Transplantation of Bone Marrow Cells Preactivated With Sodium Nitroprusside Improves Acute Wound Healing in Rabbits

2021 ◽  
pp. 153473462110290
Author(s):  
Nazira Fatima ◽  
Muhammad Saleem
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Sodium Nitroprusside ◽  
Bone Marrow Cells ◽  
Healing Process ◽  
Wound Healing Process ◽  
Inflammatory Infiltration ◽  
Acute Wound ◽  
High Concentrations ◽  
Marrow Cells

The development of wound healing impairment mainly represents challenging clinical problems. The less and high concentrations of nitric oxide can influence angiogenesis, remodeling, and proliferation of skin cells. Delayed acute wounds generally have failed to progress via the normal stages of healing. Such wounds usually enter a state of pathological inflammation due to a postponed, incomplete, and uncoordinated healing process. This study aimed to investigate the effect of normal bone marrow cells (BMCs) and preconditioning of BMCs with minimum concentrations of sodium nitroprusside (NaNP) solution for acute wound healing. For acute wound healing, full-thickness dorsal wounds were created on rabbits. The acute wound of rabbits was treated with BMCs and preactivated BMCs with NaNP. Histological results showed that BMCs preactivated with NaNP could improve collagen deposition, enhanced reepithelization, and decreased inflammatory infiltration. Overall, BMCs treated with NaNP can help to improve acute wound healing in rabbits. The result strongly confirmed the beneficial effect in augmenting the wound healing process. The combination of BMCs with NaNP was safe and convenient for acute wound healing.

scholarly journals Effects of neuraminidase on the regulation of erythropoiesis

Blood ◽  
1984 ◽  
Vol 63 (4) ◽  
pp. 784-788 ◽  
Author(s):  
VF LaRussa ◽  
F Sieber ◽  
LL Sensenbrenner ◽  
SJ Sharkis
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Colony Growth ◽  
Mouse Bone Marrow ◽  
Continuous Exposure ◽  
Low Concentrations ◽  
High Concentrations ◽  
Marrow Cells

Abstract In this article, we present evidence that sialic acid-containing surface components play a role in the regulation of erythropoiesis. A 1- hr exposure of mouse bone marrow cells to high concentrations of neuraminidase reduced erythroid colony formation. Coculture of 10(6) untreated thymocytes with neuraminidase-treated bone marrow cells restored erythroid colony growth. Neuraminidase-treated thymocytes retained their ability to suppress erythroid colony formation by untreated marrow cells, but lost their ability to enhance erythroid colony formation. Continuous exposure to low concentrations of neuraminidase enhanced erythroid bone marrow cell colony growth in response to a suboptimal dose of erythropoietin.

scholarly journals Fish Collagen Surgical Compress Repairing Characteristics on Wound Healing Process In Vivo

Marine Drugs ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. 33 ◽  
Author(s):  
Jingjing Chen ◽  
Kaili Gao ◽  
Shu Liu ◽  
Shujun Wang ◽  
Jeevithan Elango ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Collagen Sponge ◽  
Wound Healing Process ◽  
Inflammatory Infiltration ◽  
Accelerate Wound Healing ◽  
Soluble Collagen ◽  
Bovine Collagen ◽  
Lower Effect

The development of biomaterials with the potential to accelerate wound healing is a great challenge in biomedicine. In this study, four types of samples including pepsin soluble collagen sponge (PCS), acid soluble collagen sponge (ACS), bovine collagen electrospun I (BCE I) and bovine collagen electrospun II (BCE II) were used as wound dressing materials. We showed that the PCS, ACS, BCE I and BCE II treated rats increased the percentage of wound contraction, reduced the inflammatory infiltration, and accelerated the epithelization and healing. PCS, ACS, BCE I, and BCE II significantly enhanced the total protein and hydroxyproline level in rats. ACS could induce more fibroblasts proliferation and differentiation than PCS, however, both PCS and ACS had a lower effect than BCE I and BCE II. PCS, ACS, BCE I, and BCE II could regulate deposition of collagen, which led to excellent alignment in the wound healing process. There were similar effects on inducing the level of cytokines including EGF, FGF, and vascular endothelial marker CD31 among these four groups. Accordingly, this study disclosed that collagens (PCS and ACS) from tilapia skin and bovine collagen electrospun (BCE I and BCE II) have significant bioactivity and could accelerate wound healing rapidly and effectively in rat model.

scholarly journals Expression of Shelterin ComponentPOT1Is Associated with Decreased Telomere Length and Immunity Condition in Humans with Severe Aplastic Anemia

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Ting Wang ◽  
Shu-chong Mei ◽  
Rong Fu ◽  
Hua-quan Wang ◽  
Zong-hong Shao
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Telomere Length ◽  
Bone Marrow Cells ◽  
Culture Media ◽  
Telomere Attrition ◽  
High Concentrations ◽  
Relationship Of ◽  
The Relationship ◽  
Marrow Cells

Abnormal telomere attrition has been found to be closely related to patients with SAA in recent years. To identify the incidence of telomere attrition in SAA patients and investigate the relationship of telomere length with clinical parameters, SAA patients(n=27)and healthy controls(n=15)were enrolled in this study. Telomere length of PWBCs was significantly shorter in SAA patients than in controls. Analysis of gene expression of Shelterin complex revealed markedly low levels ofPOT1expression in SAA groups relative to controls. No differences in the gene expression of the other Shelterin components—TRF1,TRF2,TIN2,TPP1, andRAP1—were identified. Addition of IFN-γto culture media induced a similar fall in POT1 expression in bone marrow cells to that observed in cells cultured in the presence of SAA serum, suggesting IFN-γis the agent responsible for this effect of SAA serum. Furthermore, ATR, phosphorylated ATR, and phosphorylated ATM/ATR substrate were all found similarly increased in bone marrow cells exposed to SAA serum, TNF-α, or IFN-γ. In summary, SAA patients have short telomeres and decreased POT1 expression. TNF-αand IFN-γare found at high concentrations in SAA patients and may be the effectors that trigger apoptosis through POT1 and ATR.

scholarly journals EFFICIENCY OF SYSTEMIC VERSUS INTRALESIONAL INJECTION OF BONE MARROW CELLS ON BUCCAL WOUND HEALING IN DIABETIC RATS.

2017 ◽  
Vol 5 (3) ◽  
pp. 202-212
Author(s):  
FatmaIbrahim Elfaiedi ◽  
◽  
MonaE. Denewar ◽  
FatmaM.M. Ibrahim. ◽  
◽  
...  
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Bone Marrow Cells ◽  
Diabetic Rats ◽  
Intralesional Injection ◽  
Marrow Cells

scholarly journals Hubungan Status Gizi dengan Proses Penyembuhan Luka pada Pasien Post Apendektomi

2020 ◽  
Vol 1 (3) ◽  
pp. 226-232
Author(s):  
Andi Siswandi ◽  
Mardheni Wulandari ◽  
Mizar Erianto ◽  
Azahrah Mawaddah Noviska
Keyword(s):  
Wound Healing ◽  
Nutritional Status ◽  
Chronic Wounds ◽  
Healing Process ◽  
Wound Healing Process ◽  
Chi Square ◽  
Cross Sectional ◽  
Surgical Infection ◽  
Chi Square Test ◽  
Acute Wound

Appendicitis is part of the emergency cases that often occur in the abdominal area. Appendicitis is a prototype disease that takes place through inflammation due to obstruction and ischemia with the main symptoms which is pain that reflects the state of the disease. Appendicitis requires surgical appendectomy to reduce the risk of perforation. Apendectomy surgery is one type of wound that is an incision wound. The time of wound healing can be determined by the distinguishing between types of acute or chronic wounds. Appendectomy that does not have a post-surgical infection is categorized as an acute wound, physiologically the acute wound will heal ± 0-21 days. However, if the provision of nutrition is not fulfilled properly it will be almost the wound healing process. One factor that can influence the wound healing process is nutritional status. This study aim to determine the correlation of nutritional status with the wound healing process of post-appendectomy patients in RSUD Dr. H. Abdoel Moeloek, Bandar Lampung. This study used observational analytic with cross sectional approach. The sampling technique used was accidental sampling. The data were analyzed by using Chi-square test. From the Chi-square test found a relationship of nutritional status with a significant wound healing process (p <0.05) which is obtained p = 0.004, and the value obtained (OR = 10.5) can be said that adequate nutritional status is 10.5 times more likely to experience good wound healing when compared to undernourished nutritional status. There is a correlation between nutritional status and wound healing process in post-appendectomy patients.

scholarly journals LeprdbDiabetic Mouse Bone Marrow Cells Inhibit Skin Wound Vascularization but Promote Wound Healing

2003 ◽  
Vol 92 (11) ◽  
pp. 1247-1253 ◽  
Author(s):  
Vesna Stepanovic ◽  
Ola Awad ◽  
Chunhua Jiao ◽  
Martine Dunnwald ◽  
Gina C. Schatteman
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Bone Marrow Cells ◽  
Skin Wound ◽  
Mouse Bone Marrow ◽  
Promote Wound Healing ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells

scholarly journals Nitric Oxide Produced by the Antioxidant Activity of Verapamil Improves the Acute Wound Healing Process

2021 ◽  
Vol 18 (1) ◽  
pp. 179-186
Author(s):  
Yu Na Han ◽  
Yoon Jae Lee ◽  
Ki Joo Kim ◽  
Su Jin Lee ◽  
Jang Youn Choi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Wound Healing ◽  
Healing Process ◽  
Wound Healing Process ◽  
Acute Wound

scholarly journals Effects of neuraminidase on the regulation of erythropoiesis

Blood ◽  
1984 ◽  
Vol 63 (4) ◽  
pp. 784-788
Author(s):  
VF LaRussa ◽  
F Sieber ◽  
LL Sensenbrenner ◽  
SJ Sharkis
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Colony Growth ◽  
Mouse Bone Marrow ◽  
Continuous Exposure ◽  
Low Concentrations ◽  
High Concentrations ◽  
Marrow Cells

In this article, we present evidence that sialic acid-containing surface components play a role in the regulation of erythropoiesis. A 1- hr exposure of mouse bone marrow cells to high concentrations of neuraminidase reduced erythroid colony formation. Coculture of 10(6) untreated thymocytes with neuraminidase-treated bone marrow cells restored erythroid colony growth. Neuraminidase-treated thymocytes retained their ability to suppress erythroid colony formation by untreated marrow cells, but lost their ability to enhance erythroid colony formation. Continuous exposure to low concentrations of neuraminidase enhanced erythroid bone marrow cell colony growth in response to a suboptimal dose of erythropoietin.

Sign in / Sign up

Export Citation Format

Share Document