Micro Rod Mixer Using AC Electric Field Induced Flow

Author(s):  
Hiromichi Obara ◽  
Shinichi Tashiro

A novel rod mixer, using a micro-scale flow structure induced with electrokinetics under an AC electric field, is proposed for three-dimensional micro-scale processing of biological applications. Flow structures around the micro-rod mixer are demonstrated using the micro-particle image velocimetry method and the particle-tracking velocimetry method. Characteristics of the flow structure and mixing property are investigated for several electric field intensities and frequencies. It is observed that the micro-mixing flow structures induced with an AC electric field at the appropriate intensity and frequency conditions which are intermediate conditions between a dielectrophoresis and an electrolysis. A source-type three-dimensional flow structure is generated around the tip of the micro-rod electrode installed in the sample liquid. Furthermore it is possible to control the scale of the flow structure by adjusting the supplied AC electric field intensity and frequency. The mixing characteristics are also discussed.

Author(s):  
Hiromichi Obara ◽  
Ryousuke Ibata ◽  
Yusuke Kawai ◽  
Yasuaki Matsudair

The microscopic characteristics of a functional fluid for a micro polishing process were investigated by microscopic observation and micro particle image velocimetry. This functional fluid, which consists of suspended micro scale diamond particles in insulated silicon oil, has two specific flow structures under a high-voltage alternating electric field. One flow structure consists of a reciprocating flow generated in one direction between the electrodes under low-electric-field and high-frequency conditions. The other flow structure consists of a rotational flow formed under high-electric-field and low-frequency conditions. These specific flow structures contribute actively to a polishing process and to the development of micro fluidic devices in the future. In the present study, the effect of the electric field on specific flow structures and the mechanism of the induced flow are clarified.


Author(s):  
Kanji Kaneko ◽  
Takayuki Osawa ◽  
Yukinori Kametani ◽  
Takeshi Hayakawa ◽  
Yosuke Hasegawa ◽  
...  

The steady streaming (SS) phenomenon is gaining increased attention in the microfluidics community, because it can generate net mass flow from the zero-mean vibration. We developed numerical simulation and experimental measurement tools to analyze this vibration induced flow, which has been challenging due to its unsteady nature. Validity of these analysis methods is confirmed by comparing the three-dimensional (3D) flow field induced around a cylindrical micropillar under circular vibration. In the numerical modeling, we directly solved the flow in the Lagrangian frame so that the substrate with a micropillar becomes stationary, and the result was converted to the Eulerian frame to compare them with the experimental results. The present approach enables to avoid the introduction of moving boundary or small perturbation approximation. The flow field obtained by the micro particle image velocimetry (PIV) measurement supported the three-dimensionality observed in the numerical results, which could be important for controlling the mass transport and manipulating particulate objects in the microfluidic systems.


2006 ◽  
Author(s):  
Renqiang Xiong ◽  
J. N. Chung

Flow structures and pressure drops were investigated in rectangular serpentine micro-channels with miter bends which had hydraulic diameters of 0.209mm, 0.395mm and 0.549mm respectively. To evaluate the bend effect, the additional pressure drop due to the miter bend must be obtained. Three groups of micro-channels were fabricated to remove the inlet and outlet losses. A validated micro-particle image velocimetry (μPIV) system was used to achieve the flow structure in a serpentine micro-channel with hydraulic diameter of 0.173mm. The experimental results show the vortices around the outer and inner walls of the bend do not form when Re<100. Those vortices appear and continue to develop with the Re number when Re> 100-300, and the shape and size of the vortices almost remain constant when Re>1000. The bend loss coefficient Kb was observed to be related with the Re number when Re<100, with the Re number and channel size when Re>100. It almost keeps constant and changes in the range of ± 10% When Re is larger than some value in 1300-1500. And a size effect on Kb was also observed.


Micromachines ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 668 ◽  
Author(s):  
Kanji Kaneko ◽  
Takayuki Osawa ◽  
Yukinori Kametani ◽  
Takeshi Hayakawa ◽  
Yosuke Hasegawa ◽  
...  

The steady streaming (SS) phenomenon is gaining increased attention in the microfluidics community, because it can generate net mass flow from zero-mean vibration. We developed numerical simulation and experimental measurement tools to analyze this vibration-induced flow, which has been challenging due to its unsteady nature. The validity of these analysis methods is confirmed by comparing the three-dimensional (3D) flow field and the resulting particle trajectories induced around a cylindrical micro-pillar under circular vibration. In the numerical modeling, we directly solved the flow in the Lagrangian frame so that the substrate with a micro-pillar becomes stationary, and the results were converted to a stationary Eulerian frame to compare with the experimental results. The present approach enables us to avoid the introduction of a moving boundary or infinitesimal perturbation approximation. The flow field obtained by the micron-resolution particle image velocimetry (micro-PIV) measurement supported the three-dimensionality observed in the numerical results, which could be important for controlling the mass transport and manipulating particulate objects in microfluidic systems.


2006 ◽  
Vol 505-507 ◽  
pp. 343-348
Author(s):  
C.T. Pan ◽  
P.J. Cheng ◽  
Yeong-Maw Hwang ◽  
M.F. Chen ◽  
H.S. Chuang ◽  
...  

A self-built micro-particle image velocimetry (micro-PIV) with a diode laser is established to measure the micro-fluidic phenomenon in a 100 μm rectangular capillary. By scanning method, a 3-D flow image with a flowrate of 0.3 μL/min is presented. With this calibration method, the measurement ability for 3-D micro-fluidic dynamics could be achieved. This technique also reveals its benefit and potential in metrology. Hence, it provides a helpful tool for Bio-MEMS research. The experiment is proceeded under laminar flow, Re= 0.011. The measurement range is ranging from 0.05μm/s to 4.3mm/s. The vector grid resolution is optimized to 2.5 μm.


Author(s):  
Wael Fairouz Saleh ◽  
Ibrahim Galal Hassan

The discharge of two-phase flow from a stratified region through single or multiple branches is an important process in many industrial applications including the pumping of fluid from storage tanks, shell-and-tube heat exchangers, and the fluid flow through small breaks in cooling channels of nuclear reactors during loss-of-coolant accidents (LOCA). Knowledge of the flow phenomena involved along with the quality and mass flow rate of the discharging stream(s) is necessary to adequately predict the different phenomena associated with the process. Particle Image Velocimetry (PIV) in three dimension was used to provide detailed measurements of the flow patterns involving distributions of mean velocity, vorticity field, and flow structure. The experimental investigation was carried out to simulate two phase discharge from a stratified region through branches located on a semi-circular wall configuration during LOCA scenarios. The semi-circular test section is in close dimensional resemblance with that of a CANDU header-feeder system, with branches mounted at orientation angles of zero, 45 and 90 degrees from the horizontal. The experimental data for the phase development (mean velocity, flow structure, etc.) was done during single discharge through the bottom branch from an air/water stratified region over a three selected Froude numbers. These measurements were used to describe the effect of outlet flow conditions on phase redistribution in headers and understand the entrainment phenomena.


2015 ◽  
Vol 656-657 ◽  
pp. 549-553
Author(s):  
Kyohei Nishimoto ◽  
Kozo Taguchi

Dielectrophoresis (DEP) force will arise when an inhomogeneous AC electric field with sinusoidal wave is applied to microelectrodes. By using DEP, we could distinguish between viable and non-viable cells by their movement through a non-uniform electric field. In this paper, we propose a yeast cell separation system, which utilizes an Au DEP chip and an optical tweezers. The Au DEP chip is planar quadrupole microelectrodes, which were fabricated by Au thin-film and a box cutter. This fabrication method is low cost and simpler than previous existing methods. The tip of the optical tweezers was fabricated by dynamic chemical etching in a mixture of hydrogen fluoride and toluene. The optical tweezers has the feature of high manipulation performance. That does not require objective lens for focusing light because the tip of optical tweezers has conical shape. By using both the Au DEP chip and optical tweezers, we could obtain three-dimensional manipulation of specific cells after viability separation.


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