Changes in optical properties of tissue during acute wound healing in an animal model

Diabetic foot ulcers are a common cause of morbidity in diabetic patients. One of the main pathogens found in these ulcers is methicillin-resistant Staphylococcus aureus (MRSA). MRSA often carries resistance to several classes of antibiotics and their infections are becoming harder to treat. Therefore, new alternatives are urgently needed. Thus, this study aimed to investigate the capacity of topical ozonated oil application on the treatment of early-stage skin infected with MRSA in an animal model. Ozonated oil was prepared from a mixture of oils subjected to a gas stream of O2/O3 mixture. Sixteen Wistar rats were inoculated by an intradermic injection of MRSA suspension, producing an abscess lesion. After 3 days, the skin epidermis was removed to open the wound. Group 1 received an application of oil mixture without ozone treatment and Group 2 received an application of ozonated oil. After the treatment period, skin was collected, colony-forming units (CFU) of bacteria were quantified and the histological analysis of the skin was carried out. Skin samples from the control 1 and 2 had a bacterial load was of 1.1 × 105 and 5.7 × 103 CFU/mL, respectively. Group 2 showed better wound healing from mild to moderate epidermal regeneration. Topical application of ozonated vegetable oil in MRSA-infected skin in rats showed a small reduction of the bacterial load and better wound healing.

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Monitoring of Acute Wound Healing

10.1364/biomed.2008.pdpbsue1 ◽

2008 ◽

Author(s):

Elisabeth S. Papazoglou ◽

Michael S. Weingarten ◽

Leonid Zubkov ◽

Michael Neidrauer ◽

Linda Zhu ◽

...

Keyword(s):

Wound Healing ◽

Acute Wound

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An in vivo Investigation on the Wound-Healing Effect of Two Medicinal Herbs Using an Animal Model with Foot Ulcer

European Surgical Research ◽

10.1159/000122834 ◽

2008 ◽

Vol 41 (1) ◽

pp. 15-23 ◽

Cited By ~ 45

Author(s):

T.W. Lau ◽

D.S. Sahota ◽

C.H. Lau ◽

C.M. Chan ◽

F.C. Lam ◽

...

Keyword(s):

Wound Healing ◽

Animal Model ◽

Foot Ulcer ◽

Medicinal Herbs ◽

Healing Effect ◽

Wound Healing Effect

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Quantitative estimate of spatial/temporal variations in the optical properties of tissue-like media from diffuse reflectance data

10.1117/12.478167 ◽

2003 ◽

Author(s):

Francesco Fabbri ◽

Maria-Angela Franceschini ◽

Sergio Fantini

Keyword(s):

Optical Properties ◽

Quantitative Estimate ◽

Diffuse Reflectance ◽

Temporal Variations ◽

Optical Properties Of Tissue ◽

Reflectance Data

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Transplantation of Bone Marrow Cells Preactivated With Sodium Nitroprusside Improves Acute Wound Healing in Rabbits

The International Journal of Lower Extremity Wounds ◽

10.1177/15347346211029078 ◽

2021 ◽

pp. 153473462110290

Author(s):

Nazira Fatima ◽

Muhammad Saleem

Keyword(s):

Bone Marrow ◽

Wound Healing ◽

Sodium Nitroprusside ◽

Bone Marrow Cells ◽

Healing Process ◽

Wound Healing Process ◽

Inflammatory Infiltration ◽

Acute Wound ◽

High Concentrations ◽

Marrow Cells

The development of wound healing impairment mainly represents challenging clinical problems. The less and high concentrations of nitric oxide can influence angiogenesis, remodeling, and proliferation of skin cells. Delayed acute wounds generally have failed to progress via the normal stages of healing. Such wounds usually enter a state of pathological inflammation due to a postponed, incomplete, and uncoordinated healing process. This study aimed to investigate the effect of normal bone marrow cells (BMCs) and preconditioning of BMCs with minimum concentrations of sodium nitroprusside (NaNP) solution for acute wound healing. For acute wound healing, full-thickness dorsal wounds were created on rabbits. The acute wound of rabbits was treated with BMCs and preactivated BMCs with NaNP. Histological results showed that BMCs preactivated with NaNP could improve collagen deposition, enhanced reepithelization, and decreased inflammatory infiltration. Overall, BMCs treated with NaNP can help to improve acute wound healing in rabbits. The result strongly confirmed the beneficial effect in augmenting the wound healing process. The combination of BMCs with NaNP was safe and convenient for acute wound healing.

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