis in stray dogs was recorded throughout the year, but 54.6% of sick dogs were reported in April and May. The majority of patients with demodicosis are young dogs between the ages of 1.5 months and 2 years old - 75.76%, animals older than two years suffer less from demodicosis, only 24.24%. Generalization of demodicosis was recorded in 21 dogs (63.64%), and localized foci in 12 dogs (36.36%). The most common form of demodicosis in stray dogs is pustular or pyodemodecosis. This form of the disease was observed in 16 dogs (48.49%), scaly form, was observed in 10 dogs (30.30%), and mixed in 7 dogs (21.21%).


2020 ◽  
Vol 17 (4) ◽  
pp. 342-351
Author(s):  
Sergio A. Durán-Pérez ◽  
José G. Rendón-Maldonado ◽  
Lucio de Jesús Hernandez-Diaz ◽  
Annete I. Apodaca-Medina ◽  
Maribel Jiménez-Edeza ◽  
...  

Background: The protozoan Giardia duodenalis, which causes giardiasis, is an intestinal parasite that commonly affects humans, mainly pre-school children. Although there are asymptomatic cases, the main clinical features are chronic and acute diarrhea, nausea, abdominal pain, and malabsorption syndrome. Little is currently known about the virulence of the parasite, but some cases of chronic gastrointestinal alterations post-infection have been reported even when the infection was asymptomatic, suggesting that the cathepsin L proteases of the parasite may be involved in the damage at the level of the gastrointestinal mucosa. Objective: The aim of this study was the in silico identification and characterization of extracellular cathepsin L proteases in the proteome of G. duodenalis. Methods: The NP_001903 sequence of cathepsin L protease from Homo sapienswas searched against the Giardia duodenalisproteome. The subcellular localization of Giardia duodenaliscathepsin L proteases was performed in the DeepLoc-1.0 server. The construction of a phylogenetic tree of the extracellular proteins was carried out using the Molecular Evolutionary Genetics Analysis software (MEGA X). The Robetta server was used for the construction of the three-dimensional models. The search for possible inhibitors of the extracellular cathepsin L proteases of Giardia duodenaliswas performed by entering the three-dimensional structures in the FINDSITEcomb drug discovery tool. Results: Based on the amino acid sequence of cathepsin L from Homo sapiens, 8 protein sequences were identified that have in their modular structure the Pept_C1A domain characteristic of cathepsins and two of these proteins (XP_001704423 and XP_001704424) are located extracellularly. Threedimensional models were designed for both extracellular proteins and several inhibitory ligands with a score greater than 0.9 were identified. In vitrostudies are required to corroborate if these two extracellular proteins play a role in the virulence of Giardia duodenalisand to discover ligands that may be useful as therapeutic targets that interfere in the mechanism of pathogenesis generated by the parasite. Conclusion: In silicoanalysis identified two proteins in the Giardia duodenalisprotein repertoire whose characteristics allowed them to be classified as cathepsin L proteases, which may be secreted into the extracellular medium to act as virulence factors. Three-dimensional models of both proteins allowed the identification of inhibitory ligands with a high score. The results suggest that administration of those compounds might be used to block the endopeptidase activity of the extracellular cathepsin L proteases, interfering with the mechanisms of pathogenesis of the protozoan parasite Giardia duodenalis.


Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 503
Author(s):  
Ali Rostami ◽  
Seyed Mohammad Riahi ◽  
Vahid Fallah Omrani ◽  
Tao Wang ◽  
Andreas Hofmann ◽  
...  

Toxascaris leonina is an ascaridoid nematode of dogs and cats; this parasite affects the health of these animals. This study estimated the global prevalence of Ta. leonina infection in dogs and cats using random effects meta-analysis as well as subgroup, meta-regression and heterogeneity analyses. The data were stratified according to geographical region, the type of dogs and cats and environmental variables. A quantitative analysis of 135 published studies, involving 119,317 dogs and 25,364 cats, estimated prevalence rates of Ta. leonina in dogs and cats at 2.9% and 3.4%, respectively. Prevalence was highest in the Eastern Mediterranean region (7.2% for dogs and 10.0% for cats) and was significantly higher in stray dogs (7.0% vs. 1.5%) and stray cats (7.5% vs. 1.8%) than in pets. The findings indicate that, worldwide, ~26 million dogs and ~23 million cats are infected with Ta. leonina; these animals would shed substantial numbers of Ta. leonina eggs into the environment each year and might represent reservoirs of infection to other accidental or paratenic hosts. It is important that populations of dogs and cats as well as other canids and felids be monitored and dewormed for Ta. leonina and (other) zoonotic helminths.


Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 735
Author(s):  
João R. Mesquita ◽  
Ana C. Oliveira ◽  
Frederico Neves ◽  
Jose R. Mendoza ◽  
Maria F. Luz ◽  
...  

Free-roaming dogs and cats represent potential reservoirs for zoonotic vector-borne pathogens shedding to the human population. Given the health impact of these pathogens, we searched free-roaming dogs and cats included in an animal population control program from Luanda, Angola, for Bartonella and hemotropic mycoplasma infection. We report the detection of Bartonella henselae (2/66; 3%), Candidatus Mycoplasma haemominutum (5/66; 7.5%) and Mycoplasma haemofelis (1/66; 1.5%) in cats. One dog was found positive for Mycoplasma haemocanis (1/20; 5%). This is the first report of Bartonella henselae infections in stray cats and of hemotropic mycoplasmas in cats and dogs from Angola. Despite the relatively small sample size, our results sustain the hypothesis of uncontrolled circulation of these agents in highly mobile synanthropic animal populations of Luanda. Population and vector control could contribute to reducing the likelihood for animal-to-animal and animal-to-human transmission.


Author(s):  
Sahana Kuthyar ◽  
Martin M. Kowalewski ◽  
Matthew Seabolt ◽  
Dawn M. Roellig ◽  
Thomas R. Gillespie

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 940 ◽  
Author(s):  
Miller ◽  
Ward ◽  
Beatty

Measures aimed at reducing the length of stay (LOS) of cats in shelters can promote animal welfare and more efficient use of resources. The extent to which variables shown to impact LOS are broadly applicable is unclear. The aim of this study was to describe a population of cats adopted from an urban shelter, and to analyze the association between potential predictor variables and LOS. A study cohort was identified retrospectively from shelter records (n = 2584), 48.8% of which were < 12 weeks old at admission, and 80.7% were stray. Among 445 cats relinquished by owners, reasons for surrender were primarily owner-related (87.2%). Overall, reason for surrender and coat color were significantly associated with LOS. Hazard ratios showed that all reasons for surrender for owner-relinquished cats were associated with a shorter LOS than stray cats and this association was significant (p < 0.05) for all except cat behavioral or medical reasons. In contrast to previous reports, white cats had a significantly (p < 0.05) longer LOS than black cats. This study highlights an important role for shelter-specific baseline data to inform and measure the effect of interventional studies aimed at improving animal welfare by reducing LOS in shelter-housed cats


Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 206
Author(s):  
Pamela Carolina Köster ◽  
Antonio F. Malheiros ◽  
Jeffrey J. Shaw ◽  
Sooria Balasegaram ◽  
Alexander Prendergast ◽  
...  

Little information is available on the occurrence and genetic variability of the diarrhoea-causing enteric protozoan parasite Giardia duodenalis in indigenous communities in Brazil. This cross-sectional epidemiological survey describes the frequency, genotypes, and risk associations for this pathogen in Tapirapé people (Brazilian Amazon) at four sampling campaigns during 2008–2009. Microscopy was used as a screening test, and molecular (PCR and Sanger sequencing) assays targeting the small subunit ribosomal RNA, the glutamate dehydrogenase, the beta-giardin, and the triosephosphate isomerase genes as confirmatory/genotyping methods. Associations between G. duodenalis and sociodemographic and clinical variables were investigated using Chi-squared test and univariable/multivariable logistic regression models. Overall, 574 individuals belonging to six tribes participated in the study, with G. duodenalis prevalence rates varying from 13.5–21.7%. The infection was positively linked to younger age and tribe. Infected children <15 years old reported more frequent gastrointestinal symptoms compared to adults. Assemblage B accounted for three out of four G. duodenalis infections and showed a high genetic diversity. No association between assemblage and age or occurrence of diarrhoea was demonstrated. These data indicate that the most likely source of infection was anthropic and that different pathways (e.g., drinking water) may be involved in the transmission of the parasite.


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