Epidermal Growth Factor Receptor-Dependent and -Independent Pathways in Hydrogen Peroxide-Induced Mitogen-Activated Protein Kinase Activation in Cardiomyocytes and Heart Fibroblasts

2004 ◽  
Vol 312 (3) ◽  
pp. 1179-1186 ◽  
Author(s):  
Sally Purdom ◽  
Qin M. Chen
Keyword(s):  
Hydrogen Peroxide ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Protein Kinase ◽  
Growth Factor Receptor ◽  
Mitogen Activated Protein Kinase ◽  
Kinase Activation ◽  
Protein Kinase Activation ◽  
Mitogen Activated Protein ◽  
Epidermal Growth

scholarly journals Peptide YY Y1 receptor activates mitogen-activated protein kinase and proliferation in gut epithelial cells via the epidermal growth factor receptor

2000 ◽  
Vol 350 (3) ◽  
pp. 655-661 ◽  
Author(s):  
Peter J. MANNON ◽  
Jennifer M. MELE
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Protein Kinase ◽  
Epithelial Cells ◽  
Peptide Yy ◽  
Growth Factor Receptor ◽  
Mitogen Activated Protein Kinase ◽  
Y1 Receptor ◽  
Mitogen Activated Protein ◽  
Epidermal Growth

The G-protein-coupled peptide YY (PYY)/neuropeptide Y Y1 receptor (Y1R) subtype is highly expressed in the proliferative zone of human colonic crypt epithelial cells but biochemical and biological support for growth effects have been lacking. Using a model gut epithelial cell system, we have stably expressed the human Y1R in IEC-6 cells and show that the Y1R does couple to mitogen-activated protein kinase (MAPK) phosphorylation and cell growth. This pathway uses pertussis-toxin-sensitive G-proteins and βγ subunits, inhibited by co-transfected α-transducin. The Src-family tyrosine kinase inhibitor PP1, as well as specific inhibition of the epidermal growth factor receptor tyrosine kinase (EGFR TK) by PD153035, also blocks PYY stimulation of MAPK. This pathway further requires protein kinase C with EGFR TK inhibition blocking PYY-induced protein kinase Cε (PKCε) translocation to the cell membrane. Finally, we show that PYY stimulates growth in Y1R-expressing gut epithelial cells that is dependent on EGFR TK activity. These results demonstrate a novel pathway involving Gi/Go protein, EGFR and PKC to activate MAPK. Further, they support a role for PYY and the Y1R in regulating growth in human colonic epithelium.

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