scholarly journals Contrasting recruitment of skin‐associated adipose depots during cold challenge of mouse and human

2021 ◽  
Author(s):  
Ildiko Kasza ◽  
Jens‐Peter Kühn ◽  
Henry Völzke ◽  
Diego Hernando ◽  
Yaohui G. Xu ◽  
...  
Keyword(s):  
Cold Challenge

scholarly journals Kdm2a deficiency in macrophages enhances thermogenesis to protect mice against HFD-induced obesity by enhancing H3K36me2 at the Pparg locus

2021 ◽  
Author(s):  
Longmin Chen ◽  
Jing Zhang ◽  
Yuan Zou ◽  
Faxi Wang ◽  
Jingyi Li ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
High Fat Diet ◽  
Macrophage Polarization ◽  
Fatty Acid Uptake ◽  
Chromatin Accessibility ◽  
Acid Uptake ◽  
High Fat ◽  
M2 Polarization ◽  
Adipose Tissue Macrophages ◽  
Cold Challenge

AbstractKdm2a catalyzes H3K36me2 demethylation to play an intriguing epigenetic regulatory role in cell proliferation, differentiation, and apoptosis. Herein we found that myeloid-specific knockout of Kdm2a (LysM-Cre-Kdm2af/f, Kdm2a−/−) promoted macrophage M2 program by reprograming metabolic homeostasis through enhancing fatty acid uptake and lipolysis. Kdm2a−/− increased H3K36me2 levels at the Pparg locus along with augmented chromatin accessibility and Stat6 recruitment, which rendered macrophages with preferential M2 polarization. Therefore, the Kdm2a−/− mice were highly protected from high-fat diet (HFD)-induced obesity, insulin resistance, and hepatic steatosis, and featured by the reduced accumulation of adipose tissue macrophages and repressed chronic inflammation following HFD challenge. Particularly, Kdm2a−/− macrophages provided a microenvironment in favor of thermogenesis. Upon HFD or cold challenge, the Kdm2a−/− mice manifested higher capacity for inducing adipose browning and beiging to promote energy expenditure. Collectively, our findings demonstrate the importance of Kdm2a-mediated H3K36 demethylation in orchestrating macrophage polarization, providing novel insight that targeting Kdm2a in macrophages could be a viable therapeutic approach against obesity and insulin resistance.

Einsatz des niederenergetischen Laser bei Patienten mit primärem und sekundärem Raynaud-Phänomen

VASA ◽  
2001 ◽  
Vol 30 (4) ◽  
pp. 281-284 ◽  
Author(s):  
Mahdi Al-Awami ◽  
M. Schillinger ◽  
M. E. Gschwandtner ◽  
T. Maca ◽  
M. Haumer ◽  
...  
Keyword(s):  
Raynaud’S Phenomenon ◽  
Challenge Test ◽  
Raynaud's Phenomenon ◽  
Digital Ulcers ◽  
Invasive Treatment ◽  
Non Invasive ◽  
Level Laser ◽  
Digital Blood Flow ◽  
Cold Challenge ◽  
Digital Ischaemia

Background: Patients who had been treated with low level laser (LLL) for their digital ulcers reported an impressive improvement of their symptoms of episodic digital ischaemia. Therefore this pilot study was performed to evaluate the efficacy of LLL as a new non-drug non-invasive treatment for patients with primary and secondary Raynaud’s phenomenon (RP). Patients and methods: Forty patients (29 female, 11 male, mean age 51 years) with active primary (28%) and secondary (72%) Raynaud’s phenomenon received 10 sessions of LLL distant irradiation during winter months. Assessment of subjective and objective parameters was performed at baseline, one week after the last session and three months later. Variations of subjective parameters as number of daily acute episodes and severity of discomfort were assessed by a coloured visual analogue scale. A standardised cold challenge test using computed thermography of continuous temperature recordings by means of infrared telethermography was used to assess the digital blood flow. Results: A significant improvement was noticed clinically and thermographically after 6 weeks and 3 months, respectively (p < 0.0001). Conclusions: These data suggest that LLL treatment has a good short and medium term effectiveness in patients with Raynaud’s phenomenon.

Effects of Prenatal Maternal TRH Stimulation on the Postnatal Ability of Neonatal Piglets to Cope with a Cold Challenge

Neonatology ◽  
1998 ◽  
Vol 73 (6) ◽  
pp. 395-403
Author(s):  
A. Finsten ◽  
A. Donald ◽  
L.A. Bate
Keyword(s):  
Neonatal Piglets ◽  
Trh Stimulation ◽  
Cold Challenge

scholarly journals B-Cell-Activating Factor Depletion Ameliorates Aging-Dependent Insulin Resistance via Enhancement of Thermogenesis in Adipose Tissues

2020 ◽  
Vol 21 (14) ◽  
pp. 5121
Author(s):  
Bobae Kim ◽  
Chang-Kee Hyun
Keyword(s):  
Insulin Resistance ◽  
Glucose Tolerance ◽  
B Cell ◽  
Adipose Tissues ◽  
B Cell Activating Factor ◽  
Proliferation And Differentiation ◽  
Regulated Expression ◽  
Cold Challenge ◽  
Subcutaneous Adipose ◽  
Deficient Mice

Impaired glucose tolerance is a common feature associated with human aging, which is caused by defects in insulin secretion, insulin action or both. Recent studies have suggested that B-cell-activating factor (BAFF), a cytokine that modulates proliferation and differentiation of B cells, and its receptors are expressed in mature adipocytes and preadipocytes, proposing BAFF as a potential regulator of energy metabolism. In this study, we show that systemic BAFF depletion improves aging-dependent insulin resistance. In aged (10-month-old) BAFF−/− mice, glucose tolerance and insulin sensitivity were significantly improved despite higher adiposity as a result of expansion of adipose tissues compared to wild-type controls. BAFF−/− mice displayed an improved response to acute cold challenge, commensurate with the up-regulated expression of thermogenic genes in both brown and subcutaneous adipose tissues. These changes were found to be mediated by both increased M2-like (alternative) macrophage activation and enhanced leptin and FGF21 production, which may account for the improving effect of BAFF depletion on insulin resistance. In addition, leptin-deficient mice (ob/ob) showed augmented BAFF signaling concomitant with impaired thermogenic activity, identifying BAFF as a suppressive factor to thermogenesis. Our findings suggest that suppression of BAFF could be a therapeutic approach to attenuate aging-dependent insulin resistance.

Locating Perforator Vessels by Dynamic Infrared Imaging and Flow Doppler With No Thermal Cold Challenge

2011 ◽  
Vol 67 (2) ◽  
pp. 143-146 ◽  
Author(s):  
Xavier Tenorio ◽  
Ajay L. Mahajan ◽  
Badwi Elias ◽  
Julie Schaepkens van Riempst ◽  
Reto Wettstein ◽  
...  
Keyword(s):  
Infrared Imaging ◽  
Perforator Vessels ◽  
Cold Challenge

INDUCTION OF CUTANEOUS VASCULITIS BY REPEATED COLD CHALLENGE IN COLD URTICARIA

The Lancet ◽  
1978 ◽  
Vol 311 (8059) ◽  
pp. 336-337 ◽  
Author(s):  
R.A.J. Eady ◽  
M.W. Greaves
Keyword(s):  
Cutaneous Vasculitis ◽  
Cold Urticaria ◽  
Cold Challenge

Age-related differences in response to acute cold challenge (−10°C) in male F344 rats

1985 ◽  
Vol 20 (3-4) ◽  
pp. 201-209 ◽  
Author(s):  
Marilyn Kiang-Ulrich ◽  
Steven M. Horvath
Keyword(s):  
Age Related ◽  
Cold Challenge ◽  
F344 Rats ◽  
Male F344 Rats

Metabolic responses to tyramine and cold in young male Sprague-Dawley and Fischer 344 rats

1984 ◽  
Vol 246 (2) ◽  
pp. E141-E144 ◽  
Author(s):  
M. Kiang-Ulrich ◽  
S. M. Horvath
Keyword(s):  
Young Male ◽  
Strain Differences ◽  
Metabolic Responses ◽  
Fischer 344 Rats ◽  
Sprague Dawley ◽  
O2 Uptake ◽  
Fischer 344 ◽  
Cold Challenge ◽  
Colonic Temperature ◽  
F344 Rats

Significant differences in responses to intraperitoneally injected tyramine or cold exposure (-10 degrees C) were observed in young (3-4 mo) male Sprague-Dawley (S-D) and Fischer 344 (F344) rats cold-acclimated (CA) or non-cold-acclimated (non-CA). Non-CA S-D and F344 rats respond to tyramine by increased O2 uptake and elevation of colonic temperature, but, as we had reported previously, the optimal doses of tyramine required was significantly different for these two strains, i.e., 2 mg/kg for F344 vs. 20 mg/kg for S-D. The response to tyramine was significantly potentiated by cold acclimation in both strains of rats. Despite the different dosages of tyramine given to the animals, similar increases in O2 uptakes and colonic temperatures were observed. Differences between these two strains were also evident in their responses to a cold challenge (3 h, -10 degrees C). The ranking for cold tolerance (-10 degrees C) was as follows: CA F344 greater than non-CA F344 greater than CA S-D greater than non-CA S-D. These differences between the two strains suggest that interpretations regarding the response of rats to stressful conditions must be made with caution and appreciation of strain differences.

scholarly journals SUN-638 Perinatal DDE Exposure Disrupts Thermogenesis Early in Development

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Annalise vonderEmbse ◽  
Jason W Tong ◽  
Pamela J Lein ◽  
Michele La Merrill
Keyword(s):  
Body Weight ◽  
Area Under The Curve ◽  
Positive Association ◽  
Epidemiological Studies ◽  
Metabolic Phenotype ◽  
Infrared Analysis ◽  
Metabolic Dysfunction ◽  
Primary Metabolite ◽  
Brown Adipose ◽  
Cold Challenge

Abstract Numerous epidemiological studies have identified a positive association of exposure to the endocrine-disrupting pesticide dichlorodiphenyltrichloroethane (DDT) and its primary metabolite dichlorodiphenyldichloroethylene (DDE) with the risk of obesity. Of particular concern is the persistent metabolic dysfunction resulting from early life DDT and DDE exposures, evidenced as obesity, glucose intolerance, dyslipidemia, and impaired thermogenesis in adult rodents. However, little is known about the developmental timing and etiopathogenesis of this long-term DDT-related metabolic phenotype. This study aimed to address these knowledge gaps by evaluating endocrine phenotypes and thermogenic parameters at two postnatal (P) time points, P0 and P12, in C57BL/6J mice. Dams were orally gavaged with p,p’-DDT (1.7 mg/kg body weight [BW]), p,p’-DDE (1.31 mg/kg BW), or organic olive oil (vehicle) daily from gestational day (GD) 11.5-P0 or P5. Infrared analysis was then performed during a cold challenge. We further attempted to rescue the cold-challenged P12 mice by administering the β3-adrenergic receptor (AR)-agonist CL316,243 (CL), a direct stimulator of brown adipose (BAT) thermogenesis. At P0, area under the curve analysis revealed higher body temperatures in both males and females exposed to DDE compared to controls during the 9 min cold challenge. In addition, DDE-exposed females lost body heat at a significantly faster rate than sex-matched controls over the 9 min cold challenge. This occurred without any treatment-related differences in resting glucose, suprascapular BAT weight, or body weight for either sex at P0. To assess BAT-autonomous response to β3-AR stimulation during a cold challenge, P12 mice were exposed to the pharmacological β3-AR agonist, or PBS control in a 2x2 exposure design and suprascapular BAT temperature was evaluated via infrared analysis of the suprascapular surface at 10 min intervals. Blood glucose, BAT weight, and BW remained equivocal across all P12 treatment groups. However, BAT temperature was significantly decreased 10 min after cold- challenge in female P12 mice with perinatal DDE-exposure when compared to sex- and perinatal treatment-matched controls. This DDE effect among P12 female mice was rescued by CL. These data suggest that thermogenic dysfunction consequent to perinatal DDE exposure is detectable during postnatal development, well before the emergence of endocrine phenotypes. The CL rescue of the BAT thermogenic impairments observed after perinatal DDE exposure are consistent with DDE toxicities upstream in the β3-AR nerve circuitry. Additionally, the data highlight the emergence of an early postnatal sex divergence in DDE-related thermogenic dysfunction. We speculate that the effects of DDE on suprascapular BAT heat production result from prenatal alterations in sympathetic circuitry.

scholarly journals Central cardiovascular function during a cold challenge in raynaud's patients

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Guy Ball ◽  
Zahra Hussain ◽  
John E Davis
Keyword(s):  
Cardiovascular Function ◽  
Cold Challenge
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