scholarly journals Assessment of lignocellulosic residues from Northern Patagonian Andes (Argentina) for cultivation of Pleurotus ostreatus

2021 ◽  
Vol 26 (2) ◽  
Author(s):  
Maximiliano Rugolo ◽  
Juan Manuel Roggero-Luque ◽  
Mario Rajchenberg ◽  
Carolina Barroetaveña

This work evaluated mycelial growth rate (Kr) and fruiting of two Pleurotus ostreatus commercial strains (A01, 129) on formulations composed of lignocellulosic residues from farming and agroindustry of Northern Patagonian Andes, and of woody materials from invasive plants. Rosehip fluffs (RF), rosehip woodchips (RWC), southern beech wood shavings (SBWS), wheat straw (WS), and willow woodchips (WWC) were used as base substrates, and brewing bagasse (BB) as an alternative supplement to wheat bran (WB). Kr was higher in WS-WB and WS-BB for both strains. Experiments in fruiting chambers showed biological efficiencies (BEs) above 40% in WS-BB (both strains) and WS-WB (strain 129). Formulations using RWC or WWC gave BEs under 40%, while those composed of SBWS or RF showed lower Kr and contamination by moulds. Medium-scale fruiting experiments using strain A01 showed the highest BEs in WS-BB and RWC-WB. These results suggest that WS is the best substrate for Pleurotus ostreatus culture, although scarce in Northern Patagonian Andes. Nevertheless, WWC and RWC are suggested as alternative substrates, while BB is cheap and abundant, suitable as an alternative supplement to WB.

Author(s):  
Anaid Talavera-Ortiz ◽  
Priscila Chaverri ◽  
Gerardo Diaz-Godinez ◽  
Ma de Lourdes Acosta-Urdapilleta ◽  
Elba Villegas ◽  
...  

Abstract:Background and Aims: The production of edible fungi is affected by bacterial, fungal and viral diseases, which very often cause large losses. In the productionof mushrooms of the genus Pleurotus, the fungi of Trichoderma spp. represent a serious problem of contamination and although there are some chemical compoundsthat control the infection, they are not entirely safe for human consumption. As a consequence, alternatives are being searched for through biotechnology,such as the one presented in this paper.Methods: Strains of fungi of the genus Trichoderma were isolated from the substrate where Pleurotus ostreatus was being cultivated. These were identifiedmorphologically and molecularly, followed by tests to inhibit the growth of Trichoderma strains in both agar and wheat straw, using a cetonic extract of thedehydrated fruiting body of Pycnoporus sp.Key results: Two strains of Trichoderma (T. pleuroti and T. atrobrunneum, belonging to the clade of T. harzianum) were isolated from infected substrate obtainedin production modules of Pleurotus ostreatus located in Tlaquitenango and Cuernavaca, in the state of Morelos, Mexico. The effect of a cetonic extract of thefruiting body of Pycnoporus sp. on the mycelial growth of the isolated strains of Trichoderma was also evaluated, observing decrease in mycelial growth rate inPetri dish up to 72% and on lignocellulosic substrate both mycelial growth and sporulation were delayed up to 10 days.Conclusions: The extract of Pycnoporus sp. could be an alternative to control the infection by Trichoderma spp. in mushroom cultures of the genus Pleurotus.Key words: disease control, edible mushrooms, lignocellulosic substrate. 


2014 ◽  
Vol 45 (3) ◽  
pp. 861-872 ◽  
Author(s):  
P.C. Guadarrama-Mendoza ◽  
G. Valencia del Toro ◽  
R. Ramírez-Carrillo ◽  
F. Robles-Martínez ◽  
J. Yáñez-Fernández ◽  
...  

2021 ◽  
Vol 13 (4) ◽  
pp. 120
Author(s):  
Karla Danielle Nogueira Cardoso ◽  
Maria Josiane Martins ◽  
Thaisa Aparecida Neres de Souza ◽  
Isabelle Carolyne Cardoso ◽  
Patrícia Nirlane da Costa Souza ◽  
...  

Phomopsis sojae and Sclerotinia sclerotiorum are responsible for stem and pod dryness and white mold in soybean. These pathologies directly affect the quality of seeds/grains and compromise the entire plant. The use of extracts from different plants has been the subject of research for the control of several phytopathogens. Calotropis procera is among botanical species that synthesize efficient compounds for biocontrol. In this context, the aim of this study was to evaluate the in vitro effect of C. procera aqueous extract on P. sojae and S. sclerotiorum. The experiment was carried out in completely randomized blocks in a 2 × 5 factorial scheme (two fungi and five extract concentrations 0%, 5%, 10%, 15% and 20%) with 4 replicates. C. procera aqueous extract concentrations were added to Petri dishes containing PDA. After 48 hours, the mycelial growth rate was evaluated. After seven days of incubation, the fungal colony area, sporulation, and germination of P. sojae and S. sclerotiorum were evaluated. There was significant interaction between fungi × extract concentrations (p < 0.05) for all variables analyzed. The mycelial growth rate of P. sojae was lower than that of S. sclerotiorum. The diameter of the P. sojae fungal colony was smaller than that of S. sclerotiorum when concentrations of 5%, 10% and 15% were used. As the extract concentration increased, fungi sporulation and germination reduced.


2019 ◽  
Vol 74 (11-12) ◽  
pp. 811-818 ◽  
Author(s):  
Lingling Fan ◽  
Bilan Luo ◽  
Zhongfu Luo ◽  
Li Zhang ◽  
Judi Fan ◽  
...  

AbstractIn order to obtain novel bioactive compounds with significant antifungal activities, two series of 3-substituted phthalide derivatives were designed and synthesized via reduction, bromine substitution, and etherification. In addition, the antifungal activities of all target compounds against nine phytopathogenic fungi in vitro were tested by using the mycelial growth rate method at the concentration of 50 μg mL−1. Preliminary bioassay tests showed that some compounds exhibited more potent antifungal activities as compared with hymexazol. The preliminary structure-activity relationships (SARs) of all target compounds were also investigated.


2014 ◽  
Vol 49 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Nooraishah Harith ◽  
Noorlidah Abdullah ◽  
Vikineswary Sabaratnam

The objective of this work was to evaluate the feasibility of different agro-residues as a carbon source in the fruiting substrates of Flammulina velutipesmushroom and the effect of supplementation with the nitrogen sources spent brewer's yeast and rice bran. The following fruiting substrates were evaluated: rubber wood sawdust (SD), paddy straw (PS), palm empty fruit bunches (EFB), and palm-pressed fiber (PPF). Cultivation was done on each agro-residue, based on formulations consisting of two substrates at the ratios of 3:1, 1:1, and 1:3. Mycelial growth rate and basidiocarp yield were evaluated. The best fruiting substrates were PS+EFB (25:75), PS+PPF (50:50), and PPF (100), with biological efficiency of 185.09±36.98, 150.89±50.35, and 129.06±14.51%, respectively. No significant effects of supplementation with rice bran and spent yeast were observed on mycelial growth rate and biological efficiency. The cultivation of F. velutipes on oil palm wastes does not require additional nitrogen sources.


2015 ◽  
Vol 64 (6) ◽  
pp. 1450-1460 ◽  
Author(s):  
S. E. Meyer ◽  
M. Masi ◽  
S. Clement ◽  
T. L. Davis ◽  
J. Beckstead

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