Microfabricated Cavities for Adhesive Capture of Stem Cells Under Flow

2007 ◽  
Author(s):  
Dooyoung Lee ◽  
Kuldeepsinh Rana ◽  
Karin Lee ◽  
Lisa A. DeLouise ◽  
Michael R. King
Keyword(s):  
Fluid Dynamics ◽  
Stem Cells ◽  
Computational Fluid Dynamics ◽  
Blood Flow ◽  
Shear Stresses ◽  
Computational Fluid Dynamics Cfd ◽  
Future Work ◽  
Flow Experiments

In previous work, we have described the adhesive capture of circulating stem cells to surfaces coated with adhesive selectin protein, both in vitro and in vivo. Here we describe PDMS surfaces microfabricated to contain an array of square 80 × 80 × 80 micron cavities. These cavities are intended to provide a local bioreactor environment to culture stem cells over extended periods of time, while sheltered from the higher shear stresses of the surrounding blood flow external of the cavities. In this paper we present in vitro flow experiments with polymeric, blood cell-sized microspheres, showing the creation of stable vortices within the microscale cavities. Computational fluid dynamics (CFD) was performed to predict the velocity field within the cavity, and for comparison with experimentally determined microsphere velocities. Future work will establish the ability to place local chemoattract molecules within the cavity interior, and the ability to accumulate viable stem cells within these cavities.

Rhinomanometry Versus Computational Fluid Dynamics: Correlated, but Different Techniques

2020 ◽  
pp. 194589242095015
Author(s):  
Giancarlo B. Cherobin ◽  
Richard L. Voegels ◽  
Fábio R. Pinna ◽  
Eloisa M. M. S. Gebrim ◽  
Ryan S. Bailey ◽  
...  
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Nasal Resistance ◽  
Nasal Patency ◽  
Low Correlation ◽  
Before And After ◽  
Computational Fluid Dynamics Cfd ◽  
Flow Experiments

Background Past studies reported a low correlation between rhinomanometry and computational fluid dynamics (CFD), but the source of the discrepancy was unclear. Low correlation or lack of correlation has also been reported between subjective and objective measures of nasal patency. Objective: This study investigates (1) the correlation and agreement between nasal resistance derived from CFD (RCFD) and rhinomanometry (RRMN), and (2) the correlation between objective and subjective measures of nasal patency. Methods Twenty-five patients with nasal obstruction underwent anterior rhinomanometry before and after mucosal decongestion with oxymetazoline. Subjective nasal patency was assessed with a 0-10 visual analog scale (VAS). CFD simulations were performed based on computed tomography scans obtained after mucosal decongestion. To validate the CFD methods, nasal resistance was measured in vitro (REXPERIMENT) by performing pressure-flow experiments in anatomically accurate plastic nasal replicas from 6 individuals. Results Mucosal decongestion was associated with a reduction in bilateral nasal resistance (0.34 ± 0.23 Pa.s/ml to 0.19 ± 0.24 Pa.s/ml, p = 0.003) and improved sensation of nasal airflow (bilateral VAS decreased from 5.2 ± 1.9 to 2.6 ± 1.9, p < 0.001). A statistically significant correlation was found between VAS in the most obstructed cavity and unilateral airflow before and after mucosal decongestion (r = −0.42, p = 0.003). Excellent correlation was found between RCFD and REXPERIMENT (r = 0.96, p < 0.001) with good agreement between the numerical and in vitro values (RCFD/REXPERIMENT = 0.93 ± 0.08). A weak correlation was found between RCFD and RRMN (r = 0.41, p = 0.003) with CFD underpredicting nasal resistance derived from rhinomanometry (RCFD/RRMN = 0.65 ± 0.63). A stronger correlation was found when unilateral airflow at a pressure drop of 75 Pa was used to compare CFD with rhinomanometry (r = 0.76, p < 0.001). Conclusion CFD and rhinomanometry are moderately correlated, but CFD underpredicts nasal resistance measured in vivo due in part to the assumption of rigid nasal walls. Our results confirm previous reports that subjective nasal patency correlates better with unilateral than with bilateral measurements and in the context of an intervention.

Computational Fluid Dynamics (CFD) Study of the 4th Generation Prototype of a Continuous Flow Ventricular Assist Device (VAD)

2004 ◽  
Vol 126 (2) ◽  
pp. 180-187 ◽  
Author(s):  
Xinwei Song ◽  
Houston G. Wood ◽  
Don Olsen
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Blood Flow ◽  
Ventricular Assist Device ◽  
Continuous Flow ◽  
Surrounding Tissue ◽  
Assist Device ◽  
Ventricular Assist ◽  
Wide Range ◽  
Computational Fluid Dynamics Cfd

The continuous flow ventricular assist device (VAD) is a miniature centrifugal pump, fully suspended by magnetic bearings, which is being developed for implantation in humans. The CF4 model is the first actual prototype of the final design product. The overall performances of blood flow in CF4 have been simulated using computational fluid dynamics (CFD) software: CFX, which is commercially available from ANSYS Inc. The flow regions modeled in CF4 include the inlet elbow, the five-blade impeller, the clearance gap below the impeller, and the exit volute. According to different needs from patients, a wide range of flow rates and revolutions per minute (RPM) have been studied. The flow rate-pressure curves are given. The streamlines in the flow field are drawn to detect stagnation points and vortices that could lead to thrombosis. The stress is calculated in the fluid field to estimate potential hemolysis. The stress is elevated to the decreased size of the blood flow paths through the smaller pump, but is still within the safe range. The thermal study on the pump, the blood and the surrounding tissue shows the temperature rise due to magnetoelectric heat sources and thermal dissipation is insignificant. CFD simulation proved valuable to demonstrate and to improve the performance of fluid flow in the design of a small size pump.

Abstract 372: The Endothelial Glycocalyx Promotes Homogenous Blood Flow Distribution within the Microvasculature

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
P Mason McClatchey
Keyword(s):  
Fluid Dynamics ◽  
Blood Flow ◽  
Blood Viscosity ◽  
Tissue Perfusion ◽  
Microvascular Perfusion ◽  
Endothelial Glycocalyx ◽  
Heterogeneous Distribution ◽  
Disease States

Introduction: Impaired tissue oxygenation is observed in many disease states including congestive heart failure, diabetes, cancer and aging. Decreased tissue perfusion and heterogeneous distribution of blood flow in the microvasculature contributes to this pathology. The physiological mechanisms regulating homogeneity/heterogeneity of microvascular perfusion are presently unknown. We hypothesized that microfluidic properties of the glycocalyx would promote perfusion homogeneity. Methods: To test our hypothesis, we used established empirical formulations for modelling blood viscosity in vivo (blood vessels) and in vitro (glass tubes). We first assess distribution of blood flow in idealized arteriolar networks. We next simulated distribution of blood flow at an idealized capillary bifurcation. Finally, we simulated velocity profiles and pressure gradients within the vessel lumen with varying glycocalyx properties using a computational fluid dynamics approach. Results: We found that transit time heterogeneity (as assessed by STD to mean ratio) was increased approximately 9x (6.9x-10.6x) using in vitro formulations of blood viscosity relative to in vivo formulations. This effect was mathematically accounted for by increased effective blood viscosity in smaller arterioles. We also found that distribution of blood flow at an idealized microvascular bifurcation was more symmetric using the in vivo formulation than the in vitro formulation (approximately 2x greater disparity between flow in downstream vessels). This effect was mathematically accounted for by an increased hematocrit dependence of blood viscosity. Both the diameter- and hematocrit-based changes in blood viscosity were entirely predictable from fluid dynamics simulations incorporating a space-filling, semi-permeable glycocalyx layer. Summary: Our simulations indicate that the mechanical properties of the endothelial glycocalyx promote homogeneous microvascular perfusion. Conclusions: The literature provides evidence of both glycocalyx degradation and impaired tissue perfusion in the same disease states. Preservation or restoration of normal glycocalyx properties may be a viable strategy for improving tissue perfusion in a wide variety of diseases.

The Design of Refinement Criteria for Dynamic Grid Adaptation Applied to Airfoil Flow Modeling

2003 ◽  
Author(s):  
Melih Demir ◽  
Govert de With ◽  
Arne E. Holdo̸
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Flow Field ◽  
Unsteady Flow ◽  
Mesh Refinement ◽  
Research Centre ◽  
Shear Stresses ◽  
Wall Region ◽  
Grid Adaptation ◽  
Computational Fluid Dynamics Cfd

At present a large number of fluid dynamics applications are found in aerospace, civil and automotive engineering, as well in medical related fields. In many applications the flow field is turbulent and the computational modelling of such flows remains a difficult task. To resolve all turbulent flow phenomena for flow problems where turbulence is of key interest is a priori not feasible in a Computational Fluid Dynamics (CFD) investigation with a conventional mesh. The use of a Dynamic Grid Adaptation (DGA) algorithm in a turbulent unsteady flow field is an appealing technique which can reduce the computational costs of a CFD investigation. A refinement of the numerical domain with a DGA algorithm requires reliable criteria for mesh refinement which reflect the complex flow processes. At present not much work has been done to obtain reliable refinement criteria for turbulent unsteady flow. The purpose of the work is to implement a new refinement technique for the boundary layer in the vicinity of the wall. It is aimed to model the flow around an airfoil with a LES turbulence model and a new DGA algorithm. In addition to that several simulations have been carried out for parametric studies. In these studies the incompressible solver in REACFLOW has been used. This Computational Fluid Dynamics (CFD) code REACFLOW was developed in collaboration with the joint Research Centre (JRC) in Italy. The following aims are aspired: • A new mesh refinement criteria method suitable for boundary layers; • To carry out LES simulations to establish the performance of the refinement criteria. The new criteria which are created in this work are for the near wall region. This criteria uses the wall shear stresses for the refinement technique. For the main flow stream the refinement criteria proposed by de With et al [6] will be used.

Numerical Simulations of the Non-Newtonian Blood Blow in Human Abdominal Artery Based on Reverse Engineering

2011 ◽  
Vol 140 ◽  
pp. 195-199 ◽  
Author(s):  
Jin You YANG ◽  
Yang Hong
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Blood Flow ◽  
Fluid Flow ◽  
Reverse Engineering ◽  
Power Law ◽  
Cardiac Cycle ◽  
Hemodynamic Parameters ◽  
Human Artery ◽  
Computational Fluid Dynamics Cfd

The method that combined the reverse engineering based on CT medical images and computational fluid dynamics (CFD) was used to perform simulation the Non-Newtonian blood fluid flow in human abdominal artery, then analyzed the hemodynamic condition about the bifurcation of human abdominal artery. A Non-Newtonian blood model (the Generalised Power Law) was used to study the hemodynamic parameters during entire cardiac cycle. Calculated results for the Non-Newtonian blood flow show us the methods performed in this study is suitable for numerical simulating the blood flow in human artery and investigating the relation between hemodynamic factors and vascular disease.

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