Minimally invasive electroceutical catheter for endoluminal defect sealing

Surgical repair of lumen defects is associated with periprocedural morbidity and mortality. Endovascular repair with tissue adhesives may reduce host tissue damage, but current bioadhesive designs do not support minimally invasive deployment. Voltage-activated tissue adhesives offer a new strategy for endoluminal repair. To facilitate the clinical translation of voltage-activated adhesives, an electroceutical patch (ePATCH) paired with a minimally invasive catheter with retractable electrodes (CATRE) is challenged against the repair of in vivo and ex vivo lumen defects. The ePATCH/CATRE platform demonstrates the sealing of lumen defects up to 2 millimeters in diameter on wet tissue substrates. Water-tight seals are flexible and resilient, withstanding over 20,000 physiological relevant stress/strain cycles. No disruption to electrical signals was observed when the ePATCH was electrically activated on the beating heart. The ePATCH/CATRE platform has diverse potential applications ranging from endovascular treatment of pseudo-aneurysms/fistulas to bioelectrodes toward electrophysiological mapping.

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Barnacle-Inspired Paste for Instant Hemostatic Tissue Sealing

10.1101/2020.12.12.422505 ◽

2020 ◽

Author(s):

Hyunwoo Yuk ◽

Jingjing Wu ◽

Xinyu Mao ◽

Claudia E. Varela ◽

Ellen T. Roche ◽

...

Keyword(s):

Ex Vivo ◽

Tissue Adhesives ◽

Interfacial Toughness ◽

Strong Shear ◽

Potential Applications ◽

Gentle Pressure ◽

Adhesive Proteins ◽

Tissue Sealing

Whilst sealing damaged tissues by adhesives has potential advantages over suturing or stapling, existing tissue adhesives cannot form rapid or robust adhesion on tissues covered with body fluids such as blood. In contrast, the glues of barnacles, consisting of a lipid-rich matrix and adhesive proteins, and can strongly adhere to wet and contaminated surfaces. Here we report a barnacle-inspired paste capable of forming instant robust hemostatic sealing of diverse tissues. The paste is composed of a hydrophobic oil matrix and bioadhesive microparticles to implement the barnacle-inspired mechanism to repel blood through the hydrophobic matrix. Subsequently, the bioadhesive microparticles crosslink with underlying tissues under gentle pressure. The barnacle-inspired paste can provide tough (interfacial toughness over 300 J m-2) and strong (shear and tensile strength over 70 kPa, burst pressure over 350 mmHg) hemostatic sealing of a broad range of tissues within five seconds. We validate in vitro and in vivo biocompatibility and biodegradability of the barnacle-inspired paste in rodent models. We further demonstrate potential applications of the barnacle-inspired paste for instant hemostatic sealing in ex vivo porcine aorta, in vivo rat liver and heart models.

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Microfluidics in male reproduction: is ex vivo culture of primate testis tissue a future strategy for ART or toxicology research?

MHR Basic science of reproductive medicine ◽

10.1093/molehr/gaaa006 ◽

2020 ◽

Vol 26 (3) ◽

pp. 179-192 ◽

Cited By ~ 1

Author(s):

Swati Sharma ◽

Bastien Venzac ◽

Thomas Burgers ◽

Séverine Le Gac ◽

Stefan Schlatt

Keyword(s):

Male Infertility ◽

Functional Organization ◽

Ex Vivo ◽

Significant Rise ◽

Male Reproduction ◽

Potential Applications ◽

On Chip ◽

Bio Engineering

Abstract The significant rise in male infertility disorders over the years has led to extensive research efforts to recapitulate the process of male gametogenesis in vitro and to identify essential mechanisms involved in spermatogenesis, notably for clinical applications. A promising technology to bridge this research gap is organ-on-chip (OoC) technology, which has gradually transformed the research landscape in ART and offers new opportunities to develop advanced in vitro culture systems. With exquisite control on a cell or tissue microenvironment, customized organ-specific structures can be fabricated in in vitro OoC platforms, which can also simulate the effect of in vivo vascularization. Dynamic cultures using microfluidic devices enable us to create stimulatory effect and non-stimulatory culture conditions. Noteworthy is that recent studies demonstrated the potential of continuous perfusion in OoC systems using ex vivo mouse testis tissues. Here we review the existing literature and potential applications of such OoC systems for male reproduction in combination with novel bio-engineering and analytical tools. We first introduce OoC technology and highlight the opportunities offered in reproductive biology in general. In the subsequent section, we discuss the complex structural and functional organization of the testis and the role of the vasculature-associated testicular niche and fluid dynamics in modulating testis function. Next, we review significant technological breakthroughs in achieving in vitro spermatogenesis in various species and discuss the evidence from microfluidics-based testes culture studies in mouse. Lastly, we discuss a roadmap for the potential applications of the proposed testis-on-chip culture system in the field of primate male infertility, ART and reproductive toxicology.

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A minimally invasive catheter-based ultrasound technology for therapeutic interventions in brain: initial preclinical studies

Neurosurgical FOCUS ◽

10.3171/2017.11.focus17631 ◽

2018 ◽

Vol 44 (2) ◽

pp. E13 ◽

Cited By ~ 4

Author(s):

Goutam Ghoshal ◽

Lucy Gee ◽

Tamas Heffter ◽

Emery Williams ◽

Corinne Bromfield ◽

...

Keyword(s):

Parkinson’S Disease ◽

Parkinson's Disease ◽

Minimally Invasive ◽

Focused Ultrasound ◽

Ex Vivo ◽

Experimental Studies ◽

Rodent Model ◽

Therapeutic Interventions ◽

Porcine Liver

OBJECTIVEMinimally invasive procedures may allow surgeons to avoid conventional open surgical procedures for certain neurological disorders. This paper describes the iterative process for development of a catheter-based ultrasound thermal therapy applicator.METHODSUsing an ultrasound applicator with an array of longitudinally stacked and angularly sectored tubular transducers within a catheter, the authors conducted experimental studies in porcine liver, in vivo and ex vivo, in order to characterize the device performance and lesion patterns. In addition, they applied the technique in a rodent model of Parkinson’s disease to investigate the feasibility of its application in brain.RESULTSThermal lesions with multiple shapes and sizes were readily achieved in porcine liver. The feasibility of catheter-based focused ultrasound in the treatment of brain conditions was demonstrated in a rodent model of Parkinson’s disease.CONCLUSIONSThe authors show proof of principle of a catheter-based ultrasound system that can create lesions with concurrent thermode-based measurements.

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Individualised Timing of Radio-Guided Parathyroidectomy Using Multi-Phase SPECT/CT Increases In Vivo Sensitivity and Accuracy and Reduces Operating Time: A Randomised Clinical Trial

Diagnostics ◽

10.3390/diagnostics11040677 ◽

2021 ◽

Vol 11 (4) ◽

pp. 677

Author(s):

Martin Formánek ◽

Vladimír Dedek ◽

Michal Koláček ◽

Martin Havel ◽

Karol Zeleník ◽

...

Keyword(s):

Minimally Invasive ◽

Ex Vivo ◽

Operating Time ◽

Randomised Clinical Trial ◽

Minimally Invasive Parathyroidectomy ◽

Group I ◽

Group Ii ◽

99Mtc Mibi ◽

Multi Phase

Background: Minimally invasive parathyroidectomy is the preferred treatment for primary hyperparathyroidism. Despite relatively accurate preoperative information, minimally invasive parathyroidectomy can be challenging, especially in the case of small and ectopic adenomas. Radio guidance aids in both in vivo identification and ex vivo confirmation of adenoma. In vivo accuracy is currently not satisfactory. The present study evaluated whether a beneficial effect (increased sensitivity, specificity, accuracy) is obtained with individualised timing of minimally invasive radio-guided parathyroidectomy (MIRGP) using preoperative multi-phase 99mTc-MIBI single photon emission computed tomography (SPECT)/computed tomography (CT). Methods: This randomised clinical trial was conducted from May 2016 to January 2020 in a tertiary referral hospital. Adult patients with primary hyperparathyroidism sent for 99mTc-MIBI SPECT/CT were included consecutively and randomly assigned to conventional (dual-phase) SPECT/CT and conventional MIRGP (group I) or multi-phase SPECT/CT and individualised MIRGP (group II). One hundred of 106 eligible patients were included, and 83 patients underwent complete intervention. Results: A total of 47 patients in group I and 35 patients in group II were analysed. Group II had a shorter operating time (p = 0.003). The in vivo sensitivity and accuracy of radio guidance was 85.1% in group I and 100% in group II (p = 0.046), and 90.4% in group I and 100% in group II (p = 0.021), respectively. We found no difference in the in vivo specificity and ex vivo parameters between groups. Conclusion: Individualised timing increased the in vivo sensitivity and accuracy of radio guidance and reduced operating time, as some parathyroid adenomas rapidly wash out the radionuclide.

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A mussel-inspired film for adhesion of wet buccal tissue and efficient buccal drug delivery

10.21203/rs.3.rs-69144/v1 ◽

2020 ◽

Author(s):

Shanshan Hu ◽

Xibo Pei ◽

Lunliang Duan ◽

Zhou Zhu ◽

Yanhua Liu ◽

...

Keyword(s):

Erosion Rate ◽

Ex Vivo ◽

Covalent Bonding ◽

Buccal Delivery ◽

Tissue Adhesives ◽

Drug Bioavailability ◽

Buccal Drug Delivery ◽

Strong Adhesion ◽

Developing System

Abstract Administration of drug via the buccal route has attracted much attention in recent years. However, developing system with satisfactory adhesion in wet conditions and drug bioavailability still remains a challenge. Here, we propose a mussel inspired mucoadhesive film. Ex vivo porcine and in vivo rat models show that the film can achieve strong adhesion with wet buccal tissues. We also demonstrate that the film exhibits tunable mucoadhesion strength and erosion rate. The adhesion mechanism of this film relies on both physical association and covalent bonding between the film and mucus. Then, polydopamine (PDA) modified nanoparticles (NPs) are incorporated into the film and the PDA NPs loaded films show superior advantages to transport across multiple barriers of buccal mucosa with improved drug bioavailability and therapeutic efficacy in oral mucositis models. We anticipate that this platform might aid the development of tissue adhesives and inspire the design of nanoparticle-based buccal delivery systems.

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Inside the beating heart: an in vivo feasibility study on fusing pre- and intra-operative imaging for minimally invasive therapy

International Journal of Computer Assisted Radiology and Surgery ◽

10.1007/s11548-008-0278-6 ◽

2008 ◽

Vol 4 (2) ◽

pp. 113-123 ◽

Cited By ~ 26

Author(s):

Cristian A. Linte ◽

John Moore ◽

Chris Wedlake ◽

Daniel Bainbridge ◽

Gérard M. Guiraudon ◽

...

Keyword(s):

Minimally Invasive ◽

Feasibility Study ◽

Beating Heart ◽

Minimally Invasive Therapy ◽

Invasive Therapy

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Off-pump tricuspid valve repair by automated sutured tricuspid annular plication via transatrial cannulation: preclinical ex vivo and in vivo results

Interactive CardioVascular and Thoracic Surgery ◽

10.1093/icvts/ivz297 ◽

2019 ◽

Vol 30 (4) ◽

pp. 636-645

Author(s):

Paul Werner ◽

Marco Russo ◽

Jude Sauer ◽

Robert Zilberszac ◽

Claus Rath ◽

...

Keyword(s):

Minimally Invasive ◽

Tricuspid Valve ◽

Human Heart ◽

Ex Vivo ◽

Invasive Technique ◽

Tricuspid Valve Repair ◽

Off Pump ◽

Ex Vivo Perfusion ◽

Perfusion Model

Abstract OBJECTIVES Surgical repair is considered the gold standard treatment for severe symptomatic tricuspid valve (TV) regurgitation. However, patients undergoing isolated surgical tricuspid repair face a high perioperative mortality while long-term data on interventional treatment options are currently missing. We investigated a novel, minimally invasive approach for transatrial off-pump beating-heart tricuspid annular plication based on the surgical Hetzer repair. METHODS TV annular plication for the creation of a double-orifice valve using novel devices for automated annular suturing was performed in 10 human heart specimens in an ex vivo perfusion model under endoscopic guidance. Additionally, the technique was tested in an in vivo porcine model using the transatrial access under echocardiographic and fluoroscopic guidance. RESULTS Endoscopically guided conduction of the procedure was successful in all 10 human heart specimens in the ex vivo perfusion model with 1 observed suture pull-through of 60 sutures placed (1.7%). TV measurements yielded significant reductions of the TV septal–lateral diameter (50.9 ± 7.3 vs 42.6 ± 7.9 mm; P = 0.015) and the TV area (1208 ± 399 vs 193 ± 122 mm2; P < 0.0001). TV plication without direct vision using device-embedded intracardiac echocardiography, epicardial echocardiography and fluoroscopy was feasible in both acute animals with no observed device-related adverse events. CONCLUSIONS Successful plication was completed in 10 ex vivo human hearts, additionally proof-of-concept was conducted in 2 animals. We herein present encouraging early preclinical results of a novel minimally invasive technique for TV repair, which warrants further investigation.

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Cyclosporin A-loaded poly(d,l-lactide) nanoparticles: a promising tool for treating alopecia

Nanomedicine ◽

10.2217/nnm-2020-0089 ◽

2020 ◽

Vol 15 (15) ◽

pp. 1459-1469

Author(s):

Bruno Fernandes ◽

Teresa Matamá ◽

Andreia C. Gomes ◽

Artur Cavaco-Paulo

Keyword(s):

Cyclosporin A ◽

Ex Vivo ◽

Skin Permeation ◽

Hair Follicles ◽

Porcine Skin ◽

Skin Cell ◽

Promising Tool ◽

Physicochemical Stability ◽

New Strategy

Background: Alopecia treatments are scarce and lack efficacy. Cyclosporin A (CsA) has hair growth-inducing properties but its poor cutaneous absorption undermines its use in topical treatments. Aim: Development of a new potential topical treatment of alopecia with CsA. Materials & methods: CsA-loaded poly(d,l-lactide) (PLA) nanoparticles were obtained and characterized. Skin permeation was evaluated in ex vivo porcine skin. Results: Nanoparticles with good physicochemical stability increased CsA skin permeation/hair follicles accumulation, compared with a noncolloidal formulation. CsA biocompatibility in NCTC2455 keratinocytes (reference skin cell line) was clearly improved when encapsulated in PLA nanoparticles. Conclusion: This work fosters further in vivo investigation of CsA-loaded PLA nanoparticles as a promising new strategy to treat alopecia, a very traumatic, possibly autoimmune, disease.

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Formulation buoyancy of nanoencapsulated gliclazide using primary, conjugated and deconjugated bile acids

Therapeutic Delivery ◽

10.4155/tde-2019-0058 ◽

2019 ◽

Vol 10 (9) ◽

pp. 573-583

Author(s):

Sangeetha Mathavan ◽

Corina M Ionescu ◽

Bozica Kovacevic ◽

Momir Mikov ◽

Svetlana Golocorbin-Kon ◽

...

Keyword(s):

Bile Acids ◽

Sodium Alginate ◽

Ex Vivo ◽

Permeation Enhancers ◽

Human Bile ◽

Potential Applications ◽

Specific Uptake ◽

The Stability

Aim: Recent studies suggest potential applications of endogenously produced human bile acids as formulation-excipient and drug tissue permeation enhancers in Type 1 diabetes. We aimed to examine the stability, tissue permeation and ex vivo muscle-cell effects of microencapsulated gliclazide (G) incorporated with a primary (chenodeoxycholic acid [CDCA]), a secondary (ursodeoxycholic acid [UDCA]) or a tertiary (taurocholic acid [TCA]) bile acid. Materials & methods: Four formulations made of sodium alginate, CDCA, UDCA and TCA were examined for buoyancy, tissue-enhancing effects ( in vivo) and local ( ex vivo) viability effects. Results & conclusion: CDCA, UDCA and TCA improved buoyancy and cell viability but not tissue-specific uptake. G-TCA-sodium alginate microcapsules exerted hypoglycemic effects, suggesting significant improvement of G gut-uptake by TCA, possibly via improving buoyancy.

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Minimally Invasive Precise Application of Bioadhesives to Prevent IPPROM on a Pregnant Sheep Model

Fetal Diagnosis and Therapy ◽

10.1159/000519910 ◽

2021 ◽

pp. 1-9

Author(s):

Yannick R. Devaud ◽

Senta Stäuble ◽

Ueli Moehrlen ◽

Miriam Weisskopf ◽

Ladina Vonzun ◽

...

Keyword(s):

Ex Vivo ◽

Negative Control ◽

Fetal Membrane ◽

Tissue Adhesives ◽

Amniotic Cavity ◽

Sheep Model ◽

Preterm Premature Rupture ◽

Post Surgery ◽

Pregnant Sheep

Introduction: Iatrogenic preterm premature rupture of the membrane remains the Achille’s heel of fetoscopy. The aim of this study was to show in vivo feasibility of fetal membrane (FM) defect sealing by the application of tissue glues with umbrella-shaped receptors. Methods: First, we adapted our previously described ex vivo strategy and evaluated the adhesion strength of different tissue glues, Histoacryl® and Glubran2®, by bonding polytetrafluoroethylene or silicone encapsulated nitinol glue receptor to human FM. Then, we exposed pregnant sheep uterus through a laparotomy and placed a 10-French trocar into the amniotic cavity through which the umbrella-shaped glue receptor (n = 9) was inserted and fixated onto the FM with the tissue glues (n = 8). The tightness of the sealed defects was assessed 4 h post-surgery. Results: Both tissue glues tested resulted in adhesion of the glue receptors to the FM ex vivo. In vivo, all glue receptors opened in the amniotic cavity (n = 9) and all successfully placed glue receptors sealed the FM defect (n = 8). Four hours post-surgery, 2 treatment sites showed minimal leakage whereas the negative control without glue (n = 1) showed substantial leakage. Discussion: This in vivo study confirms that fetoscopically induced FM defects can be sealed by the application of tissue adhesives.

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