Structure and specificity of a class II MHC alloreactive gamma delta T cell receptor heterodimer

T lymphocytes bearing the gamma/delta T cell receptor (TCR-gamma/delta) express a limited number of germline variable gene segments, generating receptor sequence diversity primarily through junctional mechanisms. To examine the role of V(D)J junctional sequences in antigen recognition by TCR-gamma/delta, we derived an alloreactive murine TCR-gamma/delta+ T cell line, LKD1, specific for the I-Ad class II major histocompatibility complex (MHC) molecule, and compared its receptor with that expressed by a previously characterized class II MHC alloreactive T cell line, LBK5, specific for I-Ek,b,s Ia molecules. Both LKD1 and LBK5 express receptors encoded by rearranged V gamma 1.2J gamma 2 and V delta 5D delta 2J delta 1 gene elements, differing in sequence only in the V(D)J junctional regions of the gamma and delta genes. These results demonstrate that junctionally encoded sequences corresponding to the putative third complementarity determining region can influence the antigen specificity of TCR-gamma/delta.

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Amino acid substitutions in the floor of the putative antigen-binding site of H-2T22 affect recognition by a gamma delta T-cell receptor

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.90.23.11396 ◽

1993 ◽

Vol 90 (23) ◽

pp. 11396-11400 ◽

Cited By ~ 15

Author(s):

S Moriwaki ◽

B S Korn ◽

Y Ichikawa ◽

L van Kaer ◽

S Tonegawa

Keyword(s):

T Cell ◽

T Cell Receptor ◽

Cell Receptor ◽

Peptide Transporter ◽

Affect Recognition ◽

Antigen Binding ◽

In Vitro Mutagenesis ◽

Gamma Delta ◽

T Cell Clone ◽

Gamma Delta T Cell

We have previously identified a self-reactive gamma delta T-cell clone (KN6) specific for the H-2T region gene product T22b. Now we have investigated by an in vitro mutagenesis analysis of the T22b gene the possibility that the interaction between the KN6 gamma delta T-cell receptor and T22b involves a peptide. The results demonstrate that mutations at the floor of the putative antigen-binding groove of T22b affect recognition by the gamma delta T-cell receptor. Furthermore, we have shown that KN6 cells react with cells that are deficient in the class I peptide transporter TAP1/TAP2. These results suggest that peptide is involved in the interaction of the KN6 T-cell receptor with T22 and that loading of T22 with the putative peptide is TAP1/TAP2-independent.

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Gamma/delta lineage relationship within a consecutive series of human precursor T-cell neoplasms

Blood ◽

10.1182/blood.v74.7.2508.2508 ◽

1989 ◽

Vol 74 (7) ◽

pp. 2508-2518 ◽

Cited By ~ 28

Author(s):

JP de Villartay ◽

AB Pullman ◽

R Andrade ◽

E Tschachler ◽

O Colamenici ◽

...

Keyword(s):

T Cell ◽

T Cell Receptor ◽

Cell Receptor ◽

Consecutive Series ◽

Gene Rearrangements ◽

Gamma Delta ◽

Delta Gene ◽

Gene Usage ◽

Rearrangement Process ◽

Gamma Delta T Cell

Abstract We analyzed the gene rearrangements associated with the newly described delta T-cell receptor (TCR) gene from a series of 19 consecutive precursor T-cell (lymphoblastic) neoplasms that represent discrete stages surrounding the TCR gene rearrangement process. Significantly, the delta TCR gene showed rearrangement in most (13 of 19) of these T cells, and in addition it was rearranged in two cells displaying no rearrangement for any other TCR gene. Our survey showed three types of delta gene rearrangements associated with cell-surface TCR expression that presumably represent usage of three V delta genes. This analysis demonstrates (1) a major subclass of human precursor T-cell neoplasms belonging to the gamma/delta T-cell receptor-rearranging subtype; (2) a narrow repertoire of human V delta gene usage; and (3) the utility of delta gene rearrangements as a diagnostic clonal marker in precursor T lymphoblastic neoplasms.

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