Quantitative cross-polarization OCT image analysis of ex vivo human brain tissues and its comparison with MRI and histological data (Conference Presentation)

Author(s):  
Elena B. Kiseleva ◽  
Alexander A. Moiseev ◽  
Konstantin S. Yashin ◽  
Sergey S. Kuznetsov ◽  
Igor A. Medyanik ◽  
...  
2002 ◽  
Vol 47 (12) ◽  
pp. 2059-2073 ◽  
Author(s):  
A N Yaroslavsky ◽  
P C Schulze ◽  
I V Yaroslavsky ◽  
R Schober ◽  
F Ulrich ◽  
...  

2018 ◽  
Vol 318 (3) ◽  
pp. 2313-2319
Author(s):  
Ján Pánik ◽  
Martin Kopáni ◽  
Jakub Zeman ◽  
Miroslav Ješkovský ◽  
Jakub Kaizer ◽  
...  

Author(s):  
Alastair J Kirby ◽  
José P Lavrador ◽  
Istvan Bodi ◽  
Francesco Vergani ◽  
Ranjeev Bhangoo ◽  
...  

Abstract Background Lower-grade gliomas may be indolent for many years before developing malignant behaviour. The reasons mechanisms underlying malignant progression remain unclear. Methods We collected blocks of live human brain tissue donated by people undergoing glioma resection. The tissue blocks extended through the peritumoral cortex and into the glioma. The living human brain tissue was cut into ex vivo brain slices and bathed in 5-aminolevulinic acid (5-ALA). High-grade glioma cells avidly take up 5-aminolevulinic acid (5-ALA) and accumulate high levels of the fluorescent metabolite, Protoporphyrin IX (PpIX). We exploited the PpIX fluorescence emitted by higher-grade glioma cells to investigate the earliest stages of malignant progression in lower-grade gliomas. Results We found sparsely-distributed ‘hot-spots’ of PpIX-positive cells in living lower-grade glioma tissue. Glioma cells and endothelial cells formed part of the PpIX hotspots. Glioma cells in PpIX hotspots were IDH1 mutant and expressed nestin suggesting they had acquired stem-like properties. Spatial analysis with 5-ALA conjugated quantum dots indicated that these glioma cells replicated adjacent to blood vessels. PpIX hotspots formed in the absence of angiogenesis. Conclusion Our data show that PpIX hotspots represent microdomains of cells with high-grade potential within lower-grade gliomas and identify locations where malignant progression could start.


Author(s):  
Guzel R. Musina ◽  
Nikita V. Chernomyrdin ◽  
Irina N. Dolganova ◽  
Vladimir N. Kurlov ◽  
Pavel V. Nikitin ◽  
...  

2021 ◽  
Author(s):  
Adrit Rao ◽  
Harvey A. Fishman

Identifying diseases in Optical Coherence Tomography (OCT) images using Deep Learning models and methods is emerging as a powerful technique to enhance clinical diagnosis. Identifying macular diseases in the eye at an early stage and preventing misdiagnosis is crucial. The current methods developed for OCT image analysis have not yet been integrated into an accessible form-factor that can be utilized in a real-life scenario by Ophthalmologists. Additionally, current methods do not employ robust multiple metric feedback. This paper proposes a highly accurate smartphone-based Deep Learning system, OCTAI, that allows a user to take an OCT picture and receive real-time feedback through on-device inference. OCTAI analyzes the input OCT image in three different ways: (1) full image analysis, (2) quadrant based analysis, and (3) disease detection based analysis. With these three analysis methods, along with an Ophthalmologist's interpretation, a robust diagnosis can potentially be made. The ultimate goal of OCTAI is to assist Ophthalmologists in making a diagnosis through a digital second opinion and enabling them to cross-check their diagnosis before making a decision based on purely manual analysis of OCT images. OCTAI has the potential to allow Ophthalmologists to improve their diagnosis and may reduce misdiagnosis rates, leading to faster treatment of diseases.


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