scholarly journals In situ repair abilities of human umbilical cord–derived mesenchymal stem cells and autocrosslinked hyaluronic acid gel complex in rhesus monkeys with intrauterine adhesion

2020 ◽  
Vol 6 (21) ◽  
pp. eaba6357
Author(s):  
Lingjuan Wang ◽  
Chuanhu Yu ◽  
Tianli Chang ◽  
Mengdi Zhang ◽  
Su Song ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Umbilical Cord ◽  
Human Life ◽  
Human Umbilical Cord ◽  
Intrauterine Adhesion ◽  
Acid Gel ◽  
Hyaluronic Acid Gel

Increasing occurrence of moderate to severe intrauterine adhesion (IUA) is seriously affecting the quality of human life. The aim of the study was to establish IUA models in nonhuman primates and to explore the dual repair effects of human umbilical cord–derived mesenchymal stem cells (huMSCs) loaded on autocrosslinked hyaluronic acid gel (HA-GEL) on endometrial damage and adhesion. Here, we recorded the menstrual cycle data in detail with uterine cavities observed and endometrial tissues detected after intervention, and the thicker endometria, decreased amount of fibrotic formation, increased number of endometrium glands, etc., suggested that both HA-GEL and huMSC/HA-GEL complexes could partially repair IUA caused by mechanical injury, but huMSC/HA-GEL complex transplantation had notable dual repair effects: a reliable antiadhesion property and the promotion of endometrial regeneration.

scholarly journals The assessment of CD146-based cell sorting and telomere length analysis for establishing the identity of mesenchymal stem cells in human umbilical cord

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 126 ◽  
Author(s):  
Dimitrios Kouroupis ◽  
Sarah M. Churchman ◽  
Dennis McGonagle ◽  
Elena A. Jones
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Cell Sorting ◽  
Adult Stem Cells ◽  
Human Umbilical Cord ◽  
Length Analysis

Adult stem cells are characterised by longer telomeres compared to mature cells from the same tissue. In this study, candidate CD146+ umbilical cord (UC) mesenchymal stem cells (MSCs) were purified by cell sorting from UC tissue digests and their telomere lengths were measured in comparison to donor-matched CD146-negative fraction.UC tissue fragments were enzymatically treated with collagenase and the cells were used for cell sorting, colony-forming fibroblast (CFU-F) assay or for long-term MSC cultivation. Telomere lengths were measured by qPCR in both culture-expanded MSCs and candidate native UC MSCs. Immunohistochemistry was undertaken to study the topography of CD146+ cells.Culture-expanded UC MSCs had a stable expression of CD73, CD90 and CD105, whereas CD146 declined in later passages which correlated with the shortening of telomeres in the same cultures. In three out of four donors, telomeres in candidate native UC MSCs (CD45-CD235α-CD31-CD146+) were longer compared to donor-matched CD146- population (CD45-CD235α-CD31-CD146-). The frequency of CD45-CD235α-CD31-CD146+ cells measured by flow cytometry was ~1000-fold above that of donor-matched CFU-Fs (means 10.4% and 0.01%, respectively). CD146+ cells were also abundant in situ having a broad topography including high levels of positivity in muscle areas in addition to vessels.

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