Fluorescence lifetime imaging system with nm-resolution and single-molecule sensitivity

We developed a combined procedure for the analysis of works of art based on a portable system for fluorescence imaging integrated with analytical measurements on microsamples. The method allows us to localize and identify organic and inorganic compounds present on the surface of artworks. The fluorescence apparatus measures the temporal and spectral features of the fluorescence emission, excited by ultraviolet (UV) laser pulses. The kinetic of the emission is studied through a fluorescence lifetime imaging system, while an optical multichannel analyzer measures the fluorescence spectra of selected points. The chemical characterization of the compounds present on the artistic surfaces is then performed by means of analytical measurements on microsamples collected with the assistance of the fluorescence maps. The previous concepts have been successfully applied to study the contaminants on the surface of Michelangelo's David. The fluorescence analysis combined with Fourier transform infrared (FT-IR) measurements revealed the presence of beeswax, which permeates most of the statue surface, and calcium oxalate deposits mainly arranged in vertical patterns and related to rain washing.

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All-solid-state diode-pumped fluorescence lifetime imaging system for biomedicine and microscopy

10.1364/bio.1999.jtua4 ◽

1999 ◽

Author(s):

M. J. Cole ◽

K. Dowling ◽

R. Jones ◽

D. Parsons-Karavassilis ◽

P. M. W. French ◽

...

Keyword(s):

Solid State ◽

Fluorescence Lifetime ◽

Imaging System ◽

Fluorescence Lifetime Imaging ◽

Lifetime Imaging ◽

Diode Pumped

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Simultaneous Anatomical and Biochemical Imaging of Biological Tissue using a Multimodal Optical Coherence Tomography and Fluorescence Lifetime Imaging System

Biomedical Optics and 3-D Imaging ◽

10.1364/biomed.2010.bsuf4 ◽

2010 ◽

Author(s):

Sebina Shrestha ◽

Jesung Park ◽

Paritosh Pande ◽

Fred Clubb ◽

Brian E. Applegate ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Fluorescence Lifetime ◽

Biological Tissue ◽

Imaging System ◽

Fluorescence Lifetime Imaging ◽

Optical Coherence ◽

Lifetime Imaging ◽

Biochemical Imaging

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Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization

Optics Letters ◽

10.1364/ol.40.003296 ◽

2015 ◽

Vol 40 (14) ◽

pp. 3296 ◽

Cited By ~ 11

Author(s):

Ximeng Y. Dow ◽

Shane Z. Sullivan ◽

Ryan D. Muir ◽

Garth J. Simpson

Keyword(s):

Fluorescence Lifetime ◽

Imaging System ◽

Fluorescence Lifetime Imaging ◽

Two Photon ◽

Lifetime Imaging

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Construction of a Fluorescence Lifetime Imaging System and its Application to Biological Systems and Polymer Materials

Journal of the Spectroscopical Society of Japan ◽

10.5111/bunkou.55.31 ◽

2006 ◽

Vol 55 (1) ◽

pp. 31-39 ◽

Cited By ~ 5

Author(s):

Takakazu NAKABAYASHI ◽

Toshifumi IIMORI ◽

Masataka KINJO ◽

Nobuhiro OHTA

Keyword(s):

Fluorescence Lifetime ◽

Imaging System ◽

Biological Systems ◽

Polymer Materials ◽

Fluorescence Lifetime Imaging ◽

Lifetime Imaging

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Targetable Conformationally Restricted Cyanines Enable Photon-Count Limited Applications

10.26434/chemrxiv.14720883 ◽

2021 ◽

Author(s):

Patrick Eiring ◽

Ryan McLaughlin ◽

Siddharth Matikonda ◽

HAN ZHONGYING ◽

Lennart Grabenhorst ◽

...

Keyword(s):

Single Molecule ◽

Fluorescence Lifetime ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Ring System ◽

Fluorescence Lifetime Imaging ◽

Conformationally Restricted ◽

Lifetime Imaging ◽

Tertiary Amide ◽

Imaging Performance

Cyanine dyes are exceptionally useful probes for a range of fluorescence-based applications. We recently demonstrated that appending a ring system to the pentamethine cyanine ring system improves the quantum yield and extends the fluorescence lifetime. Here, we report an optimized synthesis of persulfonated variants that enable efficient labeling of nucleic acids and proteins. We demonstrate that a bifunctional sulfonated tertiary amide significantly improves the optical properties of the resulting bioconjugates. These new conformationally restricted cyanines are compared to parent species in a range of contexts including their use on a DNA-nano-antenna, in single-molecule Förster resonance energy transfer (FRET) applications, far-red fluorescence lifetime imaging microscopy (FLIM), and single-molecule localization microscopy. These efforts define contexts in which eliminating cyanine isomerization provides meaningful benefits to imaging performance.

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