Abstract A120: Morphological assessment of apoptosis in HeLa cervical cancer cell line by Dox-loaded magnetic nanocluster.

Spons merupakan fauna laut yang diketahui memiliki berbagai senyawa bioaktif. Senyawa tersebut berpotensi sebagai antibakteri, antivirus, dan antikanker. Penelitian ini bertujuan mempelajari aktivitas sitotoksik dan apoptosis ekstrak spons spesies A anggota ordo Astrophorida terhadap sel HeLa. Pada penelitian ini pengujian aktivitas sitotoksik ekstrak etanolik, metanolik, dan kloroform spons spesies A terhadap sel HeLa dilakukan menggunakan MTT assay dan uji apoptosis menggunakan double staining, yaitu etidium bromida-acridine orange. Deteksi golongan senyawa yang terkandung di dalam spons spesies A dilakukan menggunakan Kromatografi Lapis Tipis (KLT). Hasil uji sitotoksisitas menunjukkan bahwa ekstrak etanolik, metanolik, dan kloroform spons spesies A masing-masing memiliki nilai IC50 sebesar 18,25; 27,87; dan 13,87 Âµg/mL. Ekstrak etanolik, metanolik, dan kloroform spons spesies A pada konsentrasi 31,25 Âµg/mL dapat menginduksi kematian sel melalui apoptosis masing-masing sebesar 35,3 Â± 11,16%; 82,64 Â± 16,21%; dan 86,76 Â± 9,27%. Berdasarkan uji menggunakan KLT diketahui bahwa spons spesies A menggandung golongan senyawa alkaloid, flavonoid, fenol, dan terpenoid. Oleh karena itu, dapat disimpulkan bahwa ekstrak spons spesies A berpotensi untuk dikembangkan sebagai obat antikanker.Kata kunci: ekstrak spons spesies A, sitotoksik, apoptosis, sel HeLa

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Selected Sudanese Medicinal Plants Induce Anticancer and Cytotoxic Effects in Cervical Cancer Cell Line

Cancer Research and Cellular Therapeutics ◽

10.31579/2640-1053/031 ◽

2018 ◽

Vol 2 (3) ◽

pp. 01-04

Author(s):

Montasir Elnour

Keyword(s):

Cervical Cancer ◽

Cell Line ◽

Cancer Cell ◽

Antioxidant Activities ◽

Cancer Cell Line ◽

Cervical Cancer Cell ◽

Cervical Cancer Cell Line ◽

Plant Parts ◽

Lotus Leaves ◽

Hela Cervical Cancer Cell

Modern pharmacology, however, relies on refined chemicals - either obtained from plants, or synthesized. This work investigated the anticancer, antioxidant and Cytotoxicity activities of N. lotus leaves citrate, N. lotus leaves not citrate, P. juliflora leaves commonly used as anti-inflammatory and Ant diabetic. All the plant parts were extracted using 80% methanol, the anticancer activity was examined by using MTT assay against Hela (Cervical Cancer) Cell Line. And determine their antioxidant activities by testing DPPH cytotoxicity using - (4, 5-Dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), filter and kept in dark, prepared freshly. The examined plants methanolic extracts of P. juliflora leaves is high activity against Hela (Cervical Cancer) Cell Line IC50 is 56.02 µg/ml. The extract C N. lotus leaves citrate, N. lotus leaves not citrate has shown none active anti- Hela (Cervical Cancer) IC50 values 100, and > 100 μg/ml respectively. All the extracts revealed cytotoxicity activity not toxic in N. lotus leaves citrate, N. lotus leaves not citrate, P. juliflora leaves the inhibition percentage with (90.81, 89.33074 , 86.47866 ) (73.17427 , 71.93975 , 60.17069 ) (74.93001, 73.78714 , 71.13981 ) respectively. The following plant parts showed highly potent scavenging activity against DPPH (above 80%); N. lotus leaves citrate, 88.78, While the following revealed a good activity against DPPH (between 60-79); N. lotus leaves not citrate, P. juliflora leaves with inhibition % are 77and 62.96 respectively.

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