Single Cell Electrophysiological Analysis via Sub-Micrometer Openings

2005 ◽  
Author(s):  
Sha Li ◽  
Liwei Lin
Keyword(s):  
Cervical Cancer ◽  
Hela Cell ◽  
Yeast Cell ◽  
Single Cell ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell ◽  
Cervical Cancer Cell Line ◽  
Electrophysiological System

Single cell electrophysiological analyses were demonstrated via sub-micrometer openings using micro-and nano-machining technologies. In the prototype demonstration, a 6GΩ seal resistance was achieved on a HeLa cell, a cervical cancer cell line with size between 10~20μm, using the microfabricated electrophysiological system with opening size of 500nm in diameter and the measured electrolyte pipette resistance is 200kΩ. In a second experiment on a vacuolar yeast cell of 3~5μm in size, using a device with 800nm opening, a 500MΩ seal resistance was achieved and the measured electrolyte pipette resistance is 1MΩ.

scholarly journals Retraction: Tetraarsenic Oxide-mediated Apoptosis in a Cervical Cancer Cell Line, SiHa

2007 ◽  
Vol 39 (1) ◽  
pp. 48
Author(s):  
Jeong Kim ◽  
Su-Mi Bae ◽  
Dae-Seog Lim ◽  
Sun-Young Kwak ◽  
Chang-Ki Lee ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell ◽  
Cervical Cancer Cell Line

Benzylisoquinoline alkaloids from Nelumbo nucifera Gaertn. petals with antiausterity activities against the HeLa human cervical cancer cell line

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Juthamart Maneenet ◽  
Ashraf M. Omar ◽  
Sijia Sun ◽  
Min Jo Kim ◽  
Supawadee Daodee ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Nelumbo Nucifera ◽  
Cervical Cancer Cell ◽  
Cervical Cancer Cell Line ◽  
Benzylisoquinoline Alkaloids ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

Abstract Ethanolic extract of Nelumbo nucifera petals showed preferential cytotoxic activity against HeLa human cervical cancer cell line with a PC50 value of 10.4 μg/mL. This active extract was subjected to a phytochemical investigation study which led to the isolation of nine benzylisoquinoline alkaloids (1–9). The isolated compounds exhibited potent antiausterity activities. Moreover, under nutrient-deprived conditions, (−)-lirinidine (8) induced remarkable alterations in HeLa cell morphology including cell shrinkage and plasma blebbing leading to total cell death within 10 h. Mechanistically, 8 was found to inhibit Akt/mTOR signaling pathway. It also induced apoptosis by promoting caspase-3 activation and inhibiting Bcl-2 expression. Therefore, benzylisoquinoline alkaloids skeleton can be considered as a promising scaffold for the anticancer drug development against cervical cancer.

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