Influence of Post-Exercise Carbohydrate-Protein Ingestion on Muscle Glycogen Metabolism in Recovery and Subsequent Running Exercise

2016 ◽  
Vol 26 (6) ◽  
pp. 572-580 ◽  
Author(s):  
Abdullah F. Alghannam ◽  
Dawid Jedrzejewski ◽  
James Bilzon ◽  
Dylan Thompson ◽  
Kostas Tsintzas ◽  
...  
Keyword(s):  
Muscle Glycogen ◽  
Glycogen Metabolism ◽  
Dry Mass ◽  
Treadmill Running ◽  
Time To Exhaustion ◽  
Short Term ◽  
Double Blind ◽  
Protein Ingestion ◽  
Double Blind Design ◽  
Muscle Biopsies

We examined whether carbohydrate-protein ingestion influences muscle glycogen metabolism during short-term recovery from exhaustive treadmill running and subsequent exercise. Six endurance-trained individuals underwent two trials in a randomized double-blind design, each involving an initial run-to-exhaustion at 70% VO2max (Run-1) followed by 4-h recovery (REC) and subsequent run-to-exhaustion at 70% VO2max (Run-2). Carbohydrate-protein (CHO-P; 0.8 g carbohydrate·kg body mass [BM-1]·h-1 plus 0.4 g protein·kg BM-1·h-1) or isocaloric carbohydrate (CHO; 1.2 g carbohydrate·kg BM-1·h-1) beverages were ingested at 30-min intervals during recovery. Muscle biopsies were taken upon cessation of Run-1, postrecovery and fatigue in Run-2. Time-to-exhaustion in Run-1 was similar with CHO and CHO-P (81 ± 17 and 84 ± 19 min, respectively). Muscle glycogen concentrations were similar between treatments after Run-1 (99 ± 3 mmol·kg dry mass [dm-1]). During REC, muscle glycogen concentrations increased to 252 ± 45 mmol·kg dm-1 in CHO and 266 ± 30 mmol·kg dm-1 in CHO-P (p = .44). Muscle glycogen degradation during Run-2 was similar between trials (3.3 ± 1.4 versus 3.5 ± 1.9 mmol·kg dm-1·min-1 in CHO and CHO-P, respectively) and no differences were observed at the respective points of exhaustion (93 ± 21 versus 100 ± 11 mmol·kg dm-1; CHO and CHO-P, respectively). Similarly, time-to-exhaustion was not different between treatments in Run-2 (51 ± 13 and 49 ± 15 min in CHO and CHO-P, respectively). Carbohydrate-protein ingestion equally accelerates muscle glycogen resynthesis during short-term recovery from exhaustive running as when 1.2 g carbohydrate·kg BM-1·h-1 are ingested. The addition of protein did not alter muscle glycogen utilization or time to fatigue during repeated exhaustive running.

Regulation of muscle glycogen phosphorylase activity following short-term endurance training

1996 ◽  
Vol 270 (2) ◽  
pp. E328-E335 ◽  
Author(s):  
A. Chesley ◽  
G. J. Heigenhauser ◽  
L. L. Spriet
Keyword(s):  
Endurance Training ◽  
Muscle Glycogen ◽  
Glycogen Phosphorylase ◽  
Citrate Synthase ◽  
Phosphorylase Activity ◽  
Short Term ◽  
Mitochondrial Potential ◽  
Before And After ◽  
Glycogen Phosphorylase Activity ◽  
Muscle Biopsies

The purpose of this study was to examine the regulation (hormonal, substrate, and allosteric) of muscle glycogen phosphorylase (Phos) activity and glycogenolysis after short-term endurance training. Eight untrained males completed 6 days of cycle exercise (2 h/day) at 65% of maximal O2 uptake (Vo2max). Before and after training subjects cycled for 15 min at 80% of Vo2max, and muscle biopsies and blood samples were obtained at 0 and 30 s, 7.5 and 15 min, and 0, 5, 10, and 15 min of exercise. Vo2max was unchanged with training but citrate synthase (CS) activity increased by 20%. Muscle glycogenolysis was reduced by 42% during the 15-min exercise challenge following training (198.8 +/- 36.9 vs. 115.4 +/- 25.1 mmol/kg dry muscle), and plasma epinephrine was blunted at 15 min of exercise. The Phos a mole fraction was unaffected by training. Muscle phosphocreatine utilization and free Pi and AMP accumulations were reduced with training at 7.5 and 15 min of exercise. It is concluded that posttransformational control of Phos, exerted by reductions in substrate (free Pi) and allosteric modulator (free AMP) contents, is responsible for a blunted muscle glycogenolysis after 6 days of endurance training. The increase in CS activity suggests that the reduction of muscle glycogenolysis was due in part to an enhanced mitochondrial potential.

The effect of magnesium oxide supplementation on muscle glycogen metabolism before and after exercise and at slaughter in sheep

2001 ◽  
Vol 52 (7) ◽  
pp. 723 ◽  
Author(s):  
G. E. Gardner ◽  
R. H. Jacob ◽  
D. W. Pethick
Keyword(s):  
Magnesium Oxide ◽  
Muscle Glycogen ◽  
Glycogen Metabolism ◽  
Post Exercise ◽  
Exercise Regimen ◽  
Before And After ◽  
Series Of Experiments ◽  
Glycogen Repletion ◽  
Exercise Muscle ◽  
Muscle Biopsies

This study was a series of experiments designed to test the influence of supplemental magnesium oxide (MgO) on muscle glycogen concentration in sheep exposed to stress (exercise) and the commercial slaughter process, and to test the effectiveness of this supplement in the commercial scenario. In Expt 1, Merino wethers maintained on a mixed ration (metabolisable energy 11 MJ/kg and crude protein 16.3% in DM) were supplemented with MgO at the rate of 0%, 0.5%, or 1% of their ration for 10 days prior to a single bout of exercise and for 10 days prior to slaughter at a commercial abattoir. The exercise regimen consisted of 4 intervals of 15 min, with muscle biopsies taken by biopsy drill from the m. semimembranosis (SM) and m. semitendinosis (ST) pre-exercise and immediately post-exercise, and at 36 and 72 h post-exercise. Muscle biopsies were also taken 1 week prior to slaughter from the SM and ST, with further samples taken approximately 30 min post-slaughter. Ultimate pH (pHu) of the SM, ST, and m. longissimus dorsi (LD) was measured 48 h after slaughter. Sheep supplemented with MgO lost less muscle glycogen in the ST during exercise, and repleted more muscle glycogen in the SM during the post-exercise repletion phase, than unsupplemented sheep. The supplemented animals also had higher muscle glycogen concentrations in the ST at slaughter. In Expt 2, MgO was administered to Merino wether lambs for 4 days prior to slaughter in the form of a water-borne slurry at a rate equivalent to 1% of their ration. This treatment resulted in significantly reduced muscle glycogen concentrations in both the SM and ST at slaughter. In Expts 3–5, MgO was used as an ‘in-feed’ supplement in the commercial scenario. In each case, slaughter-weight Merino lambs were supplemented with MgO at the rate of 1% of their ration for 4 days prior to commercial slaughter. Positive responses were seen in 2 of the 3 experiments, with increased glycogen concentrations and a reduced pHu. The animals that demonstrated no response to MgO had the lowest pHu after slaughter, suggesting a minimal stress load, thus providing very little scope for an effect of the MgO supplement. We conclude that MgO can reduce the effects of exercise, leading to a subsequent reduction in glycogen loss, and an increase in the rate of glycogen repletion in skeletal muscle following exercise. The results support MgO supplementation as a viable option for reducing the stress associated with commercial slaughter.

Effects of Caffeine Ingestion on Exercise-induced Change during High-Intensity Intermittent Exercise

1995 ◽  
Vol 5 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Isaiah Trice ◽  
Emily M. Haymes
Keyword(s):  
Blood Glucose ◽  
Perceived Exertion ◽  
Intermittent Exercise ◽  
Time To Exhaustion ◽  
Double Blind ◽  
Serum Free Fatty Acid ◽  
Caffeine Ingestion ◽  
Serum Free ◽  
Placebo Trial ◽  
Double Blind Design

In this study a double-blind design was used to determine the effect of caffeine on time to exhaustion and on associated metabolic and circulatory measures. Eight male subjects ingested either caffeine (5 mg/kg body weight) or a placebo 1 hr prior to exercise at 85-90% of maximum workload. Subjects were encouraged to complete three 30-min intermittent cycling periods at 70 rpm with 5 min rest between each. The exercise was terminated when the subject failed to complete three 30-min periods or failed to maintain 70 rpm for at least 15 s consecutively. Serum free fatty acids, glycerol, blood glucose, lactate, perceived exertion, heart rate, andcost were measured. The time to exhaustion was significantly longer during the caffeine trial than during the placebo trial. Serum free fatty acid levels were significantly different between trials. The decline in blood glucose levels was significantly less during the caffeine trial than during the placebo trial. There were no significant differences between trials for the other measures. It was concluded that caffeine increases time to exhaustion when trained subjects cycled intermittently at high levels of intensity.

Modulation of autophagy signaling with resistance exercise and protein ingestion following short-term energy deficit

2015 ◽  
Vol 309 (5) ◽  
pp. R603-R612 ◽  
Author(s):  
William J. Smiles ◽  
José L. Areta ◽  
Vernon G. Coffey ◽  
Stuart M. Phillips ◽  
Daniel R. Moore ◽  
...  
Keyword(s):  
Resistance Exercise ◽  
Contractile Activity ◽  
Fat Free Mass ◽  
Energy Deficit ◽  
Short Term ◽  
Protein Levels ◽  
Protein Ingestion ◽  
Term Energy ◽  
Muscle Biopsies ◽  
Time Point

Autophagy contributes to remodeling of skeletal muscle and is sensitive to contractile activity and prevailing energy availability. We investigated changes in targeted genes and proteins with roles in autophagy following 5 days of energy balance (EB), energy deficit (ED), and resistance exercise (REX) after ED. Muscle biopsies from 15 subjects (8 males, 7 females) were taken at rest following 5 days of EB [45 kcal·kg fat free mass (FFM)−1·day−1] and 5 days of ED (30 kcal·kg FFM−1·day−1). After ED, subjects completed a bout of REX and consumed either placebo (PLA) or 30 g whey protein (PRO) immediately postexercise. Muscle biopsies were obtained at 1 and 4 h into recovery in each trial. Resting protein levels of autophagy-related gene protein 5 (Atg5) decreased after ED compared with EB (∼23%, P < 0.001) and remained below EB from 1 to 4 h postexercise in PLA (∼17%) and at 1 h in PRO (∼18%, P < 0.05). In addition, conjugated Atg5 (cAtg12) decreased below EB in PLA at 4 h (∼20, P < 0.05); however, its values were increased above this time point in PRO at 4 h alongside increases in FOXO1 above EB (∼22–26%, P < 0.05). Notably, these changes were subsequent to increases in unc-51-like kinase 1Ser757 phosphorylation (∼60%) 1 h postexercise in PRO. No significant changes in gene expression of selected autophagy markers were found, but EGR-1 increased above ED and EB in PLA (∼417–864%) and PRO (∼1,417–2,731%) trials 1 h postexercise ( P < 0.001). Postexercise protein availability, compared with placebo, can selectively promote autophagic responses to REX in ED.

No evidence of oxidant stress during high-intensity rowing training

1993 ◽  
Vol 74 (5) ◽  
pp. 2140-2145 ◽  
Author(s):  
A. R. Dernbach ◽  
W. M. Sherman ◽  
J. C. Simonsen ◽  
K. M. Flowers ◽  
D. R. Lamb
Keyword(s):  
Muscle Glycogen ◽  
High Intensity ◽  
Tissue Sample ◽  
Oxidant Stress ◽  
Thiobarbituric Acid ◽  
Strenuous Exercise ◽  
Double Blind ◽  
Training Time ◽  
Stress Markers ◽  
Double Blind Design

In untrained subjects, strenuous exercise provokes the appearance of oxidant stress markers in blood and muscle. On the other hand, trained muscle is resistant to oxidant stress unless exercise challenges the muscle glycogen supply. It is not known whether chronic high-intensity exercise alters the susceptibility of skeletal muscle to oxidant stress, whether there are gender-related differences in markers of oxidant stress, or whether elevating muscle glycogen stores by increasing dietary carbohydrate can minimize any exercise-related oxidant stress. To address these issues, collegiate rowers (12 men, 11 women) were randomly assigned to a moderate-(MOD, 5 g/kg body wt) or high-carbohydrate (HI, 10 g/kg) diet in a double-blind design and underwent strenuous training for 4 wk. Training in the A.M. was 40 min at 70% maximal O2 consumption (VO2); in the P.M. it was either three 2,500-m time trials (to assess power output) or aerobic and lactate tolerance training. Total daily training time was 65 min at 70% maximal VO2 and 38 min at > or = 90% maximal VO2. Thrice-weekly morning blood samples were assayed for serum creatine kinase (CK), plasma thiobarbituric acid-reactive substances (TBARS), and serum beta-glucuronidase (beta-Gluc). Weekly muscle biopsies were obtained for analysis of glycogen and, when tissue sample quantity allowed, TBARS. HI rowers produced more power and improved power more (10.7 +/- 1.0 vs. 1.6 +/- 1.6%) over the 4 wk than did the MOD rowers. Preexercise muscle glycogen concentration was maintained at 119 mmol/kg in MOD but increased 65% in HI rowers (P < 0.05).

Effects of Caffeine Ingestion on Exercise Testing: A Meta-Analysis

2004 ◽  
Vol 14 (6) ◽  
pp. 626-646 ◽  
Author(s):  
Mike Doherty ◽  
Paul M. Smith
Keyword(s):  
Exercise Testing ◽  
Meta Analysis ◽  
Time To Exhaustion ◽  
Inclusion Criteria ◽  
Short Term ◽  
Double Blind ◽  
Caffeine Ingestion ◽  
Ergogenic Effects ◽  
Endurance Testing ◽  
Type Of Exercise

This study used the meta-analytic approach to examine the effects of caffeine ingestion on exercise testing. Forty double-blind studies with 76 effect sizes (ES) met the inclusion criteria. The type of exercise test was classified as endurance, graded, or short-term. In comparison with placebo, caffeine improved test outcome by 12.3% (95% CI, 9.1 to 15.4), which was equivalent to an overall ES of 0.41 (95% CI, 0.31 to 0.51). Endurance exercise significantly improved test outcome (P < 0.05) more than either graded or short-term exercise. When exercise protocol was examined, time-to-exhaustion (Tlim) protocols had a significantly greater (P < 0.05) ES than either the graded or the non-Tlim protocol(s). The results from this meta-analysis confirm the ergogenic effects of caffeine, particularly for endurance testing that use Tlim protocols.

Effect of induced erythrocythemia on aerobic work capacity

1980 ◽  
Vol 48 (4) ◽  
pp. 636-642 ◽  
Author(s):  
F. J. Buick ◽  
N. Gledhill ◽  
A. B. Froese ◽  
L. Spriet ◽  
E. C. Meyers
Keyword(s):  
Aerobic Capacity ◽  
Work Capacity ◽  
Time To Exhaustion ◽  
O2 Consumption ◽  
Maximal Aerobic Capacity ◽  
Double Blind ◽  
Double Blind Design ◽  
Trained Runners ◽  
Aerobic Work Capacity ◽  
Distinct Increase

The aerobic work capacity of 11 highly trained runners was studied employing a double-blind design 1) before phlebotomy (C1), 2) following restoration of normocythemia (C2), 3) after a sham reinfusion of 50 ml of saline (sham), 4) following autologous reinfusion of approximately 900 ml of freeze-preserved blood (reinfusion), and 5) upon reestablishment of control hematologic levels after erythrocythemia (C3). There were no hematologic differences among C1, C2, sham, and C3, but following reinfusion, hemoglobin was significantly elevated (15.7-16.7 g . 100 ml-1). Maximum O2 consumption (VO2max) and running time to exhaustion were significantly increased 24 h postreinfusion (5.11-5.37 l . min-1 and 7.20-9.65 min, respectively) and 7 days postreinfusion. When sham preceded reinfusion, VO2 max and time to exhaustion were the same as control. However, 16 wk postreinfusion, despite the return to normal hematologic values, VO2max remained significantly above control levels at sham and C3. These findings indicate that there is a distinct increase in VO2max following induced erythrocythemia and suggest that oxygen transport limits maximal aerobic capacity.

Hepatic and skeletal muscle glycogen metabolism in rats with short-term cholestasis

2002 ◽  
Vol 36 (1) ◽  
pp. 22-29 ◽  
Author(s):  
Corinne Lang ◽  
Markus Schäfer ◽  
Laszlo Varga ◽  
Arthur Zimmermann ◽  
Stephan Krähenbühl ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Glycogen ◽  
Glycogen Metabolism ◽  
Short Term ◽  
Skeletal Muscle Glycogen

The Effect of 4 Weeks Treatment with a 2-gram Daily Dose of Pyruvate on Body Composition in Healthy Trained Men

2009 ◽  
Vol 79 (3) ◽  
pp. 173-179 ◽  
Author(s):  
Sergej M. Ostojic ◽  
Zlatko Ahmetovic
Keyword(s):  
Body Composition ◽  
Muscle Mass ◽  
Body Mass ◽  
Placebo Treatment ◽  
The Body ◽  
Short Term ◽  
Double Blind ◽  
Daily Dose ◽  
Double Blind Design ◽  
Regional Muscle

The principal aim of this study was to determine the effects of short-term (28 days) pyruvate supplementation on body composition in young healthy men. Twenty-two young (mean age, 22.2 ± 2.7 years) male soccer players were allocated to two randomly assigned groups in a placebo-controlled, double-blind design. Subjects in the pyruvate group orally ingested tablets that contained pyruvate at a dose of 2 g per day in two equal doses for 4 weeks. There were no statistically significant changes in weight and body-mass index (BMI) within or between groups (p>0.05) after the supplementation protocol. Percentage of body fat decreased similarly in both pyruvate and placebo groups after the supplementation protocol (p>0.05). There were no changes in waist-to-hip ratio (WHR), arm fat index (AFI), and total or regional muscle mass within or between groups (p>0.05). No subject reported any side effects from pyruvate or placebo treatment. The results of the present study indicate that supplementation with pyruvate during training does not significantly alter the body mass, fat, and muscle mass in healthy trained men. Pyruvate supplementation appears to be ineffective as a fat loss strategy in young athletes.

Acute ingestion of hydrogen-rich water does not improve incremental treadmill running performance in endurance-trained athletes

2020 ◽  
Vol 45 (5) ◽  
pp. 513-519 ◽  
Author(s):  
Cheong Hwa Ooi ◽  
Siew Kit Ng ◽  
Eshaifol Azam Omar
Keyword(s):  
Oxygen Uptake ◽  
Maximal Oxygen Uptake ◽  
Perceived Exertion ◽  
Controlled Trial ◽  
Physiological Responses ◽  
Endurance Performance ◽  
Treadmill Running ◽  
Time To Exhaustion ◽  
Double Blind ◽  
Acute Ingestion

There is emerging evidence that hydrogen-rich water (H2-water) has beneficial effects on the physiological responses to exercise. However, few studies investigate its ergogenic potential. This randomized controlled trial examined the effects of H2-water ingestion on physiological responses and exercise performance during incremental treadmill running. In a double-blind crossover design, 14 endurance-trained male runners (age, 34 ± 4 years; body mass, 63.1 ± 7.2 kg; height, 1.72 ± 0.05 m) were randomly assigned to ingest 2 doses of 290-mL H2-water or placebo on each occasion. The first bolus was given before six 4-min submaximal running bouts, and the second bolus was consumed before the maximal incremental running test. Expired gas, heart rate (HR), and ratings of perceived exertion (RPE) were recorded; blood samples were collected at the end of each submaximal stage and post maximal running test. Cardiorespiratory responses, RPE, and blood gas indices were not significantly different at each submaximal running intensity (range: 34%–91% maximal oxygen uptake) between H2-water and placebo trials. No statistical difference was observed in running time to exhaustion (618 ± 126 vs. 619 ± 113 s), maximal oxygen uptake (56.9 ± 4.4 vs. 57.1 ± 4.7 mL·kg−1·min−1), maximal HR (184 ± 7 vs. 184 ± 7 beat·min−1), and RPE (19 ± 1 vs. 19 ± 1) in the runners between the trials. The results suggest that the ingestion of 290 mL of H2-water before submaximal treadmill running and an additional dose before the subsequent incremental running to exhaustion were not sufficiently ergogenic in endurance-trained athletes. Novelty Acute ingestion of H2-water does not seem to be ergogenic for endurance performance. A small dose of H2-water does not modulate buffering capacity during intense endurance exercise in athletes.

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