scholarly journals Volume regulation in mammalian skeletal muscle: the role of sodium-potassium-chloride cotransporters during exposure to hypertonic solutions

2011 ◽  
Vol 589 (11) ◽  
pp. 2887-2899 ◽  
Author(s):  
Michael I. Lindinger ◽  
Matthew Leung ◽  
Karin E. Trajcevski ◽  
Thomas J. Hawke
Keyword(s):  
Skeletal Muscle ◽  
Potassium Chloride ◽  
Volume Regulation ◽  
Mammalian Skeletal Muscle ◽  
Sodium Potassium ◽  
Hypertonic Solutions

An integrative, in situ approach to examining K+ flux in resting skeletal muscle

2001 ◽  
Vol 79 (12) ◽  
pp. 996-1006 ◽  
Author(s):  
Michael I Lindinger ◽  
Thomas J Hawke ◽  
Lisa Vickery ◽  
Laurie Bradford ◽  
Shonda L Lipskie
Keyword(s):  
Skeletal Muscle ◽  
Drug Effects ◽  
Potassium Transport ◽  
Mammalian Skeletal Muscle ◽  
Minimal Effect ◽  
Bovine Erythrocyte ◽  
Perfusion Medium ◽  
Sodium Potassium ◽  
Unidirectional Flux

The contributions of Na+/K+-ATPase, K+ channels, and the NaK2Cl cotransporter (NKCC) to total and unidirectional K+ flux were determined in mammalian skeletal muscle at rest. Rat hindlimbs were perfused in situ via the femoral artery with a bovine erythrocyte perfusion medium that contained either 86Rb or 42K, or both simultaneously, to determine differences in ability to trace unidirectional K+ flux in the absence and presence of K+-flux inhibitors. In most experiments, the unidirectional flux of K+ into skeletal muscle (JinK) measured using 86Rb was 8–10% lower than JinK measured using 42K. Ouabain (5 mM) was used to inhibit Na+/K+-ATPase activity, 0.06 mM bumetanide to inhibit NKCC activity, 1 mM tetracaine or 0.5 mM barium to block K+ channels, and 0.05 mM glybenclamide (GLY) to block ATP-sensitive K+ (KATP) channels. In controls, JinK remained unchanged at 0.31 ± 0.03 µmol·g–1·min–1 during 55 min of perfusion. The ouabain-sensitive Na+/K+-ATPase contributed to 50 ± 2% of basal JinK, K+ channels to 47 ± 2%, and the NKCC to 12 ± 1%. GLY had minimal effect on JinK, and both GLY and barium inhibited unidirectional efflux of K+ (JoutK) from the cell through K+ channels. Combined ouabain and tetracaine reduced JinK by 55 ± 2%, while the combination of ouabain, tetracaine, and bumetanide reduced JinK by 67 ± 2%, suggesting that other K+-flux pathways may be recruited because the combined drug effects on inhibiting JinK were not additive. The main conclusions are that the NKCC accounted for about 12% of JinK, and that KATP channels accounted for nearly all of the JoutK, in resting skeletal muscle in situ.Key words: sodium potassium chloride cotransporter, NKCC, Na+/K+-ATPase, potassium channels, potassium transport, in situ rat hindlimb.

Hypothermia: alterations in cardiac and skeletal muscle electrolytes

1959 ◽  
Vol 196 (4) ◽  
pp. 706-708 ◽  
Author(s):  
W. Robert Beavers ◽  
J. T. Rogers
Keyword(s):  
Skeletal Muscle ◽  
Potassium Chloride ◽  
Cardiac Muscle ◽  
Normal Values ◽  
Intracellular Water ◽  
Sodium Potassium ◽  
Hypertonic Glucose ◽  
Plasma Electrolytes ◽  
Made In

Analyses of sodium, potassium, chloride and water of cardiac and skeletal muscle were made in normal dogs, in animals cooled to rectal temperatures of 20°C, and in cooled animals receiving 25% glucose intravenously. Using these data and determinations of plasma electrolytes, muscle intracellular water was calculated. An increase in cardiac muscle potassium and in calculated intracellular water of both cardiac and skeletal muscle was noted in the cooled animals. Administering hypertonic glucose during cooling increased cardiac muscle potassium to even higher levels and calculated intracellular water of cardiac and skeletal muscle was similar to normal values.

scholarly journals Role of the Cation-Chloride-Cotransporters in Cardiovascular Disease

Cells ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 2293 ◽  
Author(s):  
Nur Farah Meor Azlan ◽  
Jinwei Zhang
Keyword(s):  
Cardiovascular Disease ◽  
Potassium Chloride ◽  
Genetic Disorders ◽  
Ion Homeostasis ◽  
Cell Volume Regulation ◽  
Renal Cells ◽  
Sodium Potassium ◽  
Physiological Processes ◽  
Cation Chloride Cotransporters

The SLC12 family of cation-chloride-cotransporters (CCCs) is comprised of potassium chloride cotransporters (KCCs), which mediate Cl− extrusion and sodium-potassium chloride cotransporters (N[K]CCs), which mediate Cl− loading. The CCCs play vital roles in cell volume regulation and ion homeostasis. The functions of CCCs influence a variety of physiological processes, many of which overlap with the pathophysiology of cardiovascular disease. Although not all of the cotransporters have been linked to Mendelian genetic disorders, recent studies have provided new insights into their functional role in vascular and renal cells in addition to their contribution to cardiovascular diseases. Particularly, an imbalance in potassium levels promotes the pathogenesis of atherosclerosis and disturbances in sodium homeostasis are one of the causes of hypertension. Recent findings suggest hypothalamic signaling as a key signaling pathway in the pathophysiology of hypertension. In this review, we summarize and discuss the role of CCCs in cardiovascular disease with particular emphasis on knowledge gained in recent years on NKCCs and KCCs.

scholarly journals The all-or-none role of innervation in expression of apamin receptor and of apamin-sensitive Ca2+-activated K+ channel in mammalian skeletal muscle.

1985 ◽  
Vol 82 (7) ◽  
pp. 2188-2191 ◽  
Author(s):  
H. Schmid-Antomarchi ◽  
J. F. Renaud ◽  
G. Romey ◽  
M. Hugues ◽  
A. Schmid ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
K Channel ◽  
Mammalian Skeletal Muscle

scholarly journals The Movement of Solutes Across the Epithelium of the Ducts and Cisterns in the Mammary Gland of the Ewe

1965 ◽  
Vol 18 (5) ◽  
pp. 1035 ◽  
Author(s):  
DDS Mackenzie ◽  
AK Lascelles
Keyword(s):  
Mammary Gland ◽  
Water Absorption ◽  
Potassium Chloride ◽  
Chloride Ions ◽  
Rate Of Change ◽  
Milk Secretion ◽  
Sodium Potassium ◽  
Lactose Concentration

The role of the cisterns and large ducts in the overall process of milk secretion has been studied in experiments which have been carried out to determine the change in composition of solutions injected into the mammary gland cisterns of lactating and dry ewes. These solutions contained electrolytes, lactose, and a marker substance and were allowed to remain in the gland for periods of up to 4 hr. The concentration of sodium, potassium, chloride, and lactose in the solution injected into lactating glands approached with time that in milk. The data indicated that the rate of change in concentration of sodium, potassium, and chloride was more rapid than could be accounted for by the mixing of the injected solution with milk from the ducts. Sodium, potassium, and chloride ions were secreted into solutions in which the concentrations of these ions were lower than those in milk and sodium and chloride ions were absorbed from solutions in which their concentration was higher than that in milk. Water absorption occurred from the solutions containing sodium and chloride ata higher concentration than that in milk. In the dry gland the composition of the solutions injected approached with time that of involution secretion which has a higher sodium and chloride and lower potassium and lactose concentration than normal milk. Lactose was absorbed from dry glands but not from the lactating glands.

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