scholarly journals Nonscanning large-area Raman imaging for ex vivo/in vivo skin cancer discrimination

2018 ◽  
Vol 23 (10) ◽  
pp. 1 ◽  
Author(s):  
Elmar Schmälzlin ◽  
Benito Moralejo ◽  
Ingo Gersonde ◽  
Johannes Schleusener ◽  
Maxim E. Darvin ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Ex Vivo ◽  
Raman Imaging ◽  
Large Area

scholarly journals FLAME: Macroscopic imaging with microscopic resolution. Optical biopsy of human skin

2020 ◽  
Author(s):  
Alexander Fast ◽  
Akarsh Lal ◽  
Amanda F. Durkin ◽  
Christopher B. Zachary ◽  
Anand K. Ganesan ◽  
...  
Keyword(s):  
Human Skin ◽  
Single Photon ◽  
Ex Vivo ◽  
Photon Counting ◽  
Optical Biopsy ◽  
Large Area ◽  
Time Resolved ◽  
Distribution Maps ◽  
Imaging Tool

AbstractWe introduce a compact, fast large area multiphoton exoscope (FLAME) system with enhanced molecular contrast for macroscopic imaging of human skin with microscopic resolution. A versatile imaging platform with multiple modes of operation for comprehensive analysis of live or resected thick human skin tissue, it produces 3D images that encompass sub-mm2 to cm2 scale areas of tissue within minutes. The FLAME imaging platform, which expands on a design recently introduced by our group, features deep learning, additional scanning hardware elements and time-resolved single photon counting detection to uniquely allow fast discrimination and 3D virtual staining of melanin. We demonstrate its performance and utility by fast ex vivo and in vivo imaging of human skin. With the ability to provide rapid access to depth resolved images of skin over cm2 area and to generate 3D distribution maps of key sub-cellular skin components such as melanocytic dendrites and melanin, FLAME represents a promising imaging tool for enhancing diagnosis accuracy, guiding therapy and understanding skin biology.

scholarly journals Nobiletin-loaded composite penetration enhancer vesicles restore the normal miRNA expression and the chief defence antioxidant levels in skin cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mahitab Bayoumi ◽  
Mona G. Arafa ◽  
Maha Nasr ◽  
Omaima A. Sammour
Keyword(s):  
Particle Size ◽  
Skin Cancer ◽  
Zeta Potential ◽  
Ex Vivo ◽  
Physical Stability ◽  
Entrapment Efficiency ◽  
Penetration Enhancer ◽  
Oxidative Stress Biomarkers ◽  
Skin Deposition

AbstractSkin cancer is one of the most dangerous diseases, leading to massive losses and high death rates worldwide. Topical delivery of nutraceuticals is considered a suitable approach for efficient and safe treatment of skin cancer. Nobiletin; a flavone occurring in citrus fruits has been reported to inhibit proliferation of carcinogenesis since 1990s, is a promising candidate in this regard. Nobiletin was loaded in various vesicular systems to improve its cytotoxicity against skin cancer. Vesicles were prepared using the thin film hydration method, and characterized for particle size, zeta potential, entrapment efficiency, TEM, ex-vivo skin deposition and physical stability. Nobiletin-loaded composite penetration enhancer vesicles (PEVs) and composite transfersomes exhibited particle size 126.70 ± 11.80 nm, 110.10 ± 0.90 nm, zeta potential + 6.10 ± 0.40 mV, + 9.80 ± 2.60 mV, entrapment efficiency 93.50% ± 3.60, 95.60% ± 1.50 and total skin deposition 95.30% ± 3.40, 100.00% ± 2.80, respectively. These formulations were selected for cytotoxicity study on epidermoid carcinoma cell line (A431). Nobiletin-loaded composite PEVs displayed the lowest IC50 value, thus was selected for the in vivo study, where it restored skin condition in DMBA induced skin carcinogenesis mice, as delineated by histological and immuno-histochemical analysis, biochemical assessment of skin oxidative stress biomarkers, in addition to miRNA21 and miRNA29A. The outcomes confirmed that nobiletin- loaded composite PEVs is an efficient delivery system combating skin cancer.

scholarly journals Noninvasive and Highly Multiplexed Five-Color Tumor Imaging of Near-Infrared Resonant Surface-Enhanced Raman Nanoparticles In Vivo

2020 ◽  
Author(s):  
Jung-Ho Yu ◽  
Idan Steinberg ◽  
Ryan Miller Davis ◽  
Andrey V. Malkovskiy ◽  
Aimen Zlitni ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tumor Imaging ◽  
Ex Vivo ◽  
Spectral Width ◽  
Raman Imaging ◽  
Multiple Tumor ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Longitudinal Imaging

In vivo multiplexed imaging aims for noninvasive analysis of multiple tumor biomarkers. While most of the preclinical imaging has provided a number of multiplexing channels up to three, Raman imaging with surface-enhanced Raman scattering (SERS) nanoparticles was suggested to offer higher multiplexing capability originating from their narrow spectral width. However, the multiplexed Raman imaging is still in its infancy for visualizing tumors with both sufficient multiplicity and high sensitivity concurrently. Here we create multispectral palettes of gold core-near-infrared (NIR) resonant Raman dyes-silica shell SERS (NIR-SERRS) nanoparticles, which provide both the high multiplicity and fluorescence-comparable sensitivity. With the NIR-SERRS nanoparticle palettes, we demonstrate noninvasive and five-plex ratiometric Raman imaging of tumors in living mice. Furthermore, we perform noninvasive and longitudinal imaging of the five-color nanoparticles in the tumors, which is not feasible with current ex vivo multiplexing platforms, demonstrating great potential for noninvasive assessment of multiple biological targets within tumors.

A Miniature Robotic-Assisted Tool for Large Area Endomicroscopy Scanning

2020 ◽  
Vol 12 (3) ◽  
Author(s):  
Haibo Wang ◽  
Derui Kong ◽  
Siyang Zuo
Keyword(s):  
Ex Vivo ◽  
Human Thyroid ◽  
Margin Assessment ◽  
Large Area ◽  
Surgical Guidance ◽  
Tissue Identification ◽  
Recent Developments ◽  
Thyroid Gland Tissue ◽  
Small Field Of View

Abstract Recent developments in probe-based optical imaging tools such as confocal endomicroscopy have been demonstrated to be powerful techniques that can provide high-resolution in vivo morphological images at cellular and subcellular levels. Such tools may aid in real-time assessment of cancerous tissue and can also be used for intraoperative surgical guidance. However, it is a challenge to scan over a large area due to the small field-of-view and the difficulties associated with manually manipulating the probe. In this paper, a hand-held miniature scanning device for controlled, large area imaging is proposed. The device can scan target surfaces automatically. A passive linear mechanism is used to maintain good probe–tissue contacts. The results demonstrated that large area mosaics up to 31.4 mm2 can be obtained. The experimental results for ex vivo human thyroid gland tissue have shown the potential of the device for improving the prospects of intraoperative tissue identification and margin assessment.

scholarly journals The Contribution of Electron Paramagnetic Resonance to Melanoma Research

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Quentin Godechal ◽  
Bernard Gallez
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Skin Cancer ◽  
Ex Vivo ◽  
Current Method ◽  
Basic Knowledge ◽  
Ultraviolet Rays ◽  
Paramagnetic Resonance ◽  
Endogenous Substrate ◽  
Electron Paramagnetic

The incidence of malignant melanoma, the most dangerous form of skin cancer, is rising each year. However, some aspects of the tumor initiation and development are still unclear, and the current method of diagnosis, based on the visual aspect of the tumor, shows limitations. For these reasons, developments of new techniques are ongoing to improve basic knowledge on the disease and diagnosis of tumors in individual patients. This paper shows how electron paramagnetic resonance (EPR), a method able to detect free radicals trapped in melanin pigments, has recently brought its unique value to this specific field. The general principles of the method and the convenience of melanin as an endogenous substrate for EPR measurements are explained. Then, the way by which EPR has recently helped to assess the contribution of ultraviolet rays (UVA and UVB) to the initiation of melanoma is described. Finally, we describe the improvements of EPR spectrometry and imaging in the detection and mapping of melanin pigments insideex vivoandin vivomelanomas. We discuss how these advances might improve the diagnosis of this skin cancer and point out the present capabilities and limitations of the method.

scholarly journals Fast, large area multiphoton exoscope (FLAME) for macroscopic imaging with microscopic resolution of human skin

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Alexander Fast ◽  
Akarsh Lal ◽  
Amanda F. Durkin ◽  
Griffin Lentsch ◽  
Ronald M. Harris ◽  
...  
Keyword(s):  
Human Skin ◽  
Single Photon ◽  
Ex Vivo ◽  
Photon Counting ◽  
Large Area ◽  
Time Resolved ◽  
Distribution Maps ◽  
Imaging Tool ◽  
Molecular Contrast

Abstract We introduce a compact, fast large area multiphoton exoscope (FLAME) system with enhanced molecular contrast for macroscopic imaging of human skin with microscopic resolution. A versatile imaging platform, FLAME combines optical and mechanical scanning mechanisms with deep learning image restoration to produce depth-resolved images that encompass sub-mm2 to cm2 scale areas of tissue within minutes and provide means for a comprehensive analysis of live or resected thick human skin tissue. The FLAME imaging platform, which expands on a design recently introduced by our group, also features time-resolved single photon counting detection to uniquely allow fast discrimination and 3D virtual staining of melanin. We demonstrate its performance and utility by fast ex vivo and in vivo imaging of human skin. With the ability to provide rapid access to depth resolved images of skin over cm2 area and to generate 3D distribution maps of key sub-cellular skin components such as melanocytic dendrites and melanin, FLAME is ready to be translated into a clinical imaging tool for enhancing diagnosis accuracy, guiding therapy and understanding skin biology.

scholarly journals Noninvasive and Highly Multiplexed Five-Color Tumor Imaging of Near-Infrared Resonant Surface-Enhanced Raman Nanoparticles In Vivo

2020 ◽  
Author(s):  
Jung-Ho Yu ◽  
Idan Steinberg ◽  
Ryan Miller Davis ◽  
Andrey V. Malkovskiy ◽  
Aimen Zlitni ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tumor Imaging ◽  
Ex Vivo ◽  
Spectral Width ◽  
Raman Imaging ◽  
Multiple Tumor ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Longitudinal Imaging

In vivo multiplexed imaging aims for noninvasive analysis of multiple tumor biomarkers. While most of the preclinical imaging has provided a number of multiplexing channels up to three, Raman imaging with surface-enhanced Raman scattering (SERS) nanoparticles was suggested to offer higher multiplexing capability originating from their narrow spectral width. However, the multiplexed Raman imaging is still in its infancy for visualizing tumors with both sufficient multiplicity and high sensitivity concurrently. Here we create multispectral palettes of gold core-near-infrared (NIR) resonant Raman dyes-silica shell SERS (NIR-SERRS) nanoparticles, which provide both the high multiplicity and fluorescence-comparable sensitivity. With the NIR-SERRS nanoparticle palettes, we demonstrate noninvasive and five-plex ratiometric Raman imaging of tumors in living mice. Furthermore, we perform noninvasive and longitudinal imaging of the five-color nanoparticles in the tumors, which is not feasible with current ex vivo multiplexing platforms, demonstrating great potential for noninvasive assessment of multiple biological targets within tumors.

Discrimination of non-melanoma skin cancer and keratosis from normal skin tissue in vivo and ex vivo by Raman spectroscopy

2019 ◽  
Vol 100 ◽  
pp. 131-141 ◽  
Author(s):  
Ana Mara Ferreira Lima ◽  
Camila Ribeiro Daniel ◽  
Ricardo Scarparo Navarro ◽  
Benito Bodanese ◽  
Carlos Augusto Pasqualucci ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Skin Cancer ◽  
Ex Vivo ◽  
Normal Skin ◽  
Skin Tissue ◽  
Non Melanoma Skin Cancer ◽  
Melanoma Skin

The Role of Polyphenols in the Modulation of Plasma Non-Enzymatic Antioxidant Capacity (NEAC)

2012 ◽  
Vol 82 (3) ◽  
pp. 228-232 ◽  
Author(s):  
Mauro Serafini ◽  
Giuseppa Morabito
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Intervention ◽  
Ex Vivo ◽  
The Body ◽  
Dietary Polyphenols ◽  
Enzymatic Antioxidant ◽  
Endogenous Antioxidant

Dietary polyphenols have been shown to scavenge free radicals, modulating cellular redox transcription factors in different in vitro and ex vivo models. Dietary intervention studies have shown that consumption of plant foods modulates plasma Non-Enzymatic Antioxidant Capacity (NEAC), a biomarker of the endogenous antioxidant network, in human subjects. However, the identification of the molecules responsible for this effect are yet to be obtained and evidences of an antioxidant in vivo action of polyphenols are conflicting. There is a clear discrepancy between polyphenols (PP) concentration in body fluids and the extent of increase of plasma NEAC. The low degree of absorption and the extensive metabolism of PP within the body have raised questions about their contribution to the endogenous antioxidant network. This work will discuss the role of polyphenols from galenic preparation, food extracts, and selected dietary sources as modulators of plasma NEAC in humans.

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