In Vivo α1-Adrenergic Lipolytic Activity in Subcutaneous Adipose Tissue of Obese Subjects

Obesity is associated with elevated inflammatory signals from various adipose tissue depots. This study aimed to evaluate release of regulated on activation, normal T cell expressed and secreted (RANTES) by human adipose tissue in vivo and ex vivo, in reference to monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6) release. Arteriovenous differences of RANTES, MCP-1, and IL-6 were studied in vivo across the abdominal subcutaneous adipose tissue in healthy Caucasian subjects with a wide range of adiposity. Systemic levels and ex vivo RANTES release were studied in abdominal subcutaneous, gastric fat pad, and omental adipose tissue from morbidly obese bariatric surgery patients and in thoracic subcutaneous and epicardial adipose tissue from cardiac surgery patients without coronary artery disease. Arteriovenous studies confirmed in vivo RANTES and IL-6 release in adipose tissue of lean and obese subjects and release of MCP-1 in obesity. However, in vivo release of MCP-1 and RANTES, but not IL-6, was lower than circulating levels. Ex vivo release of RANTES was greater from the gastric fat pad compared with omental ( P = 0.01) and subcutaneous ( P = 0.001) tissue. Epicardial adipose tissue released less RANTES than thoracic subcutaneous adipose tissue in lean ( P = 0.04) but not obese subjects. Indexes of obesity correlated with epicardial RANTES but not with systemic RANTES or its release from other depots. In conclusion, RANTES is released by human subcutaneous adipose tissue in vivo and in varying amounts by other depots ex vivo. While it appears unlikely that the adipose organ contributes significantly to circulating levels, local implications of this chemokine deserve further investigation.

Download Full-text

In Vivo Increase in -Adrenergic Lipolytic Response in Subcutaneous Adipose Tissue of Obese Subjects Submitted to a Hypocaloric Diet

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.82.1.63 ◽

1997 ◽

Vol 82 (1) ◽

pp. 63-69 ◽

Cited By ~ 8

Author(s):

P. Barbe

Keyword(s):

Adipose Tissue ◽

Subcutaneous Adipose Tissue ◽

Hypocaloric Diet ◽

Obese Subjects ◽

Subcutaneous Adipose

Download Full-text

In vivo human lipolytic activity in preperitoneal and subdivisions of subcutaneous abdominal adipose tissue

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.2001.281.5.e1110 ◽

2001 ◽

Vol 281 (5) ◽

pp. E1110-E1114 ◽

Cited By ~ 34

Author(s):

Lotte H. Enevoldsen ◽

Lene Simonsen ◽

Bente Stallknecht ◽

Henrik Galbo ◽

Jens Bülow

Keyword(s):

Adipose Tissue ◽

Abdominal Wall ◽

Subcutaneous Adipose Tissue ◽

Lipolytic Activity ◽

Normal Weight ◽

Round Ligament ◽

Epinephrine Infusion ◽

Adipose Tissues ◽

Subcutaneous Adipose

We studied eight normal-weight male subjects to examine whether the lipolytic rate of deep subcutaneous and preperitoneal adipose tissues differs from that of superficial abdominal subcutaneous adipose tissue. The lipolytic rates in the superficial anterior and deep posterior subcutaneous abdominal adipose tissues and in the preperitoneal adipose tissue in the round ligament were measured by microdialysis and 133Xe washout under basal, postabsorptive conditions and during intravenous epinephrine infusion (0.15 nmol · kg−1 · min−1). Both in the basal state and during epinephrine stimulation, the superficial subcutaneous adipose tissue had higher interstitial glycerol concentrations than the two other depots. Similarly, the calculated glycerol outputs from the superficial depot were significantly higher than those from the deep subcutaneous and the preperitoneal depots. Thus, it is concluded that the lipolytic rate of the superficial subcutaneous adipose tissue on the anterior abdominal wall is higher than that of the deep subcutaneous adipose tissue on the posterior abdominal wall and that of the preperitoneal adipose tissue in the round ligament.

Download Full-text