The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation

Science ◽  
1988 ◽  
Vol 239 (4840) ◽  
pp. 645-647 ◽  
Author(s):  
M. Sklar
Keyword(s):  
Ionizing Radiation ◽  
3T3 Cells ◽  
Intrinsic Resistance ◽  
Ras Oncogenes ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

Activation of c-myc and c-K-ras oncogenes in primary rat tumors induced by ionizing radiation

1987 ◽  
Vol 7 (2) ◽  
pp. 932-935
Author(s):  
M J Sawey ◽  
A T Hood ◽  
F J Burns ◽  
S J Garte
Keyword(s):  
Ionizing Radiation ◽  
Tissue Specificity ◽  
3T3 Cells ◽  
Rat Skin ◽  
Oncogene Activation ◽  
Myc Gene ◽  
Ras Oncogenes ◽  
Restriction Polymorphisms ◽  
Rat Tumors ◽  
Nih 3T3

An activated K-ras oncogene was detected by transfection in NIH 3T3 cells and by Southern blot analysis in 6 of 12 rat skin tumors induced by ionizing radiation. The DNA from 10 of the 12 tumors also showed c-myc gene amplification and restriction polymorphisms. Evidence for tissue specificity was observed in patterns of oncogene activation, with each of three clear cell carcinomas exhibiting activation of both c-myc and K-ras oncogenes.

scholarly journals NIH/3T3 cells transfected with human tumor DNA containing activated ras oncogenes express the metastatic phenotype in nude mice.

1985 ◽  
Vol 5 (1) ◽  
pp. 259-262 ◽  
Author(s):  
U P Thorgeirsson ◽  
T Turpeenniemi-Hujanen ◽  
J E Williams ◽  
E H Westin ◽  
C A Heilman ◽  
...  
Keyword(s):  
Nude Mice ◽  
Dna Sequences ◽  
Immune Cell ◽  
Human Tumor ◽  
3T3 Cells ◽  
Metastatic Phenotype ◽  
Ras Oncogenes ◽  
Nih 3T3 ◽  
Tumor Dna ◽  
Nih 3T3 Cells

NIH/3T3 cells transfected with DNA from malignant human tumors produced experimental and spontaneous metastases in nude mice. In contrast, parent or spontaneously transformed NIH/3T3 cells failed to metastasize. The transfected clones contained either activated c-Harvey-ras or N-ras oncogenes. A representative clone (T71-17SA2) which was used to assess selected cellular and host factors relevant to the metastatic process produced lung metastases in 100% of the NIH nude mice recipients, secreted augmented levels of type IV collagenase, and invaded human amnion basement membrane in vitro. Expression of the metastatic phenotype was not related to decreased sensitivity to natural killer cells or macrophage-mediated cytotoxicity. Analysis of the cellular DNA from the T71-17SA2 transfectant and its corresponding metastases, both of which contained activated N-ras oncogenes, revealed a twofold increase in the N-ras-specific DNA sequences in the metastatic cells. Thus, transfection with human tumor DNA containing activated ras oncogenes can induce the complete metastatic phenotype in NIH/3T3 cells by a mechanism apparently unrelated to immune cell killing.

NIH/3T3 cells transfected with human tumor DNA containing activated ras oncogenes express the metastatic phenotype in nude mice

1985 ◽  
Vol 5 (1) ◽  
pp. 259-262
Author(s):  
U P Thorgeirsson ◽  
T Turpeenniemi-Hujanen ◽  
J E Williams ◽  
E H Westin ◽  
C A Heilman ◽  
...  
Keyword(s):  
Nude Mice ◽  
Dna Sequences ◽  
Immune Cell ◽  
Human Tumor ◽  
3T3 Cells ◽  
Metastatic Phenotype ◽  
Ras Oncogenes ◽  
Nih 3T3 ◽  
Tumor Dna ◽  
Nih 3T3 Cells

NIH/3T3 cells transfected with DNA from malignant human tumors produced experimental and spontaneous metastases in nude mice. In contrast, parent or spontaneously transformed NIH/3T3 cells failed to metastasize. The transfected clones contained either activated c-Harvey-ras or N-ras oncogenes. A representative clone (T71-17SA2) which was used to assess selected cellular and host factors relevant to the metastatic process produced lung metastases in 100% of the NIH nude mice recipients, secreted augmented levels of type IV collagenase, and invaded human amnion basement membrane in vitro. Expression of the metastatic phenotype was not related to decreased sensitivity to natural killer cells or macrophage-mediated cytotoxicity. Analysis of the cellular DNA from the T71-17SA2 transfectant and its corresponding metastases, both of which contained activated N-ras oncogenes, revealed a twofold increase in the N-ras-specific DNA sequences in the metastatic cells. Thus, transfection with human tumor DNA containing activated ras oncogenes can induce the complete metastatic phenotype in NIH/3T3 cells by a mechanism apparently unrelated to immune cell killing.

scholarly journals Activation of c-myc and c-K-ras oncogenes in primary rat tumors induced by ionizing radiation.

1987 ◽  
Vol 7 (2) ◽  
pp. 932-935 ◽  
Author(s):  
M J Sawey ◽  
A T Hood ◽  
F J Burns ◽  
S J Garte
Keyword(s):  
Ionizing Radiation ◽  
Tissue Specificity ◽  
3T3 Cells ◽  
Rat Skin ◽  
Oncogene Activation ◽  
Myc Gene ◽  
Ras Oncogenes ◽  
Restriction Polymorphisms ◽  
Rat Tumors ◽  
Nih 3T3

An activated K-ras oncogene was detected by transfection in NIH 3T3 cells and by Southern blot analysis in 6 of 12 rat skin tumors induced by ionizing radiation. The DNA from 10 of the 12 tumors also showed c-myc gene amplification and restriction polymorphisms. Evidence for tissue specificity was observed in patterns of oncogene activation, with each of three clear cell carcinomas exhibiting activation of both c-myc and K-ras oncogenes.

scholarly journals Overexpression of protein kinase C-delta and -epsilon in NIH 3T3 cells induces opposite effects on growth, morphology, anchorage dependence, and tumorigenicity.

1993 ◽  
Vol 268 (9) ◽  
pp. 6090-6096 ◽  
Author(s):  
H. Mischak ◽  
J.A. Goodnight ◽  
W. Kolch ◽  
G. Martiny-Baron ◽  
C. Schaechtle ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
3T3 Cells ◽  
Growth Morphology ◽  
Protein Kinase C Delta ◽  
Kinase C ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

Metabolism of n-6 fatty acids by NIH-3T3 cells transfected with the ras oncogene

1994 ◽  
Vol 139 (1) ◽  
pp. 71-81 ◽  
Author(s):  
R. J. de Antueno ◽  
R. C. Cantrill ◽  
Y-S. Huang ◽  
G. W. Ells ◽  
M. Elliot ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Ras Oncogene ◽  
3T3 Cells ◽  
Nih 3T3 ◽  
Nih 3T3 Cells

scholarly journals Retinoic Acid Down-regulation of Fibronectin and Retinoic Acid Receptor Proteins in NIH-3T3 Cells

1996 ◽  
Vol 271 (11) ◽  
pp. 6502-6508 ◽  
Author(s):  
Giorgio Scita ◽  
Nadine Darwiche ◽  
Eileen Greenwald ◽  
Miriam Rosenberg ◽  
Katerina Politi ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Retinoic Acid Receptor ◽  
3T3 Cells ◽  
Down Regulation ◽  
Acid Receptor ◽  
Receptor Proteins ◽  
Nih 3T3 ◽  
Nih 3T3 Cells
Sign in / Sign up

Export Citation Format

Share Document