Serotypes, Clones, and Mechanisms of Resistance of Erythromycin-Resistant Streptococcus pneumoniae Isolates Collected in Spain

ABSTRACT The aim of this study was to analyze the distributions of antibiotic susceptibility patterns, serotypes, phenotypes, genotypes, and macrolide resistance genes among 125 nonduplicated erythromycin-resistant Streptococcus pneumoniae clinical isolates collected in a Spanish point prevalence study. The prevalence of resistance to macrolides in this study was 34.7%. Multiresistance (to three or more antimicrobials) was observed in 81.6% of these strains. Among 15 antimicrobials studied, cefotaxime, moxifloxacin, telithromycin, and quinupristin-dalfopristin were the most active drugs. The most frequent serotypes of erythromycin-resistant isolates were 19F (25%), 19A (17%), 6B (12%), 14 (10%), and 23F (10%). Of the 125 strains, 109 (87.2%) showed the MLSB phenotype [103 had the erm(B) gene and 6 had both erm(B) and mef(E) genes]. Sixteen (12.8%) strains showed the M phenotype [14 with mef(E) and 2 with mef(A)]. All isolates were tested by PCR for the presence of the int, xis, tnpR, and tnpA genes associated with conjugative transposons (Tn916 family and Tn917). Positive detection of erm(B), tet(M), int, and xis genes related to the Tn916 family was found in 77.1% of MLSB phenotype strains. In 16 strains, only the tndX, erm(B), and tet(M) genes were detected, suggesting the presence of Tn1116, a transposon recently described for Streptococcus pyogenes. Five clones, namely, Sweden15A-25, clone19F ST87, Spain23F-1, Spain6B-2, and clone19A ST276, accounted for half of the MLSB strains. In conclusion, the majority of erythromycin-resistant pneumococci isolated in Spain had the MLSB phenotype, belonged to multiresistant international clones, and carried the erm(B), tet(M), xis, and int genes, suggesting the spread of transposons of the Tn916 family.

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Distribution of macrolide-resistant genes among isolates of macrolideresistant Streptococcus pyogenes and Streptococcus pneumoniae in Serbia

Archives of Biological Sciences ◽

10.2298/abs1401093g ◽

2014 ◽

Vol 66 (1) ◽

pp. 93-98 ◽

Cited By ~ 2

Author(s):

Ina Gajic ◽

Vera Mijac ◽

Natasa Opavski ◽

Maja Stanojevic ◽

Ivana Lazarevic ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Streptococcus Pyogenes ◽

Resistance Genes ◽

Macrolide Resistance ◽

Mechanisms Of Resistance ◽

Diffusion Test ◽

Disk Diffusion Test ◽

Resistant Genes ◽

Group A ◽

Ermb Gene

Macrolide resistance in Streptococcus pneumoniae and in group A streptococci (GAS) is a significant problem worldwide. In Serbia, data on the mechanisms of resistance and the corresponding resistance genes in streptococci are largely lacking. Therefore, we analyzed the distribution of macrolide resistance phenotypes and genotypes in 44 macrolideresistant GAS (MRGAS) and 50 macrolide-resistant S. pneumoniae (MRSP) isolates collected in the same period. The double disk diffusion test and PCR were used to analyze resistance phenotypes and resistance genes, respectively. Among MRSP, the MLSB phenotype dominated, whereas the M phenotype was the most prevalent among MRGAS isolates. Consequently, in MRSP, the ermB gene was the most common (n=40, 80%), followed by the mefA gene (n=7,14%). In MRGAS strains, mefA dominated (n=27,61%), followed by ermA (n=15, 33%) and ermB (n=3, 7%). In 3 MRSP isolates no resistance genes were detected, while one MRGAS strain with iMLSB phenotype harbored both ermA and mefA genes.

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Streptococcus pneumoniae Serotypes and Antibiotic Susceptibility Patterns in Middle Ear Fluid Isolates During Acute Otitis Media and Nasopharyngeal Isolates During Community-acquired Alveolar Pneumonia in Central Romania

The Pediatric Infectious Disease Journal ◽

10.1097/inf.0000000000001379 ◽

2017 ◽

Vol 36 (2) ◽

pp. 151-154 ◽

Cited By ~ 5

Author(s):

Raluca-Ileana Lixandru ◽

Cristian Falup-Pecurariu ◽

Laura Bleotu ◽

Alice Mercas ◽

Eugene Leibovitz ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Otitis Media ◽

Middle Ear ◽

Antibiotic Susceptibility ◽

Acute Otitis Media ◽

Middle Ear Fluid ◽

Antibiotic Susceptibility Patterns ◽

Susceptibility Patterns

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Antibiotic susceptibility patterns and resistance genes of starter cultures and probiotic bacteria used in food

Systematic and Applied Microbiology ◽

10.1016/j.syapm.2005.07.009 ◽

2006 ◽

Vol 29 (2) ◽

pp. 145-155 ◽

Cited By ~ 114

Author(s):

Sabine Kastner ◽

Vincent Perreten ◽

Helen Bleuler ◽

Gabriel Hugenschmidt ◽

Christophe Lacroix ◽

...

Keyword(s):

Resistance Genes ◽

Antibiotic Susceptibility ◽

Starter Cultures ◽

Probiotic Bacteria ◽

Antibiotic Susceptibility Patterns ◽

Susceptibility Patterns

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A point prevalence survey of antimicrobial agents and antibiotic susceptibility patterns of admitted patients in acute wards of a teaching hospital in Western India

Indian Journal of Pharmacy and Pharmacology ◽

10.18231/j.ijpp.2019.020 ◽

2019 ◽

Vol 6 (3) ◽

pp. 86-92

Author(s):

Sharan Shyam ◽

◽

Sanjay Jaiswal ◽

Keyword(s):

Teaching Hospital ◽

Antibiotic Susceptibility ◽

Antimicrobial Agents ◽

Point Prevalence ◽

Western India ◽

Prevalence Survey ◽

Point Prevalence Survey ◽

Antibiotic Susceptibility Patterns ◽

Susceptibility Patterns ◽

Acute Wards

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Antibiotic Susceptibility Patterns ofStreptococcus pneumoniae in China and Comparison of MICs by Agar Dilution and E-Test Methods

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.42.10.2633 ◽

1998 ◽

Vol 42 (10) ◽

pp. 2633-2636 ◽

Cited By ~ 18

Author(s):

Hui Wang ◽

Robin Huebner ◽

Minjun Chen ◽

Keith Klugman

Keyword(s):

Streptococcus Pneumoniae ◽

Clavulanic Acid ◽

Antibiotic Susceptibility ◽

Test Methods ◽

Beta Lactam ◽

Beta Lactams ◽

Amoxicillin Clavulanic Acid ◽

Agar Dilution ◽

Antibiotic Susceptibility Patterns ◽

Susceptibility Patterns

ABSTRACT Beta-lactam resistance by Streptococcus pneumoniae is becoming a significant threat to public health worldwide. However, data concerning antibiotic susceptibility patterns in China have not been published. In this study, a total of 79 clinical isolates and 244 nasopharyngeal isolates of S. pneumoniae were recovered between June and November 1997 in Beijing. The agreement between the MICs (±1 log2 dilution) of penicillin and ceftriaxone obtained by the agar dilution and E-test methods for the 79 clinical strains was very good (97.5 and 93.7%, respectively). Of these 79 strains, 9 (11.4%) were intermediate and 2 (2.5%) were resistant to penicillin. Of the 244 nasopharyngeal strains, 32 (13.1%) were intermediate and 3 (1.2%) were resistant to penicillin. The total of 277 penicillin-susceptible clinical and nasopharyngeal isolates ofStreptococcus pneumoniae were 100% susceptible to amoxicillin-clavulanic acid, cefuroxime, ceftriaxone, and cefotaxime. In the 35 penicillin-intermediate and -resistant nasopharyngeal strains, elevated MICs of amoxicillin-clavulanic acid, cefuroxime, ceftriaxone, and cefotaxime were seen for ≤4 isolates. Of 244 nasopharyngeal isolates, the overall percentages of tetracycline, erythromycin, chloramphenicol, ofloxacin, and trimethoprim-sulfamethoxazole resistance were 87.6, 74.0, 47.8, 3.7 and 63.3, respectively. Vancomycin and rifampin resistance were not detected. These findings demonstrate that the rate of penicillin-resistant pneumococci is relatively low in China compared to those of other Asian countries. Resistance to non-beta-lactams was much higher than to beta-lactams. The E-test and agar dilution methods appeared to be comparable in identifying resistant strains.

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Serotype incidence and antibiotic susceptibility of Streptococcus pneumoniae causing invasive disease in Scotland, 1999–2002

Journal of Medical Microbiology ◽

10.1099/jmm.0.45718-0 ◽

2005 ◽

Vol 54 (4) ◽

pp. 327-331 ◽

Cited By ~ 14

Author(s):

B C Denham ◽

S C Clarke

Keyword(s):

Streptococcus Pneumoniae ◽

Antibiotic Susceptibility ◽

Pneumococcal Disease ◽

Invasive Disease ◽

Reference Laboratory ◽

Conjugate Vaccines ◽

Non Invasive ◽

Antibiotic Susceptibility Patterns ◽

The Uk ◽

Susceptibility Patterns

Pneumococcal disease remains an important cause of invasive and non-invasive disease in Scotland and elsewhere. The Scottish Meningococcus and Pneumococcus Reference Laboratory receives isolates of Streptococcus pneumoniae from diagnostic laboratories around Scotland. Here, the serogroups/types and antibiotic-susceptibility patterns of invasive isolates received between 1999 and 2002 are described. There were a total of 1741 invasive isolates received, the most common serogroups/types being 14 (19.8 %), 9 (10.2 %), 6 (8.3 %), 19 (7.9 %), 23 (7.9 %), 4 (6.5 %), 8 (6.4 %), 3 (5.7 %), 1 (3.8 %), 7 (3.8 %) and 18 (3.4 %). Importantly, serotypes 7 and 8 are not represented in the 7-, 9- and 11-valent pneumococcal conjugate polysaccharide vaccines. There were 67 (3.8 %) isolates considered penicillin non-susceptible, although no penicillin resistance (MIC ⩾ 0.002 mg ml−1) was recorded. One hundred and ninety-four (11.1 %) isolates, predominantly of serotype 14, were resistant to erythromycin, and 12 (0.7 %) were resistant to ciprofloxacin. This information provides an important dataset that will prove essential prior to and during the implementation of pneumococcal conjugate vaccines in the UK.

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