scholarly journals Molecular data confirm the presence of Nycticebus bengalensis on Langkawi Island, Malaysia

2019 ◽  
Vol 20 (4) ◽  
pp. 1115-1120
Author(s):  
BADRUL MUNIR MD ZAIN ◽  
KHAIRUL SYA’ADAH MOHHOYUA ◽  
NOR RAHMAN AIFAT ◽  
EHWAN NGADI ◽  
NORSHAQINAH AYOB ◽  
...  
Keyword(s):  
Significant Contribution ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Peninsular Malaysia ◽  
Coi Gene ◽  
Slow Loris ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Tree Topologies ◽  
High Bootstrap

Abstract. Md-Zain BM, Mohhoyua KS, Aifat NR, Ngadi E, Ayob N, Rovie-Ryan JJ, Ampeng A, Mohd-Ridwan AR, Blair ME, Abdul-Latiff MAB. 2019. Molecular data confirm the presence of Nycticebus bengalensis on Langkawi Island, Malaysia. Biodiversitas 20: 1115-1120. Recent taxonomic reviews have stated the possibility of Bengal Slow Loris (Nycticebus bengalensis) presence in the Northern part of the Malay Peninsula. This study aims to confirm the presence of the Bengal Slow Loris in Malaysia by sequencing the mitochondrial COI gene from samples collected from Langkawi Island, Peninsular Malaysia, and Borneo. Phylogenetic analyses produced tree topologies that support the grouping of slow loris samples by their localities. The tree topologies further show that slow loris samples from Sarawak and Peninsular Malaysia form two distinct clades. The clade from Peninsular Malaysia was divided into two subclades, Langkawi and Selangor. The Langkawi slow loris subclade includes sequences from GenBank representing N. bengalensis, supported by a high bootstrap value. This mitochondrial DNA finding has a significant contribution to indicate the presence of the Bengal Slow Loris in Malaysia.

Taxonomic status of Euphylliidae corals in Peninsular Malaysia based on morphological structures and phylogenetic analyses using mitochondrial COI gene

2017 ◽  
Vol 43 (2) ◽  
pp. 118-126 ◽  
Author(s):  
Khodzori Fikri Akmal ◽  
Saad Shahbudin ◽  
Nordin Noor Faizul Hadry ◽  
Ku Sulong Ku Abdullah ‘Ulwan ◽  
Mat Zain Khairul Amira
Keyword(s):  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Peninsular Malaysia ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

Are Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara (Acari: Tetranychidae) one species? Morphological and molecular evidences for synonymy between these two spider mite species and a note on invasiveness of T. macfarlanei on okra and eggplant in India

2017 ◽  
Vol 22 (4) ◽  
pp. 467
Author(s):  
Mahran Zeity ◽  
Nagappa Srinivas ◽  
Chinnamade Channegowde Gowda
Keyword(s):  
Spider Mite ◽  
Morphological Characters ◽  
Molecular Data ◽  
Mite Species ◽  
Coi Gene ◽  
High Similarity ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
High Degree ◽  
Degree Of Similarity

Study of morphological characters of Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara revealed high similarity by comparing all the important characters in addition to the characters pointed out by Ehara to separate those two species. Molecular phylogeny of seven Indian populations of T. macfarlanei and one population of T. malaysiensis from Philippines along with few distantly related species of Tetranychus was attempted. High degree of similarity between these two species at mitochondrial COI gene (96%) as well as ITS2 (rDNA) (96–99%) region was evident. Based on both morphological features and molecular data, T. malaysiensis is proposed as a junior synonym of T. macfarlanei based on ICZN’s law of priority. Also more female characters are prompted in this study to distinctly discriminate T. macfarlanei from its most resembling species, Tetranychus ludeni Zacher. Tetranychus macfarlanei has emerged as a pest of several cultivated crop plants in India. 

A new species of Cyrtodactylus (Squamata: Gekkonidae) from Ninh Thuan Province, southern Vietnam

Zootaxa ◽  
2021 ◽  
Vol 4999 (1) ◽  
pp. 58-76
Author(s):  
Quyen Hanh Do ◽  
TRUNG MY PHUNG ◽  
HANH THI NGO ◽  
MINH DUC LE ◽  
THOMAS ZIEGLER ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Coi Gene ◽  
Unique Combination ◽  
Mitochondrial Coi ◽  
Southern Vietnam ◽  
Mitochondrial Coi Gene ◽  
Morphological Differences ◽  
Dorsal Pattern ◽  
A New Species

A new species of the Cyrtodactylus irregularis group is described from Ninh Thuan Province, southern Vietnam based on molecular divergence and morphological differences. Cyrtodactylus orlovi sp. nov. is distinguished from the remaining Indochinese bent-toed geckos by having the unique combination of the following characters: size medium (SVL 61.0–77.7 mm); dorsal tubercles in 16–20 irregular rows; 36–39 ventral scale rows; precloacal pores absent in females, 5 or 6 in males, in a continuous row; femoral pores absent; 3–8 enlarged femoral scales; postcloacal spurs 1 or 2; lamellae under toe IV 16–19; a continuous neckband; a highly irregular transverse banded dorsal pattern; the absence of transversely enlarged median subcaudal scales. In phylogenetic analyses, the new species was revealed to be the sister taxon to a clade consisting of Cyrtodactylus cattienensis and the most recently described species from Vietnam, C. chungi, with 12.1–12.4% and 11.7 % pairwise genetic divergence from the two species, respectively, based on a fragment of the mitochondrial COI gene.  

A new species of Carpenter Moths (Lepidoptera, Cossidae) from Tarbagatai (NE Kazakhstan) and Altai (SW Siberia, Russia) Mountains

Zootaxa ◽  
2020 ◽  
Vol 4896 (1) ◽  
pp. 85-95
Author(s):  
ROMAN V. YAKOVLEV ◽  
NAZAR A. SHAPOVAL ◽  
VADIM V. IVONIN ◽  
SVYATOSLAV A. KNYAZEV ◽  
GALINA N. KUFTINA ◽  
...  
Keyword(s):  
New Species ◽  
Male Genitalia ◽  
Molecular Data ◽  
Coi Gene ◽  
Wing Pattern ◽  
Altai Mountains ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
A New Species

We described a new cossid species, Dyspessa ulgen sp. nov. from the Tarbagatai and Altai Mountains and compared it to other taxa of Dyspessa reported from the region (D. tristis, D. saldaitisi, D. saissanica), as well as to morphologically similar D. ulula. The new species is most closely related to D. ulula but differs from the latter in the characteristics of the male genitalia, wing pattern, and molecular data (a 658 bp fragment of the mitochondrial COI gene). 

Instant taxonomy: choosing adequate characters for species delimitation and description through congruence between molecular data and quantitative shape analysis

2018 ◽  
Vol 32 (3) ◽  
pp. 551 ◽  
Author(s):  
Tomislav Karanovic ◽  
Seunghan Lee ◽  
Wonchoel Lee
Keyword(s):  
Shape Analysis ◽  
Phenotypic Variability ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Coi Gene ◽  
Molecular Barcoding ◽  
Mitochondrial Coi ◽  
University Funding ◽  
Interspecific Differences ◽  
Northern Pacific

The lack of university funding is one of the major impediments to taxonomy, partly because traditional taxonomic training takes longer than a PhD course. Understanding ranges of phenotypic variability for different morphological structures, and their use as characters for delimitation and description of taxa, is a tedious task. We argue that the advent of molecular barcoding and quantitative shape analysis makes it unnecessary. As an example, we tackle a problematic species-complex of marine copepods from Korea and Japan, approaching it as a starting taxonomist might. Samples were collected from 14 locations and the mitochondrial COI gene was sequenced from 42 specimens. Our phylogenetic analyses reveal four distinct clades in Korea and Japan, and an additional nine belonging to a closely related complex from other parts of the Northern Pacific. Twenty different morphological structures were analysed for one Japanese and two Korean clades using landmark-based two-dimensional geometric morphometrics. Although there is no single morphological character that can distinguish with absolute certainty all three cryptic species, most show statistically significant interspecific differences in shape and size. We use five characters to describe two new species from Korea and to re-describe Tigriopus japonicus Mori, 1938 from near its type locality.

scholarly journals Reinertia, a New Subgenus of the Genus Aedes Meigen and Its Type Species Aedes (Reinertia) suffusus (Diptera: Culicidae), Newly Recorded From Bhutan

2021 ◽  
Author(s):  
Pradya Somboon ◽  
Thanari Phanitchakun ◽  
Jassada Saingamsook ◽  
Rinzin Namgay ◽  
Ralph E Harbach
Keyword(s):  
Male Genitalia ◽  
Type Species ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Molecular Data ◽  
Coi Gene ◽  
Maxillary Palpus ◽  
Mitochondrial Coi ◽  
New Subgenus ◽  
Tree Hole

Abstract A new subgenus, Reinertia Somboon, Namgay & Harbach, of the genus Aedes Meigen and its type species, Ae. suffusus Edwards, are described from specimens reared from larvae and pupae found in a tree hole in Bhutan. The scutum of the adults is mostly covered with narrow pale falcate scales. The proboscis, maxillary palpus, tibiae, and tarsi are dark-scaled. The gonocoxite of the male genitalia bears a unique setose basomesal sclerite. The larva closely resembles larvae of the subgenus Downsiomyia Vargus in having setae 4–6-C with numerous branches and inserted more or less on level with seta 7-C, abdominal seta 12-I is present and the comb is composed of 6–10 spine-like scales arranged in an irregular row. Surprisingly, Reinertia shares features of the adult habitus, male genitalia, and larva with the Palearctic subgenus Dahliana Reinert, Harbach & Kitching. However, in phylogenetic analyses of the mitochondrial COI gene of species representing 38 subgenera of Aedes and six other genera of the tribe Aedini Neveu-Lemaire, Reinertia was not associated with Dahliana or Downsiomyia. In both maximum likelihood and Bayesian analyses of the data, Ae. suffusus was recovered as the weakly supported sister of a clade composed of five species of the subgenus Protomacleaya Theobald. In the absence of strong support, and because Protomacleaya is an unnatural group of species that resemble each other phenetically by virtue of what they lack, Ae. suffusus cannot be placed in the subgenus Protomacleaya. Thus, the morphological and molecular data attest the uniqueness of Ae. suffusus and its recognition as a monobasic subgeneric lineage.

Fixation, description and DNA barcode of a neotype for Botryllus schlosseri (Pallas, 1766) (Tunicata, Ascidiacea)

Zootaxa ◽  
2017 ◽  
Vol 4353 (1) ◽  
pp. 29 ◽  
Author(s):  
RICCARDO BRUNETTI ◽  
LUCIA MANNI ◽  
FRANCESCO MASTROTOTARO ◽  
CARMELA GISSI ◽  
FABIO GASPARINI
Keyword(s):  
Developmental Stages ◽  
Dna Barcode ◽  
Molecular Data ◽  
Coi Gene ◽  
Morphological Description ◽  
Botryllus Schlosseri ◽  
Colonial Ascidian ◽  
History Museum ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

Botryllus schlosseri is a widespread colonial ascidian commonly considered cosmopolitan and amply used as model for researches ranging from developmental biology to immunobiology. Recently, molecular data lead to hypothesize that the species named B. schlosseri may consist of more than a single taxon. Indeed, five highly divergent clades, named A-E, have been genetically identified and are referred as cryptic species. In this context, and lacking both a type and a detailed morphological description, we believe that it is necessary, as a taxonomic reference point, to designate a neotype and re-describe the species. Therefore, a sample from the Lagoon of Venice (Adriatic Sea, Italy) was deposited as neotype in the Natural History Museum of Venice (Italy), preserved both in formalin and in 90% ethanol. Here we provide a morphological description of the suggested neotype of B. schlosseri that takes into account several developmental stages (oozooid, zooid of first blastogenic generations, and mature zooid) and is carefully compared with the previous descriptions of samples coming from other European and non-European localities. Finally, we associate our morphological description to a “DNA barcode”, consisting in a long fragment of the mitochondrial COI gene. Our description is associated to clade A, although at now we cannot guarantee that this association is univocal.

scholarly journals Taxonomy and phylogenetic relationships of the coral genera Australomussa and Parascolymia (Scleractinia, Lobophylliidae)

2014 ◽  
Vol 83 (3) ◽  
pp. 195-S7 ◽  
Author(s):  
Roberto Arrigoni ◽  
Zoe T. Richards ◽  
Chaolun Allen Chen ◽  
Andrew H. Baird ◽  
Francesca Benzoni
Keyword(s):  
Histone H3 ◽  
Taxonomic Status ◽  
Its Region ◽  
Molecular Data ◽  
Coi Gene ◽  
Phylogenetic Position ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
The Family ◽  
Micromorphological Characters

Novel micromorphological characters in combination with molecular studies have led to an extensive revision of the taxonomy and systematics of scleractinian corals. In the present work, we investigate the macro- and micromorphology and the phylogenetic position of the genera Australomussa and Parascolymia, two monotypic genera ascribed to the family Lobophylliidae. The molecular phylogeny of both genera was addressed using three markers, the partial mitochondrial COI gene and the nuclear histone H3 and the ribosomal ITS region. Based on molecular data, Australomussa and Parascolymia belong to the Lobophylliidae and they cluster together with the genera Lobophyllia and Symphyllia within the same clade. While A. rowleyensis and P. vitiensis are closely related based on the three gene regions examined, their macro and micromorphology suggest that these species are distinct, differing in several characters, such as continuity and thickness of the costosepta, the number of septa, septal tooth height, spacing, and shape, and the distribution and shape of granules. Thus, we revise the taxonomic status of the genus Australomussa as a junior synonym of Parascolymia.

Identification of a species of deer fly attacking human and live stocks in Assam, India

ENTOMON ◽  
2019 ◽  
Vol 44 (3) ◽  
pp. 237-240
Author(s):  
Dipankar Biswas ◽  
Dibya Ranjan Bhattacharyya ◽  
Kaushal Yadav ◽  
Pranjal Jyoti Baruah ◽  
Biswa Jyoti Borkakoty
Keyword(s):  
Present Article ◽  
Sequence Data ◽  
Molecular Data ◽  
Northeast India ◽  
Species Level ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Preliminary Examination ◽  
Mitochondrial Coi Gene ◽  
Sudden Appearance

During April 2018, a sudden appearance of some unknown flies was observed in some villages under Sibsagar district of Assam, Northeast India. The flies attacked in groups and had more attraction towards human than livestock. The present article describes the entomological and molecular identification of the fly at species level in Sibsagar district of Assam, northeast India. Preliminary examination revealed the fly to be an insect of order Diptera, Family Tabanidae and Genus Chrysops as they posses short proboscis, ocelli, third antennal joint with five divisions and wings demarcated with dark median cross-band. This was supported by molecular data where the partial nucleotide sequences of Mitochondrial COI gene revealed maximum identity (90.6%-92.3%) with genus Chrysops. The mitochondrial COI sequence data of Chrysops flavocinctus has been made available in NCBI Gen Bank. Gen Bank Accession No. MH998226.

scholarly journals Just the once will not hurt: DNA suggests species lumping over two oceans in deep-sea snails (Cryptogemma)

2020 ◽  
Vol 190 (2) ◽  
pp. 532-557 ◽  
Author(s):  
Paul Zaharias ◽  
Yuri I Kantor ◽  
Alexander E Fedosov ◽  
Francesco Criscione ◽  
Anders Hallan ◽  
...  
Keyword(s):  
Deep Sea ◽  
Species Delimitation ◽  
Molecular Data ◽  
Shell Morphology ◽  
Coi Gene ◽  
Type Specimens ◽  
Mitochondrial Coi ◽  
Species Complexes ◽  
Mitochondrial Coi Gene ◽  
Taxonomic Studies

Abstract The practice of species delimitation using molecular data commonly leads to the revealing of species complexes and an increase in the number of delimited species. In a few instances, however, DNA-based taxonomy has led to lumping together of previously described species. Here, we delimit species in the genus Cryptogemma (Gastropoda: Conoidea: Turridae), a group of deep-sea snails with a wide geographical distribution, primarily by using the mitochondrial COI gene. Three approaches of species delimitation (ABGD, mPTP and GMYC) were applied to define species partitions. All approaches resulted in eight species. According to previous taxonomic studies and shell morphology, 23 available names potentially apply to the eight Cryptogemma species that were recognized herein. Shell morphometrics, radular characters and geographical and bathymetric distributions were used to link type specimens to these delimited species. In all, 23 of these available names are here attributed to seven species, resulting in 16 synonymizations, and one species is described as new: Cryptogemma powelli sp. nov. We discuss the possible reasons underlying the apparent overdescription of species within Cryptogemma, which is shown here to constitute a rare case of DNA-based species lumping in the hyper-diversified superfamily Conoidea.

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