Application of Multifunctional Intelligent Suspension Treatment Beds in Nursing Care of Patients with Extensive Burns

The nursing care of patients with extensive burns by using multifunctional intelligent suspension treatment beds was studied. 40 patients, including 30 males and 10 females, with extensive burns were nursed using multifunctional intelligent suspension treatment beds. First of all, the patients were given psychological care, which was patiently explained, so that they can overcome their fears and be treated with peace of mind. Second, the room temperature and bed temperature were closely monitored. Finally, special attention was paid to the adjustment of rehydration volume, regular detection of plasma electrolytes, prevention of electrolyte disorder, and dehydration. Besides, disinfection and isolation should be performed when using. The results showed that 4 cases (20%) were positive in group A and 8 cases (40%) were positive in group B on the 10th day after injury X 2 = 4.005 , and the incidence of wound infection in group A was significantly lower than that in group B. The use of suspension beds in patients with extensive burns makes them safe and comfortable, and the whole body wound scabs healed faster, as well as the infection was minimized. A suspended bed is especially suitable for the clinical treatment of patients with extensive burns. The advantages of suspended bed can be fully realized by summarizing clinical experience.

ANALYSIS OF CHANGES IN ELECTROLYTE PARAMETERS IN WOMEN DURING IN VITRO FERTILIZATION PROGRAM

Aim of the research. To determine the concentration of basic plasma electrolytes in women in in vitro fertilization program who received more individualized medication correction on the basis of determination the clinical and pathogenetic role of disorders of the neuroendocrine regulation of water-salt metabolism, as well as to study data of the modern methods of prevention and correction of electrolyte disorders. Research materials. 110 patients in vitro fertilization program were examined. Conclusions. Thus, when using standard protocols for ovulation stimulation in in vitro fertilization program patients are more prone to have potassium and sodium imbalance. Ovarian hyperstimulation syndrome was detected only in patients of the main group with the standard ovulation induction protocols. This syndrome was not observed in patients receiving the combination therapy.

Electrochemical DNA Sensor Based on Acridine Yellow Adsorbed on Glassy Carbon Electrode

Electrochemical DNA sensors offer unique opportunities for the sensitive detection of specific DNA interactions. In this work, a voltametric DNA sensor is proposed on the base of glassy carbon electrode modified with carbon black, adsorbed acridine yellow and DNA for highly sensitive determination of doxorubicin antitumor drug. The signal recorded by cyclic voltammetry was attributed to irreversible oxidation of the dye. Its value was altered by aggregation of the hydrophobic dye molecules on the carbon black particles. DNA molecules promote disaggregation of the dye and increased the signal. This effect was partially suppressed by doxorubicin compensate for the charge of DNA in the intercalation. Sensitivity of the signal toward DNA and doxorubicin was additionally increased by treatment of the layer with dimethylformamide. In optimal conditions, the linear range of doxorubicin concentrations determined was 0.1 pM–1.0 nM, and the detection limit was 0.07 pM. No influence of sulfonamide medicines and plasma electrolytes on the doxorubicin determination was shown. The DNA sensor was tested on two medications (doxorubicin-TEVA and doxorubicin-LANS) and showed recoveries of 102–105%. The DNA sensor developed can find applications in the determination of drug residues in blood and for the pharmacokinetics studies.

CHANGES IN THE PLASMA ELECTROLYTES OF FRESH WATER TELEOST FISH LABEO ROHITA EXPOSED TO SUBLETHAL CONCENTRATION OF MONOSODIUM GLUTAMATE (MSG)

Background: Extensive usage of MSG in numerous food products as taste enhancer, and continuous release of efuents from Feed additive industries pose a threat to aquatic organisms.MSG induced cellular damages were reported in laboratory animals but there were no reports of MSG induced stress to shes. Methods: Fish Labeo rohita exposed to sublethal concentration of MSG (150mg/L) for 15 days and plasma electrolytes level were estimated. Results: MSG is toxic to sh even in sub lethal concentration. Decrease in plasma electrolytes during initial exposure to MSG may be due to + + disturbances in the membrane permeability of gills or inhibition of Na K ATP-ase activity in the gills. Elevated plasma electrolytes in MSG exposed sh may be due to efux of ions from their tissues there by indicating severe imbalance in the osmoregulation phenomena. Conclusion: MSG is toxic to sh and caused severe osmotic imbalance. Prolonged consumption of MSG through food as well as MSG intoxicated sh may affect human health via ecological cycling and biological magnication and cause serious health hazards like susceptibility to infectious diseases and even cancer

Insights into the origin and magnitude of capture and handling-related stress in a coastal elasmobranch Carcharhinus limbatus

A suite of blood chemistry parameters (including acid–base indicators and plasma electrolytes) was serially measured in blacktip sharks (Carcharhinus limbatus), captured via rod-and-reel, to gain a more thorough understanding of the physiological stress response to recreational capture. Sharks were caught both from the shore and from fishing vessels and experienced varying degrees of air exposure during handling. While all captured sharks exhibited a metabolic acidosis during the fight on the line (increasing lactate and decreasing pH and bicarbonate), the observed acidosis was compounded by a respiratory component (increasing pCO2) in sharks removed from the water during handling. Vessel-caught sharks handled in the water exhibited significantly greater increases in lactate and glucose (0.73 ± 0.21 mmoll−1 min−1 and 0.81 ± 1.07 mg dl−1 min−1, respectively) than sharks handled out of water (0.21 ± 0.17 mmoll−1 min−1 and −0.32 ± 1.05 mg dl−1 min−1; p < 0.001 and p < 0.05, respectively). These findings provide insights into how differences in recreational capture methods and air exposure can mediate the origin and magnitude of capture-related stress and highlight the importance of considering both sampling time (time from capture to phlebotomy) and sampling location (in water vs. out) in studies conducted on capture-related stress.

SGLT2 inhibition versus sulfonylurea treatment effects on electrolyte and acid–base balance: secondary analysis of a clinical trial reaching glycemic equipoise: Tubular effects of SGLT2 inhibition in Type 2 diabetes

Sodium–glucose transporter (SGLT)2 inhibitors increase plasma magnesium and plasma phosphate and may cause ketoacidosis, but the contribution of improved glycemic control to these observations as well as effects on other electrolytes and acid–base parameters remain unknown. Therefore, our objective was to compare the effects of SGLT2 inhibitors dapagliflozin and sulfonylurea gliclazide on plasma electrolytes, urinary electrolyte excretion, and acid–base balance in people with Type 2 diabetes (T2D). We assessed the effects of dapagliflozin and gliclazide treatment on plasma electrolytes and bicarbonate, 24-hour urinary pH and excretions of electrolytes, ammonium, citrate, and sulfate in 44 metformin-treated people with T2D and preserved kidney function. Compared with gliclazide, dapagliflozin increased plasma chloride by 1.4 mmol/l (95% CI 0.4–2.4), plasma magnesium by 0.03 mmol/l (95% CI 0.01–0.06), and plasma sulfate by 0.02 mmol/l (95% CI 0.01–0.04). Compared with baseline, dapagliflozin also significantly increased plasma phosphate, but the same trend was observed with gliclazide. From baseline to week 12, dapagliflozin increased the urinary excretion of citrate by 0.93 ± 1.72 mmol/day, acetoacetate by 48 μmol/day (IQR 17–138), and β-hydroxybutyrate by 59 μmol/day (IQR 0–336), without disturbing acid–base balance. In conclusion, dapagliflozin increases plasma magnesium, chloride, and sulfate compared with gliclazide, while reaching similar glucose-lowering in people with T2D. Dapagliflozin also increases urinary ketone excretion without changing acid–base balance. Therefore, the increase in urinary citrate excretion by dapagliflozin may reflect an effect on cellular metabolism including the tricarboxylic acid cycle. This potentially contributes to kidney protection.